10 days.All the patients received operations.Results:The prognoses of 80.49% patients in the early and middle stages were well ,the fadeaway of edema was evident,on the contrary,the prognoses of 83.33% patients of the later stage were worse,the fadeaway of edema was slow.Conclusion:The patients of LEDVT should receive operation as early as possibly in 10 days,the patients should receive conservative therapy after 10 days.

Objective To study the value of routine laboratory examinations, symptoms and soluble IL-2 receptor (sIL-2R) in the flare-prediction on systemic lupus erythematosus(SLE). Methods A prospective study was performed in 106 outpatients with SLE and the patients′ conditions were assessed according to Systemic Lupus Erythematosus Disease Activity Index(SLEDAI). Patients were asked to visit at no longer than 3-month interval. All patients were followed-up for 6 to 18 months. At each visit, laboratory examinations, symptoms, disease activity, as well as the changes in the treatment were recorded according to the protocol. The values of these parameters in flare-prediction were comprehensively assessed based on their frequency and time respectively when they became abnormal before the disease flare. Results Among the 106 patients, the disease activity of 67 cases were in remission and satisfied the prospective study criteria of the SLE flare. Thirty-one flares in 29 patients were observed. The frequency of flare was 0.44/patient/year. Comprehensively the most valuable predictor of those parameters was anti-dsDNA antibody, followed by complement components, sIL-2R, erythrocyte sedimentation rate(ESR), complete blood count, urine analysis, fatigue, mucous membrane ulceration, new rashes and alopecia. C3 was a better predictor for SLE flares than that of C4 and CH50. Conclusions The flares of SLE could be predicted. Early intervention will help to prevent patients from flare.

Neurologic complications are rare but devastating events after scoliosis surgery;a thorough preoperative work-up and effective intraoperative monitoring of the spinal cord may reduce the incidence.This review discusses the incidence,preoperative work-up,intraoperative monitoring,and managing strategies for neurologic complications of scoliosis surgery.

Objective To investigate and analyze structure of risk perceived by people using HIV voluntary counseling and testing services and related factors.Method Two hundred and sixty eight VCT users were selected from 2 CDCs and 2 hospitals in Beijing and their perceived risks in the process of VCT were assessed by using self-administered question- naires.Factor analysis was performed to understand the relationship between different perceived risks,and risks perceived by different users were evaluated.Results Six factors were obtained from the factor analysis which included:fear of priva- cy disclosure,embarrassment,concern over the reliability of HIV tests,cost & time consumption,the tragic outcomes of HIV positive tests,and potential medical expenditures in the future.And the risks perceived by low and high income users were much higher than those perceived by middle income users.Conclusion There are clear structure of risks perceived by people using HIV voluntary counseling and testing,and people with different incomes have different levels of perceived risks.

Azabicyclo Compounds ; blood ; Humans ; Piperazines ; blood ; Spectrophotometry, Ultraviolet

Objectives： To identify the peptide sequence of a epitope and study its three dimensional structure on molecule of cyclophilin A.　Methods： Using anti oyelophilin A (CyPA) monoclonal antibody (McAb D4) as the capture antibody, the library displayed random heptapeptides was screened by biopanning. The phage clones with high affinity for McAb D4 were chosen and their inserts were sequenced and then the antibody binding site was searched for on three dimensional structure of CyPA with RasMol software.　Results：After 3 rounds of biopanning, 7 phage clones with high affinity for McAb D4 were sequenced and 3 different species were obtained, five of seven clones shared a predominant consensus sequence, WSLQSFL. The amino acid sequences of the inserts were compared with that of human CyPA.No homologous sequence was found in the primary structure of CyPA. With the data from PDB database and RasMol software, we found that W121,S99,L98,Q111,S110,F112 and L122 constructed a hydrophobic pocket which may be the epitope for McAb D4.　 Conclusions： W121,S99,L98,Q111,S110,F112 and L122 may form a conformational epitope of cyclophilin A.

A study on the optimal dilution of serum to detect anti HBc-IgM with ELISA. was carried out. Samples were diluted to 10-1to 10-7. Among 26 samples of normal individuals, 22 were negative, but two samples of 10-1 dilution, one sample of 10-2, and one sample of 10-1-10-3 were positive though the control wells were all negative. 42 samples of the patients with variours types of hepatitis B were examined. It was found that when serum dilution was 10-1, only 6 cases were positive; when the dilution was 10-2, 20 cases were positive; when the dilutions were 10-3, 5?10-3, and 10-4, high positive rates were obtained and they were 88.1%, 78.5%, and 69% respectively; and when the samples were further diluted, the positive rate decreased.In order to get goodfresults and minimize false results, either false negative or false positive, it is suggested that 5?10-3 be the optimal dilution.

Based on the theory and approach of International Classification of Functioning, Disability and Health (ICF), the literatures on the improvement of physical function by physical exercise were reviewed. People with disabilities can improve their body functions, in-cluding mental function, cardiovascular, blood, immune and respiratory function and neuromuscular skeletal and motor related functions via sport and exercise rehabilitation. Some advice was suggested about sports rehabilitation for the disabled.

Ossification of the posterior longitudinal ligament (OPLL) is common clinical spinal disorders often occurring in the cervical spine,with the main symptom being nerve compression.The specific mechanism of OPLL remains unclear,but genetic factors,single nucleotide polymorphisms (SNPs),mechanical stimulation,metabolism abnormality might be involved in the etiology of the disease.Multiple genetic and environmental factors may contribute to the development of OPLL.OPLL has prominent genetic characteristic,and it is associated with SNPs of several genes.Here we review the SNPs of several genes (COL11A2,BMP-2,TGF-?1,TGF-?3,NPPS,COL6A1 and Runx2) which contribute to the development of OPLL,hoping to lay a foundation for future study.