Gestational trophoblastic neoplasia (GTN) includes invasive mole and choriocarcinoma. Before the discovery of effective chemotherapeutic agents for human chorionic gonadotropin (HCG), the survival prognosis for the patients with the disease was very poor. At present, with the setup of sensitive quantitative assays and new drugs to be developed for HCG, most women with GTN can be cured and their reproductive function preserved. The purpose of this paper is to address current opinions regarding the diagnosis, staging, and management of GTN.

For a long time, in the field of medical research, due to lack of proper methods, research decision-making process has not made the most of human experience, recommendations and different opinions, viewpoints, and expositions related to subjective interpretation. At present, this problem in the course of the standardized clinical research on traditional Chinese medicine is particularly prominent and needs to be solved urgently. Consensus methods can be used for studying different perspectives within the group and get different levels of "consent" in order to achieve "consensus", especially in the formation of a consensus of experts, which are widely used now. Consensus methods include three frequently used methods such as Delphi method, nominal group technique and consensus development conference. The research team of WHO Western Pacific Regional Primary Osteoporosis Clinical Practice Guideline of Traditional Medicine completed the preparation of good practice points in the first draft after three expert consensus meetings by using consensus development conference method. This article discusses the application of consensus development conference method in the developing process of clinical practice guidelines of traditional medicine.

It is not finally concluded how to standardize the use of qualitative research in the world. Qualitative researchers disagree with each other about this issue. As we know, there have been a large number of articles written in different ways about qualitative research due to the "flexibility", one of its features. Qualitative research is quite different from quantitative research which is easy to control its quality and quality assessment. A series of criteria has been set up for quantitative research. However qualitative research needs to be improved in these aspects, in which qualitative interviews are mostly used at home and abroad at present. Hence, it becomes an important and urgent issue for qualitative researchers to standardly control and assess the quality of qualitative interview.

Qualitative interview can collect data independently and can help modify and explain data when being combined with the quantitative research. The authors of this article introduced some kinds of qualitative interviews and their application in clinical research of traditional Chinese medicine (TCM). It emphasized some aspects of qualitative interview including the origin and definition, classification, basic standards, sampling, data analysis and report writing, quality evaluation and application for TCM clinical research.

The paper aims to explore basic steps, regulation and data analysis methods of semi-structured and in-depth interview studies of clinical research in traditional Chinese medicine (TCM), and to provide some helpful information obtained from the examples for decision making in TCM research field. A pilot study was made with eight interviewees and, based on which, a formal one with twelve interviewees was done in this study. All the recordings were transcribed into verbal manuscripts, categorized into different archives and labeled by letter from A to T. Grounded theory for analyzing qualitative data was used in this study, and cross-case analysis was also made on the basis of case-by-case analysis results. Nine theories with central categories were constructed by three coding procedures from grounded theory. Three kinds of relationships, three types of optimized attitude, and three concerns were obtained from the example study. Nine analyzing conceptual graphs and nine theories related to optimization of TCM clinical research program were established finally. The researchers of large national studies were included as interviewees in the study. A comprehensive and critical data analysis from twelve interviewees for in-depth interview was conducted in accordance with current international qualitative research, and a response theory model focused on optimization of TCM clinical research program was established. The study laid the foundation for using semi-structured and in-depth interview for TCM clinical research.

Objective To compare color doppler ultrasound examination for the different pathological types of thyroid cancer.Methods 62 cases of thyroid cancer in patients with color doppler ultrasound examination,then compared with pathological examination and compared the different pathological types of thyroid cancer with ultrasound image map features.Results Ultrasound examination showed papillary adenocarcinoma in 31 cases within the hypoechoic,ill-defined in 28 cases,there was calcification in 31 cases,follicular carcinoma in 6 cases、6 cases and 12 cases,there were significant differences between the two groups ( x2 =27.884,20.545,13.964,P =0.000 ),There was no significant difference both in the form of regularity,the nature of the back echo,Vmax and RI.( P =0.427,0.338,0.110,0.872 ).Pathology consistent with papillary carcinoma was 80.0％,follicular carcinoma pathology found was 70.4％.Conclusion Color doppler ultrasound examination of the different pathological types of thyroid cancer had a certain differential diagnosis and it had higher rate of pathological diagnosis.

Objective:To investigate the influential factors of mental health in rural high-school students by structural equation modeling.Methods:447 rural high-school students were tested by Mental Health test,Adolescent Self-rating Life Events Check list,Social Support Scale and Trait Coping Style questionnaire,then theoretical model were established and confirmed for the relationships of life events,social support,coping styles and mental health of rural high-school students.Results:The structural equation modeling fitted the data very well(?2/df=3.56,GFI=0.940,AGFI=0.902,NFI= 0.913,CFI=0.901,RMSEA=0.048),the theoretical model suppose was supported by the use of structural equation modeling.Conclusion:Social supports and coping styles have direct influence on mental health of rural high-school students.Life events have indirect influence on mental health through social supports and coping styles.

BACKGROUND:Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect. OBJECTIVE:To investigate the effect and safety of umbilical cord blood stem cel transplantation in the treatment of diabetic lower limb ischemia. METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points
were selected for injection of human umbilical cord mesenchymal stem cel suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded. RESULTS AND CONCLUSION:At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, al animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, sweling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cel transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility. Cite this article:Xie LH, Xing L, Zheng H. Feasibility of umbilical cord blood stem cel transplantation for the treatment of diabetic lower limb ischemia. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):78-82.

Objective: To investigate the effect of F.nucleatum(Fn) and P. gingivalis(Pg) on soluble intercellular adhesion molecule-1(sICAM-1) in peri-implant sulcular fluid(PISF).Methods: PCR was used to detect microorganism samples from PISF. According to detection of Fn and Pg, 51 titanium implants in 32 partly edentulous patients were divided into 3 groups. Clinical parameters included PD, mPLI and sICAM-1. Periopaper strips were used to collect PISF and an ELISA technique was applied to measure the levels of sICAM-1. Results: PD was significantly greater in the patients with Pg and/or Fn than that in those without Pg or Fn (P