Objective To develop a secure network system to monitor the temperatures in baby incubators.Methods By utilizing patented TAV temperature monitoring system,the running status of temperature-controlled equipments was recorded in digital way,and then it was analyzed and managed dynamically;and real time alarms could be given through networks.Results This system is a kind of security devices independent of baby incubators and can monitor temperatures in baby incubators well.Conclusion This system will not affect original functions of baby incubators,instead it can well monitor the temperatures in both imported and domestic incubators,so that it can improve the safety of facilities being used while decrease medical accidents.

Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.

Objective:To investigate the effect of telmisartan and fosinopril on the expression of angiotensin-convertion enzyms 2 (ACE2) in cardiomyocyte after myocardial infarction.Methods:Animal model of acute myocardial infarction were established by ligating the left anterior descending coronary artery of rats. Vehicle, telmisartan,fosinopril,or both drugs combined were giv- en to rats by intubation feeding,from 2 weeks before coronary artery ligation to 2 weeks there after. ACE2 protein expression of myocardium were semi-quantified by SP immunohistochemistry. ACE2mRNA were measured by Reverse Transcriptase Poly- merase Chain Reaction (RT-RCR). Results:Compare with sham operated group,ACE2 protein increased significantly in infarc- tion group,but not the ACE2 mRNA. telmisartan and fosinopril therapy caused a significant increase in ACE2 protein compare to infarction group,but only telmisartan caused a significant increase in ACE2 mRNA though all therapied groups showed higher ACE2 mRNA expression. Conclusion:Both telmisartan and fosinopril induced increases in cardiac ACE2 exprssion, whereas the combination of these two drugs do not associated with higher ACE2 expression.

The PTC hot air drying device controlled by a single-chip computer has such advantages as rapid heating, automatic isothermal, weak leakage current and being time-proof, which applies to all calibers of pipeline.

Objective To balance and optimize four key control parameters, acid-base pH, viral spiking(MOI), TPCK trypsin spiking and cell density in MDCK cell suspension culture of influenza virus H3N2, in order to improve the productivity and virus titer of influenza virus H3N2 in MDCK cell suspension culture.Methods A multi-factorial interaction experimental design was created using MODDE~?software of Design of Experiment(DoE) from Sartorius to determine and optimize the infectivity titer of H3N2 virus, considering the interactive effects of four factors: pH, MOI, TPCK trypsin concentration and cell density, aiming to achieve an optimal infectivity titer within a certain range of these four factors.Results The optimal ranges of MDCK cell suspension culture conditions for H3N2 virus were as follows: pH range of 7. 88-8. 00, MOI of 0. 001,TPCK trypsin concentration range of 1. 70 to 6. 0 μg/mL, and cell density range of(55. 70-77. 28 × 10~5) cells/mL. The virus was maintained in Xene-S001 medium at a cultivation temperature of 34. 5 ℃ for 72 h, with a shaking speed of 100 r/min and a CO_2 concentration of 3%. Under these conditions, the TCID_(50) of H3N2 reached 6. 5, and the CV of repeated experiments for TCID_(50) was 1. 03%, with good reproducibility and stability.Conclusion H3N2 virus in MDCK cell suspension culture can achieve higher virus titer.

Several researches have suggested that estrogen contributes to the initiation and development of thyroid cancer by binding to estrogen receptors (estrogen receptor α,estrogen receptor β,and G-protein-coupled receptor 30),activating gene or non-genomic pathways and regulating proliferation of thyroid cells.Studies on antiestrogen drugs based on inhibiting thyroid cell proliferation may provide a new target in treating thyroid cancer.

Child ; Child, Preschool ; Epilepsy, Generalized ; complications ; epidemiology ; genetics ; Female ; Humans ; Infant ; Male ; Pedigree ; Phenotype ; Seizures, Febrile ; complications ; epidemiology ; genetics

Objective To investigate the effects of aminolevulinic acid-based photodynamic therapy(ALA-PDT) on chicken combs, an animal model for port wine stains (PWS), and to explore the feasibility of PWS treatment with ALA-PDT. Methods A total of 80 leghorns were randomly and equally divided into 10 groups: blank control group receiving no treatment, ALA group treated with ALA alone, four single laser groups irradiated with 630-nm red laser at 75, 100, 150 and 200 J/cm2 respectively, four ALA-PDT groups pretreated with ALA followed by 630-nm red laser radiation at 75, 100, 150 and 200 J/cm2 respectively. An area sized 1 cm × 1 cm were marked at one side of combs in all these leghorns, and served as the experiment area to receive corresponding treatment, with that in the other side as the control area. Tissue specimens were obtained on the 14th and 28th days after treatment followed by the observation of morphological and histological changes, calculation of decrement rate in capillary number, and determination of apoptosis index in vascular endothelial cells (VECs) in chicken combs. Results In all the four ALA-PDT groups, the combs became lighter in color with apoptosis of some VECs as well as a decrease in capillary count and diameter in the dermis of the experiment areas. The decrement rate in capillary number was 33.53% ± 4.89%, 52.02% ± 2.77%, 67.48% ± 5.58%and 88.96% ± 2.47% respectively, and apoptosis index in VECs was 63.44 ± 1.09, 88.50 ± 6.11, 94.32 ± 3.67 and 113.76 ± 10.57 respectively, in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day after treatment, and both the decrement rate and apoptosis index in each of these groups were significantly different from those in the blank control group, ALA group, single laser groups receiving red laser radiation at the corresponding dose, and the other ALA-PDT groups (all P < 0.01)separately. The apoptosis depth of VECs, defined as the vertical distance from the basal layer to the deepest level at which VEC apoptosis occurred, was 201.19 ± 0.33 μm, 266.15 ± 1.02 μm, 546.09 ± 2.45 μm and 766.37 ± 1.08 μm respectively in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day, with significant differences between these four groups (all P < 0.01). Conclusions ALA-PDT can markedly damage capillaries in the animal model of port wine stains, chicken combs, with the degree and depth of capillary damage associated with red light energy density. The induction of VEC apoptosis may be an action mechanism of ALA-PDT in the treatment of PWS.

ObjectiveTo observe the clinical efficacy of acupuncture plus psychological intervention in treating postpartum depression and its effect on 5-hydroxy tryptamine (5-HT), orphanin FQ (OFQ) and estrodiol (E2).MethodTotally 158patients with postpartum depression were randomized into an acupuncture group, a psychological intervention group, and a treatment group, 52 cases in the acupuncture group, and 53 cases respectively in the psychological intervention group and treatment group. The acupuncture group was given acupuncture, the psychological intervention group was given psychological intervention, while the treatment group was given acupuncture plus psychological intervention. After 6-week treatment, the clinical efficacies, the Hamilton Depression Scale (HAMD) score, Self-rating Depression Scale (SDS) score, Self-rating Anxiety Scale (SAS) score, 5-HT, OFQ, and E2were observed.ResultThe total effective rate was 92.5% in the treatment group, versus 79.2% in the psychological intervention group and 76.9% in the acupuncture group. TheRiditanalysis showed that the therapeutic efficacy of the treatment group was significantly superior to that of the psychological intervention group and acupuncture group (P<0.05), while there was no significant difference between the acupuncture group and psychological intervention group (P>0.05). After treatment, the HAMD score, SAS score, SDS score, 5-HT, OFQ, and E2levels were significantly different from those in the acupuncture group (P<0.01); after treatment, the HAMD score, SAS score, SDS score, 5-HT, OFQ, and E2levels of the treatment group were significantly different from those in the psychological intervention group (P<0.01); after treatment, the HAMD score, 5-HT, OFQ, and E2levelsof the psychological intervention group were insignificantly different from those of the acupuncture group (P>0.05), while there were significant differences in comparing the SAS and SDS scores between the two groups (P<0.01).ConclusionAcupuncture plus psychological intervention can markedly enhance the clinical efficacy in treating postpartum depression, improving HAMD score, decreasing SAS and SDS scores, and regulating the 5-HT, OFQ, and E2levels.

Objective To investigate the effect and mechanism of Src kinase in renal interstitial fibrosis of unilateral ureteral obstruction (UUO) mice.Methods Male C57BL/6J mice were randomly divided into 4 groups,including sham operation group (n=8),sham operation+PP2 group (n=8),UUO operation group (n=8) and UUO operation+PP2 group (n=8).The mice were injected 2 mg/kg PP2 by intraperitoneal everyday after surgery in sham+PP2 group and UUO+PP2 group.PP2 dissolved in 1％ DMSO (formulated with normal saline).Sham and UUO group were given equal 1％ DMSO.The mice were sacrificed at 7th day.Renal collagen was observed with Sirius red stain.The activities of Src,protein kinase B (PKB,AKT),p38 mitogen-activated protein kinase (p38 MAPK),extracellular signal-regulated kinase (ERK) and the protein expressions of α-smooth muscle actin (α-SMA) and fibronectin (FN) were detected by Western blotting.The expression of collagen I (COL [) was detected by immunohistochemistry and the expressions of matrix metalloprotein 9 (MMP-9),tissue inhibitor of metalloproteinase 1 (TIMP-1),transforming growth factor-β31 (TGF-β31),monocyte chemotactic protein-1(MCP-1),interleukin-6 (IL-6) were measured by ELISA.Resuts Compared with sham mice,UUO mice on 7th day displayed obvious renal fibrosis.Meanwhile,UUO mice had increased expressions of COL Ⅰ and FN,and activities of AKT,ERK and p38 MAPK (all P ＜ 0.05).Their renal expressions of α-SMA,TGF-β1,MMP-9,TIMP-1,MCP-1 and IL-6 were also raised (all P ＜ 0.05).Compared with those in UUO group,in UUO + PP2 group the activities of Src,AKT,p38 MAPK and ERK,and expressions of TGF-β1,MCP-1 and IL-6 decreased (all P ＜ 0.05).Additionally,expressions of COL Ⅰ,FN and α-SMA,collagen deposition and renal fibrosis receded in UUO + PP2 group (all P ＜ 0.05).However,the expressions of MMP-9 and TIMP-1 were not influenced by PP2 treatment.Conclusions Src kinase promotes myofibroblasts accumulation and inflammatory reaction through activating its downstream signaling pathway in the progressing of renal interstitial fibrosis.