1.Inhibitory effect of xipayi mouth rinse on the secretion of IL-6 from human gingival fibroblast induced by lipopolysaccharide.
Wufuer HAMULATI ; Yusufu AIBIBAI ; Liang-jun ZHONG
Journal of Central South University(Medical Sciences) 2006;31(3):326-335
OBJECTIVE:
To observe the effects of xipayi mouth rinse of different concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by lipopolysaccharide (LPS).
METHODS:
HGF was stimulated with LPS at 25 g/mL, and the radioimmunoassay (RIA) was used to examine the effect of xipayi mouth rinse at 12.5 approximately 200 g/mL on the secretion of IL-6 in the supernatant of the cell culture.
RESULTS:
IL-6 secreted by human gingival fibroblast was significantly inhibited by xipayi mouth rinse in a dose dependent manner.
CONCLUSION
Xipayi mouth rinse can inhibit the secretion of IL-6 from HGF induced by LPS, suggesting the anti-inflammatory effect of xipayi mouth rinse to treat and prevent periodontal diseases.
Anti-Inflammatory Agents
;
pharmacology
;
Cells, Cultured
;
Depression, Chemical
;
Drugs, Chinese Herbal
;
pharmacology
;
Fibroblasts
;
metabolism
;
Gingiva
;
cytology
;
Humans
;
Interleukin-6
;
metabolism
;
Lipopolysaccharides
;
Mouthwashes
2.Effects of xipayi mouth rinse on the DNA synthesis and change of cell cycles of human gingival fibroblast induced by lipopolysaccharide.
Yusufu AIBIBAI ; Wufuer HAMULATI ; Liang-jun ZHONG
Journal of Central South University(Medical Sciences) 2006;31(4):483-486
OBJECTIVE:
To observe the effects of xipayi mouth rinse on the DNA synthesis and change of cell cycles of human gingival fibroblast (HGF) induced by lipopolysaccharide(LPS).
METHODS:
HGF was stimulated with LPS at 25 mg/L. Flow cytometry was used to examine the effect of xipayi mouth rinse at 25 mg/L on the DNA synthesis and change of HGF cell cycles.
RESULTS:
The percentage of HGF in G( 1) phase increased after the cells were induced by LPS, while the percentage of HGF in S phase decreased. Xipayi mouth rinse could ameliorate this phenomenon.
CONCLUSION
Xipayi mouth rinse can significantly ameliorate the inhibitory effect of LPS on the proliferation of HGF, suggesting the anti-inflammatory effect of xipayi mouth rinse in the treatment and prevention of periodontal diseases.
Cell Cycle
;
drug effects
;
DNA
;
biosynthesis
;
Drugs, Chinese Herbal
;
pharmacology
;
Fibroblasts
;
cytology
;
metabolism
;
Gingiva
;
cytology
;
Humans
;
Lipopolysaccharides
;
Mouthwashes
;
pharmacology
3.Construction of NF-kappaB siRNA expression vector.
Yunusi KUREXI ; Guo-hong HUANG ; Mei LONG ; Jie HE ; Maimaitiaili NASIER ; Yan-ping XIA ; Wufuer HAMULATI
Journal of Central South University(Medical Sciences) 2007;32(5):765-770
OBJECTIVE:
To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein.
METHODS:
pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope.
RESULTS:
The levels of NF-kappaB protein in COS-7 cells could be silenced effectively and specifically by pSilencer 1.0-U6-siRNA- NF-kappa recombinant vector. The expression of NF-kappaB protein was reduced gradually with the increase of pSilencer 1.0-U6-siRNA- NF-kappaB recombinant vector,which could be detected by Western blot under the inverted fluorescence microscope.
CONCLUSION
NF-kappaB siRNA expression vector is constructed successfully.
Animals
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COS Cells
;
Chlorocebus aethiops
;
Genetic Vectors
;
NF-kappa B
;
genetics
;
RNA Interference
;
RNA, Small Interfering
;
Transfection