OBJECTIVE:To establish an HPLC method for the determination of reserpine in Reserpine injection.METHODS:The determination was performed on Venusil XBP-C18 column with column temperature at 28 ℃.The mobile phase consisted of methanol-water (52:48,pH value of (3?0.2)) with flow rate of 1 mL?min-1.The detection wavelength was 268 nm and sample size was 10 ?L.RESULTS:The linear range of reserpine was 4～l6 ?g?mL-1 (r=0.999 1) with average recovery of 99.69% (RSD=0.98%,n=9).CONCLUSION:The method is accurate,reliable and reproducible for content determination of Reserpine injection.

BACKGROUND:Polytetrafluoroethylene (PTEE) as a nose filer material has advantages on the resistant to corrosion, chemical stability and so on. However, its linear expansion coefficient is large easily leading to infection and rejection, and its application has some limitations. OBJECTIVE:To compare the cel toxicity, inflammatory infiltrates and biocompatibility indexesin vivo between PTEE and PTEE combined with type Ⅰ colagen. METHODS: MTT method was used to detect the relative proliferation rate of L929 cels cultured with PTEE extract or PTEE combined with type Ⅰ colagen extract; an electron microscope was used to observe the cel growth. PTEE or PTEE combined with type Ⅰ colagen was embedded under the nasal dorsal fascia of New Zealand white rabbits for 7 days, and hematoxylin-eosin staining was used to detect whether nasal mucosa epithelial tissue had inflammatory infiltration. Systemic toxicity, alergies, pyrogen reaction and deaths of rabbits were observed after injection of two kinds of material extracts by ear vein. RESULTS AND CONCLUSION: The cytotoxicity and inflammatory infiltration were milder in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). The alergic reactions and pyrogen reactions were fewer in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). These findings indicate that PTEE combined with type Ⅰ colagen as the nose filer material has better biocompatibility.

Purpose: In order to explore the clinical effect in acupuncture treatment of vascular headache. Methods: 93 cases of vascular headache were randomly auocated into acupuncture group (n= 30), treated by puncturing Fengchi (GB 20), Ashi point and Sanyinjiao (SP 6) on the sick side, acupuncture plus needle-embedding group (n= 36), treated by basic acupuncture treatment plus needle-embedding method in Taiyang (Ex-HN 5) and Xuanzhong (GB 39) on the sick side, and control group (n = 27), treated with oral administration of 5 mg Sibelium,twice a day, for observation of the changes in the clinical symptoms before and after treatments in the patients of three groups. Results: After treatment, the clinical symptoms in the patients of three groups were relieved or disappeared. The recent therapeutic effect was better in acupuncture plus needle-embedding group than in the control group (P＜ 0.05), and there was no significant difference (P＞ 0.05) between the acupuncture plus needle-embedding group and acupuncture group and there was no significant difference (P ＞ 0.05) between acupuncture group and control group, either. The long-term therapeutic effect was better in the acupuncture plus needle-embedding group than in the control group (P＜ 0.01), in the acupuncture group than in the control group (P ＜ 0.05), and there was no significant difference between the acupuncture plus needle-embedding group and acupuncture group. Conclusion: Acupuncture treatment of vascular headache offers a better recent and long-term therapeutic effect, and acupuncture plus needle-embedding method is much better in the therapeutic effect.

Objective To analyze the application of color Doppler ultrasonography in diagnosis of diabetic carotid sclerosis and to study the relation between diabetes and carotid sclerosis. Methods Color Doppler ultrasound findings in 68 hospitalized type 2 diabetes patients ( as an observation group) and in 73 healthy individuals ( as a control group) were compared and analyzed from Feb. 2007 to Feb. 2009. Results The tunica intima and media of carotid were significantly thicker in observation group than in control group ( P

Glucocorticoid is an important treatment for many pediatric diseases and it often leads to osteoporosis, osteonecrosis of the femoral head, and growth retardation. Among them, glucocorticoid-induced growth retardation has been a particular focus in the treatment of children patients, which leads to a decrease in final height. The mechanism of glucocorticoid-induced growth retardation is complex, and there is no safe and effective treatment.This article summarized the mechanisms underlying glucocorticoid-inhibited longitudinal bone growth and reviewed the potential therapeutic targets discovered in recent years.

Concentrations of TSH in the serum of 47 iron-deficiency children and urinary excretion of 17-KS in 24-hour samples from 64 iron deficiency children were investigated before and after treatment, and compared with that of the normal control group. It was showed that serum concentrations of TSH and 17-KS excretion in iron-deficiency children were abnomally higher than in the nomal control subjects, and they were before treatment than after. After treating, they returned to control levels. It is concluded that iron rather than anemia play a role in the disturbance of TSH and corticoid metabolism of the iron-deficiency children.

Bone Marrow Cells ; cytology ; Fibroblasts ; cytology ; metabolism ; Fibrosis ; Humans ; Myocardium ; pathology

Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases

Objective To explore the effect of progesterone on the rate of neuronal apoptosis and nitric oxide(NO) level in the cortex and hippocampus tissue of newborn rats with hypoxic-ischemic encephalopathy(HIE).Methods Thirty 7-day-old neonatal rats were randomly divided into 3 groups:sham-operated group,hypoxic-ischemic(HI) group and pretreatment group.Rats in HI group and pretreatment group were subjected to left common carotid artery ligation,then were exposed to 80 mL/L oxygen and 920 mL/L nitrogen gas in 37 ℃ closed container for up to 2.5 h to establish HIE model.Progesterone was injected intraperitoneally into rats in the pretreatment group respectively for 30 minutes before hypoxia,and solution was injected into the sham-operated group and HI group.All rats were killed at 24 h after operation.The neuron apoptosis was identified and analyzed by flow cytometry.Nitrate/nitrite was assayed to represent nitric oxide content of brain tissues.Results The ratio of neuronal apoptosis and NO contents in cortex and hippocampus tissue in HI group [(10.09?0.36)%,(12.32?0.28)%,(51.36?9.71) ?mol/L,(52.34?4.26) ?mol/L] were significantly higher than those in sham-operated group [(2.49?0.23)%,(2.58?0.26)%,(18.16?6.24) ?mol/L,(19.28?3.58) ?mol/L)](P_a

Objective To investigate the clinical effect of salmon calcitonin in the treatment of senile osteoporosis , and to observe the effect of salmon calcitonin on bone mineral density ( BMD ) and pain.Methods 180 cases of patients diagnosed as osteoporosis from September 2015 to September 2016 in our hospital were retrospectively analyzed, the patients were divided into control group and treatment group according to the random number table method.The clinical efficacy and side effects of the two groups were evaluated.The bone mineral density (BMD) of the lumbar vertebrae, femur and radius were measured by dual energy X-ray absorptiometry.The VAS was used to evaluate the two groups before and after the treatment.( ODI) were used to evaluate the functional status of the patients before and after treatment.The self-made questionnaire was used to evaluate the quality of life of the patients before and after treatment.Results The total effective rate of treatment group was 94.44%, significantly higher than the control group 75.56%(P<0.05).After treatment, the treatment group of lumbar spine bone mineral density, bone mineral density and radial bone mineral density was significantly higher than the control group (P<0.05).After treatment, spontaneous low back pain, flexion extension pain, turn over pain and weight pain were significantly lower in the treatment group than in the control group (P<0.05).The ODI index of the treatment group was significantly lower than that of the control group (P<0.05).After treatment, the quality of life scores in the two groups were significantly higher than those before treatment, and the treatment group was higher than the control group(P<0.05).No significant adverse reactions occurred in two groups during the treatment.Conclusion Salmon calcitonin treatment of osteoporosis in elderly patients with a significant effect, help to improve bone mineral density, reduce pain and improve its dysfunction, and has high security.