Objective: To evaluate the efficacy and toxic reactions of Endostar combined with TP chemo-therapy in the treatment of advanced stage non-small cell lung cancer (NSCLC). Methods: A total of 68 cases of advanced stage NSCLC confirmed by pathohistology or cytology were randomly divided into two treate-ment groups: the Endostar combined with TP chemotherapy group (37 cases) and the simple TP chemothera-py group (31 cases). Patients in the Endostar combined with TP chemotherapy group were treated with TXT 65mg/m~2 on d2 and d9, DDP 75 mg/m~2, d1-4; and endostar 15g, d1-14. Patients in the simple chemotherapy group were treated with TXT and DDP. After 2 weeks, we estimated the recent curative effect and toxic reac-tions according to WHO standard. Results: The total response rates in the two groups were 56.8% and 29.7%, respectively, with a significant difference (X~2=4.08, P<0.05). The main toxic reactions were bone marrow sup-pression, gastrointestinal reactions and hair loss. And the incidences of the above three toxic reactions were 59.4%, 39.1%, and 81.1% in the endostar combined with chemotherapy group and 64.5%, 38.7%, and 83.9% in the simple chemothotherapy group. The toxic reactions were mainly related to chemotherapeutics and were not significantly different between the two groups (P>0.05). Conclusion: Endostar combined with chemothera-py is a safe method to treat advanced stage NSCLC. Endostar can improve the curative effect of chemothera-py. This method deserves to be clinically practiced and observed further.

To investigate the effects of Tongxinluo capsule on sciatic nerve apoptosis in spontaneous type II diabetic KK/Upj-Ay mice, in order to explore its mechanism for improving diabetic peripheral neuropathy (DPN). KK/Upj-Ay mice were selected as the DPN animal model and randomly divided into the model, Tongxinluo low, middle and high group (1, 2, 4 g x kg(-1)). C57BL/6 mice were selected as the control group. Mice were given intragastrically for 12 weeks. Paw withdrawal latency, motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were detected. Apoptotic rate were detected by FCM. Bcl-2, Bax, Caspase-3 mRNA and protein expression in sciatic nerve were examined by Real-time PCR and Western blot. p38MAPK, p-p38MAPK expression were examined by Western blot. In this study,the authors found that Tongxinluo capsule could increase paw withdrawal latency, MNCV and SNCV. Apoptotic rate of sciatic, the expression of Bax and caspase-3 were lower, while Bcl-2 expression was higher in Tongxinluo group than those in model mice. The expression of p-p38MAPK significantly decreased in Tongxinluo group. The results showed that Tongxinluo capsule has protective effects on diabetic peripheral neuropathy of mice via inhibiting cell apoptosis and suppressing the expression of p-p38MAPK.
Animals ; Apoptosis ; drug effects ; Capsules ; administration & dosage ; Diabetic Neuropathies ; drug therapy ; physiopathology ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Sciatic Nerve ; cytology ; drug effects

Objective To discuss two simulation studies on the closed and open peritoneum in abdomenal operations. Meth-ods We selected 120 domestic female rabbits from animal laboratory of Qi Qi Ha’er Medical College at random and divided them into four groups(according to whether the peritoneum was open or not,the degree of peritoneum defect at the right side of incision and the existence of peritoneum hemorrhagic focus ),with 30 cases in each group. Group Ⅰ: no peritoneum suture and making a defect of 4cm?3cm at the right side of the incision; Group Ⅱ: no peritoneum suture and making a defect of 4cm?3cm at the right side of the incision,with a hemorrhagic focus at peritoneum defect;Group Ⅲ: with peritoneum suture; Group Ⅳ:with dense and compact peritoneum suture.And then we analyzed postoperative peritoneum healing progress. Results Observeations of the incision infection by the naked eye were that one case was identified in group Ⅰ,Ⅲ and Ⅳ;and a significant difference of occurrence rate was identified between the fallowing groups: groupⅠandⅡ(P

Objective To evaluate the effect of driving forces similar to those found in dental plaque fluid on the enamel demineralization and ultrastructure of surface layer.Methods The sections of human enamel were exposed to lactic acid solutions with degree of saturation with respect to enamel and degree of saturation with respect to fluoroapatite for 96 h at 25 ℃,using the deionized water as control in the same conditions.Enamel demineralization was monitored using SEM and confocal laser scanning microscopy(CLSM).Results Only was enamel subsurface demineralization detected in solutions with DS_(EN)(0.1-0.3).Enamel mineral loss and micropores of surface layer decreased significantly with increasing DS_(EN) and DS_(FA) values.However,no mineral loss and micropores of surface layer was observed in sections of enamel exposed to solutions with DS_(EN) values of 0.4 and 0.5,which were covered with homogeneous remineralization layer.Conclusion The demineralization driving forces similar to dental plaque fluid under different DS_(EN) and DS_(FA) have significant effects on the surface layer of enamel and the subsurface demineralization process.

Objective: To establish a new specific and high-throughput method for CYP450 quantification, and to investigate the effect of drugs on rat liver CYP450 using Salvia miltiorrhiza and Panax ginseng as tool drugs. Methods: Mass spectrometry with multiple reaction monitoring (MRM) was applied to quantify nine rat liver enzyme CYP450 isoforms from the rat model with S. miltiorrhiza and P. ginseng. With QconCAT heavy peptides as internal standard, we aimed to assess the effects of S. miltiorrhiza and P. ginseng on CYP450 isoforms. Results: The relative standard deviation and relative error of this method were less than 5.9% and 6.8%, respectively, with good linearity (r2>0.9). Nine CYP450s isoforms (CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP2C6, CYP2C11, CYP3A1, CYP3A2, and CYP17A1) in rat liver microsome treated with S. miltiorrhiza and P. ginseng were also quantified. Compared with the control, S. miltiorrhiza downregulated the expression levels of CYP1A1, CYP2B2, CYP3A2, CYP2C11, and CYP17A1, while upregulated CYP1A2 and CYP2B1. For the effects of P. ginseng, the expression of CYP1A1 and CYP2B2 was decreased, while CYP1A2, CYP2B1, CYP3A2, CYP2C11, and CYP17A1 were increased. Conclusion: We successfully construct a method with MRM to quantify rat liver CYP450 isoforms. And we firstly quantify CYP2B1, CYP2B2, and CYP17A1 in rat liver. The results reveal the regulation of CYP450 by S. miltiorrhiza and P. ginseng, which could provide clinical application for drug compatibility in practice, and avoid adverse drug reactions.

Objective To evaluate the efficacy and safety of open occluded coronary artery branch through outside stent balloon expansion technique.Methods A retrospective analysis of 26 patients,26 interventional treat-ment of coronary bifurcation lesions were taken.All patients initially used conventional interventional techniques, branch reserve protection guidewire and main branch stent.When branch occlusion occured during operation and failed again through guidewire,small outer diameter of the balloon branch guidewire which was squeezed could be used to branch opening.The blocked branch opening was expanded outside of the stent,and the balloon was expanded after the guide wire enter into branch again.The main branch/branches kissing balloon inflation or main branch /branch double stenting would be used when necessary.Results Among 26 cases of bifurcation branch occlusion,25 cases successfully completed the outer stent balloon,achieved a branch balloon expansion after reentry guidewire,saved blocked branches and the rate of success was 96.2%,and 6 cases of the main branch and the branch kissing balloon dilatation,2 cases of remedial double stenting.Following -up for 1 -12(5.3 ±6.8)months after operation in 25 cases of patients,there was no death and myocardial infarction and other adverse cardiovascular events.Conclusion Out-side stent balloon expansion technique can improve the success rate of coronary occlusion branch opening,and it have fewer complications and worth of clinical application becasuse of its satisfactory results.

Objective To investigate the clinical effects of botulinum toxin A ( BTX-A) local injection treatment for hemifacial spasm .Methods Seventeen sites were used to inject BTX-A in 46 patients with hemifacial spasm . Before and after treatment , the grades of facial spasm were evaluated .Clinical efficacy was evaluated based on hemifacial spasm rating 4 weeks after treatment .The recurrence of symptoms in patients given repeated BTX-A injection.Results After BTX-A injection, 15 cases of completely relieved , obviously relieved in 29 cases, partial remission in 2 cases;the efficiency was 95.6%.The onset time was (2.6 ±1.1) d;sustained efficacy was (19.1 ± 2.3)weeks.Injection times was 1 in 5 cases, 2 in 23 cases, 3 in 11 cases and 4 or more in 7 cases.The onset time of repeated injection was(2.61 ±1.03) d, the duration was(19.24 ±2.12) weeks and the efficiency was 100%, They had no significant differences between initial and repeated injection .Six cases occurred local facial mild paralysis, and complete recovery after 2-4 weeks.Conclusion BTX-A local injection for hemifacial spasm is a safe and effective treatment , has no significant adverse reaction;and the effect of repeated injection is not decreased .

Lamellar bodies, tubular and lattice myelin figures (TMF and LMF)of the Type Ⅱ epithelial cells lavaging from the lungs of adult male rats following injection of a single dose of 10mg of active carbon powder, silica dust, uranium ore dust and U_3O_8 powder were observed by scanning and transmisson electron microscope as well as freeze-etching technique. According to the membrane split theory and our observations, we suggested that the lamellar bodies were composed of a lot of subunits, the square crystalls. These crystals linked up each other to form a cuboidal structure and arranged regularly into ring-like lamellae. A central core was surrounded by the lamellae to constitute a lamellar body, which can be distingushed into three types, namely the wollen ball-shaped, the circular concentric shaped and the lattice-shaped. When the circular concentric lamellar bodies were dissociated from the type Ⅱ epithelial cells, they then extended to form the pulmonary surfactant system (LMF). The amount of the pulmonary surfactant system substances in the rats administrated the above-mentioned foreign substances was obviously higher than that of controls.

AIM: To delevop new drug delivery system for volatile oil of lignum dalbergia odoriferae and to improve its effect. METHODS: Solid-lipid-nanopaticals were prepared by hot-dispersiom technique and treated further by sonication; the contents of dispersion and entrapment efficiency of nanoparticles were determined by HPLC. RESULTS: Solid lipid nanoparticles containing volatile oil of lignum dalbergia odoriferae was spherelike; The partical size was 40.0nm; the content of dispersion was 72.1%; entrapment efficiency of nanoparticles was 91.27%. CONCLUSION: Solid-lipid-nanopatical may be a suitable drug delivery system for volatile oil of lignum dalbergia odoriferae.

Objective To assess the efficacy of the laryngeal mask airway i-gel (LMA i-gel) in patients undergoing neuro-interventional surgery. Methods Forty ASA Ⅰ or Ⅱ patients of both sexes, aged 20-60 yr,body mass index ＜ 30 kg/m2, undergoing elective neuro-interventional surgery, were randomized into 2 groups (n = 20 each):LMA i-gel group (group I) and LMA ProSeal group (group P). After induction of anesthesia with TCI of propofol and remifentanil, LMA i-gel and LMA ProSeal were inserted in group I and P respectively. The patients were mechanically ventilated and PETCO2 was maintented at 35-40 mm Hg,SpO2 at 99%-100%. BP and HR were monitored and recorded before induction, immediately after induction, at 1, 3 and 5 min after insertion of LMA and immediately after removal of LMA. The success rate, LMA placement time, leak pressure, peak airway pressure and complications were recorded. Results There was no significant difference in the success rate, the LMA platcement time and peak airway pressure between the two groups (P ＞ 0.05). BP and HR were within the normal range during operation in both groups. The leak pressure and incidence of blood stain and sore throat were significantly lower in group I than in group P (P ＜ 0.05). Conclusion LMA i-gel can provide adequate ventilation during operation with less complications and can be used effectively for neuro-interventional surgery.