Traditional Chinese medicine teaching in general medical university was unsatisfied under the influence of many problems such as indefinite purpose,few class hours but many teaching contents,the different theoretical system between traditional Chinese medicine and western medicine,and theory divorced from practice etc.Therefore,the author suggested several measures that may help increasing teaching quality such as making definite course purpose and stimulating students' learning interest,enhancing training of imagery thinking,carefully electing teaching contents,integrating theory with practice and increasing clinical practice etc.

Objective To explore medication rule of vertigo and headache of TCM master Yan Zhenghua.Methods In this study,44 prescriptions for vertigo and 32 prescriptions for headache were collected and analyzed based on ACCESS database.Results The three most commonly used herbs in prescription for vertigo are red peony root (100％),white peony root (79.5％),and gastrodia tuber (65.9％),and the most commonly used two-drug compatibility is red peony root and gastrodia tuber (61.4％) ; the three most commonly used herbs in prescription for headache are red peony root (68.8％),tribulus terrestris (68.8％),and white chrysanthemums (65.6％),and the most commonly used two-drug compatibility is red peony root and tribulus terrestris (59.4％).Conclusion Professor Yah Zhenghua's basic governing method to treat vertigo and headache ispromote blood circulation and relieve painandpacify the liver to subdue yang.

Objective To compare postoperative outcomes and complications between subtotal colectomy combined with modified Duhamel procedure and simple subtotal colectomy for severe functional constipation (SFC). Methods Between January 2006 and June 2010,40 SFC patients after strict but inefficient nonoperative treatments were randomized by number table method to control group (20 cases, receiving simple subtotal colectomy) and combined group (20 cases,receiving subtotal colectomy and modified Duhamel procedure). The selection criteria were normal colonoscopy,and abnormal dynamic proctography (DPG). The functional outcomes after surgery were assessed from 6 months to 2 years period. Results The preoperative clinical manifestations of the two groups were similar. No statistically significant difference was observed between the two groups for time for recovery of the bowel function,length of postoperative hospitalization.and the early postoperative complications (P > 0.05). Functional outcomes of combined group with CIQOL score [(110.5 ±5.0) scores],relieving of costive gastrointestinal symptoms [(79.0 ±6.5)%], recurrent constipation rate [10.0% (2/20)], satisfaction rate of defecation frequency [85.0%(17/20)], satisfaction rate of quality of life[90.0%(18/20)] were significantly better than those of control group[(90.5 ± 3.5) scores, (60.5 ± 2.8)% ,35.0%(7/20), 15.0%(3/20),20.0%(4/20)](P< 0.05). Conclusion Combined application of subtotal colectomy and modified Duhamel procedure for SFC has better outcomes.

Objective To observe the clinical effects of treating Vetigo due to Vertebrobasilar Insufficient blood supply and hyperactivity of liver-Yang with Tianzhi Granules. Methods 118 cases were randomly recruited into a treatment group with 62 cases and a control group with 56 cases. On base of routine treatment, the treatment group was treated with Tianzhi Granules orally; and the control group was treated with Duflomed Hydrochloride orally. One course of treatment was 14 days. Results The total effective rates were 96.77%and 78.57% in treatment group and control group respectively. There was a significant difference between the two groups(P<0.05). The blood flow rat(BFR)of brain was improved in both groups, but the improvement of the BFR in brain was significantly better in the treatment group than the control group (P< 0.05). No adverse reaction was found in the treatment group. Conclusion Tianzhi Granules is effective and safe in treating Vetigo due vertebrobasilar insufficient blood supply.

Objective To investigate the effects of midazolam on extracellular signal-regulated kinase 1 (ERK1),ERK2 and cyclic AMP response element binding protein (CREB) phosphorylation in hippocampal in rats.Methods Eighty male SD rats weighing 250-300 g were randomly divided into 2 groups ( n = 40) : group control (group C) and group midazolam (group M).The animals underwent a continuous multi-trial inhibitory avoidance training .The times of trial needed for each animal to attain the learning criterion ( 100 s) were recorded.Each animal was given intraperitoneal midazolam 3 mg/kg or normal saline 2 ml/kg at 15 min before training.The memory retention was tested at 0.5,1,2 and 24 h (n = 8,at each time point)after the training session and the memory latency was recorded.The animals were sacrificed 15 min after administration (T0) and after the memory testing (T1-4) and hippocampns was obtained for determination of phosphorylated ERK1 (p-ERK1),p-ERK2 and p-CREB expression.Results Compared with group C,the times of trial to attain the learning criterion were significantly increased,memory latency shortened at T2-4,ERK1 phosphorylation decreased at T0,3.4 while ERK2 and CREB phosphorylation decreased at T0-4.Conclusion Midazolam can inhibit ERK1,ERK2 and CREB phosphorylation in hippocampal in rats.

In recent years, the concept of minimally invasive surgery has become accepted by the surgical community, though there are limitations in locating small gastrointestinal tumors when laparoscopy is used alone. Meanwhile, endoscopy is an excellent tool for locating these small tumors, though one must take extreme care to avoid hollow viscus perforation. Combination of laparoscopy and gastroduodenoscopy has extensive application in the resection of gastrointestinal stromal tumors, sessile gastric polyps and early gastrointestinal carcinoma. During laparoscopic operation, the endoscopist can help to locate the tumor or polyp. Endoscopy can also help to determine whether or not the tumor or polyp has been resected completely. In performing traditional laparoscopic colectomy for left-sided colonic tumors, specimen retrieval necessitates a mini-incision which is often the cause of postoperative pain, wound infection, and other pain-related complications. The combination of laparoscopy with transanal endoscopic microsurgery is feasible for selected patients with left-sided colonic tumors, and complications related to mini-incision can be avoided completely. Combination of laparoscopy and gastrointestinal endoscopy also benefits patients with acute bowel obstruction prior surgical operation. With the help of perineum-bowel tube, sigmoidoscopic technique can relieve acute bowel obstruction, so that these patients may have chance for laparoscopic operation. In summary, this hybrid approach can not only decrease surgical incisions, but also avoid some of the surgical risks of emergent operations.

For the purpose of creating a hospital culture, the present paper summarizes theories on research-oriented organizations, addresses the significance of developing a research-oriented hospital, summarizes the means and methods for developing a research-oriented hospital according to the model of a research-oriented organization, and demonstrates the effects of a research-oriented hospital and promoting functions for hospital culture.

Aim To study the regulating effect of Tongxinluo on HIF-1α,VEGF that Generated by the Human brain microvascular endothelial cells induced by β-amyloid 1-42.Methods Human brain microvascular endothelial cells were pre-incubated for 4h by Tongxinluo, then injured by β-amyloid 1-42.VEGF protein and HIF-1α were detected by Western blot.Result In the Aβ treated Human brain microvascular endothelial cells,viable cells decreased.The expression of VEGF was Reduced, and HIF-1α elevated. After the cells were treated with Tongxinluo, all the above indexes were improved.Conclusion Tongxinluo can enhance the VEGF protein expressions by the HIF-1α and protect the Human brain microvascular endothelial cells.

BACKGROUND: A considerable number of epilepsy patients cannot be treated sufficiently by drug. Epilepsy surgery is a treatment option in these cases. However, precisely localizing epileptogenic zone in epileptic patients is a successful element of epilepsy surgery. Its goal is to remove a minimum volume to control the seizures without cognitive impairment. Presurgical evaluation typically involves electroencephalogram (EEG), video-EEG monitoring, magnetic resonance imaging (MRI), single photon emission computed tomography and neuropsychological testing. Magnetoencephalography (MEG) has been as a noninvasive technique to be used to epilepsy surgical planning and brain functional study in many countries.OBJECTIVE: To preoperatively localize epileptogenic zone in patients with lesion-associated epilepsy using magnetoencephalography, compare with noninvasive video-EEG, and assess its localizing value according to the surgical outcomes. DESIGN, TIME AND SETTING: A retrospective case analysis was performed at the Magnetoencephalography Laboratory, Guangdong 999 Brain Hospital, China between November 2001 and December 2005.PARTICIPANTS: A total of 618 epileptic patients undergoing magnetoencephalography. Spontaneous magnetic field signal was collected to analyze single dipole location. Of them, 149 patients with MRI-documented epilepsy underwent surgery. The history of disease was at least 2 years. The course of disease ranged 2-35 years, with an average of 9.5 years. METHODS: MEG was recorded by a 148-channels whole head type MEG system (Magnes WH2500, 4-D Neuroimaging, San Diego, CA, USA) in Magnetically Shielded Room (MSR, Germany). Sampling rate: 508.63 Hz, 30-minutes interictal MEG (Bandpass: 1.0-100 Hz). For magnetic source imaging, the nasion and preauricular points were applied as fiducials. Single equivalent current dipole (ECDs) and head sphere model were applied for analysis. Estimated ECDs were overlaid on T1-weighted MRI of each subject.MAIN OUTCOME MEASURES: Preoperative MEG, MRI, and video-EEG and postoperative follow-up were measured. RESULTS: The sensitivity of the interictal MEG for detecting epileptiform activity was found in 91% of the patient. In most cases, the equivalent dipoles were mainly distributed over the border and neighborhood of the structural lesions. By MEG, we were able to localize the resected region in a greater proportion of patients (62.4%, 93/149) than with noninvasive vedio-EEG (38.9%, 58/149) in all patients with MRI-documented lesions. A total of 89 patients were followed up from 3-35 months, averagely 9 months. Of the 89 patients, 72 patients (80.9%) had no postoperative seizures (EngelⅠ); 7 (7.9%) cases obtain Engel Ⅱ and Engel Ⅲ outcomes. Favorable outcomes were not seen in 10 patients (11.2%) cases (Engel Ⅳ and Engel Ⅴ). Total effective rate was 88.8% (EngelⅠ-Ⅲ). CONCLUSION: MEG is not only most useful for presurgical planning in epilepsy patients with MRI-documented lesions, but is also a noninvasive method to identify the spatial relationship between the lesion and epileptogenic zone, a precise localization of the epileptogenic zone is correlated to a favorable outcome.

To reduce the surgical complications and raise the successful rate of small intestinaltransplantation(SIT),32 rats received segmental isogenic SIT heterotopieally using modifiedMonehik and Russels'method with the successful rate being 96.9%.The survival rate of the ratsover 10 days was 81.25%with the longest survival over 500 days.It was indicated that those arethe keys of success of SIT such as adequate liquid supplement,excellent anastomosis of blood ves-sels and effective protection to the isograft,as well as the sterile principle and washing isograftregularly.