Animals ; Erythrocytes ; physiology ; Oncorhynchus mykiss ; physiology ; Oxygen ; Water ; chemistry

Objective To evaluate the cleanliness of frequently touched object surfaces in a hospital and efficacy of intervention measures.Methods Compliance to cleaning of frequently touched object surfaces before and after intervention was surveyed by fluorescence labeling method,SPSS 17.0 statistical software was used to analyze data.Results Before and after intervention,6 800 items in 400 wards were investigated,compliance rates to cleaning of hospital object surfaces before and after intervention were 14.71％ and 54.76％ respectively(P＜0.001);differences in compliance rates to cleaning of object surfaces in common wards and special wards before and after interven tion were both statistically significant(both P＜0.001);after intervention,compliance rates to cleaning of object surfaces in wards and toilets increased significantly compared with before intervention,which increased by 41.57％ and 33.00 ％ respectively,differences were statistically significant (both P＜0.001);after intervention,compliance rates to cleaning of different object surfaces increased by 21.50％-52.00％ (all P＜0.001).Conclusion Scientific and effective intervention measures can improve the cleaning effectiveness of frequently touched object surfaces,which can improve the environmental quality of hospital.

The treatment of limb fractures with closed reduction and minimally invasive fixation with C-arm or conventional X-ray guidance has been widely accepted and gradually developed due to less trauma and rapid healing.However,the radiation damage of X-ray can not be ignored.With the continuous progresses of ultrasound technology,the application of ultrasound in clinic is involved not only in the diagnosis of fractures,but also in the closed reduction and fixation of fractures.The research progresses of ultrasound-guided closed reduction and minimally invasive fixation of limb fractures were reviewed in this article.

OBJECTIVE:To systematically evaluate therapeutic efficacy of thalidomide in the treatment of ankylosing spondylitis,and to provide evidence-based reference for clinic.METHODS:Retrieved from Chinese Journal Full-text Database,China Science and Technology Journal Database,China Biology Medicine disc,Wanfang database,Medline,PubMed,Elsevier database and Cochrane library,the references of the included literatures were also reviewed,randomized controlled trials (RCTs) about thalidomide alone or combined with other routine drug (trial group) versus routine treatment or other drug (control group) in the treatment of ankylosing spondylitis were collected.After data extraction,quality evaluation of included studies by modified Jadad scale,Meta-analysis of response rate,bath ankylosing spondylitis disease activity index (BASDAI) score,erythrocyte sedimentation rate (ESR),C-reaction protein (CRP) level,thoracic mobility,pillow wall distance,Schober trial result,morning stiffness time and the number of peripheral joints with swelling and pain were conducted by using Rev Man 5.1 statistical software.RESULTS:A total of 10 RCTs were included,involving 636 patients.Results of Meta-analysis showed that response rate [OR=2.50,95％CI(1.36,4.62),P=0.003],thoracic mobility [MD=0.22,95％ CI (0.01,0.42),P=0.04],occipital wall distance [MD=-0.82,95％ CI (-1.32,-0.32),P=0.001],Schober trial result [MD=0.64,95％CI(0.28,0.99),P=0.000 4] and morning stiffness time [MD=-2.33,95 ％ CI (-3.92,-0.73),P=0.004] of trial group were significantly better than those control group,with statistical significance.There were no statistical significance in BASDAI score [MD=-4.40,95％CI(-8.96,0.16),P=0.06],ESR [MD=-3.51,95％CI(-7.76,0.74),P=0.11],CRP level [MD=-1.60,95％CI(-3.22,0.03),P=0.05] or the number of peripheral joints with swelling and pain [MD=-0.06,95％CI(-0.29,0.17),P=0.60] between 2 groups.CONCLUSIONS:Thalidomide shows significant improve effect on ankylosing spondylitis.Thalidomide is more effective than conventional drugs in the improvement of peripheral joint function and the control of inflammatory indicators.

Objective:To study the characteristics and clinical significance of mitochondrial injury of T lymphocyte subsets in patients with autoimmune hepatitis (AIH).Methods:The clinical data of 57 patients with AIH (AIH group) from June to December 2021 in Hangzhou Xixi Hospital were retrospectively analyzed, while 60 healthy physical examiners were included as healthy group. The peripheral blood T lymphocyte subsets (CD 8+ T lymphocyte count and CD 4+ T lymphocyte count) were detected by flow cytometry, and matched mitochondrial staining value according to certain algorithm was used to determine the mitochondrial damage of helper T lymphocyte (Th cell) and suppressor T lymphocyte (Ts cell). The levels of IgG, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using a Roche E170 automatic electrochemiluminescence immunoassay. Anti-nuclear antibody (ANA) titer was measured by immunofluorescence. Multivariate Logistic regression was used to analyze the independent risk factors of mitochondrial damage of Th cell and Ts cell in patients with AIH. Results:The ALT, AST, IgG, positive rate of ANA titer, CD 4+ T lymphocyte count, CD 8+ T lymphocyte count, rate of Th cell mitochondrial injury and rate of Ts cell mitochondrial injury in AIH group were significantly higher than those in healthy group: (118.90 ± 37.61) U/L vs. (30.96 ± 14.37) U/L, (102.40 ± 36.51) U/L vs. (31.12 ± 14.06) U/L, (18.40 ± 3.71) g/L vs. (13.89 ± 1.98) g/L, 96.49% (55/57) vs. 16.67% (10/60), 438 (323, 637) × 10 6/L vs. 398 (272, 469) × 10 6/L, 296 (211, 296) × 10 6/L vs. 270 (193, 322) × 10 6/L, 61.40% (35/57) vs. 8.33% (5/60) and 82.46% (47/57) vs. 11.67% (7/60), and there were statistical differences ( P<0.01 or <0.05). Multivariate Logistic regression analysis result showed that the AST elevated and CD 8+ T lymphocyte count reduced were the independent risk factors of Ts cell mitochondrial injury in patients with AIH ( OR = 1.06 and 0.99, 95% CI 1.01 to 1.10 and 0.99 to 1.00, P<0.05); the ALT elevated and IgG elevated were the independent risk factors of Th cell mitochondrial injury in patients with AIH ( OR = 1.08 and 1.66, 95% CI 1.02 to 1.14 and 1.11 to 2.48, P<0.05). Conclusions:It is of positive clinical significance to measure the T lymphocyte subtype mitochondrial injury in patients with AIH. The probability of mitochondrial injury of T lymphocyte subtype can be predicted by biochemical indexes such as ALT, AST and IgG, so as to indirectly evaluate the liver cell necrosis.

Objective To evaluate the safety and accuracy of modified percutaneous retrograde intramedullary screwing into the superior pubic ramus or anterior acetabular column for pelvic and acetabular injury.Methods A retrospective analysis was conducted of the 23 patients with pelvic and acetabular injury who had been treated with modified percutaneous retrograde intramedullary screwing into the superior pubis ramus or anterior acetabular column from June 2015 to June 2017 in Luoyang Orthopaedic Hospital of Henan Province.They were 12 men and 11 women,aged from 22 to 88 years (mean,53 years).The injury included 17 pelvic fractures,4 acetabular fractures,and 2 acetabular plus pelvic fractures.The safety and accuracy of screwing were assessed by postoperative physical and imaging examinations.The number of fluoroscopy and operative time were documented for each retrograde intramedullary screwing into the pubic symphysis or acetabular anterior column.The quality of fracture reduction was evaluated by the Mata criteria postoperatively.The Majeed and the Harris scores were used to evaluate the functional recovery of the pelvis and acetabulum at the last follow-ups.Results A total of 31 intramedullary screws were placed in the 23 patients.For insertion of one screw,the operative time ranged from 15 to 50 min (average,35 min) and the number of fluoroseopy from 32 to 55 times (average,45 times).Postoperative physical examinations revealed no iatrogenic neurovascular lesion and uneventful healing of incisions in all the patients.Postoperative imaging examinations showed that 29 screws were located completely in the bone and 2 ones protruded the cortical bone of the pubic anterior border;all the screws did not protrude into the acetabulum or broke through the pubic symphysis or the pubic cortex.By the Matta criteria,the postoperative fracture reduction was rated as excellent in 17 cases,good in 5 and fair in one,giving an excellent to good rate of 95.7％.Of this series,19 were followed up for 6 to 24 months (mean,13 months).All the fractures healed after 3 months.The Majeed scores at the last follow-up for the 13 patients with pelvic fracture were excellent in 12 and good in one.The Harris scores at the last follow-up for the 6 patients with acetabular fracture (including the 2 with pelvic fracture) were excellent in 5 and fair in one.Conclusion The modified percutaneous retrograde intramedullary screwing into the superior pubic ramus or anterior acetabular column is safe,convenient and precise,effectiyely reducing radiation and operative time.

Objective To introduce an efficient way to orientate S1 iliosacral screw guide-pin verified by CT.Methods The pelvic axial CT data in DICOM format of 180 adults between July 2017 and June 2018 were retrieved from the database of Imaging Center,Luoyang Orthopaedic Hospital of Henan Province.The data were input into the software Mimics 20.0 to display the axial,coronal and sagittal sectional views of the pelvis.On the axial CT sectional view displaying the largest osseous pathway in the S1 segment,a virtual iliosacral screw and its virtual guide-pin were accurately placed into the sacral body in an oblique fashion.In the design of ideal insertion,the virtual screw and guide-pin were truly in the pelvic transverse plane when they were located exactly in the pelvic axial CT sectional view,and they intersected the outer iliac table at the start-point which restricted the guide-pin's location,and angulated with the pelvic coronal plane (represented by a line connecting the most dorsal points of bilateral ilia) at an angle (α) which limited the guide-pin's orientation.After three-dimensional pelvic models of the standard lateral sacral view and the pelvic outlet and inlet views in each patient were calculated,they were displayed in a transparent manner using the software,followed by the virtual insertion of the screw and guide-pin.After the start-point was established on the standard lateral sacral view,the guide-pin was orientated into the pelvic transverse plane and at the guide-pin's α angle relative to the pelvic coronal plane,and subsequently inserted into the ilium shallowly for stabilization.The pelvic outlet and inlet views were taken to judge the guide-pin's orientation.If fine orientation was verified,the guide-pin was advanced to its final position,followed by virtual insertion of an iliosacral screw over the guide-pin.After the virtual insertion was completed,the axial,coronal and sagittal CT sectional views of the pelvis were scrutinized to evaluate the accuracy of insertion.Results After all the guide-pins were orientated in the 180 adults (360 sides) on the true sacral lateral view according to the above way,their orientations on the pelvic outlet and inlet views were 100％ fine,leaving further adjustment unnecessary.The intraosseous insertions of the virtual screws and guide-pins were 100％ accurate and safe on the CT sectional images.Conclusion The way introduced here can theoretically guarantee accurate orientations of the guide-pin on the pelvic outlet and inlet views with no more complex guide-pin adjustments,assuring insertion accuracy and enhancing surgical efficiency.

Objective:To discuss the displacement characteristics of Garden type III femoral neck fracture and investigate the reliability, validity and clinical value of the frontal Garden index in assessing the displacement degree of Garden type III femoral neck fracture.Methods:The pelvis X-ray films of 98 patients with Garden type III femoral neck fracture treated at Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital) from October 2010 to October 2018 were collected, including 47 males and 51 females; aged 19-89 years [(64.9±16.2) years]. Three-dimensional data of the hip with 64-slice CT were available in 21 patients. Each patient′s frontal Garden index was measured three times by three senior doctors, which was repeated twice in turn. The distribution characteristics of the frontal Garden index were statistically described. The reliability of the frontal Garden index was tested by Spearman correlation coefficient and Kappa coefficient, including test-retest reliability and intra-rater consistency. The contact area of fracture ends and upper-shift distance of the femoral neck were calculated based on three-dimensional CT data of the hip in 21 patients. Correlation analysis of the contact area of fracture ends and upper-shift distance of the femoral neck with the frontal Garden index was performed by multiple correlation analysis to assess the validity of the frontal Garden index.Results:The Frontal Garden index of 98 patients with Garden type III femoral neck fracture was (136±15) °, with the minimum value of 90 ° and maximum value of 159 °, and was found to be normally distributed ( P>0.05). Spearman correlation coefficient of the test-retest reliability was 0.93, 0.97 and 0.95, respectively (all P<0.01). Kappa coefficient of the intra-rater consistency was 0.87, 0.91 and 0.86, respectively (all P<0.01). The frontal Garden index was positively correlated with the contact area of fracture ends ( r=0.80, P<0.01), and was negatively with the upper-shift distance of the femoral neck ( r=-0.77, P<0.01). Conclusions:The displacement degree of Garden type III femoral neck fracture shows diversity and normal distribution. The frontal Garden index can credibly and effectively measure the displacement degree of Garden type III femoral neck fracture, which may help to choose the treatment plan.

Objective: To evaluate the clinical application effects of an iliac-crest-preserving iliac wing bone graft harvesting technique and the bone regenerative ability of the donor site after harvesting. Methods: From January 2016 to June 2017, a series of 39 patients including 28 men and 11 women aged between 16 and 59 y (35±13 y in average) were prospectively collected to treat bone defects using the iliac-crest-preserving iliac wing bone graft harvesting technique. The patient cohort included 38 ipsilateral and 1 bilateral bone harvests taken from 15 femoral fracture non-unions, 8 tibial fracture non-unions, 11 femoral head necroses (hip preserving surgery), 1 tuberculosis deriving hip arthritis with bone defect (total hip arthroplasty), 2 hip prosthesis loosenings (revision), and 2 proximal femoral benign tumors. All patients were treated through comprehensive surgeries containing autologous iliac bone grafting. The surgical time, blood loss, bone graft volume, 24 h post-operative visual analogue scale (VSA) at the iliac surgical site, complications, and bone regeneration of the donor site were documented and evaluated. Results: In the 39 patients (40 sides), the average surgical time was 25±4 min, average blood loss was 79±23 ml, average bone graft volume was 27±6 cm³. The average 24 h post-operative VAS at the iliac surgical site was 1.8±0.7 points. The VSA at the 6 week later and thereafter were 0 in all patients. The iliac incisions in 38 patients (39 sides) were healed uneventfully. However, seroma at the iliac surgical site emerged in 1 patient at the 6th post-operative day in the manner of serous exudation and was treated successfully with non-operative measures. None infections and lateral femoral cutaneous nerve injuries took place. Iatrogenic non-displaced iliac crest fractures happened in the very first 2 patients when taking the crest as a pivot to pry up the graft, which united without special cure at the 3 month post-operative. This complication was completely avoided when shifting the pivot to the anterior pillar containing the anterior iliac spines. None pelvic fractures and heterotopic ossifications took place. Post-operative radiographical examinations revealed that the bone defects at the donor site shrank through bone regeneration, that mamillary or canine-tooth-shaped bone formation occurred in some of the cases, and that none complete bone regeneration took place to eliminate the bone defect at the donor. There were 12 patients in whom pre- and post-operative computed tomographic scans necessitated by the disease were prescribed, which facilitated the measuring of the bone defect at the donor site. The measurement of 13 sides revealed that the bone defects were decreased more or less: the average immediate post-operative bone defect was 25.7±6.5 cm², the average 12 month post-operative bone defect was 12.7±5.3 cm². Conclusion The iliac wing bone graft harvesting technique suggested here is safe and less invasive, in the premise of preserving the iliac crest and retaining the figure of the surgical site, it can harvest a large amount of iliac wing bone graft and reserve the bone regenerative ability of the donor site