OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use

This review snmmarized and estimated the management of cataract blindness in China recently,and introduced the purport of clinic medical economics.Based on it,it was proposed that our service system of cataract blindness could use the experiences of other countries as reference and engaged in more investigations in search of Chinese way for cataract blindness.

Objective To study effect of positive liver soup on serum leptin (LEP), adiponectin (ADP) and insulin resistance (IR) in patients with hepatitis B cirrhosis.Methods 68 cases of hepatitis B patients with cirrhosis according to randomized divided into the control group (34 cases) and treatment group (34 cases).Oral treatment administered to the control group (Inosine Tablets and vitamin C),treatment group in the control group based on liver soup treatment, treatment for 3 months, serum leptin (LEP),adiponectin (ADP) and insulin resistance (IR) index before and after treatment were detected.Results Before treatment, there was no significant difference between ADP and LEP in the control group and the treatment group.After 3 months of treatment, there was no significant difference between ADP and LEP level in control group, while the level of ADP and LEP in treatment group were significantly decreased (P<0.05), and were significantly lower than that in control group (P<0.05).There was no significant difference in FPG, fasting insulin level and IRS index between the control group and the treatment group before treatment.After 3 months of treatment, the control group FPG, fasting insulin level and IRS index were not significantly different.FPG, fasting insulin and IRS index in the treatment group were significantly decreased (P<0.05), and were significantly lower than those in the control group (P<0.05).Conclusion Positive liver soup can reduce serum LEP, ADP level, and improve IR in treatment of hepatitis B liver cirrhosis.

OBJECTIVE:To provide references for the improvement of civil relief system for adverse drug reactions.METHODS:The approaches for Japan in handling the "quinoform" drug injury event were compared with the way of China in dealing with drug injury events.RESULTS & CONCLUSION:The approaches for Japan in handling the"quinoform" drug injury event give some hints for China,i.e.the defective drugs should be comprehensively judged by judges,the causal relations should be determined by using the probability theory and the time point of producers' exemption should be revised.

OBJECTIVE:To evaluate the application effects of PDCA cycle management in the outpatient prescription intervention.METHODS:A total of outpatient prescriptions (pre-intervention group) during Jan.-Dec.2014 and 918 659 (intervention group) were selected during Jan.-Dec.2015 were selected from a hospital.Irrational 9utpatient prescriptions were analyzed before and after the application of PDCA cycle management,and the improvement of main indexes were compared such as unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample.RE SULTS:Before intervention,there were 2 347 irrational prescriptions,including 1 401 nonstandard prescriptions,849 unsuitable prescriptions and 97 abnormal prescriptions.After intervention,there were 1 161 irrational prescriptions,including 695 nonstandard prescriptions,425 unsuitable prescription and 41 abnormal prescriptions,decreasing 50.53％,50.39％ 49.94％ and 57.73％,respectively.The proportion of unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample,nonstandard prescription paper,unsuitable indications,no signature and date for prescription revision or no reason and new signature for overdose use,outpatient prescriptions more than 7 d common dose or emergency prescriptions more than 3 d common dose without special situation in total amount of outpatient prescriptions decreased from 0.72‰,0.57‰,0.45‰,0.27‰,0.20‰,0.19‰ and 0.15‰ before intervention to 0.32‰,0.25‰,0.19‰,0.11‰,0.09‰,0.08‰ and 0.07‰ after intervention.CONCLUSIONS:PDCA cycle management significantly improves the quality of outpatient prescriptions.There still are irrational outpatient prescriptions in this hospital,and it is to be intervened continuously.

Objective To explore the effect of the severity of coronary artery on left ventricular diastolic function in patients with unstable angina (UA).Methods One hundred and seventy-two patients with UA consecutively from December 2010 to August 2011 were enrolled.All patients underwent coronary angiography (CAG) and echocardiography.The patients were divided into four groups according to left ventricular diastolic function by echocardiography:Thirty-eight subjects with normal(Non-HFNEF),45 subjects with mild dysfunction (Grade Ⅰ),57 subjects with moderate dysfunction(Grade Ⅱ) and 32 subjects with severe dysfunction grade (Grade Ⅲ).The effect of the severity of coronary artery on the left ventricular diastolic function was analyzed.Results There were significant difference on left atrial dimension (3.24 ± 0.50) nn vs (3.40 ±0.41) mmvs (3.56±0.44) mm vs (3.78 ±0.36) mm (F=21.454,P＜0.001),left ventricular posterior wall (0.84 ±0.11) mm vs (0.90 ±0.12) mm vs (0.92 ±0.09) ram vs (0.94 ±0.15) mm (F =10.743,P ＜ 0.001) and left ventricular mass index (105.63 ± 33.13) g/m2 vs (113.09 ± 21.55) g/m2 vs (122.26 ±41.52) g/m2 vs (132.96 ± 31.06) g/m2(F =9.746,P ＜ 0.001) among the four groups.Diastolic function was significantly correlated with the severity of coronary artery(r =0.466,P ＜ 0.001).Conclusion In the patients with UA,left ventricular diastolic function was significantly influenced by the extent of coronary artery stenosis.

Objective: To analyze the clinical characteristics of non-diabetic fetal macrosomia and their mothers.Methods: A retrospective case control study design was used to analyze 149 cases of non-diabetic fetal macrosomia and their mothers with the normal oral glucose tolerance test(OGTT) by comparison with 166 randomly included newborns of normal birth weight.Results: More cases of non-diabetic fetal macrosomia were found in the male neonates.The body weight,height,FL and BPD averaged significantly higher in the macrosomia group than in the control,and so did the body weight,height,uterus height,abdominal circumference and gestational age of the mothers.The rates of cesarean section and other obstetric complications were also higher in the macrosomia group.Conclusion: Different from the diabetic macrosomic fetus,the non-diabetic macrosomic fetus is a normal one,the incidence of which is associated with mothers'excessiver pregnancy nutrition as well as with genetic factors.

Objective To comprehend the etiology and the clinical characteristics of hypereosinophilia.Methods The clinical data of 53 patients with hypereosinophilia admitted to Peking Union Medical College Hospital from November 1992 to February 2005 were analyzed.Results The causes of reactive hypereosinophilia included allergic diseases,infections,connective tissue diseases,malignant diseases,lung diseases,eosinophilic gastroenteritis.idiopathic hypereosinophilic syndrome had longer course of disease;more organ involved than reactive causes.The incidence of lymph node enlargement,spleen enlargement and thrombus was higher than the reactive.IHES had higher level of white blood cell count,absolute eosinophilic count,IgG and CRP.Conclusion The etiology and clinical characteristics of hypereosinophilia are various,so the differential diagnosis of hypereosinophilia is very important.