This paper summarizes the recent 6～7 year advances in the research of pharmacological effects of polyactin including mainly six aspects: ①Effects of polyactin on functions of macrophages in vitro ; ② Mechanism enhancive effect ofpolyactin on NK cell activity; ③Effects of polyactin on the human lymphocyte immunofunction; ④Effects of polyactin on the proliferation and cytotoxicity of LAK; ⑤Enhances in specific antitumor activity in mice in vivo ; ⑥Effects of polyactin on erythrocyte deformability and immunofunction in Rabbit and Rat.

Objective To study the clinical effect of manipulation in the treatment of cervical vertigo due to atlantoaxial joint disorder. Methods A total of 86 patients in our hospital from year of July, 2010 to 2014, with cervical vertigo due to atlantoaxial joint disorder were divided into a control group and a treatment group randomly, with 43 patients in each group. The control group was treated by intravenous dripping of betahistine hydrochloride sodium chloride injection, while the treatment group was additionally treated by manipulation on the basis of the control group. The efficacy and symptoms changes together with average blood flow rate of LVA, RVA, BA, and the value of ADI, VBLADI, and DO were compared between the two groups after one week of the treatment. Results After the treatment, the improvement of vertigo and symptoms of the treatment group was between than the control group(t=12.655, P<0.05); the value of ADI and VBLADI decreased lower in the treatment group than the control group (t were 2.888 and 5.334 respectively, P<0.05);Vm of RVA, LVA and BA increased higher in the treatment group than the control group (t were 4.710, 3.534 and 5.335 respectively, P<0.01). Conclusions Manipulation combined with intravenous dripping of betahistine hydrochloride sodium chloride injection is effective in improving vertigo, decreasing ADI and VBLADI, and correcting atlantoaxial joint disorder.

Tachycardia-induced cardiomyopathy (TIC) is defined as a kind of cardiomyopathy with cardiac dilatation and dysfunction secondary to sustained or recurrent tachyarrhythmia.Myocardial dysfunction can wholly or partially recover after control of the responsible tachyarrhythmia.TIC,a reversible acquired cardiomyopathy with generally benign prognosis,can occur at any age;however,it often proves to be unrecognized by most of pediatricians in clinical practices.Now,the clinical criteria,pathogenesis,characteristics,therapy and prognosis of pediatric TIC were summarized,so as to provide a clinical basis for early recognition and prompt therapy.

Objective To evaluate the clinical effects of sinus tarsi approach and eight-shape approach in the treatment of calcaneal fractures.Methods Analyzed the outcomes of 23 patients(21 males and 2 females)with calcaneal fractures(25 feet)treated with plate via sinus tarsi approach and eight-shape approach.The mean age of patients was 38 years old(ranged from 24 to 55 years).According to Sanders classification:16 feet in type Ⅱ,9 feet in type Ⅲ.Results The average operation time was 85 min(ranged from 70 min to 100 min),and no wound compli-cations occurred.X-ray review showed that bony healing,and healing time was 3-6 months.The average duration of follow-up was 13 months(ranged from 12 to 18 months).The result was excellent in sixthteen cases,good in six cases and fair in one case according to Maryland score.The satisfactory rate was 95 .6%.Conclusion Open reduction and internal fixation via the sinus tarsi approach and eight -shape approach provides not only reliable fixation,but also less complications.

Objective To study and report 7 novel mutations and 5 novel polymorphisms of Wilson disease (WD) geneIn combination with the mutations and polymorphisms previously reported, mutation characteristics of WD gene in Chinese were further analysed. Methods Genomic DNA of 60 normal controls and 84 WD patients from 64 families were extracted from peripheral blood leukocytesThe mutations of WD gene (exon1～21) in these subjects were screened by PCR-single strand conformation polymorphism (SSCP) and further confirmed by sequencing. Results 18 different mutations and 17 polymorphisms have been found, in which 7 mutations and 5 polymorphisms are novel, respectivelyOf them, Arg778Leu and Thr935Met reaching a mutation frequency of 37.7% and 10% of in WD chromosomes may be the hotspots of mutation in Chinese population.Ile1148Thr, previously defined as a possible disease-causing mutation may be a polymorphism which has not yet been detected in normal chromosomes. Conclusion In Chinese, WD seems resulting from a few relatively common and a large number more rare mutations

Objective To evaluate the effect of total parenteral nutrition (TPN) on hyperemesis gravidarum (HG) and pregnancy ending.Methods 27 cases of HG were treated with TPN and 30 cases with the routine infusion to adjust water electrolyte and acid base balance for the purpose of observation of vomiting stopping,time of turning ketonic negative and pregnancy ending.Results After 3 day treatment with TPN,stopping vomiting rate was 55.6%,time of turning ketonic negative was (3.1?0.84) days and neonatal weight was (3.4?0.38) kg,which was 13.3%,(5.2? 1.06) days and (2.9?0.45) kg respectively in control group.There was significant difference between the two groups (P

0.05). But the CaO2-CjO2 and jugular vein Glu level at D in group I were significantly lower than those at D in group C. In group C CaO2-CjO2 and jugular vein glutamate level at D were significantly higher than those at B (P

Objective To study the IgG antibodies on red cell surface in ABO and Rh-incompatible infants and to evaluate its predictive value for hemolysis. Methods From Nov. 2001 to Jul. 2002,50 full term newborn babies with ABO and Rh incompatibility hemolysis were admitted .They were divided into two groups according to the direct Coombs test(DCT): 38 cases with positive result and 12 cases were negative. Another 30 normal newborns were chosen as control group. Erythrocyte associated IgG,A and M were measured by a flow cytometry (FCM). Results the mean level of concentration of IgG antibodies in positive DCT group was (12.64?16.83)%,while (4.54?2.54)% in negative DCT group ( P 427.5 ?mol/L,IgG was elevated with the increased TSB level. There was a close relationship between the concentration of TSB and IgG ( r =0.678). Conclusion Measurement of erythrocyte surface antigen IgG assists the diagnosis of maternal-fetal incompatible hemolysis,especially when with a positive direct Coombs test result.

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the ovary in rats. Methods Forty female SD rats in normal estrous cycles were randomly divided into PBS group and NDGA group, subcutaneously injected of PBS solution and 3% NDGA at the dose of 30 mg/kg respectively every other day for 16 d. The serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E 2) and progesterone (P) were measured by chemiluminoimmunoassay before and after administration and the mating was confirmed by the occurrence of vaginal plugs and the morphological changes of uterus and ovaries were examined by immunohistochemistry after administration. Results None of the NDGA-treated rats were able to successfully mate as the absence of vaginal plugs were detected. The endometrium could maturate in all of the NDGA-treated rats, but there had a decrease not only in the number of endometrial glands, but also the proliferation rate of the glandular epithelial cells, the endometrial stroma cells, the vessels and the microvacular density (P

Objective To investigate the effects of altered deceleration capacity of heart rate (DC) and heart rate deceleration runs(DRs) on the short-term prognosis of patients with acute myocardial infarction (AMI) .Methods 115 patients confirmed with AMI within 7 days as AMI group ,50 persons without MI were selected as control group .The deceleration capacity of heart rate and heart rate deceleration runs were detected by the 24 hours Holter within 7 to 14 after onset of AMI ,all the patients were divided in-to high risk group ,medium risk group and low risk group through the Holter results .Left ventricular ejection fraction (LVEF) was evaluated by echocardiography .Compare the risk of the AMI group with the control group .Meanwhile the AMI patients were fol-lowed up for mean(9 .2 ± 1 .7)months .Observe the presence of major adverse cardiovascular events (MACE) .All AMI patients were classified into MACE and non-MACE groups according to the presence of MACE .Analysis the risk factors of MACE .Results AMI group had a significant increase risk but decrease in deceleration capacity of heart rate (P<0 .05) as compared with the control group .In MACE group ,the rate of high risk was significant increasing(P=0 .005) ,but the rate of low risk was significant decrea-sing(P=0 .039) .The Logistic regression analysis showed that LVEF ,high risk group ,age and percutaneous transluminal coronary intervention(PCI) were independent risk factors on presence of MACE .Correlation r was 0 .33 ,0 .30 ,0 .23 ,0 .18 respectively ,and odds ratio was 4 .83 ,4 .28 ,2 .22 ,1 .58 respectively ,the correlation with deceleration capacity of heart rate was a little weaker than LVEF .The coefficient of determination r2 was 0 .528 when the LVEF and ages went into regression ,whereas that was 0 .635 when the LVEF ,high risk group and age went into regression together .Conclusion Capacity of heart rate decelerate was significantly weakened in AMI patients .LVEF ,the significantly decreased deceleration capacity of heart rate ,age and PCI were independent risk factors on presence of short-term MACE in AMI patients .The prognostic value of the DC and DRs was a little weaker than the LVEF .The better predictive value was obtained if the LVEF ,the significantly decreased deceleration capacity of heart rate ,age were combined .