Hemoperfusion ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy

The US federal government regards health information technology (HIT) as an important action to stimulate growth of the health information economy.To implement HIT,the federal government proposed a tiered plan from organization guarantee,institutional innovation,HIT system design to economy stimulating mechanism,and to health care providers as well.In view of China's HIT development,it is proposed that HIT must proceed with healthcare reform in parallel.Stage-setup by the government,competition among enterprises,and active role of medical service providers constitute fundamental guarantee to ensure HIT go on wheels.Electronic health records of individuals should play key roles in promoting pragmatic and shared medicine health care information system.

BACKGROUND: The filling compactness can not be guaranteed due to apparatus retention in traditional lateral condensation.The warm gutter filling can achieve good results because of the easy changeable of gutta-percha in softening states.OBJECTIVE: To compare the warm gutta-percha fillings (Obtura Ⅱ and BeeFill 2 in1 filling) and cold lateral compacted gutta-percha with the bypassing root canal preparation in the intracanal separated instrument of oval canal.METHODS: Firstlythe 60 oval root canals of fiat mandibular central incisors were randomly divided into three groups, with 20 teeth in each group. After that, the root canal was filled with BeeFill 2 in1, Obtura Ⅱ condensation, and lateral condensation.The percentage of void at 5-mm to root tip versus cress section area was observed under a scanning electron microscope.RESULTS AND CONCLUSION: Under in vitro condition, the difference of percentage of void area versus root canal area between the Obtura Ⅱ condensation group and the lateral condensation group had significance (P=0.013), and the similar difference could be found between the BeeFill 2 in1 condensation group and the lateral condensation group (P=0.014), but the difference had no significance between the BeeFill 2 in1 condensation group and the Obtura Ⅱ condensation group (P=0.088).The results revealed that the root canal can be sealed accurately when establish hypass root next to the separated instrument and filled with BeeFill 2 in1 warm gutter filling.

Objective　To establish a simple assay for quick scanning of effective agents in growth inhibition and apoptosis induction. Methods　After observing the correlation between cell number and acid phosphatase activity, and between cell apoptosis and acid phosphatase activity, we established a microplate densimetry method to measure the acid phosphatase activity. We also compared the sensitivity with MTT assay and confirmed cell apoptosis by flow cytometry. Results　In two cell lines of cancer (Hep G2 and CBRH-7919 cells), the acid phosphatase activity was correlated well with the cell number, and was directly proportional to the cell number in the range of 0.5×103～0.7×103. The coefficient reached to 0.994. After stimulation of phorbel ester (TPA) for one hour, the acid phosphatase activity rose significantly. In contrast, after the inhibition of cell growth by As2O3, the acid phosphatase activity decreased significantly. The higher the concentration of As2O3, the lower the acid phophatase activity. The acid phophatase activity went down significantly when apoptosis in Hep G2 cells was only 3.98% and no apoptosis in CBRH-7919 cells after 24-hour treatment with As2O3. Conclusions　The acid phophatase activity of cell may be used to measure cell proliferation and apoptosis. Because of the easiness and quickness, acid phophatase assay might be an ideal approach to scan the effective growth inhibitors and apoptosis inducers in a large number of drugs.

ObjectiveTo identify the proteins presented variation during ischemia/reperfusion injury (I/RI) in liver transplantation (LT).MethodsAt Mar.2009,a total of nine liver sample tissues,three samples obtained from each donor liver at three different time points,were studied.The time points were as follows:(1) the time point immediately after donor liver was harvested (T1) ; (2) the time point just before the anastomosis of hepatic artery and potal vein (T2),during this time period the donor liver was kept in ice cold and transported,then back-table prepared,during time from T1 to T2,ischemia injury occurred; (3) the time after donor reconnected to the recipient (T3),during which the donor liver was subjected to reperfusion injury.The three time points represent normal control,ischemia injury,and reperfusion injury respectively.By using method of 2DE-MALDI-TOF and mass-spectrum,the differential expression of these protein at T1,T2,and T3 time point were compared.ResultsFrom the 1580 proteins that were isolated,19 ischemia/reperfusion injury (I/RI)-related proteins that varied markedly from time point T1 to T2 and from time point T2 to T3 were identified.They were metabolic enzyme,molecular chaperone,redoxase,cytoskeleton protein,signal transduction protein,and binding protein,respectively.The character of each protein was further analized based on their functions.ConclusionsFor the first time,we detected the protein related to I/RI during LT.We obtained independent proteomics information of ischemia injury,and reperfusion injury,respectively.Further functional analyses of these proteins may improve the understanding of the critical biological processes during I/RI.Medicine intervention on the I/RI-related proteins may result in improved survival of the recipients.

Objective To explore the effect of aspirin resistance on cardiovascular events in non-insulin-dependent diabetes melli-tus patients .Methods 496 patients with non-insulin-dependent diabetes mellitus received daily aspirin therapy (100mg/d) 4 weeks were recruited from Jun .2006 to Sept .2010 .Platelet aggregation was measured by light transmission aggregometry .The patients were divided into three groups :aspirin resistance group ,aspirin semi-resistance group and aspirin-sensitive group .The clinical bio-chemistry indications and the adverse cardiovascular events were collected .Results 11 .69% of patients were found to be resistant to aspirin therapy ,and 37 .90% of patients to be aspirin semi-responders .There were 67 .48% female in aspirin resistance group and aspirin semi-responders .The preprandial glucose of group AR and ASR was higher than that of group AS (P<0 .05) .The HbA1C and TG of group AR were higher than those of group AS(P<0 .05) .There was statistically significant difference of the rate of car-diovascular events .Conclusion The measure of platelet aggregation should be taken in the diabetic mellitus patients and pay atten-tion on the dose of aspirin recommended .Particular attention is should also devote to the incidence of adverse cardiovascular events .

Objective:To explore the reading feature of developmental dyslexia. Methods:820 children of grade 3~5 in 5 primary schools in Wuhan City were selected by cluster sampling; fifty-five children with developmental dyslexia were screened out according to ICD-10. One hundred and ten normal children with the same age, sex and socioeconomic status were chose as control. Results:The prevalence rate of developmental dyslexia is 6.7%. The score of 8 items of DCCC (Dyslexia Checklist for Chinese Children) of developmental dyslexics were mostly significantly higher than that of normal children (15.2?3.7/10.1?3.3, 21.6?3.7/13.4?4.9, 16.1?3.5/10.6?3.7, 16.8?3.6/11.7?3.1, 15.9?3.9/11.5?3.5, 21.4?4.3/14.5?4.6, 14.9?3.7/9.4?2.8, 23.2?4.4/15.9?4.7, all withP

In the Langendorff's perfused isolated rat hearts, the myocardial postischemic reperfusion injury was induced by reperfusion for 30 min after stoppage of perfusion for 40 min. The effects of naloxone on the lactic acid dehydrogenase (LDH) content, the myocardial Ca contents, the myocardial malondialdiehyde (MDA) production and the myocardial superox-ide dismutase (SOD) activity were observedduring reperfusion. The results show that naloxone significantly decreased cardiac LDH release, attenuated myocardial Ca accumulation, reduced MDA production and prevented redution of SOD activity in comparison with the control in perfused isolated rat hearts with global ischemia (40 min) and reperfusion (30 min). It suggests that naloxone has protective effects on myocardial reperfusion injury.

Food allergy( FA) is a growing health problem which affects young children′s growth. Re-cent studies have showed that leukotrienes can contract gastrointestinal smooth muscle,increase intestinal perm-bility,and it is obviously related to the development of FA gastrointestinal disorder. Leukotriene receptor antago-nists have provided a good therapeutic option and showed clinical benefits in the management of the FA gastroin-testinal disorder.

Epstein-Barr Virus (EBV) is the first human virus related to oncogenesis.EBV infection is associated with inflammatory bowel disease (IBD) with unknown causality.The prevalence of EBV in intestinal tissue from patients with IBD is significantly higher and related to the exacerbation of the disease and refractory IBD.Immunosuppressive therapy has improved outcomes associated with IBD.However,it is also associated with an increased risk of opportunistic infection,and lymphoproliferative disorders (LDs) maybe due to EBV infection.Here we review our current understanding of the pathogenesis of EBV infection in colonic mucosal inflammation,EBV-induced disease exacerbation,lymphomagenesis in IBD,and clinical approaches therefrom.