Background and objective With development of modern medicine, eliminating patients’ pain and mental disorder and improving the quality of life has become an important problem in patients with cancer. The aim of this study is to observe the impact of chemotherapy on quality of life and influencing factor of quality of life among lung cancer patients. Methods Sixty-one lung cancer patients were assessed with clinical outcomes and the EORTC QLQ-C30 questionnaires before chemotherapy, one week after 2 cycles of chemotherapy, one week after 4 cycles of chemotherapy. Results After 2 cycles of chemotherapy, effective rate was 40.0%. Social function decreased. Nause and vomiting, insomnia and appetite loss deteriorated (P<0.05). After 4 cycles of chemotherapy, effective rate was 23%. But after chemotherapy, physical, role, emotional, social function decreased; Appetite loss, nausea and vomiting, insomnia deteriorated, economics became difficult to some patients (P<0.05). In our study, we find female patients had more fatigue and appetite loss than male patients (P<0.05). Patients of low level of education had more fatigue and insomnia than patients of high level of education (P<0.05). Elder patients had more fatigue, appetite loss, nausea and vomiting than middle-aged patients (P<0.05). Grouped according to body mass index, the difference wasn’t statistically significant (P>0.05). Grouped according to the plasma albumin level, the difference wasn’t statistically significant (P>0.05). In hypoproteinemia group, symptoms and economic difficulties score was higher; High protein group, function and general health scores was higher. Conclusion After the chemotherapy, patients’ the lesion of cancer became smaller and clinical symptoms relieved, but some patients, fatigue, appetite loss, nausea and vomiting symptom deteriorated, emotion became bad. Quality of life of lung cancer patients decrease. We should pay more attention to the adverse reactions of chemotherapy and cope with them, give positive psychological intervention and improve patients, nutrition to improve the quality of life.

italic>Artemisia argyi is a traditional Chinese medicine in China, which is used as medicine with its leaves. The leaves of A. argyi mainly contain flavonoids, phenolic acids, volatile oils and other compounds, and have a variety of pharmacological activities. AP2/ERF transcription factors are abundant in plants and are mainly involved in plant growth and development, abiotic stress response and secondary metabolite biosynthesis regulation. However, there are few reports on the AP2/ERF gene family and its functions in A. argyi. In this study, we systematically identified the AP2/ERF gene family in A. argyi genome, and analyzed its phylogenetic tree, protein physicochemical properties, subcellular localization, conserved motifs, promoter elements, and expression patterns. The results showed that a total of 204 AP2/ERF transcription factors were identified in A. argyi genome, encoding proteins consisting of 88-483 amino acids with a relative molecular mass of 10-52.94 kDa and a theoretical isoelectric point of 4.62-9.88. Subcellular prediction showed that the majority of AP2/ERFs were located in the nucleus, cytoplasm, and the minority of them is located in the membrane and chloroplasts. According to the Arabidopsis AP2/ERF family classification, A. argyi AP2/ERF proteins were divided into four subfamilies: Soloist, AP2, ERF (B3, B4, B5, B6), and DREB (A1, A2, A4, A5, A6), among which DREB family accounted for the largest proportion, and the same subfamily had similar conserved motifs. cis-Acting element analysis showed that AP2/ERF promoters have a large number of elements responding to light and abiotic stress. Expression pattern analysis showed that most of the genes of the AP2/ERF family were dominantly expressed mainly in roots and stems, of which 19 were dominantly expressed in leaves, 77 would be induced to be expressed by methyl jasmonate, of which 16 genes were both dominantly expressed in leaves and induced to be expressed by methyl jasmonate, and these AP2/ERF genes may be the key regulatory genes for the synthesis of active ingredients in A. argyi leaves.This study lays the foundation for the functional study of AP2/ERF family genes and their regulating roles in the active components biosynthesis in A. argyi leaves.