The expression of Toll-like receptor 4 (TLR4) in neonatal cord blood mononuclear cells (MNCs) and serum TNF-α were investigated in order to explore the roles of TLR4 in the pathogenesis of preeclampsia. The study enrolled 27 patients suffering from preeclampsia (experimental group) and 21 normal pregnancy patients (control group). After MNCs were separated, the expression of TLR4 mRNA and protein was detected by using real-time quantitative PCR and Western blotting respectively, and the expression of TNF-α by using ELISA. The results showed the TLR4 mRNA level in cord blood MNCs (2(-ΔCT): 0.07±0.17), TLR4 protein expression level (absorbance ratio: 0.81%±0.15%) and TNF-α level (9.5±1.73 pg/mL) were all increased in experimental group as compared with control group with the differences being statistically significant (P<0.05). There was a positive correlation between the expression of TLR4 mRNA and TNF-± in both experimental group and control group (r=0.54 and 0.53, respectively, P<0.05). It was concluded that TLR4 expression in the experimental group of cord blood MNCs was increased and there was a positive correlation between the expression of TLR4 mRNA and TNF-α in both groups. TLR4-mediated release of inflammatory cytokines may be one of the important reasons leading to preeclampsia.

The expression of Toll-like receptor 4 (TLR4) in neonatal cord blood mononuclear cells (MNCs) and serum TNF-α were investigated in order to explore the roles of TLR4 in the pathogenesis ofpreeclampsia. The study enrolled 27 patients suffering from preeclampsia (experimental group)and 21 normal pregnancy patients (control group). After MNCs were separated, the expression of TLR4 mRNA and protein was detected by using real-time quantitative PCR and Western blotting respectively, and the expression of TNF-α by using ELISA. The results showed the TLR4 mRNA level in cord blood MNCs (2-△CT: 0.07±0.17), TLR4 protein expression level (absorbance ratio:0.81%±0.15%) and TNF-a level (9.5±1.73 pg/mL) were all increased in experimental group as compared with control group with the differences being statistically significant (P＜0.05). There was a positive correlation between the expression of TLR4 mRNA and TNF-α in both experimental group and control group (r=0.54 and 0.53, respectively, P＜0.05). It was concluded that TLR4 expression in the experimental group of cord blood MNCs was increased and there was a positive correlation between the expression of TLR4 mRNA and TNF-α in both groups. TLR4-mediated release of inflammatory cytokines may be one of the important reasons leading to preeclampsia.

Objective To explore the practical effects of multiple intelligence theory applied in Obstetrics and Gynecology clinical internship teaching for the medical students.Methods The Eight-year program medical students in the classes of 2009 and 2010 were collected,among which 45 students in theexperimental group were taught by multiple intelligence theory,and 40 students in the control group were taught by traditional teaching methods.The results determined by the questionnaire survey,written test scores,operation test scores,medical documents writing results,usual performance and teachers' job evaluation.The data were analyzed by SPSS 17.0 statistical software,chi-square test and t test.Results Compared with control group,the written test scores,operation test scores,medical documents writing results,usual performance,the student questionnaire and teachers' self work evaluation of the experimental group were significantly higher (89.720 ± 8.147) vs.(81.140 ± 9.142),(88.230 ± 4.328) vs.(80.360 ± 5.621),(86.530 ± 2.641) vs.(81.580 ± 2.036),(87.650 ± 3.957) vs.(82.360 ± 6.392),which showed statistically significant difference (P＜0.05).The experimental group students think the teaching methods with multiple intelligence theory are novel,producing following positive effects in fully inspiring learning initiative,fostering the ability of independent study,enlightening clinical thinking,strengthening collective cooperation and improving the ability of doctor-patient communication and problem-solving etc.Conclusions It shows that the application of multiple intelligences theory in Obstetrics and Gynecology clinical internship for the medical students could motivate the students' self-confidence and enthusiasm,and contribute to train high quality medical talent,Meanwhile,the higher requirements are needed for teachers.It is worth promoting the application.