1.AN Approach to the Morphology of Buccal Armature of Sergentomyia nankingensis
Guanghua XIONG ; Changfa JIN ;
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(05):-
ObjectiveTo describe the morphological variation of pointed teeth of buccal armature of Sergentomyia nankingensis in a supplementary study. Methods The morphology of Sergentomyia nankingensis was observed and the specimens included S nankingensis captured from the field as well as the female ones bred in the laboratary and their offspring of first generation. Results The buccal armature of females and males, consists of about 10-19 and 8-15 pointed teeth and the pigmented plate is polymorphous. In the nature,the number of pointed teeth of buccal armature of Sergentomyia is unstable,varying in more than 10 teeth in the same species sometimes. Conclusion Identification of species needs large amount of specimens for comparative observation. Sergentomyia sandfly should be raised separately and individually for morphological observation of its newly emerged offspring, especially when the sandfly specimens and data collected in the field were not sufficient.
2.IL-13Rα2 as a functional receptor mediates signal transduction
Chinese Journal of Pathophysiology 2014;(8):1513-1518
[ABSTRACT]IL-13isapleiotropiccytokinemainlysecretedbyactivatedTh2cells.Ithas2receptors,IL-13Rα1 and IL-13Rα2.The latter had been thought to serve exclusively as a decoy receptor for a long period of time due to its short cytoplasmic tail and lack of signal transduction structure .Since Fichtner-Feigl reported in Nature Medicine that IL-13 is in-volved in induction of TGF-βproduction and tissue fibrosis through IL-13Rα2-mediated signaling pathway , it was found that IL-13Rα2 has more sophisticated functions than just a simple decoy receptor as more and more researches have explored its signaling functions .This review combines the most advanced research results with previous investigation and discusses the gene structure, expression, production, distribution, subtype conversion and possible signal pathways mediated by this re-ceptor.More importantly, the connection with human diseases and the applications in disease diagnosis and molecule targe -ted therapy for cancer are also discussed .
3.Research Progress on the Resting Habits of Major Sandflies and Control Strategy
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(04):-
Based on the comprehensive studies in the past years, the authors reviewed important findings on biology, especially the resting habits, of the four major species of sandflies transmitting visceral leishmaniasis. The effective ways for sandfly control were also discussed.
4.Preparation of chondroitin sulfate with different molecular weight and antioxitation capacity
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To explore in vitro antioxidan activity of ChS extracted by two enzyme hydrolyses. METHODS: Papain,sodium hydroxide-trypsin were used to extract chondroint sulfate, respectively,DEAE-52 and DEAE-Sepharose FF ion exchange column chromatography were used to isolate and purify crude polysaccharide.RESULTS: The results showed that extraction rate of papain,sodium hydroxide-trypsin for chS was (39.51? 0.89 )%,and (21.23?1.65)%,respectively.DEAE-Sepharose FF was better than DEAE-52 for the isolation and purification of crude polysaccharide,two components were separated and purified from crude polysaccharide extracted by two extraction methods.Four components were separated and purified from crude polysaccharide extracted by papain on the DEAE-Sepharose FF.Three components were obtained by sodium hydroxide-trypsin.Molecular weight of ChS extracted by papain,sodium hydroxide-trypsin was 43569 Da,25773 Da,respectively.The DPPH?, ?OHand O~-_2? scavenging activity of the former was better than those of the latter. CONCLUSION: The study shows that the function of the ChS is probably related to extration mode,molecular weight and conformation.
5.Determination of cholic acid and muscone in Jawei Xihuang Soft Capsule
Fen JIN ; Fuliang XIONG ; Dengke XIONG ; Li XIONG ; Yang XIANG
Chinese Traditional Patent Medicine 2010;(3):429-432
AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:21)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.
6.Determination of cholic acid and muscone in Jawei Xihuang Soft Capsule
Fen JIN ; Fuliang XIONG ; Dengke XIONG ; Li XIONG ; Yang XIANG
Chinese Traditional Patent Medicine 1992;0(03):-
AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:A C18 column(Kromasil C18,5 ?m,4.6 mm?250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:27) at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40 ℃,carrier gas(N2) pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m?0.32 mm?0.25 ?m) and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL—0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.
9.Clinical features of Marfan syndrome and analysis ofFBN1 gene mutation
Xiangqun JIN ; Zhi XIONG ; Liqiong WANG
Journal of Clinical Pediatrics 2016;34(10):775-778
Objective To explore the clinical features of Marfan syndrome (MFS) and its virulence gene mutation of FBN1.Methods Clinical data of 2 children with MFS were retrospectively analyzed, and pertinent literatures were reviewed. Results Case one was a 1 year and 10 months old boy with a special face, bilateral lower eyelid edema, high palatal arch, slender fingers and toes. A little of moist rales in lung could be heard, and systolic accentuated in apex could be heard too. Echocardiography showed that aortic coronary sinus dilated, aorta and pulmonary artery broadened, left ventricular diverticulum, a small amount of mitral regurgitation,and moderate tricuspid regurgitation. Electrocardiogram showed incomplete right bundle branch block. Gene detection found a c.3037G>A mutation (p.Gly1013Arg) inFBN1. Case two was a 12 years old slender boy with spider-like ifnger/toe, high myopia, 2/6 systolic and diastolic murmur in the ifrst and two auscultation area in aortic valves. Echocardiography showed the aortic sinus signiifcantly broadened, aortic incompetence, mild pulmonary regurgitation and left ventricular enlargement. Gene detection found heterozygous mutation of c.1876G>A (p.Gly626Arg) in FBN1, which has not been reported.Conclusion The diagnosis of MFS can be conifrmed byFBN1 gene detection. A new mutation of c.1876G>A (p.Gly626Arg) was detected.
10.Regulatory Effect of Acupuncture and Moxibustion on the Hematopoiesis of Leukopenia Model in Rats
Jin CUI ; Dingzhu SHEN ; Fangli XIONG
Journal of Acupuncture and Tuina Science 2006;4(2):75-78
Objective: To investigate the effect of acupuncture or moxibustion at acupoint Geshu (BL 17) on raising leukocyte and promoting marrow proliferation. Methods: Wister rats were selected to establish the leukopenia and immuno-suppression model by intraperitoneal injection of cytoxan (CTX). After they were grouped and treated, leukocyte and marrow karyocyte were counted in different phases to evaluate the effect of acupuncture or moxibustion at acupoint Geshu (BL 17) on raising leukocyte and promoting marrow hematopoiesis. Results: Acupuncture or moxibustion at acupoint Geshu (BL 17) and routine western medicine could all significantly raise leukocyte and increase marrow karyocyte. The curative effect was better in moxibustion group than in acupuncture group and in routine western medicine group (P<0.05). Conclusion: Moxibustion at acupoint Geshu (BL 17) can markedly raise peripheral blood leukocyte and marrow karyocyte of the leucopenia model in rats.