OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Objective To analyze the mechanism of acute ischemic stroke in cancer patients. Methods The clinical TOAST type, laboratory examinations results, imaging examination results and pathology reports of the 96 patients with cancer and acute cerebral infarction were collected. Results According to TOAST mechanism, the main subtype was undetermined causes (41.67%, 40/96). Lung cancer took largest proportion (39.58%, 38/96). In the group of undetermined causes, adenocarcinoma was more common type than squamous. The imaging examination results could be classified into large artery type, large artery and small artery in same basin and multiple small arterys in different basins. We found the main type was multiple small arterys in different basins (52.50%, 21/40). The lever of D-dimer was high in our patients, especially in undetermined causes (P < 0.01). Conclusions Tumor-related acute cerebral infarction has its own uinque mechanism. Tumor is one kind of systemic diseases, which can promote thrombosis through increasing the lever of D-dimer. The cancer-related mechanism is the main mechanism underlying cancer- related stroke, and may become one of the most important mechanismsn in the pathogenesis of stroke in the near future.

Objective To study effect of positive liver soup on serum leptin (LEP), adiponectin (ADP) and insulin resistance (IR) in patients with hepatitis B cirrhosis.Methods 68 cases of hepatitis B patients with cirrhosis according to randomized divided into the control group (34 cases) and treatment group (34 cases).Oral treatment administered to the control group (Inosine Tablets and vitamin C),treatment group in the control group based on liver soup treatment, treatment for 3 months, serum leptin (LEP),adiponectin (ADP) and insulin resistance (IR) index before and after treatment were detected.Results Before treatment, there was no significant difference between ADP and LEP in the control group and the treatment group.After 3 months of treatment, there was no significant difference between ADP and LEP level in control group, while the level of ADP and LEP in treatment group were significantly decreased (P<0.05), and were significantly lower than that in control group (P<0.05).There was no significant difference in FPG, fasting insulin level and IRS index between the control group and the treatment group before treatment.After 3 months of treatment, the control group FPG, fasting insulin level and IRS index were not significantly different.FPG, fasting insulin and IRS index in the treatment group were significantly decreased (P<0.05), and were significantly lower than those in the control group (P<0.05).Conclusion Positive liver soup can reduce serum LEP, ADP level, and improve IR in treatment of hepatitis B liver cirrhosis.

Objective To investigate the correlation between hyperuricemia and obesity in children.Methods 316 children who went to the hospital for treatment of obesity were enrolled in the study as obesity group.Age and sex-matched children with normal body mass index(BMI)were randomly recruited as control group.Fasting venous blood samples were collected for the detection of serum uric acid(UA),glucose(GLU),total cholesterol(CHOL),triacylglycerol (TG),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C).Results Compared with the control group,serum concentrations of UA,CHOL, TG and LDL-C were higher while HDL-C was lower in obese children,the difference was statistically significant(P <0.05 ).The detection rate of hyperuricemia in obese children was 24.68%,and in control group was 9.49%,the difference was statistically sig-nificant(P <0.05 ).Serum UA concentration was positively correlated with TG,CHOL and LDL-C,negatively correlated with HDL-C.Conclusion Obesity can lead to higher serum UA concentration and abnormal lipid metabolism.The level of serum UA was correlated with abnormal lipid metabolism.

Objective: To provide policy recommendations for improving price negotiation system of patented drugs in China. Methods: This paper comparatively analyzes the commonalities and characteristics between Korea’ and Germany’s price negotiation models for patented drugs from three aspects of their goals, procedures and effects. Results:The key objective of both Korea’ and Germany’s price negotiation systems for patented drugs is to efficiently improve the utilization of national health insurance services, and responsibilities are properly assigned among differ-ent institutions to ensure the equity and efficiency of negotiations. However, due to the differences in national cir-cumstances, there is a big difference in the selection of technical assessment criteria. Conclusion: This paper sug-gests China to strengthen the convergence between price negotiations for patented drugs and medical insurance reim-bursement policy, to establish a technical assessment system with the innovation extent for patented drugs as its core, and to develop scientific and rational negotiation procedures and division of responsibilities as well.

Objective:To suggest approaches for the establishment of Chinese standards for defining rare disea-ses and advance corresponding Chinese legislation. Methods:By comparing the standards for defining rare diseases in Europe, the United States, and other countries and studying key influencing factors of standards, this paper proposes suggestions for the establishment of proper Chinese standards for defining rare. Results and Conclusion:Social and e-conomic development levels, medical development levels and social security levels influence the establishment of standards. It is recommended that the national conditions should be taken into consideration and the number of pa-tients, severity of the diseases and economic indicators of orphan drugs be used to define rare diseases in China.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

Objective To establish the quality standards for Shufeng Zhike Extract;To improve the controll ability of the extract and ensure medicine efficacy. Methods To establish a HPLC method for simultaneous differentiation of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum in Shufeng Zhike Extract. Phenomenex Kintex C18 column (4.6 mm×100 mm, 2.6 μm) was used. Acetonitrile-0.05%phosphoric acid was as the mobile phase in gradient elution (0–25 min, 10%acetonitrile; 25–26 min, 10%→14% acetonitrile; 26–35 min, 14% acetonitrile; 35–36 min, 14%→34% acetonitrile;36–55 min, 34%acetonitrile) at flow rate of 0.8 mL/min, and column temperature was 40 ℃. The detection wavelength was 250 nm. To establish a HPLC method for simultaneous determination of Paeoniflorin and Prim-O-glucosylcimifugin in Shufeng Zhike Extract, Phenomenex Luna C18 column (4.6 mm×150 mm, 5 μm) was used; acetonitrile-0.05%phosphoric acid (12:88) was as the mobile phase at flow rate of 1 mL/min; column temperature was 40 ℃; the detection wavelength was 250 nm. Results The chromatographic peak separation with HPLC method for simultaneous differentiate of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizoma et Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurumin in Shufeng Zhike Extract was clear and negative control had no interference. The linear range of Paeoniflorin was 0.035 93–2.514 8 μg (r=0.999 5), and the average recovery was 100.54%(n=6). The linear range of Prim-O-glucosylcimifugin was 0.006 7–0.67 μg (r=0.999 5), and the average recovery was 100.39%(n=6). Conclusion The established quality standards are simple, reliable, and accurate. It can rapidly identificate Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum, and suitable for rapid determination of Paeoniflorin and Prim-O-glucosylcimifugin, which can provide the basis for the quality inspection of Shufeng Zhike Extract.

OBJECTIVE:To study the effect of Yupingfeng(YPF)extract on Ca,P,Mg and hydroxyproline of bone in model rats with osteoporosis induced by cyclophosphamide(CP)and to discuss the preventive and therapeutic effect of YPF ext-ract on CP-induced osteoporosis.METHODS:A total of 40 rats were randomly divided into four groups:control group,CP group,YPF extract group,and calci?m carbonate + Vit D group.The rats were sacrificed at 15 days of experiment,and the levels of Mg,Ca,P and hydroxyproline of left femoral bone were determined.RESULTS:Compared with control group,levels of different index with CP group was significantly decreased;compared with CP group,levels of Ca,Mg,P and hydroxyproline of bone in rats treated with YPF extract were significantly increased.CONCLUSION:CP may induce decrease of levels of Ca,Mg,P and hydroxyproline of bone,however,on which YPF extract has preventive and therapeutic effect.