Objective To study the expression of Ephrin‐B4 receptor (EphB4) in hepatocellular carcinoma (HCC) and its clinical significance ,and to analyze the effect of EphB4 on the proliferation of HCC cells .Methods The expression level of EphB4 in HCC tissues and matched paracancerous liver tissues of 60 cases of HCC patients was assessed by reverse transcriptase‐polymer‐ase chain reaction (RT‐PCR) and immunohistochemistry .The correlation between the expression of EphB4 in HCC tissues and clin‐ical pathologic parameters was analyzed by chi‐square test .Univariate survival analysis was carried out by Kaplan‐Meier Log‐rank test .The effect of EphB4 on the viability of HCC cells was furtherly analyzed by MTS .Results The results of RT‐PCR showed that the mRNA level of EphB4 in HCC tissues (1 .39 ± 0 .80) was significantly higher than in matched paracancerous liver tissues (0 .56 ± 0 .33) ,the difference was statistically significant (P< 0 .01) .Data from immunohistochemistry showed that the positive rates of EphB4 protein in HCC tissues and matched paracancerous liver tissues were 81 .7% and 23 .3% ,respectively .Moreover ,the expression of EphB4 in HCC tissues was relevant to AFP level ,tumor size and TNM stage(P<0 .05) .The three‐year survival rate of HCC patients with positive expression of EphB4 protein was 22 .5% ,and that of HCC patients with negative expression of EphB4 protein was 54 .5% ,the difference was statistically significant (P<0 .05) .Overexpression of EphB4 significantly enhanced the ability of hepatocellular carcinoma cell proliferation (P<0 .05) .Conclusion EphB4 expression was significantly up‐regulated in HCC ,which was associated with HCC progression and prognosis ,and EphB4 could promote the proliferation of HCC cells ,which could be used as a marker of HCC progression .

OBJECTIVE To acknowledge the important factor of bacterial infection of patients after tracheotomy. METHODS Thirty five patients′ lower respiratory tract sputa were sent to conduct bacterial culturing and determine their susceptibility to antibiotics and analyze results with statistics methods. RESULTS Total 8 main kinds of bacteria were isolated and drug resistance rate of Gram-positive cocci over 50 percent was erythromycin,that of(Gram-negative) bacilli included ampicillin,gentamicin,ciprofloxacin,and cefuroxime.The average time of antibiotics usage among 35 patients was(27?19.17) days and 12 kinds of antibiotics were used. CONCLUSIONS The time of usage of different antibiotics is an important factor to aggravate patients′ infection after tracheotomy.

Objective To evaluate the role of MRA,IPVG and DUS in the hemodynamics studies of portal hypertension.Methods Thirty-eight patients with portal hypertension were examined with Philips Gyroscan 1.0 Tesla MR imaging system.3D-DCE MRA and 2D-PC MR were used for study of portal venous anatomy and its hemodynamics.The results were compared with those obtained from IPVG and DUS.Results 3D-DCE MRA could clearly display the anatomical imaging of portal venous system and its imaging quality was better than that of IPVG.The data of hemodynamics from 2D-PC MR including diameter,blood velocity and blood flow were closely correlated to those from DUS.Conclusion As a non-invasive technique,MRA can display the anatomy of portal venous system and measure its hemodynamics exactly.It should be applied as the first choice in hemodynamics evaluation for portal hypertension.

Objective:To study the dynamic frictional resistance in the buccal segment between preadjusted brackets coupled with different archwires,at different times under artificial saliva condition.Methods: The dynamic friction of different combinations of 4 kinds of preadjusted brackets and 4 archwires were tested simulating archwires sliding in the buccal segments at 4 different times(0,15,30,45 d)under artificial saliva condition by using Instron universal testing machine.Results: The dynamic frictional resistance of combinations of 3M MBT bracket and all archwires were the smallest.The combinations of 0.46 mm inch stainless steel round wire and all brackets produced significantly lower dynamic frictional resistance than that of other archwires.The 0.48 mm?0.64 mm inch stainless steel rectangular wire produced the highest dynamic frictional resistance combined with all preadjusted brackets.The dynamic frictional resistance of combinations of archwires and brackets were highest at the 15th day and smallest at the 30th day under artificial saliva condition.Conclusion: 0.46 mm stainless steel round wire produces the smallest dynamic frictional resistance combined with all preadjusted brackets.3M MBT brackets are more favorable to sliding mechanics compared with other brackets.As for time,the lowest dynamic frictional resistance exhibits at the 30th day under artificial saliva condition.

Adenocarcinoma ; diagnosis ; therapy ; Guidelines as Topic ; Humans ; Thyroid Neoplasms ; diagnosis ; therapy

Adenocarcinoma, Clear Cell ; diagnosis ; metabolism ; pathology ; Adenocarcinoma, Mucinous ; diagnosis ; metabolism ; pathology ; Biomarkers, Tumor ; metabolism ; Carcinoma, Endometrioid ; diagnosis ; metabolism ; pathology ; Cystadenocarcinoma, Serous ; diagnosis ; metabolism ; pathology ; Diagnosis, Differential ; Endometrial Neoplasms ; diagnosis ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; Uterine Cervical Neoplasms ; diagnosis ; metabolism ; pathology

Objective To study the influence of combined anti-CD-40L and low dose CsA immunotherapy on the induction of immune tolerance in orthotopic liver transplantation rats.Methods Recipients were divided into the following groups.Group A,SD→SD;Group B,SD→Wistar without any treatment;Group C,SD→Wistar with CsA therapy from d1 to d5;Group D,SD→Wistar with CsA from d1 to d5 and anti-CD40L mAb in d0 and d2,and Group E,SD→Wistar with CsA from d1 to d5 and anti-CD40L mAb in d0 and d2 plus autotransfusion before operation.The recipient survival time,histologic changes of allograft and the level of cytokines in peripheral blood were observed.Results The survival period of recipients in Groups A,D and E was significantly longer than that in Groups B and C.The acute rejection response in Groups D and E decreased prominently.The serum level of IL-2 and IFN-? in Group B were significantly higher than those in other groups,while the serum level of IL-4 and IL-10 in Groups D and E was elevated much more than those in Group B(P

Objective To investigate the correlations between plasma levels of carnitine(CT),free fat acid(FFA) and insulin resisitance in children with simple obesity.Methods Fifty-six children diagnosed with simple obesity were enrolled as study group(obesity group),36 healthy children as control group.The concentration of plasma level of insulin was measured by radioimmunoassay(RIA),CT was measured by high performance liquid chromatography(HPLC),FFA and triglyceride(TG) were measured by enzymatic-colorimetric assay.Body mass index(BMI) and waist to hip ratio(WHR) were caculated.Insulin sensitivity index (InSI) and insulin resistance index (InRI) were cacula-ted by homeostasis model assessment of insulin resistance (HOMA-IR).SPSS 13.0 software was used to analyze the data.Results The concentration of CT in plasma was (43.67?12.75) ?mol/L in obesity group,(58.31?21.25) ?mol/L in control group,respectively.There was a significant difference between 2 groups (t=2.566 P

The imaging appearances of 99Tc(m)-HL91, a new hypoxic imaging agent, in ischemic myocardium were studied and the value of 99Tc(m)-HL91 in the evaluation of regional ischemic viable myocardium was explored. Acute myocardial ischemia models were made by coronary artery legations in 18 rats and randomly divided into 2 groups: 99Tc(m)-HL91 group and 99Tc(m)-MIBI group. Evan blue infusion during ischemia and TTC staining after operation were used to delineate the area of ischemic and viable myocardium. The isolated heart was sliced in the short axis and then autoradiography was performed. The electron microscopic examination was also done for the myocardial samples. 99Tc(m)-HL91 and 99Tc(m)-MIBI uptake activities (counts/g) were measured in the area of ischemic myocardium (T) and normal myocardium (NT) separately. The uptake ratios of 99Tc(m)-HL91 and that of 99Tc(m)-MIBI in ischemic myocardium were calculated as T/NT. It was found that the normal myocardium was blue and ischemic or infarct myocardium was negative with Evans blue in all experiment rats. Both the normal and ischemic myocardium was in red color with TTC staining. In the 99Tc(m)-HL91 group the ischemic myocardium showed much higher uptake over normal myocardium, that was demonstrated both in the autoradiography and quantitative analysis. The ischemic/normal activity ratios were 1.634 +/- 0.354. It was suggested that 99Tc(m)-HL91 might accumulate in ischemic and viable myocardium, which is helpful in the evaluation of hypoxic but viable myocardium and potentially used as a imaging agent to assess myocardial viability.

AIM To study the protective and antiperoxidative effects of baicalin on myocardial ischemia. METHODS The rat anterior decenging branch of left coronary artery was occluded for 40 min and reperfusion for 120 min. Hemodynamics (LVP, LV d p /d t ) and electrocardiogram (ECG, lead Ⅱ) monitored continuously with polygraph,then the myocardium were taken to assay lactate dehydrogenase(LDH), malondialdehyde (MDA) contents and superoxide dismatase (SOD). RESULTS Baicalin 10 or 40 mg?kg -1 iv depressed changes in LDH, MDA and SOD in myocardium after ischemia for 40 min and reperfusion for 120 min in rats. Baicalin 10 and 40 mg?kg -1 iv markedly improved the ?d p /d t max (kPa?s -1 ), LVSP in rat after ischemia for 40 min and reperfusion. CONCLUSION Baicalin possesses a protective effect against myocardial ischemia via attenuating lipid peroxidation.