1.Feeder-free growth of human embryonic stem cells supported by basic fibroblast growth factor
Chinese Journal of Tissue Engineering Research 2010;14(6):1111-1114
BACKGROUND: Human embryonic stem cells (hESCs) are pluripotent cells which may differentiate into tissues of all three germ layers. Such research as the feeder-free growth of hESCs is few in China. Fibroblast growth factor (FGF) is a major factor to maintain the undifferentiated state of hESCs.OBJECTIVE: To evaluate the ability of FGF at different concentrations in maintaining the undifferentiated state and pluripotency of hESC lines in the long-term culture.METHODS: Two cell lines of hES-8 and hES-18 were cultured with mouse embryonic fibroblast condition medium for 3 passages and then transferred into mouse embryonic fibroblast condition medium containing different concentrations of FGF: 100, 160, 250 μg/L for 8 passages. The hESCs were removed from the petri dish, cell clusters were digested with collagenase IV and gathered. Cell differentiation and pluripotency were observed. The eighth generation of the hESCs were collected and incubated into severe combined immunodeficiency mice, so as to observe teratoma formation. Morphologies of the cells were evaluated. Alkaline phosphatase staining, surface labeling immunocytochemical analysis and RT-PCR assay method were utilized to determine the OCT-4 expression and tumorigenesis in vivo.RESULTS AND CONCLUSION: Cultured in mouse embryonic fibroblast condition medium containing 160 and 250 μg/L FGF, two cell lines of hESC could maintain undifferentiated state: Clones were round with a high ratio of nucleus to cytoplasm. Large areas in the center of clones were undifferentiated cells, while surrounding the clones were differentiated cells; Strong positive expression for alkaline phosphatase staining was observed; Two cell lines showed high levels of OCT-4 transcription factor protein; The surface markers SSEA-4, TRA-1-60, TRA-1-81 were all positive on both two lines; The hESC clusters could form embryoid body in vivo 10 days later; 3 germ layers of teratomas were also obtained after implanted into severe combined immunodeficiency mice. Mouse embryonic fibroblast condition medium containing 100 μg/L FGF was not sufficient to maintain the long-term proliferation of hESCs, and most of the cells differentiated and died after 4 passages. Alone with concentration 160 μg/LbFGF or more could maintain two hESC lines undifferentiated stably in vitro, has no influence on the differentiation and totipotency of two cell lines.
2.Ongoing reform and thoughts of the corporate governance experiment at public hospitals in China
Chinese Journal of Hospital Administration 2016;32(10):725-728
Based on a review of literature and the current reform of public hospital corporate governance,such corporate governance is divided intocombined regulation and operation,anddivided regulation and operationmodels.Then the principal-agent theory was called into play to analyze the main problems and causes of the existing models.From the perspectives of government supervision and assessment,professionalization of hospital directors,market competition mechanism,thoughts were made on how to improve such corporate governance.
3.CLINICAL SIGNIFICANCE OF MEASUREMENT OF SERUM PROLACTIN IN PATIENTS WITH RENAL FAILURE
Huifang WANG ; Xueliang FENG ; Hui XU
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
In this article, we observed the serum level of Prolactin and the intensity of renal insufficiency in 40 patients with renal failure and compared their relationship. Our study showed that the serum level of Prolactin in patients with renal failure was much higher than that of normal controls (P
4.CT and MRI manifestations of intracranial gliosarcoma
Leili ZHANG ; Shimin CUI ; Huifang XU
Chinese Journal of Radiology 2001;0(05):-
Objective To investigate the clinical and characteristic imaging findings of intracranial gliosarcoma. Methods The imaging findings of surgery and pathology proved intracranial gliosarcoma in 15 cases were retrospectively analyzed. There were 10 males and 5 females, ranging in age from 44 to 67 years (mean age 58 years). Results All tumors were located in supratentorium, including 13 cases in the temporal or parietal lobe, 1 cases in the occipital lobe, and 1 cases in the thalamus. Tumors appeared as round or ellipse in shape. The lesions ranged in size from 3 to 9 cm (mean 4.6 cm). CT scan showed mingled hypo and isodensity in 7, hypodense in 4 with CT value from 18 to 22 HU, hyperdense in 4 with the CT value from 48 to 55 HU. Enhancement appeared as an irregular pattern. The margin was clear. Most intracranial gliosarcoma showed perifocal edema. MRI were examined in 6 cases, whichappeared as low signal on T 1WI, and as high signal on T 2WI. There was irregular enhancement of the mass. Conclusion It was difficult to make the correct preoperative diagnosis for gliosarcoma, but it was possible to make a tendency diagnosis using CT or MR combined with the history. Contrast enhanced CT and MRI should be useful in differentiating gliosarcoma from glioblastoma.
5.Factors affecting the transfer of ropivacalne across the single cotyledon of term human placenta
Aizhong WANG ; Wei JIANG ; Huifang XU
Chinese Journal of Anesthesiology 1996;0(07):-
Objective To study the transfer of ropivacaine across the single cotyledon of the term human placenta and the effects of maternal hypoproteinemia and fetal acidemia on the transfer. Methods Eighteen placentas were obtained from healthy full term parturients within 5 min after vaginal or cesarean section delivery. The dual perfused human placental model was made. The placentas were randomly divided into three groups of 6 placentas : (A) control group in which 100% fresh frozen plasma was used in both maternal and fetal circulation with pH maintained at 7.4 on both sides; (B) fetal academia group in which 100% fresh frozen plasma was used in both circulations but fetal pH was reduced to 7.0; (C) maternal hypoproteinemia group in which 50% fresh frozen plasma used in maternal circulation and 100% fresh frozen plasma in fetal circulation, pH was maintained at 7.4 on both sides. Samples were taken from the perfusate in the reservoir at 15, 30, 60, 90, 120 min after ropivacaine (2?g?ml-1) and antipyrine (10 ?g?ml-1 ) were added in maternal circulation for determination of concentrations of ropivacaine, antipyrine, glucose and lactate. Glucose consumption rate, lactate generation rate and relative and absolute transfer ratio of ropivacaine were calculated. Results Absolute transfer ratio of ropivacaine was gradually increasing with perfusion time, reaching 8.7?1.0% (A) , 10.5 ?1.6% (B) and 11.8?1.1% respectively at 120 min. Relative transfer ratio of ropivacaine was relatively constant during 120 min perfusion and was significantly higher at each time point in group B and C than in group A ( P
6.Protective effect of phospholipase A_2 inhibitor on vital organs injury from intestinal ischemia-reperfusion
Gang ZHAO ; Huifang XU ; Wei JIANG
Chinese Journal of Anesthesiology 1996;0(09):-
Phospholipase A_2 (PLA_2) inhibitor quinacrine was used to explore protective effect on multiple organ dysfunction (MOD) caused by intestinal ischemia-reperfusion (I/R) in rats. Gut I/R caused the increase of gut PLA_2 activity and induced endotoxemia and bacteriemia. Pretreatment with intravenous quinacrine 10mg?kg~(-1) attenuated bacteria and endotoxin translocation,markedly lowered the levels of thromboxane A_2 and prostacyclin I_2 in blood,and provided protection from the development of vital organs dysfunction. As a result,the survival rate in pretreatment group increased by 25%. The results demonstrate that gut I/R promotes gut barrier failure,then contributes to the development of MOD by allowing bacteria or endotoxin reaching the circulation. PLA_2 and PLA_2-dependent lipid mediators play an important role in the development of gut I/R injury and MOD. Intravenous quinacrine has protection against MOD resulting from gut I/R.
7.Experimental study of changes of intestinal immunity following traumatic shock and resuscitation
Xia LIU ; Huifang XU ; Wei JIANG
Chinese Journal of Anesthesiology 1994;0(03):-
Objective: We investigated experimentally the changes of intestinal immunity following shock and resuscitation after trauma. Method:The experimental model was made in rats, which underwent laparotomy, then bleeding and reinfusing through the right fetmoral artery. Result: The concentration of IgA following shock and resuscitation were significantly higher than that before shock.. The concentration of IgA 24 h following resuscitation was the lowest, and was significantly lower than that at the end of shock.. The endotoxin in portal vein following shock and resuscitation were higher than that before shock.. The endotoxin level 24 h following resuscitation was the highest, and markedly higher than that at the end of shock. Conclusion: The traumatic shock and resuscitation are capable of causing intestinal immunosuppression and endotoxemia.
8.Clinical assessment of the effect of intravenous PCA and epidural PCA in postoperative analgesia
Xiaoli ZHANG ; Dongping DU ; Huifang XU
Chinese Journal of Anesthesiology 1994;0(04):-
To compare the clinical effect and side-effect of intravenous PCA(PCIA) with epidural PCA (PCEA). Method: One hundred and eighty postoperative patients, who were randomly divided into three groups: group PCIA, group PCEA and control, were observed for 3 days after operation. Result: Overall patients in two PCA groups were satisfied with the postoperative analgesia. The incidence of urinary retention in group PCIA were significantly lower than that in group PCEA (P0.05). 10% in group PCIA were in medium sedation whereas no eases in group PCEA were in sedation. At 6th hour after operation,serum cortisol level of control patients was much higher than that of PCA patients. Conclusion=Both PCIA and PCEA have excellent analgesia and reduce stress response after operation. PClA has lower incidence of urinary retention and is performed easily,but inhibits gastrointestinal motility much more and has higher sedative incidence compared with PCEA.
9.Action of pentoxifylline on oxygen free radicals-induced myocardial injury
Huifang XU ; Li WANG ; Wei JIANG
Chinese Journal of Anesthesiology 1995;0(12):-
Objective: To investigate the effects of pentoxifylline (PTX) on oxygen free radicals induced myocardial injury. Method: An in vivo myocardial impairment model was developed in SD rat with exogenous oxygen free radicals. The changes cardiac performance and serum malonylaldehyde (MDA), lactic acid, cretine phosphokinase (CK) levels were tested before and at different interval after I. V. administration of oxygen free redieal. Result: Myocardial performance after oxygen free radicals administration was significantly depressed compared to the baseline values (P
10.Effects of exogenous nitric oxide on the alveolar macrophage function in the rats with acute lung injury
Xuemin WANG ; Huifang XU ; Wei JIANG
Chinese Journal of Anesthesiology 1994;0(01):-
0.05 ),the O- 2 production increased significantly (P