AIM: To establish the method for quality control of Quzhi Capsules(Radix et Rhizoma Rhei, Semen Cassiae, Herba Artemisiae Scopariae, Rhizoma Atractylodis, etc.). METHODS: Herba Artemisiae and Rhizoma Atractylodis in this medicine were identified by TLC. The content of chrysophanol was determined by HPLC on ODS column. The mobile phase was composed of methanol 0.5% phosphoric acid(85∶15,v∶v) and detection wavelength was at 254nm. RESULTS: The spots on TLC plates were clear without interference in the blank reference. The linearity of HPLC was good( r =0.9999), and the average recovery was 101.00%, RSD =1.3%. CONCLUSION: This method is simple, sensitive and accurate, and available for control of Quzhi Capsules.

Objective To investigate the correlation between gut microbiota and hepatitis B virus(HBV) infection at different stage. Methods 84 patients aged 25 ~ 40 were enrolled in the study,the ALT,AST and HBV antigens and antibodies qualitatively detected. Then they were divided into four groups based on HBV related antigens and antibodies qualitative detection according to guideline for chronic HBV infection:48 in the protective antibody positive group where vaccine injection was performed for producing protective antibodies ,14 in the HBV recovery group 9 in chronic HBV infection group And 13 in antibody negative group where vaccine injection was done. Their fecal samples were collected and total DNA were extracted and subjected to enterobacterial repetitive intergenic consensus sequence polymerase chain reaction(ERIC-PCR). After ERIC-PCR,gel electrophoresis and genetool software were used to analyse the differences in the amount of bands and the position of main bands between groups. Results The amounts of bands in the antibody negative and chronic HBV infected groups were significantly smaller than the protective antibody group and HBV recovery group. The position of main band in the protective antibody group and HBV recovery group were wildly spread within groups ,and most main bands in the HBV recovery group were above 282 bp. The main bands in the antibody negative and chronic HBV infected groups were centralized in 60 ~ 70 bp. Conclusions Compared with the protective antibody group and HBV recovery group ,the richness of gut microbiota in the antibody negative and chronic HBV infected groups are significantly decreased and lack of microbiota above 200 bp in ERIC-PCR.

Objective To compare the safety and effectiveness of temporary occlusion techniques with different balloons in performing cesarean section for patients with pernicious placenta previa.Methods The clinical data of 15 patients with pernicious placenta previa,who received cesarean section during the period from March 2014 to April 2016,were retrospectively analyzed.In order to reduce bleeding during operation and to preserve the uterus as far as possible,temporary occlusion of abdominal aorta with balloon was adopted in 7 patients (group A),and temporary balloon occlusion of bilateral internal iliac arteries was employed in 8 patients (group B).The intraoperative radiation dose,the amount of blood loss and blood transfusion during cesarean section,the hysterectomy rate,the postoperative hospitalization days,and the intervention-related complications were documented,and the results were compared between the two groups.Results All the 15 patients were suffered from central type of placenta praevia complicated by extensive placenta accreta.With the help of temporary balloon occlusion technique,the cesarean section was successfully accomplished in all 15 patients.Subtotal hysterectomy had to be done in 7 patients,including 4 patients of group A and 3 patients of group B,and every one patient from each group developed arterial thrombosis of lower limb.The intraoperative radiation dose of group A was significantly lower than that of group B (P＜0.01),besides the amount of blood loss and blood transfusion during cesarean section,the hysterectomy rate,the postoperative hospitalization days,and the intervention-related complications of group A were all lower than those of group B,but because of the limited number of cases these differences were not statistically significant (P＞0.05).Conclusion For the treatment of pernicious placenta previa,temporary balloon occlusion technique-assisted cesarean section is safe and effective.The intraoperative radiation dose in the group using temporary balloon occlusion of abdominal aorta is remarkably lower than that in the group using temporary balloon occlusion of bilateral internal iliac arteries.

Objective:To understand the situation of time limitation prescriptions of outpatients in our hospital. Methods:A ret-rospective study was conducted on 1 107 time limitation prescriptions from July 1st to 31th in 2015. Results: There were 1 107 time limitation prescriptions in 16 063 prescriptions (6. 89%), which included 902 patients, and among them, there were more than one time limitation prescription in one day for one patient. As the increase of age, the number of patients with time limitation prescriptions was increased. The number of time limitation prescriptions with the sum between 101 and 200 yuan was the most (34. 51%). The di-agnosis of top 10 of the drugs according to the doctor’ s advice and the actual top ten was substantially consistent, and among them, half were Chinese patent medicines. Conclusion:Time limitation prescriptions are the products of medical insurance policy. The man-agement of time limitation prescriptions can’ t be only defined as the quantity superposition of drugs, and it is necessary to generate in-dividualized strategy according to the properties of diseases, drugs and patients in order to obtain more reasonable management strate-gies, and the ultimate purposes are benefiting patients and obtaining more rational distribution of medical resource.

Objective To establish the methods for TLC identification and contents determination of Compound Yantongxiao Spray.Methods Flos Lonicera,Borneolum Syntheticum and Menthol in the spray were identified by TLC,and the content of chlorogenic acid was determined by HPLC.Results The TLC characteristic spots of Flos Lonicera,Borneolum Syntheticum and Menthol can be identified.For content determination,the linear range of chlorogenic acid was in 0.08～0.28 ?g(r=0.999 0),the average recovery of chlorogenic acid was 102.34 %(n=6)and RSD was 2.03 %.Conclusions The methods are simple,accurate,specific and reproducible,can be used for the quality control of Compound Yantongxiao Spray.

Objective To investigate the value of ureteropyeloscopy and B-type ultrasonography in the treatment of staghorn calculi. Methods Pneumatic lithotripsy was performed and the residual stone and stone fragments were removed under the assistance of ureteropyeloscope and B-type ultrasonograph in 40 patients. Results The stone fragments were small enough to remove and the calculi removal rate was 92.5% (37/40). The rate of complications mainly including hemorrhage and residual stones was 7.5% (3/40). Conclusion The application of ureteropyeloscopy and B-type ultrasonography in the treatment of staghorn calculi is safe and less invasive, and can reduce effectively the incidence of residual stones.

Objective To discuss the relativity between serum procalcitonin(PCT) level and renal impairment (RI) in critically ill patients. Methods A perspective study was performed in 60 critically ill patients. They were divided into 2 groups on the serum PCT level within 24 h: abnormal group (PCT ≥ 0.5 μg/L,38 cases) and normal group(PCT < 0.5 μg/L,22 cases). The serum creatinine (SCr) and blood urea nitrogen (BUN) were observed. Acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ ) of the patients were recorded. Calculated the sensitivity and specificity for prediction of RI as well as acute renal dysfunction (ARD). Results SCr [(188.91 ± 199.48) μmol/L]and BUN [(13.03 ±9.19)mmol/L]in abnormal group were significantly higher than those in normal group [(67.40 ± 15.35) μ mol/L and (6.91 ± 2.06) mmol/L](P< 0.01). The sensitivity of PCT forecast RI was 71.79%, and specificity was 52.38%. The sensitivity of PCT forecast ARD was 91.67% , and negative predictive value was 95.45%. Conclusion Serum PCT level has an important value for judgement of RI and ARD in critically ill patients.

Bladder pain syndrome or interstitial cystitis (BPS/IC) is a clinical diagnosis based primarily on refractory suprapubic pain,which is lack of effective treatment and a broad consensus of diagnostic criteria,because its etiology and pathophysiology remains unclear.In this review,it was discussed that recent research advancement regarding to definition,etiology,diagnosis and treatment of BPS/IC,through referring to the latest guidelines and literature on this disorder.