Objective:Heart rate variability (HRV) contains a lot of information concerning the cardiovascular regulation,and can be an important indicator,quantitatively reflecting of autonomic nervous function and the normal heart activity,also can play an important role in cardiovascular regulation.Therefore,HRV signals are analyzed using common HRV analysis methods.Methods:Decompose HRV signals of congestive heart failure and normal persons into 6 scales using Multi-resolution analysis.The wavelet coefficient energy is calculated,especially the frequency energy of cd3 to cd6.Results:Energy is mainly concerned in the lower band of high frequency band and low frequency band,where the energy differences between the two groups are more evidence.It's more precise compared to the result with frequency-domain analysis.Conclusions:To gain results with universal law,more different analysis methods need to be improved and lots of researches are necessary.

Five hundred elderly patients with ischemic stroke admitted from January 2008 to December 2010 were enrolled in the study,including 352 recurrent cases and 148 primary cases.There was a positive correlation of stroke recurrence with age,blood pressure,blood glucose and lipid levels,smoking and alcohol consumption of patients(P ＜0.05 ); while the stroke recurrence was negatively correlated with physical exercise(P ＜ 0.05).Stepwise multiple regression analysis indicated that the blood pressure,blood glucose and lipid levels were risk factors for the recurrence of ischemic stroke in elderly patients.

The injury of peripheral nerve is generally accompanied with active regeneration responses. This paper is to summarize the molecular mechanism to promote the nerve regeneration, including various reactions of the neuronal body, nerve fiber, and regulatory molecules in the microenvironment, such as transcription factors, inflammatory mediators, nerve growth factors, etc., aiming to investigate the possible mechanism in the nerve regeneration after injury.

A new fusion expression vector, pED-P8-Hi-lys was designed and constructed. It includes four parts, a 20 peptide sequence of hirudin that can maintain anticoagulant activity, the C-terminus of asparaginase as a fusion partner, basic octopeptide (KRKRKKSR) that makes the fusion partner easy to remove, and the unique acid-labile aspartyl-prolyl bond. It was transformed into E. coli BL-21 and the fusion protein (AnsB-C-P8-Hi-lys) was expressed effectively as inclusion bodies after inducing by lactose. The objective peptide Hi-lys was purified by means of cell disruption, washing, ethanol precipitation, acid hydrolysis, and DEAE-cellulose 52 column chromatography. The antithrombin activity of the purified Hi-lys peptide was about 50 ATU/mg by thrombin activity assays.

In recent years ,the diffusion magnetic resonance imaging in the study of breast cancer is in-creasing,which including diffusion weighted imaging ,diffusion tensor imaging ,intravoxel incoherent motion ,and diffusion kurtosis imaging .The emergence and development of these technologies can be noninvasive quantitative evaluation of breast tumors from the molecular level .The researchers pay more and more attention to the applica-tion of breast cancers diagnosis and differential diagnosis ,therapeutic effect ,prognosis evaluation and so on .This paper reviews the progresses of the above four technologies in the use of the breast cancer .

Purpose: To investigate the plasma levels of chemokine IL-8, GM-CSF, RANTES and MCP-1 in patients with breast carcinoma and in healthy controls and to explore its clinical, pathologic and diagnostic significance. Methods: Enzyme linked immunosorbent assay ( ELISA) kits were used in 93 breast cancer patients and 20 health controls, and clinical significance was analysed. Results: The plasma levels of IL-8, RANTES and MCP-1 were significantly higher in the patients than in

Objective This test is focused on functional magnetic resonance imaging(fMRI) of visual cortex by using echo planar imaging(EPI) and blood oxygenation level dependent(BOLD) contrast. Methods 36 normal volunteers and 17 patients with lesions at occipital lobe were included in this test. The echo planar images of the occipital area were acquired at visual stimulation and rest phases. Results The primary visual cortex was located at the bilateral calcarine cortex, which belonged to Brodmann area 17. The functional areas showed displacement due to the mass effect of lesions near calcarine cortex in 3 patients. Conclusion fMRI can be used to study human functional cortex. A preliminary location of visual cortex can be acquired through visual stimulation. The invasion of the intracranial massive lesions to functional areas the disappearance of functional activation. The mass effect of intracranial lesions may lead to deformation or displacement of the near functional areas.

OBJECTIVE To evaluate the antibacterial activity of Extractum Sophorae Flavescentis(ESF) against Staphylococcus epidermidis in vitro.METHODS Determination of antibacterial activity of ESF against 208 strains S.epidermidis was performed by using agar dilution method.RESULTS The antibacterial efficiency of ESF against 208 strains of S.epidermidis was good,and the MIC90 of that against 110 strains of MRSE and 98 strains of MSSE were 1185 ?g/ml and 0.286 ?g/ml,respectively.CONCLUSIONS Extractum Sophorae Flavescentis has strong antibacterial activity against 208 strains of S.epidermidis.

Objective To establish a method of isolating and purifying islets from Wistar rat, and to evaluate the biological characters of the isolated and purified islets. Methods The pancreases were surgically removed from adult Wistar rats, carefully minced with scissors as small as possible prior to incubation in 0.9 mg/ml collagenase (Sigma, type Ⅴ) for 10~17min at 37℃. The digested tissue was filtered through a 60 apertures/cm~2 metal sieve and washed with 10% fetal bovine sera (FBS) in Hank’s solution. Purification was performed using Dextran discontinuous density gradient centrifugation to separate the contents of the postcollagenase pellets. Islet samples were stained with DTZ staining for identification. The viability of the purified islet was determined by acridine orange/propidium iodide (AO/PI) double staining. The in vitro function was assayed by different densities of glucose incubating islets for 45min, respectively. Insulin secretion in the supernatant was assayed by radioimmunoassay. Islets were transplanted under the kidney capsule into recipient SD rats that had been rendered diabetic by injecting streptozotocin 50mg/kg, so as to evaluate the in vivo function of islets. Results The islets were recovered between the 11% and 22% Dextran layers, and between the 11% and 1640 layers. The average number of isolated islets was 2181?14 per rat pancreas. After a discontinuous Dextran density gradient purification, about 1826?24 islets were obtained from per rat pancreas, with an average purity over 95%. Islets showed good response to glucose and high efficiency to invert hyperglycemia 7-10d after transplantation. Conclusion These results showed that the digestion methods and purification procedure which we have developed could provide islets from rats for transplantation studies. The biological characters of islets harvested by our method were very well.

Objective To explore the effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of β-catenin in human colorectal cancer cells HT-29.Methods The recombinant plasmids were transfected into HT-29 cells mediated by lipofectamine~(TM) 2000, and detected by green fluorescence and RT-PCR. Western blot was applied to detect β-catenin expression level in HT-29 cells after transfection, and gray scales of electrophoresis strips were analyzed by SPSS 13.0.Results Green fluorescence and RT-PCR made clear that all 5 recombinant plasmids were successfully expressed in HT-29 cells. Western blot showed that β-catenin expression level in HT-29 cells was not affected after being transfected with pEGFP-N3-APC1, pEGFP-N3-APC2 and pEGFP-N3-APC3, and was distinctly affected after being transfected with pEGFP-N3-APC4 and pEGFP-N3-APC5, especially the later one. Conclusion The selected APC5 gene fragment with 15-amino acid repeats and SAMP repeats, which is relatively short, can degrade β-catenin level in HT-29 cells and may be applied in the gene therapy.