Objective To confirm the localization and function mechanisms of TGF?s and their receptors in mouse spermatogenesis,by observing the expression of TGF?s and their receptors in mouse testicular tissues. Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used. Results Immunohistochemical staining display:Both TGF?1 and TGF?2 were expressed in acrosome of Ⅵ-Ⅷ stages round spermatids and elongated spermatids in seminiferous tubules,in cytoplasm of Leydig cell their expression was very weak;TGF?3 was only expressed in the cytoplasm of Leydig cells and there was no positive expression in seminiferous epithelium;TGF?-RⅠ was expressed in late spermacytes(Ⅷ stage) and early elongating spermatids(Ⅹ stage);TGF?-RⅡ was expressed from the Ⅺ stage elongating spermatids to elongated spermatids released;TGF?-RⅢ was expressed in acrosome of late round spermatids (Ⅶ-Ⅷ stages)and elongated spermatids.Immunofluorescence histochemical double-staining display:the earlist co-expression of TGF?1,TGF?2 and TGF?-R3 in seminiferous epithelium was at acrosome of Ⅵ-Ⅷ stages round spermatids.Conclusion TGF?s and their receptors are expressed with cell-and stage-specifity in mouse spermatogenesis,which suggests that they have an important role in spermatogenesis.