This paper summarizes the recent 6～7 year advances in the research of pharmacological effects of polyactin including mainly six aspects: ①Effects of polyactin on functions of macrophages in vitro ; ② Mechanism enhancive effect ofpolyactin on NK cell activity; ③Effects of polyactin on the human lymphocyte immunofunction; ④Effects of polyactin on the proliferation and cytotoxicity of LAK; ⑤Enhances in specific antitumor activity in mice in vivo ; ⑥Effects of polyactin on erythrocyte deformability and immunofunction in Rabbit and Rat.

Objective:To evaluate the effectiveness on the prevention of behavior problems of life skills education combining school-based and parent-involved approaches for third-grade students in China.Methods:This research was targeted at the population of third-grade children in two elementary schools in Qinhuangdao City,Hebei Province.Nine regular school classrooms were randomly divided into three groups:the intervention group(n=208),internal control group(n=209) and external control group(n=204).The intervention included 26-hour competence promotion for students and 5-hour parent training.The Rutter Scale by parent and teacher were used to evaluate the effects at pretest,posttest and 6-month follow-up.Results:Improvement was observed among children in the intervention group than those in the control groups.The statistical difference was significant(P

Tachycardia-induced cardiomyopathy (TIC) is defined as a kind of cardiomyopathy with cardiac dilatation and dysfunction secondary to sustained or recurrent tachyarrhythmia.Myocardial dysfunction can wholly or partially recover after control of the responsible tachyarrhythmia.TIC,a reversible acquired cardiomyopathy with generally benign prognosis,can occur at any age;however,it often proves to be unrecognized by most of pediatricians in clinical practices.Now,the clinical criteria,pathogenesis,characteristics,therapy and prognosis of pediatric TIC were summarized,so as to provide a clinical basis for early recognition and prompt therapy.

0.05). But the CaO2-CjO2 and jugular vein Glu level at D in group I were significantly lower than those at D in group C. In group C CaO2-CjO2 and jugular vein glutamate level at D were significantly higher than those at B (P

Objective To evaluate the efficacy of video-assisted thoracoscopic surgery(VATS)for spontaneous pneumothorax.Methods From January 2003 to June 2006,VATS was carried out in 61 cases of spontaneous pneumothorax in our hospital.Resection or ligatation of pulmonary bullae,and pleurodesis were performed by using Endo-GIA.Results Among the cases,unilateral VATS was performed on 58 patients(an axillary small incision was made in one case),and bilateral VATS was done in 3.No conversion to open surgery or severe postoperative complications occurred in this series.Pulmonary bullae was detected in 56 of all the patients.The operation time was 35-55 min(mean,46 min)for unilateral VATS,and was 85,175,and 190 min respectively for the 3 cases of bilateral VATS.The mean blood loss for unilateral operation was 40 ml(range,30-45 ml),and the blood loss in the 3 cases of bilateral VATS was 55,60,and 200 ml.The thoracic-drainage volume was 230-500 ml(mean,390 ml)in the unilateral cases,and was 350,1030,and 1200 respectively in the 3 bilateral cases.The drainage tube was withdrawn 3-6 d(mean 4 d)later in the unilateral cases,and 3,4,and 5 d later in the 3 bilateral cases.The 61 patients were followed up for 4-24 mon(mean,8 mon),during which one patient experienced recurrent pneumothorax in 2 months and was cured by closed thoracic drainage.Conclusions VATS is safe and effective for patients with spontaneous pneumothorax.

BACKGROUND:Gene therapy is the direction of spinal cord injury(SCI) therapy,the key of which is construction of targeting gene and vector. OBJECTIVE:To construct the recombinant adenovirus vector carrying human brain-derived neurotrophic factor(hBDNF) marked enhanced green fluorescent protein(EGFP). DESIGN,TIME AND SETTING:A single sample observation was completed in the First Affiliated Hospital of Fujian Medical University from September 2007 to June 2008. MATERIALS:Competent E. coli DH-5? was obtained from the American Stratagene Company. Plasmid pDC316-hBDNF,pDC316-mCMV-EGFP,pBHGlox_E1,3Cre and package system AdMax and 293 package cell strain were purchased from the Canadian Mixcrobix-Biosystems Company. METHODS:The hBDNF gene was constructed by PCR with plasmid pDC316-BDNF as template. With enzyme digestion,the hBDNF gene was inserted into the vector pDC316-mCMV-EGFP and the shuttle plasmid pDC316-hBDNF-mCMV-EGFP was constructed,which was cotransfected with the adenovirus skeleton plasmid pBHGlox_E1,3Cre into 293 cells to obtain the produced replication defective recombinant adenovirus vector Ad-hBDNF-EGFP. The recombinant adenovirus was propagated by repeat infection of 293 cells and purified by ion exchange method,then the virus particles were counted and the purity and titer were determined. MAIN OUTCOME MEASURES:①PCR identification of plasmid pDC316-hBDNF. ②Construction and identification of the shuttle plasmid pDC316-hBDNF-mCMV-EGFP. ③Packing,amplification and purification of recombinant adenovirus vector Ad-hBDNF-EGFP. ④PCR identification of the recombinant adenovirus. ⑤Titer of recombinant adenovirus. RESULTS:PCR amplification,restriction analysis and sequencing identified that both recombinant shuttle plasmid pDC316-hBDNF-mCMV-EGFP and recombinant adenovirus vector Ad-hBDNF-EGFP were correctly constructed. After amplification and purification,the virus particle count,A260/A280 and titer of recombinant adenovirus were 2.4?1011 VP/mL,2.0 and 0.8?1010 CCID50/mL,respectively. CONCLUSION:Recombinant adenovirus vector Ad-hBDNF-EGFP is successfully constructed,which laid a foundation for further study regarding gene function and therapy.

Objective To evaluate the clinical value of laparoscopic,retroperitoneal laparoscopic,and open radical nephrectomy.Methods Clinical data of 32 cases of laparoscopic radical nephrectomy(Laparoscopic Group),18 cases of retroperitoneal laparoscopic radical nephrectomy(Retroperitoneal Group),and 32 cases of open radical nephrectomy(Open Group) were compared in respect of operation time,length of hospitalization after operation,intraoperative hemorrhage volume,and incidence of postoperative complications.Results With exception of 1 case of conversion to open surgery because of ruptured renal vein in the Laparoscopic Group,the operation was successfully completed in all the cases.Of the Laparoscopic Group,Retroperitoneal Group,and Open Group,the operation time was 100.5?19.2 min,90.3?21.4 min,and 127.2?20.5 min,respectively,the intraoperative hemorrhage volume was 40?15 ml,50?15 ml,and 200?30 ml,respectively,and the length of postoperative hospitalization was 5.2?1.3 d,5.6?1.1 d,and 9.1?1.8 d,respectively.Both the Laparoscopic Group and the Retroperitoneal Group were superior to the Open Group(P

130 mg/dl). Conclusions The fibrinogen level may reduce to 40～130 mg/dl treated with Batroxobin in patients with acute cerebral infarct, but whether it could be regarded as a target level need more studies.

Objective To investigate the effects of altered deceleration capacity of heart rate (DC) and heart rate deceleration runs(DRs) on the short-term prognosis of patients with acute myocardial infarction (AMI) .Methods 115 patients confirmed with AMI within 7 days as AMI group ,50 persons without MI were selected as control group .The deceleration capacity of heart rate and heart rate deceleration runs were detected by the 24 hours Holter within 7 to 14 after onset of AMI ,all the patients were divided in-to high risk group ,medium risk group and low risk group through the Holter results .Left ventricular ejection fraction (LVEF) was evaluated by echocardiography .Compare the risk of the AMI group with the control group .Meanwhile the AMI patients were fol-lowed up for mean(9 .2 ± 1 .7)months .Observe the presence of major adverse cardiovascular events (MACE) .All AMI patients were classified into MACE and non-MACE groups according to the presence of MACE .Analysis the risk factors of MACE .Results AMI group had a significant increase risk but decrease in deceleration capacity of heart rate (P<0 .05) as compared with the control group .In MACE group ,the rate of high risk was significant increasing(P=0 .005) ,but the rate of low risk was significant decrea-sing(P=0 .039) .The Logistic regression analysis showed that LVEF ,high risk group ,age and percutaneous transluminal coronary intervention(PCI) were independent risk factors on presence of MACE .Correlation r was 0 .33 ,0 .30 ,0 .23 ,0 .18 respectively ,and odds ratio was 4 .83 ,4 .28 ,2 .22 ,1 .58 respectively ,the correlation with deceleration capacity of heart rate was a little weaker than LVEF .The coefficient of determination r2 was 0 .528 when the LVEF and ages went into regression ,whereas that was 0 .635 when the LVEF ,high risk group and age went into regression together .Conclusion Capacity of heart rate decelerate was significantly weakened in AMI patients .LVEF ,the significantly decreased deceleration capacity of heart rate ,age and PCI were independent risk factors on presence of short-term MACE in AMI patients .The prognostic value of the DC and DRs was a little weaker than the LVEF .The better predictive value was obtained if the LVEF ,the significantly decreased deceleration capacity of heart rate ,age were combined .