This paper summarizes the recent 6～7 year advances in the research of pharmacological effects of polyactin including mainly six aspects: ①Effects of polyactin on functions of macrophages in vitro ; ② Mechanism enhancive effect ofpolyactin on NK cell activity; ③Effects of polyactin on the human lymphocyte immunofunction; ④Effects of polyactin on the proliferation and cytotoxicity of LAK; ⑤Enhances in specific antitumor activity in mice in vivo ; ⑥Effects of polyactin on erythrocyte deformability and immunofunction in Rabbit and Rat.

Objective:To evaluate the effectiveness on the prevention of behavior problems of life skills education combining school-based and parent-involved approaches for third-grade students in China.Methods:This research was targeted at the population of third-grade children in two elementary schools in Qinhuangdao City,Hebei Province.Nine regular school classrooms were randomly divided into three groups:the intervention group(n=208),internal control group(n=209) and external control group(n=204).The intervention included 26-hour competence promotion for students and 5-hour parent training.The Rutter Scale by parent and teacher were used to evaluate the effects at pretest,posttest and 6-month follow-up.Results:Improvement was observed among children in the intervention group than those in the control groups.The statistical difference was significant(P

Objective To evaluate the clinical effects of sinus tarsi approach and eight-shape approach in the treatment of calcaneal fractures.Methods Analyzed the outcomes of 23 patients(21 males and 2 females)with calcaneal fractures(25 feet)treated with plate via sinus tarsi approach and eight-shape approach.The mean age of patients was 38 years old(ranged from 24 to 55 years).According to Sanders classification:16 feet in type Ⅱ,9 feet in type Ⅲ.Results The average operation time was 85 min(ranged from 70 min to 100 min),and no wound compli-cations occurred.X-ray review showed that bony healing,and healing time was 3-6 months.The average duration of follow-up was 13 months(ranged from 12 to 18 months).The result was excellent in sixthteen cases,good in six cases and fair in one case according to Maryland score.The satisfactory rate was 95 .6%.Conclusion Open reduction and internal fixation via the sinus tarsi approach and eight -shape approach provides not only reliable fixation,but also less complications.

Objective To study the clinical effect of manipulation in the treatment of cervical vertigo due to atlantoaxial joint disorder. Methods A total of 86 patients in our hospital from year of July, 2010 to 2014, with cervical vertigo due to atlantoaxial joint disorder were divided into a control group and a treatment group randomly, with 43 patients in each group. The control group was treated by intravenous dripping of betahistine hydrochloride sodium chloride injection, while the treatment group was additionally treated by manipulation on the basis of the control group. The efficacy and symptoms changes together with average blood flow rate of LVA, RVA, BA, and the value of ADI, VBLADI, and DO were compared between the two groups after one week of the treatment. Results After the treatment, the improvement of vertigo and symptoms of the treatment group was between than the control group(t=12.655, P<0.05); the value of ADI and VBLADI decreased lower in the treatment group than the control group (t were 2.888 and 5.334 respectively, P<0.05);Vm of RVA, LVA and BA increased higher in the treatment group than the control group (t were 4.710, 3.534 and 5.335 respectively, P<0.01). Conclusions Manipulation combined with intravenous dripping of betahistine hydrochloride sodium chloride injection is effective in improving vertigo, decreasing ADI and VBLADI, and correcting atlantoaxial joint disorder.

Objective To study and report 7 novel mutations and 5 novel polymorphisms of Wilson disease (WD) geneIn combination with the mutations and polymorphisms previously reported, mutation characteristics of WD gene in Chinese were further analysed. Methods Genomic DNA of 60 normal controls and 84 WD patients from 64 families were extracted from peripheral blood leukocytesThe mutations of WD gene (exon1～21) in these subjects were screened by PCR-single strand conformation polymorphism (SSCP) and further confirmed by sequencing. Results 18 different mutations and 17 polymorphisms have been found, in which 7 mutations and 5 polymorphisms are novel, respectivelyOf them, Arg778Leu and Thr935Met reaching a mutation frequency of 37.7% and 10% of in WD chromosomes may be the hotspots of mutation in Chinese population.Ile1148Thr, previously defined as a possible disease-causing mutation may be a polymorphism which has not yet been detected in normal chromosomes. Conclusion In Chinese, WD seems resulting from a few relatively common and a large number more rare mutations

Objective To evaluate the effect of total parenteral nutrition (TPN) on hyperemesis gravidarum (HG) and pregnancy ending.Methods 27 cases of HG were treated with TPN and 30 cases with the routine infusion to adjust water electrolyte and acid base balance for the purpose of observation of vomiting stopping,time of turning ketonic negative and pregnancy ending.Results After 3 day treatment with TPN,stopping vomiting rate was 55.6%,time of turning ketonic negative was (3.1?0.84) days and neonatal weight was (3.4?0.38) kg,which was 13.3%,(5.2? 1.06) days and (2.9?0.45) kg respectively in control group.There was significant difference between the two groups (P

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the ovary in rats. Methods Forty female SD rats in normal estrous cycles were randomly divided into PBS group and NDGA group, subcutaneously injected of PBS solution and 3% NDGA at the dose of 30 mg/kg respectively every other day for 16 d. The serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E 2) and progesterone (P) were measured by chemiluminoimmunoassay before and after administration and the mating was confirmed by the occurrence of vaginal plugs and the morphological changes of uterus and ovaries were examined by immunohistochemistry after administration. Results None of the NDGA-treated rats were able to successfully mate as the absence of vaginal plugs were detected. The endometrium could maturate in all of the NDGA-treated rats, but there had a decrease not only in the number of endometrial glands, but also the proliferation rate of the glandular epithelial cells, the endometrial stroma cells, the vessels and the microvacular density (P

Objective To investigate the influence of FBG2 on the growth, proliferation, apoptosis, infiltration and cell cycle of the gastric cancer line MKN45. Methods A critical component ubiquitin-protein ligase complex, FBG2 cDNA, was subcloned into a constitutive vector pcDNA3.1, followed by transfection in MKN45 by using liposome. Then stable expression clones were selected and appraised. The apoptosis and cell cycles were detected using flow cytometry. The growth and proliferation were analyzed by plotting cell growth curves and colony formation assay respectively. The ability of infiltration was tested using cancer cell migration assay. The MKN-FBG2 group and two control groups were included in the study. Results MKN-FBG2 grew faster than MKN45 and MKN-PC. The cell counts of MKN-FBG2 on the fourth, fifth, sixth and seventh day were significantly larger than that of others (P

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the embryos growth and decidual development in rats. Methods Fifty pregnant SD rats were randomly divided into NDGA-treated group(N)and control group (P), which were given subcutaneous injection of NDGA or PBS on embryonic day 1(E1), E7 and E14, and then killed on E6 and E9. The number of implantations was calculated, the diameter of the embryos was measured and the numbers and weight of neonates were recorded. The expressions of VEGF, PCNA and FⅧRAg in the deciduas and embryos were detected by immunohistochemistry and then made image analysis. The serum concentrations of progesterone on E9 were measured. Results The number of implantations were decreased with NDGA treatment on E1 (P