Hemoperfusion ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy

Food allergy( FA) is a growing health problem which affects young children′s growth. Re-cent studies have showed that leukotrienes can contract gastrointestinal smooth muscle,increase intestinal perm-bility,and it is obviously related to the development of FA gastrointestinal disorder. Leukotriene receptor antago-nists have provided a good therapeutic option and showed clinical benefits in the management of the FA gastroin-testinal disorder.

Neurally ajd usted ventilatory assist( NAVA) uses the electrical activity of the diaphragm as a neural trigger to synchronize mechanical ventilatory breaths with the patient′s neural respiratory drive. Some studies have shown that compared with pressure-support ventilation,NAVA can improve patient-venti-lator synchrony while maintaining spontaneous breathing,unload respiratory musculaturei n term adn prte erm i nfants.Further studies are needed to determ ine whether NA VA will have significant impact on the overall outcomes of neonates.

BACKGROUND: The filling compactness can not be guaranteed due to apparatus retention in traditional lateral condensation.The warm gutter filling can achieve good results because of the easy changeable of gutta-percha in softening states.OBJECTIVE: To compare the warm gutta-percha fillings (Obtura Ⅱ and BeeFill 2 in1 filling) and cold lateral compacted gutta-percha with the bypassing root canal preparation in the intracanal separated instrument of oval canal.METHODS: Firstlythe 60 oval root canals of fiat mandibular central incisors were randomly divided into three groups, with 20 teeth in each group. After that, the root canal was filled with BeeFill 2 in1, Obtura Ⅱ condensation, and lateral condensation.The percentage of void at 5-mm to root tip versus cress section area was observed under a scanning electron microscope.RESULTS AND CONCLUSION: Under in vitro condition, the difference of percentage of void area versus root canal area between the Obtura Ⅱ condensation group and the lateral condensation group had significance (P=0.013), and the similar difference could be found between the BeeFill 2 in1 condensation group and the lateral condensation group (P=0.014), but the difference had no significance between the BeeFill 2 in1 condensation group and the Obtura Ⅱ condensation group (P=0.088).The results revealed that the root canal can be sealed accurately when establish hypass root next to the separated instrument and filled with BeeFill 2 in1 warm gutter filling.

ObjectiveTo identify the proteins presented variation during ischemia/reperfusion injury (I/RI) in liver transplantation (LT).MethodsAt Mar.2009,a total of nine liver sample tissues,three samples obtained from each donor liver at three different time points,were studied.The time points were as follows:(1) the time point immediately after donor liver was harvested (T1) ; (2) the time point just before the anastomosis of hepatic artery and potal vein (T2),during this time period the donor liver was kept in ice cold and transported,then back-table prepared,during time from T1 to T2,ischemia injury occurred; (3) the time after donor reconnected to the recipient (T3),during which the donor liver was subjected to reperfusion injury.The three time points represent normal control,ischemia injury,and reperfusion injury respectively.By using method of 2DE-MALDI-TOF and mass-spectrum,the differential expression of these protein at T1,T2,and T3 time point were compared.ResultsFrom the 1580 proteins that were isolated,19 ischemia/reperfusion injury (I/RI)-related proteins that varied markedly from time point T1 to T2 and from time point T2 to T3 were identified.They were metabolic enzyme,molecular chaperone,redoxase,cytoskeleton protein,signal transduction protein,and binding protein,respectively.The character of each protein was further analized based on their functions.ConclusionsFor the first time,we detected the protein related to I/RI during LT.We obtained independent proteomics information of ischemia injury,and reperfusion injury,respectively.Further functional analyses of these proteins may improve the understanding of the critical biological processes during I/RI.Medicine intervention on the I/RI-related proteins may result in improved survival of the recipients.

Objective　To establish a simple assay for quick scanning of effective agents in growth inhibition and apoptosis induction. Methods　After observing the correlation between cell number and acid phosphatase activity, and between cell apoptosis and acid phosphatase activity, we established a microplate densimetry method to measure the acid phosphatase activity. We also compared the sensitivity with MTT assay and confirmed cell apoptosis by flow cytometry. Results　In two cell lines of cancer (Hep G2 and CBRH-7919 cells), the acid phosphatase activity was correlated well with the cell number, and was directly proportional to the cell number in the range of 0.5×103～0.7×103. The coefficient reached to 0.994. After stimulation of phorbel ester (TPA) for one hour, the acid phosphatase activity rose significantly. In contrast, after the inhibition of cell growth by As2O3, the acid phosphatase activity decreased significantly. The higher the concentration of As2O3, the lower the acid phophatase activity. The acid phophatase activity went down significantly when apoptosis in Hep G2 cells was only 3.98% and no apoptosis in CBRH-7919 cells after 24-hour treatment with As2O3. Conclusions　The acid phophatase activity of cell may be used to measure cell proliferation and apoptosis. Because of the easiness and quickness, acid phophatase assay might be an ideal approach to scan the effective growth inhibitors and apoptosis inducers in a large number of drugs.

OBJECTIVE: To provide valuable reference for China in formulating and evolving pricing policy on patent drugs.METHODS: The current pricing policy on patent drugs in Sweden and its outcome were analyzed,and then its implication for Chinese medical & health market were summarized.RESULTS & CONCLUSIONS: We suggest that market information should be taken into full consideration and drugs price supervision system should be established in making pricing policy so as to avoid information asymmetry in pricing game and ensure effective utilization of social medical insurance resources.

OBJECTIVE: To evaluate the pricing policy on the patent drugs in UK.METHODS: The current pricing policy on patent drugs in UK and the outcome of the policy were analyzed and its characteristics and applicable condition were summarized.RESULTS & CONCLUSIONS: Current pricing policy on patent drugs in UK provided incentives for the research & development as well as innovation of drugs and it helped to keep UK's pharmaceutical industries in the global leading level.However,there is no mechanism within it to take explicit account of therapeutic value of drugs.Also,it provides weak incentives to invest in the most cost-effective yet clinically demanded drugs and it has limited effect on price control of drugs.

In the Langendorff's perfused isolated rat hearts, the myocardial postischemic reperfusion injury was induced by reperfusion for 30 min after stoppage of perfusion for 40 min. The effects of naloxone on the lactic acid dehydrogenase (LDH) content, the myocardial Ca contents, the myocardial malondialdiehyde (MDA) production and the myocardial superox-ide dismutase (SOD) activity were observedduring reperfusion. The results show that naloxone significantly decreased cardiac LDH release, attenuated myocardial Ca accumulation, reduced MDA production and prevented redution of SOD activity in comparison with the control in perfused isolated rat hearts with global ischemia (40 min) and reperfusion (30 min). It suggests that naloxone has protective effects on myocardial reperfusion injury.

Objective To evaluate CT features of hemorrhagic and necrotic polyps of maxillary sinus and nasal cavity.Methods Twenty-nine cases of hemorrhagic and necrotic polyps proved pathologically were collected in this study.Axial,coronal,plain and / or contrast enhanced thin-slice CT scans were performed in all the cases.Results The density of lesion was inhomogene (26/29).The lesions had clear boundary and slight enhancement on contrast-enhanced scan.Expanding of nasal and /or antronasal cavity,compressive bony absorption were often seen in inter-wall of maxillary sinus(13/29).The bony destruction in most of the cases were showed with bony sclerosis(25/29).Conclusion The diagnosis of hemorrhagic and necrotic polyps can be confirmed by CT examination.