Objective　To establish a simple assay for quick scanning of effective agents in growth inhibition and apoptosis induction. Methods　After observing the correlation between cell number and acid phosphatase activity, and between cell apoptosis and acid phosphatase activity, we established a microplate densimetry method to measure the acid phosphatase activity. We also compared the sensitivity with MTT assay and confirmed cell apoptosis by flow cytometry. Results　In two cell lines of cancer (Hep G2 and CBRH-7919 cells), the acid phosphatase activity was correlated well with the cell number, and was directly proportional to the cell number in the range of 0.5×103～0.7×103. The coefficient reached to 0.994. After stimulation of phorbel ester (TPA) for one hour, the acid phosphatase activity rose significantly. In contrast, after the inhibition of cell growth by As2O3, the acid phosphatase activity decreased significantly. The higher the concentration of As2O3, the lower the acid phophatase activity. The acid phophatase activity went down significantly when apoptosis in Hep G2 cells was only 3.98% and no apoptosis in CBRH-7919 cells after 24-hour treatment with As2O3. Conclusions　The acid phophatase activity of cell may be used to measure cell proliferation and apoptosis. Because of the easiness and quickness, acid phophatase assay might be an ideal approach to scan the effective growth inhibitors and apoptosis inducers in a large number of drugs.

AIM: To find out characteristic UV spectum of Isatis Root Granules for identification. METHODS:Spectral correlation coefficient was calculated and compared between Isatis Root Granules and Radix Isatidis. Correl function from the Ms-Excel were applied for spectral calculation of three commodities. RESULTS: Spectral correlation coefficient was good between Isatis Root Ganules and Radix Isatidis. CONCLUSION: Fingerprint of Isatis Root can identificate whether granules contained isatis or not and its content.

Currently,research and practice in the of hospital management in China are at low level.This paper agrees that the innovation of hospital research management should be based on scientific knowledge management,knowledge communities is a core component of knowledge management implementation in hospitals.This paper descripts the method that how to buide the knowledge community,for example,the types of knowledge community,hospital knowledge system,and the job title based knowledge map and process-oriented knowledge map.

Objective: To investigate the application of conventional ultrasound and 16-slice CT examination in clinical diagnosis of hepatocellular carcinoma (HCC) and hepatic focal nodular hyperplasia. Methods: To choose 94 cases patients were receiving treatment in our hospital who were diagnosed as hepatic focal lesion through percutaneous biopsy of hepatocyte or histopathological examination of postoperation, and then take conventional ultrasound and 16-slice CT, respectively, to detect them. To compare and analyze the two methods in series of indicator, such as accuracy, the number of lesion size and the imageology character. Results: Conventional ultrasound diagnosis of benign tumor lesions was consistent with spiral CT results, and the difference was not statistical significance (x2=0.5556, P>0.05). The accuracy of spiral CT for hepatic carcinoma was higher than that of conventional ultrasound, and the difference was significant (x2=8.1987, P<0.05). The consistent rate of the spiral CT group with real result for micro-miniature carcinoma (the diameter less than 1 cm) was higher than that of conventional ultrasound group, and their difference was significant (x2=14.4028, P<0.05). The disappear and persist time of hepatic carcinoma patients were longer than that of non-hepatic carcinoma patients, and the difference between them was significant (t=9.7531, P<0.05). Conclusion: It is higher accuracy that 16-spiral CT can diagnose malignancy lesions in earlier stage.

Midwifery competency is the foundation for sustainable development of the midwifery profession. The promotion of midwives′ competency can improve their ability, strengthen midwifery competitiveness and enhance the quality of midwifery care services. This study gave an overview of the worldwide research progress on midwifery competency, we can draw the conclusion that there are urgent needs to improve midwifery education programs in China, emphasize competency-based midwifery education, as well as standardize the in-service training system to support the development of midwifery competency.

Objeract:To evaluate the effects of controlled release tinidazole(TNZ) membrane in the treatment of adult periodontitis. Methods:Adult periodontitis was treated with TNZ membrane in 15 cases (25 teeth),with Metronidazole(MNZ) in 15 cases (24 teeth) and with vehicle membrane in 15 cases (25 teeth).The effects were evaluated by clinical observation,biochemical examination and becteria test. Results: TNZ membrane more significantly inhibited bacteria (P

Acute Disease ; Adolescent ; Adult ; Bridged-Ring Compounds ; poisoning ; Cardiomyopathies ; etiology ; therapy ; Creatine Kinase ; blood ; Creatine Kinase, MB Form ; Electrocardiography ; Foodborne Diseases ; blood ; complications ; therapy ; Glutamyl Aminopeptidase ; blood ; Humans ; Isoenzymes ; blood ; Male ; Middle Aged ; Nausea ; etiology ; Seizures ; etiology ; Treatment Outcome

Objective To evaluate MR imaging of peripheral nerve tumors of the lower extremity. Methods MR imaging of peripheral nerve tumors of the lower extremity in five cases proved by surgery and pathology were retrospectively reviewed. In all patients, routine scanning was performed in axial, coronal, and sagittal planes, including T 1-weighted and T 2-weighted images. In two patients (schwannomas), T 1WI were obtained following intravenous injection of Gd-DTPA. Results There were four schwannomas (three benign and one malignant) and one malignant neurofibroma. Tumors arose at the following sites: leg ( n =2), popliteal region ( n =1), thigh ( n =1), and femoral region ( n =1). On T 1WI, tumors generally showed isointensity (two benign schwannomas) or lower-medium signal intensity to adjacent muscle with minimal inhomogeneity (one benign schwannoma, one malignant schwannoma, and one malignant neurofibroma). On T 2WI, tumors demonstrated inhomogeneous high signal intensity in all five patients. The target sign with peripheral hyperintense rim and central low intensity was see in two benign schwannomas on T 2WI. Conclusion MRI is useful in defining the location and extent of a lesion and in assisting the surgical planning. The target pattern appears to be a useful sign in the diagnosis of peripheral nerve tumors.

Objective To evaluate MR imaging of transient osteoporosis.Methods MR imaging of transient osteoporosis in eight patients was retrospectively reviewed.In all eight patients,routine scanning was performed in axial and coronal planes,including T_1-weighted and T_2-wighted images.Of the eight patients,five were male and three were female,with the age ranging from 12 to 70 years.Neither of the women was pregnant when they visited our hospital.Results The bilateral hips were affected in seven patients,the left shoulder in one.The MR images demonstrated low signal intensity in all eight patients on T_1WI,and normal signal intensity (2 cases),medium-high signal intensity (3 cases),or high signal intensity (3 cases) on T_2WI.The bone marrow edema (BME) pattern involved the acetabulum (one hip),both the femoral head in 5 hips,the femoral neck and the intertrochanteric region through the upper femur in 7 hips,and the upper humerus in one.A small joint effusion was observed in six hips on T_2WI.Conclusion MRI is useful in defining the location and extent of transient osteoporosis.

Objective To explore the synergistic effectiveness of hyperthermia and chemotherapy in the treatment of advanced gastric cancer.Methods Eighty-nine patients with advanced gastric cancer were randomly assigned to a study group which received a CapeOx chemotherapy regimen supplemented with hyperthermia or to a control group which received only the CapeOx regimen.The regimen consisted of capecitabine (1000 mg/m2,bid,orally for 14 consecutive days) plus oxalipaltin (130 mg/m2) on day 1.The hyperthermia was at 43℃ for 60 min in the tumor area on day 1 and twice a week thereafter.One cycle was 21 days.After 2 treatment cycles,efficacy was evaluated according to RECIST standards,improvements in the quality of life were assessed according to Karnofsky's performance status (KPS) and the side-effects of therapy were recorded.Results The response rate was 68.9％ in the study group and 36.4％ in the control group,showing a significant difference between the groups after two treatment cycles.The median progress-free survival (PFS) was 8.3 months in the study group vs 5.2 months for the controls.The 1-year survival rate was 66.4％ vs 45.5％ and the rate of improvement in KPS was 77.8％ vs 45.5％.All these differences were statistically significant.The common adverse effects were gastrointestinal toxicity,marrow depression and peripheral nerve abnormalities,but these adverse effects were all mild and similar in the two groups.Conclusion Hyperthermia when combined with the CapeOx chemotherapy regimen might improve the therapeutic effect in advanced gastric cancer without obviously increasing the adverse effects.