Objective To evaluate the radiofrequency ablation in treatment of primary and recurrent liver cancer and to explore the risk factors affecting prognosis.Methods 1 64 patients with primary and recurrent liver cancer underwent radiofrequency ablation treatment.The clinical data including liver function before and after treatment,alpha-fetoprotein (AFP),the rate of relapse and survivalwere retrospectively analyzed.The univariate Logistic regression analysis was used to analyzed the risk factors associated with the prognosis.Then the risk prognostic factors were analyzed using COX risk model.Results After treatment,AFP and liver function were significantly improved in patients (P<0.05);Complete tumor ablation rate was 87.50%.After cancer treatment 1,2,3,4 years,the recurrence rate was 17.9%,29.9%, 45.9% and 55.0% respectively.Conclusion Radiofrequency ablation is an effective treatment for primary liver cancer treatment,the risk factors affecting prognosis include tumor differentiation,tumor recurrence,TNM stage,AFP,intraoperative blood transfusion, surgical,cancer and surgical site before surgery transaminase levels.

The development of Lab Imformation Online Query System is fulfilled based on Single Version LIS,and thus the real-time share and inquiry of lab information can be performed.

Objective To clone human vascular endothelial growth factor 165(hVEGF_ 165 ),construct its eukaryotic expression and to study the expression of hVEGF_ 165 . Methods Human vascular endothelial growth factor cDNA was amplified by PCR method from the HL60 cells and cloned to expression vector pcDNA_3,constructed pCD-hVGEF_ 165 recombinant plasmid, then transformed to E.coli BL21(DE3)cell.Results The cloned cDNA was confirmed to be VGEF_ 165 cDNA. It was observed that the expression of human VEGF gene was detected distinctly 72h after transferring.Conclusions We successfully cloned and expressed hGEF_ 165 gene, which provided the further foundation of the model of VEGF transgenic animal and makes a basis for the further study in retinal neovascularization.

Objective To explore the optimal method of qualitative and quantitative assessment of retinal neovascularization(RNV),choroidal neovascularization(CNV) and leakage from CNV. Methods Thirteen mice were randomly divided into RNV assessment group(n=7)and CNV assessment group(n=6).For RNV assessment, 3 mice were served as normal controls,and the other 4 were made into models of retinopathy of prematurity(ROP).At postnatal d16,mice in normal control group and 3 ROP model mice received intraocular injection with FITC labeled or unlabeled antibody and were sacrificed 12 h later.The retinas were flat mounted(FITC labeled antibody) or incubated with secondary antibody(unlabeled antibody) and then flat mounted.The other ROP model mouse was served as blank control.For assessment of CNV and leakage from CNV,6 mice(12 eyes) were induced into models of CNV,and were randomly divided into two groups.Seven and 14d after model establishment,mice were intraocularly injected with unlabeled antibody,and fluorescein sodium were intraperitoneally injected 12 h later.The area of CNV and that of CNV leakage were calculated. ResultsRetinas from eyes injected with FITC labeled antibody exhibited good resolution of ultrastructure of RNV,while retinas from eyes injected with unlabeled antibody showed selective staining of RNV with no background,greatly facilitating identification and quantification of RNV.Merging images of stained CNV with images of fluorescein sodium leakage from CNV revealed double labeled CNV surrounded by fluorescein that had leaked into surrounding tissues.The mean area of CNV and its leakage of d14 were significantly different from those of d 7(P

Catheter Ablation ; methods ; Child ; Epicardial Mapping ; Female ; Heart Aneurysm ; complications ; surgery ; Humans ; Tachycardia, Ventricular ; complications ; surgery

Objective To evaluate the reliability and validity of Diabetes Diet-Related Quality-of-Life Scale (DDR-QOL) in Chinese version,in order to provide an efficient instrument for assessing the quality of life of diabetes in diet therapy.Methods Translating and edit the DDR-QOL scale initially,275 eligible subjects were invited to the study.DDR-QOL scale and 36 item Short Form Health Survey (SF-36) were used to collect data which were conducted for evaluating the internal consistency,reproducibility,construct validity,convergent and discriminant validity of DDR-QOL.Results The total Cronbach's α was 0.86.The factor analysis suggested good construct validity of DDR-QOL.Significant correlations were found between DDR-QOL and SF-36.The quality of life of diabetes who is on the job or not can be identified by DDR-QOL.Conclusions The Chinese version of DDR-QOL is a valid and reliable tool to assess the quality of life of diabetes who are in diet therapy.

Objective To detect cardiac function in rabbits models with isehemic reperfusion and to evaluate the short-term protective effects of ischemic postconditioning on regional systolic function of left ventricle by echocardiography. Methods Twenty-eight rabbits were divided into 2 groups:control group and ischemic postconditioning(IP) group. Echocardiography were performed before tests and 2 weeks after tests. The conventional echocardiography indices included left ventricular end-diastolic diameter(LVDd) ,left ventricular anterior wall end-diastolic thickness (AW) and left ventricular ejection fraction (LVEF). Quantitative tissue velocity imaging and strain rate imaging indices included the peak velocity in systole(Vs) and radial peak strain rate in systole(SRs). Results Before tests, there were no difference in conventional echocardiography indices between the two groups; compared with before test, the two groups had significantly larger LVDd,thiner AW and lower LVEF(P <0.05) ; compared with the controls, the group IP had significantly smaller LVDd, thicker AW and higher LVEF 2 weeks after tests(P<0.05) ; compared with controls, the number of segments with abnormal wall motion in group IP significantly decreased. Before tests,there were no differences in Vs and radial SRs between the two groups; compared with before test,the two groups had significantly lower Vs and radial SRs(P<0.05) ; compared with the controls,Vs and radial SRs in group IP significantly increased 2 weeks after tests (P<0.05). Conclusions Ischemic postconditioning can protect regional systolic function of rabbits models with ischemic reperfusionin in short-term,which can be effectively evaluated by strain rate imaging.

Objective To summarize the therapeutic effect of recombinant human growth hormone and early enteral nutrition in severely burned patients. Methods 35 cases were randomly divided into group EN and group PN.Group EN was treated with early enteral nutrition(EEN) and recombiant human growth hormone(rhGH) ,while group PN was given parenteral nutrition. The plasma levels of pre-albumin (PA), C-reactive protein (CRP) , tunmor necrosis factor α(TNFα) ,fasting blood glucose(FBG) were measured after burn injury. Comparision was made in length of hospital stay, wound healing time,incidence of burn sepsis, gastrointestinal stress ulcer bleeding and enteral nutrition intoleranc. Results Incidence of burn sepsis,gastrointestinal stress ulcer bleeding and enteral nutrition intoleranc in group EN were significantly lower than those of group PN(P ＜ 0.05). The serum levels of CRP、TNFα、FBG in group EN were significantly lower than those of group PN (P ＜ 0.05). The serum PA was decreased at all times in two periods and was markedly decreased in group EN(P ＜0.05). Length of hospital stay and the healing time of donor site, deep partial thickness burn wound, skin transplantion area were significantly shortened in group EN compared with group PN. Conclusion Early enteral nutrition was beneficial for the improving of nutrition state of the burn patients,and the reduction of the incidence of burn sepsis and the hospitalization time.

OBJECTIVE:To determinate vitamin B1 and vitamin B6 in Gengnianling capsules by HPLC simultaneously .METHODS: The separation was performed on Hypersil-ODS C18 column, methanol - sodium hexanesulfonate solution(20 : 80) was used as mobile phase with a flow rate of 0.8ml/ min and detection wavelength of 280nm.RESULTS: Linear correlations with peak area scores were achieved when the sample size of vitamin B1 and vitamin B6 were with a range of 0.884?g-2.652?g (r = 0.9 999) and 0.714?g-2.142?g(r = 0.9 999) .respectively, the average recovery of which were 95.87%(RSD = 0.82%) and 101.96% (RSD = 0.86%), respectively .CONCLUSION: The method is simple, accurate and it can be used for quality control of Gengnianling Capsule.

Objective To evaluate the diagnostic value of 18F-FDG PET/CT in patients with pancreatic neoplasm.Methods Fifty-three patients (36 males,17 females; age:(60.3±8.9) years) who underwent 18F-FDG PET/CT examination for suspected pancreatic tumor were retrospectively analyzed.Thirtytwo of them underwent dual-time point imaging.The characteristics of PET/CT images and serum CA19-9 were reviewed.Pathological results were used as a gold standard for evaluating the diagnostic value of PET/CT.Two-sample t test,paired t test and ROC curve analysis were used for data analysis.Results Thirtynine patients were finally diagnosed with pancreatic cancer and the other fourteen patients with benign disease.With SUVmax cutoff value of 3.13,the sensitivity and specificity of single-time point 18F-FDG PET/CT for diagnosis of pancreatic cancer were 92.3％ (36/39) and 9/14,respectively.The SUVmax was significant different between pancreatic cancer group and benign disease group (6.16±2.89 vs 3.37± 1.58; t =4.46,P＜0.01).Combined with the level of CA19-9,the diagnostic sensitivity and specificity were 69.2％ (27/39)and 13/14,respectively.The SUVmax of the early scan (5.45±2.43) was significantly different from that of the delayed scan (6.87±2.93) in pancreatic cancer group (t =8.25,P＜0.01),whereas no difference could be found in the benign group (3.18±1.28 vs 4.18±2.49; t=2.24,P＞0.05).With the SUVmax cutoff value of 3.3,the sensitivity,specificity and accuracy in the early scan were 87.0％ (20/23),6/9 and 81.2％ (26/32),respectively.While with the SUVmax cutoff value of 3.0,the sensitivity,specificity and accuracy in the delayed scan were 95.7％ (22/23),5/9 and 84.4％ (27/32),respectively.Conclusion 18 F-FDG PET/CT is a useful tool in the differential diagnosis of benign from malignant pancreatic diseases.