Objective To investigate the importance of PTEN protein and CD44v6 detection to the prediction of laryngeal carcinoma differentiation and cervical lymph node metastasis. Methods The expressions of PTEN protein and CD44v6 were detected by immunohistochemical SP method in 97 specimens with laryngeal carcinoma and 97 specimens with a distance over 2.5 centimeters from laryngeal carcinoma. The relations between them and their clinical significance to the laryngeal squamous cell carcinoma above Glottis by the statistical analyses tools were revealed. Results The positive expression rates of PTEN protein were respectively 55.67 %, 100 % in laryngeal carcinoma and adjacent carcinoma tissue. There was a significant difference between them statistically (P<0.01). The positive expression rates of PTEN protein are notable difference among the high, middle and low differentiation of laryngeal carcinoma, but not between the positive metastasis of lymph node of neck and the negative one(P >0.05). For the expression of CD44v6, there were notable statistical difference not only among the different differentiation of laryngeal careinoma, but also between the positive metastasis of lymph node of neck and the negative one. The low expressions of PTEN protein and the high expression of CD44v6 in the same carcinoma cell has significant negative correlation (P< 0.05). Conclusion PTEN protein may have the importance to the differentiation of laryngeal carcinoma, but no means to cervical lymph node metastasis. CD44v6 may have great importance to both.

Objective To study the clinical characteristics and treatment of functional articulation disorders. Methods 65 children withfunctional articulation disorders were reported. Results The main erroneous articulation pattern in these children was substitution, followedby omission and/or distortion. They were trained from oral function and articulation motion for 2~6 months. 58 children were corrected completely,and 7 were corrected partially. Conclusion The main erroneous articulation pattern of functional articulation disorder in children issubstitution. Speech training is effective on it.

Objective Tetralogy of Fallo( TOF) is one of cyanotic congenital heart disease, which is difficultly diagonosed because of its complicated texture.The aim of this study was to evaluate the manifestation and value of echocardiography in diagnosis of TOF. Methods The fetal sonograms of 99 fetuses with prenatal diagnosis of TOF in 5791 gravida were reviewed and analyzed from January 2010 to December 2012. Results Ninty nine fetuses with TOF were all diagnosed accurately by prenatal ultrasonography, including 11 cases with pulmonary atresia, 2 cases with complete atrio-ventricular septal defect and 4 cases with permanent left superior vena cava.The key echocardiographic features of TOF were ventricular septal defect, overriding aorta and pulmonary stenosis.TOF was accurately diagnosed by prenatal echoeardiography combined with visualization of TOF in four chamber view, outflow tract view and three vessels view.Sixty eight fetuses were confirmed to be TOF by autopsy or operation after birth, 12 cases refused to autopsy and 19 cases lost of follow up in the total 99 cases.None of TOF was reported to us in other 5692 gravidas. Conclusion The echocardio-graphy has important clinic value in diagnosis of fetal TOF with high sensitivity and specificity.

Basal Ganglia ; pathology ; Female ; Humans ; Rodenticides ; poisoning ; Young Adult

Objective To investigate the effect of different concentrations of arsenic trioxide (As2O3,ATO) on neurokinin A (NKA) in renal tissue of BXSB mice and explore its clinical value.Methods Fifty BXSB mice (twelve weeks old and weighted 23～26 g) were randomly divided into control group,systemic lupus erythematosus (SLE) group,and therapeutic group of three different concentrations of ATO.All biochemical indicators were analyzed before and after treatment.The pathology of renal tissue was examined by immunohistochemistry.The concentration of NKA in renal tissues was detected by ELISA and the concentration of NKA mRNA was detected by RT-PCR.Results The concentration of NKA in SLE group in renal tissue (299±26) pg/g was significantly higher than that of normal control group (122±7) pg/g (P＜0.05).The concentration of NKA in the SLE group in renal tissue was significantly higher than that of three different concentrations of ATO in low-dose group (151±14) pg/g,moderate--dose group (147±9) pg/g and in highdose group (155±14) pg/g (P＜0.05).No difference was found between three different dosages of ATO treatment groups and normal control groups (P＞0.05).There were no significant differences among three different dosages of ATO treatment group (P＞0.05).The side effects in low-dose group were significantly lower than those of moderate and high-dosage groups (P＜0.05).Conclustion NKA concentration expressed in the renal tissues in the SLE group is higher than that in the control group.Decreasing the concentrations of NKA mRNA in renal tissues may be one of the important mechanisms of ATO in treating SLE.Low-dosage ATO is safe and effective to treat SLE and has therapeutic potentials.

Apnea ; epidemiology ; pathology ; therapy ; Birth Weight ; China ; epidemiology ; Female ; Gestational Age ; Humans ; Infant, Newborn ; Infant, Very Low Birth Weight ; Male ; Risk Factors ; Time Factors

LAVP2. Multiple linear regression revealed duration of hypertension, grade of hypertension, age and smoking status were independent determinants for the deterioration of diastolic dysfunction. Conclusion A progressing change in regional diastolic dysfunction from right to left ventricle was found in HT patients. The grade of hypertension and the duration of HT were the major determinants on diastolic dysfunction.

Objective To investigate the changes of molecule markers of pre-thrombotic state,expression of CD62P,concentration of plasma fibrinogen(Fib),prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT) in the patients with essential hypertension for providing theoretical evidence for diagnosis and therapy of thrombus complication in early clinical stage.Methods Fifty-three patients with hypertension(grade Ⅰ 17 cases,grade Ⅱ 16 cases,grade Ⅲ 20 cases)were selected and twenty-eight people with medical examination in good health were used as control group.The expressions of CD62P and CD61 were measured by flow cytometry.The concentration of Fib and the levels of PT,APTT,TT were measured by Class coagulation method.Results The expression of CD62P and the concentration of plasma Fib in essential hypertension group were higher than those in control groups(P0.05).Conclusion The essential hypertensive patients are in pre-thrombotic state,the activation of platelet and blood clotting activity increase.Molecular markers should be measured in essential hypertensive patients for prevention and treatment of thrombotic disease.

Objective To establish the quality standard for Fushenning Tablets. Methods Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae were identified by TLC,and the content of berberine hydrochloride of Cortex Phellodendri Chinensis was determined by HPLC. Results TLC could identify Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae effectively. Berberine Hydrochloride showed a good linear relationship in the range of 0.021 42～ 0.110 20 ? g. The average recovery was 100.6 %(n=6) and RSD was1.4 % . Conclusion This method is simple,accurate,specific and reproducible,and can be used for quality control of Fushenning Tablets.

Objective To investigate the significance of the expression levels of humans telomerase reverse transcriptase(hTERT) gene in peripheral blood mononuclear cells with lung cancer patients. Methods From Mar.2002 to Feb.2005,the expression levels of hTERT were detected in 30 lung cancer patients and 15 normal humans by quantitative measurement with RT-PCR. Results The expression levels of hTERT mRNA in lung cancer patients (0.906?0.112) were significant as compared with those health controls(0.407?0.104). Conclusion It is effective to diagnose lung cancer by the quantitative measurement with RT-PCR for the expression levels of hTERT mRNA.