Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Heat Stress Disorders ; drug therapy ; metabolism ; Hypothalamus ; metabolism ; Male ; Neurotensin ; metabolism ; Phytotherapy ; Rats ; Rats, Wistar ; Tablets

Circulating tumor cells are closely related to the development and progression of malignant tumors. Current methods of detecting circulating tumor cells include reverse transcription polymerase chain reaction method and immunomagnetic enrichment assay. Detection of circulating tumor cells can contribute to early detection of cancer micrometastases, re-classification of clinical stage, monitoring recurrence and metastasis after surgery or radiotherapy and chemotherapy, predicting prognosis, and selecting personalized therapeutic strategy.

Physiological and biochemical characteristics,drug sensitivity of pathogenic bacteria isolated from the diseased Sparus latus in Zhanjiang,Guangdong,and inactivated whole cell vaccine were studied.The results show that the isolated strain is Vibrio alginolyticus and is highly sensitive to tetracycline,gentamycin,chloramphenicol,and so on.But it is not sensitive to erythromycin and penicillin K.It also shows that the pathogen is inactivated more easily at pH6.0 than at pH8.4 when formalin is used.The whole cell vaccine can strengthen Sparus latus's immuno function and lessen their death rate when fish are infected by the Vibrio alginolyticus.

The training of clinical skill is closely linked with medical personnel education.However,traditional paper-type training materials were unable to meet the training needs.As a new type of training materials,trial-and-error video-type clinical skill training materials (abbreviated as TEVT clinical skill training materials) have gradually attracted attention with its characteristics of lively and interesting,potentially informative,high acceptance among students,the advantages' of stimulating students' initiative exploration and consideration,fostering students' critical spirit and humanities.On the basis of analyzing the advantages' of TEVT training materials,we discussed the developing specialties of TEVT clinical skill training materials from four aspects as the material orientation and the application object,material sources,constructing the developing team and editing the teaching materials.Furthermore,we reflected the application prospect of TEVT clinical skill training materials in the future,in order to explore an available implementation path for the development of TEVT clinical skill training materials.

Objective To characterize the medial femoral circumflex artery based on three-dimensional computed tomographic angiography (3DCTA) so as to provide anatomical aids for diagnosis and treatment of hip injuries.Methods To do this research the 3DCTA data of 103 patients (206 hips) were collected who underwent 3DCTA from March 2012 to June 2014 for examination of the pelvis and lower extremities.They were 55 men (mean age,54.7 ± 15.9 years) and 48 women (50.0 ± 16.1 years).① The origin and course of the medial femoral circumflex artery was observed.② The length of the artery trunk was measured.The distances between the origin(A) of the artery to the main anatomical marks [the peak of greater trochanter (B),the inward peak of lesser trochanter (C),and the crosspoint (D) of the femoral artery and the pubic edge or the acetabulum inlet edge] were measured.Comparisons of the measurements were conducted between genders.③ The distance between point A and point E,the starting point of the femoral profound artery,was measured.Comparisons of the measurements were conducted between genders.Results 2.9％ (3/103) of the subjects had their bilateral medial femoral circumflex arteries originate from the femoral artery,94.2％ (97/103) had their bilateral medial femoral circumflex arteries originate from the femoral profound artery,and 2.9％ (3/103) had one medial femoral circumflex artery originate from the femoral artery and the other from the femoral profound artery.1.9％ (4/206),76.7％ (158/206) and 21.3％ (44/206) of the subjects had the artery of screw type,of circuitous type and of straight type,respectively.The artery trunk goes posteriorly and medially after it is issued,divides into branches along the course,continues to go around the back of the femoral neck,and finally goes to the outward and upper direction.There were no statistical differences between males and females regarding length of the artery trunk,or distances between points AB,AC,AD or AE (P ＞ 0.05).Conclusion Since 3DCTA can clearly demonstrate the origin,course and anatomical morphology of the medial femoral circumflex artery,it can be used to provide anatomical aids for clinical evaluation and treatment of hip injury.

Objective To investigate the role of endoplasmic reticulum stress ( ERS) in asbestos-induced Alveolar epithelial cell apoptosis.Methods A549 cells were treated with asbestos to observe the expression of ERS proteins and apop-tosis gene by immunofluorescence staining and western blotting.Results Under asbestos exposure, the expression of Bip, GRP94, IRE-1α, BAX and BAK are up-regulated.There is a positive correlativity of the expression of proteins to the ex-posure time of asbestos.Conclusion Endoplasmic reticulum stress takes part in asbestos-induced apoptosis.

BACKGROUND: Metal on metal (MoM) hip prosthesis has been used for more than 30 years. With the increasing number of young patients who used it, the metal ions cumulative in the body may cause the biological effects, such as cell toxicity, genetic toxicity, carcinogenicity and metal allergy, etc, which have received attention and concern. OBJECTIVE: To describe what influences the production of metal ions, and how metal ions distribute in human tissue after MoM hip prosthesis implantation. In particular the change of the metal ions serum level and the MoM hip prosthesis biological effects of the pathological manifestations were investigated. This paper also focused on the factors influencing metal ions serum level and the mechanism of metal ions biological effects. RETRIEVAL STRATEGY: A computer-based search of Pubmed database was undertaken to identify the articles between January 2004 and November 2007, with the key items of "metals, total hip replacement" and language was limited to English. Meanwhile the China Journal Full-text Database was retrieved for the related articles published from January 2000 to November 2007, with the words of "joint replacement, metal ions" in Chinese. Totally 267 articles were screened out, and checked primarily. Inclusion criteria: ①prosthesis interface described the articles for MoM;②procedure described in the articles for hip replacement;③ articles focused on biological effects of metal ions. Exclusion criteria: ①non-MoM interface of the prosthesis;②non-hip arthroplasty;③articles described the metallurgy;④reproducibility study. LITERATURE EVALUATION: Both titles and abstracts were screened preliminarily, excluding 194 articles of unrelated objectives, 58 articles of repeated contents and 0 Meta analysis. Finally 30 articles were taken into further analysis, including 6 reviews and other basic or clinical trials. DATA SYNTHESIS: MoM prosthesis releases metal ions in the body through electrochemical process (corrosion) and enhanced machinery plus electrochemical process (wear-corrosion). It spreads to various body tissues by body fluid, and raises a number of biological effects. Human body shows a reaction of metal ions in a dose-dependent manner, and metal ions serum level decides the biological effects. Generally we consider the serum concentration of metal ions is influenced by ①ion excretion factors. ② mechanical factors. ③individual factors. ④others. At present, the biological effects of metal ions include ①inflammatory response, ②oxidative damage, ③apoptosis, ④osteoporosis and extracellular matrix dissolution, ⑤carcinogenesis, ⑥host response. Its mechanism is related with corrosion, macrophage activation, reactive oxygen generation, osteoblast function inhibition, osteoclast activation, metal-protein complex formation, and so on. The consequence is a series of pathological changes: ①foreign body granuloma synovitis, ②draining lymph node lesions, ③chromium calmly disease, ④malignant lesions, ⑤metal allergy. CONCLUSION: There is no better product to replace MoM hip prosthesis. Through the study of their biological effects, people gradually concern about two issues. First: What is a safe wear rate? Second: What is harmful serum level of metal ions?

Objective To study the changes of expression levels of vascular endothelial growth factor(VEGF) and its receptors(FLT-1,FLK-1) mRNA in equal area of penumbra in diabetes rats with cerebral infarction(DMCI).Methods Middle cerebral artery occlusion(MACO) model was made in diabetes rats.The expression levels of VEGF and FLT-1,FLK-1 mRNA in equal area of penumbra were measured with situ hybridization in different times after MACO(1 h,3 h,6 h,12 h,24 h).The difference was compared between DMCI group and no diabetes cerebral infarction(NDMCI) group.Results The expression level of VEGF mRNA was lower in DMCI group than that of NDMCI group at every time point(P

Restenosis is a major complication after percutaneous transluminal coronary intervention. Evaluation of restenosis early and accurately can help to giude treatment, improve outcome and save costs for the patients. All kinds of imaging methods for restenosis have their merits and demerits. It is essential to choose the appropriate imaging method in clinical practice.

Objective To study the analgesic effect of sufentanil combined with Dezocine in endoscopic resection of fallopian.MethodsA total of 188 patients with tubal pregnancy from April 2013 to December 2014 in Haining central hospital were enrolled in the study, they were randomly divided into two groups (n=94).On the basis of conventional intravenous analgesia, the patients in control group was were received sufentan, the observation group were given sufentan combined with dezocine.The score of pain at different time points after operation, the Ramsay sedation score, and the level of IL-6 and IL-10 were compared.The adverse reactions were compared between the two groups.ResultsThe score of VAS in the observation group was significantly lower than that in the control at 4h, 8h, 12h and 24h after operation (P<0.05).The score of VAS in the two groups were similar at 48h after operation;the Ramsay score in the observation group was significantly higher than that in the control group(P<0.05) at five time points (4h, 8h, 12h, 24h and 48h after operation);when compared Compared with the indexes before operation, the level of IL-6 was significantly increased in the two groups at 24h and 48h, but the observation group increased less, the IL-6 in observation group was lower than that in the control group at the two points(P<0.05);while the IL-10 was higher than that beforeoperation, and the observation group was more higher(P<0.05).The adverse reactions in the two groups were mainly dizziness, nausea, pruritus and lethargy, and the incidence was only 12.8% in the observation group, which lower than that in the control of 29.8%(P<0.05).ConclusionIt which dazocine combined with sufentanil had a good analgesic effect in the process of analgesia in gynecological laparoscopic resection of fallopian tube after the operation.The VAS score and Ramsay sedation score was improved significantly.The levels of inflammatory factors were lower, and the incidence of adverse reaction was significantly reduced.It is a reliable method for clinical intravenous analgesia.