This paper summarizes the recent 6～7 year advances in the research of pharmacological effects of polyactin including mainly six aspects: ①Effects of polyactin on functions of macrophages in vitro ; ② Mechanism enhancive effect ofpolyactin on NK cell activity; ③Effects of polyactin on the human lymphocyte immunofunction; ④Effects of polyactin on the proliferation and cytotoxicity of LAK; ⑤Enhances in specific antitumor activity in mice in vivo ; ⑥Effects of polyactin on erythrocyte deformability and immunofunction in Rabbit and Rat.

Tachycardia-induced cardiomyopathy (TIC) is defined as a kind of cardiomyopathy with cardiac dilatation and dysfunction secondary to sustained or recurrent tachyarrhythmia.Myocardial dysfunction can wholly or partially recover after control of the responsible tachyarrhythmia.TIC,a reversible acquired cardiomyopathy with generally benign prognosis,can occur at any age;however,it often proves to be unrecognized by most of pediatricians in clinical practices.Now,the clinical criteria,pathogenesis,characteristics,therapy and prognosis of pediatric TIC were summarized,so as to provide a clinical basis for early recognition and prompt therapy.

Objective To investigate the influence of FBG2 on the growth, proliferation, apoptosis, infiltration and cell cycle of the gastric cancer line MKN45. Methods A critical component ubiquitin-protein ligase complex, FBG2 cDNA, was subcloned into a constitutive vector pcDNA3.1, followed by transfection in MKN45 by using liposome. Then stable expression clones were selected and appraised. The apoptosis and cell cycles were detected using flow cytometry. The growth and proliferation were analyzed by plotting cell growth curves and colony formation assay respectively. The ability of infiltration was tested using cancer cell migration assay. The MKN-FBG2 group and two control groups were included in the study. Results MKN-FBG2 grew faster than MKN45 and MKN-PC. The cell counts of MKN-FBG2 on the fourth, fifth, sixth and seventh day were significantly larger than that of others (P

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the embryos growth and decidual development in rats. Methods Fifty pregnant SD rats were randomly divided into NDGA-treated group(N)and control group (P), which were given subcutaneous injection of NDGA or PBS on embryonic day 1(E1), E7 and E14, and then killed on E6 and E9. The number of implantations was calculated, the diameter of the embryos was measured and the numbers and weight of neonates were recorded. The expressions of VEGF, PCNA and FⅧRAg in the deciduas and embryos were detected by immunohistochemistry and then made image analysis. The serum concentrations of progesterone on E9 were measured. Results The number of implantations were decreased with NDGA treatment on E1 (P

Objective To investigate the effects of nordihydroguaiaretic acid(NDGA)on the embryos growth and decidualization of rat by intrauterine perfusion and to investigate the new medication for planned parenthood.Methods Fifty-six pregnant SD rats were randomly divided into NDGA-treated group(N)and PBS-treated control group(P)and blank control group(B).By intubation,NDGA or PBS was perfused into the uterus on embryonic day 1(E1)and day 7(E7).Rats were killed on E9 to calculate the number and measure the diameter of the embryos,as well as the number and weight of litters.The expression of VEGF,PCNA and FⅧRAg in deciduas and placenta were detected by immunohistochemistry and image analysis.The serum concentration of progesterone on E9 was measured.Results In comparison with two control groups,the number and the diameter of embryos after perfusion were decreased with NDGA treatment on E1(P0.05).Conclusion NDGA can inhibit the growth of early embryo and interfere with the progress of decidualization obviously by intrauterine perfusion,but has little effect on the serum concentration of progesterone.

Objective:To evaluate the effectiveness on the prevention of behavior problems of life skills education combining school-based and parent-involved approaches for third-grade students in China.Methods:This research was targeted at the population of third-grade children in two elementary schools in Qinhuangdao City,Hebei Province.Nine regular school classrooms were randomly divided into three groups:the intervention group(n=208),internal control group(n=209) and external control group(n=204).The intervention included 26-hour competence promotion for students and 5-hour parent training.The Rutter Scale by parent and teacher were used to evaluate the effects at pretest,posttest and 6-month follow-up.Results:Improvement was observed among children in the intervention group than those in the control groups.The statistical difference was significant(P

Objective To study and report 7 novel mutations and 5 novel polymorphisms of Wilson disease (WD) geneIn combination with the mutations and polymorphisms previously reported, mutation characteristics of WD gene in Chinese were further analysed. Methods Genomic DNA of 60 normal controls and 84 WD patients from 64 families were extracted from peripheral blood leukocytesThe mutations of WD gene (exon1～21) in these subjects were screened by PCR-single strand conformation polymorphism (SSCP) and further confirmed by sequencing. Results 18 different mutations and 17 polymorphisms have been found, in which 7 mutations and 5 polymorphisms are novel, respectivelyOf them, Arg778Leu and Thr935Met reaching a mutation frequency of 37.7% and 10% of in WD chromosomes may be the hotspots of mutation in Chinese population.Ile1148Thr, previously defined as a possible disease-causing mutation may be a polymorphism which has not yet been detected in normal chromosomes. Conclusion In Chinese, WD seems resulting from a few relatively common and a large number more rare mutations

Objective To evaluate the effect of total parenteral nutrition (TPN) on hyperemesis gravidarum (HG) and pregnancy ending.Methods 27 cases of HG were treated with TPN and 30 cases with the routine infusion to adjust water electrolyte and acid base balance for the purpose of observation of vomiting stopping,time of turning ketonic negative and pregnancy ending.Results After 3 day treatment with TPN,stopping vomiting rate was 55.6%,time of turning ketonic negative was (3.1?0.84) days and neonatal weight was (3.4?0.38) kg,which was 13.3%,(5.2? 1.06) days and (2.9?0.45) kg respectively in control group.There was significant difference between the two groups (P

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the ovary in rats. Methods Forty female SD rats in normal estrous cycles were randomly divided into PBS group and NDGA group, subcutaneously injected of PBS solution and 3% NDGA at the dose of 30 mg/kg respectively every other day for 16 d. The serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E 2) and progesterone (P) were measured by chemiluminoimmunoassay before and after administration and the mating was confirmed by the occurrence of vaginal plugs and the morphological changes of uterus and ovaries were examined by immunohistochemistry after administration. Results None of the NDGA-treated rats were able to successfully mate as the absence of vaginal plugs were detected. The endometrium could maturate in all of the NDGA-treated rats, but there had a decrease not only in the number of endometrial glands, but also the proliferation rate of the glandular epithelial cells, the endometrial stroma cells, the vessels and the microvacular density (P

Objective To investigate the application v al ue of stereotactic vacuum-assisted core needle breast biopsy (Mammotome biopsy) for breast masses and the surgical treatment for atypical ductal hyperplasia (AD H) detected by the Mammotome biopsy. Methods Ultrasound guide d Mammotome biopsies and corresponding surgical management were carried out in 3 2 patients (39 lesions) in this hospital between March 2003 and January 2004. Results Of the 32 patients (39 lesions), fibroadenosis was diag nosed in 24 patients (31 lesions), plasma cell mastitis in 1 patient, atypical d uctal hyperplasia in 4 patients, and breast cancer, 3 patients. Of the 4 patient s with atypical hyperplasia, a re-operation was conducted and a confirmative dia gnosis of atypical hyperplasia was made in 2 patients, while the oral administra tion of Tamoxifen was given in 1 patient with mild atypical hyperplasia and in 1 patient with moderate to severe hyperplasia. Conclusions For patients with severe ADH detected by the Mammotome biopsy, a re-operation is req uired. For patients with mild to moderate ADH: if the breast mass is palpable pr eoperatively, a surgical excision is recommended; if the patient has non-palpabl e masses with negative family history, in condition that the lesion has been ent irely removed, the surgery is not necessary and the oral administration of Tamox ifen with regular follow-up is indicated.