OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use

Catheter-Related Infections ; drug therapy ; prevention & control ; Catheters, Indwelling ; adverse effects ; Humans ; Intensive Care Units, Neonatal ; Thrombosis ; prevention & control

Transjugular intrahepatic portosystemic shunt (TIPS) has been developed for two decades in order to treat portal hypertension accompanying with upper gastrointestinal hemorrhage and refractory ascites.TIPS may cause the development of hepatic encephalopathy (HE).TIPS induces portalsystemic shunt and leads to hemodynamics chaos. Hepatic encephalopathy belongs to the complications of the portal hypertension. There are many possible predictors of the post-TIPS HE. The relationship between TIPS and HE was reviewed.

Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.

Objective To establish the quality standards for Shufeng Zhike Extract;To improve the controll ability of the extract and ensure medicine efficacy. Methods To establish a HPLC method for simultaneous differentiation of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum in Shufeng Zhike Extract. Phenomenex Kintex C18 column (4.6 mm×100 mm, 2.6 μm) was used. Acetonitrile-0.05%phosphoric acid was as the mobile phase in gradient elution (0–25 min, 10%acetonitrile; 25–26 min, 10%→14% acetonitrile; 26–35 min, 14% acetonitrile; 35–36 min, 14%→34% acetonitrile;36–55 min, 34%acetonitrile) at flow rate of 0.8 mL/min, and column temperature was 40 ℃. The detection wavelength was 250 nm. To establish a HPLC method for simultaneous determination of Paeoniflorin and Prim-O-glucosylcimifugin in Shufeng Zhike Extract, Phenomenex Luna C18 column (4.6 mm×150 mm, 5 μm) was used; acetonitrile-0.05%phosphoric acid (12:88) was as the mobile phase at flow rate of 1 mL/min; column temperature was 40 ℃; the detection wavelength was 250 nm. Results The chromatographic peak separation with HPLC method for simultaneous differentiate of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizoma et Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurumin in Shufeng Zhike Extract was clear and negative control had no interference. The linear range of Paeoniflorin was 0.035 93–2.514 8 μg (r=0.999 5), and the average recovery was 100.54%(n=6). The linear range of Prim-O-glucosylcimifugin was 0.006 7–0.67 μg (r=0.999 5), and the average recovery was 100.39%(n=6). Conclusion The established quality standards are simple, reliable, and accurate. It can rapidly identificate Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum, and suitable for rapid determination of Paeoniflorin and Prim-O-glucosylcimifugin, which can provide the basis for the quality inspection of Shufeng Zhike Extract.

Objective To explore psychological defense mechanism and its relationship with personality in patients with hysteria(HyS). Method Both HyS patients (n=30) and normal control(NC, n=66) comple-ted Defense Style Questionnaire (DSQ) and Eysenck Personality Questionnaire(EPQ) . Results The scores of projection, isolation, somatization and the factor scores of immature defenses were significantly higher in HyS pa-tients(3.64±1.45, 5.63±2.29,5.33±2.36 and 4.74±1.27, respectively) than those in NC [(2.61±1.09,4.33±2.02, 3.86±1.60 and 3.98±0.91, respectively) (all P<0.01)]. The scores of undoing, idealization, pseudo-altruism and the factor scores of neurotic defenses were also significantly higher in HyS patients compared with NC(P<0.01). The scores of suppression, humor and the factor scores of mature defenses and the lie were found lower significantly in HyS patients compared with NC (P<0.05). The scores of the neuroticism, psychoticism and lie subscale of EPQ in HyS patients were significantly higher than that of NC(P<0.01). The scores of extro-vert subscale were not found difference significantly between HyS patients and NC. The results of correlation analy-sis showed that the scores of the psychoticism subscale of EPQ in HyS patients correlated negatively with the factor scores of mature mechanism and the scores of suppression. Conclusion The HyS patients were more likely than the normal control to endorse the immature mechanism and neurotic defense and less likely to endorse the mature mechanism, which related with unhealthy personality, especially the psychoticism.

In recent years ,matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a new organic soft ionization mass spectrometry which can identify and classify microorganism by directly using the sam-ple itself or cultivate colonies on petri dish in a few minutes .This new and simple method reduces the cost of consumables and the time of diagnosis greatly .Its reliability and accuracy have been shown in many studies and different system devices have al-ready been on the market .In the near future ,MALDI-TOF MS will have a broader prospect .This mass spectrometry will be-come an effective and fast microbial identification technology to replace the conventional method .In this article ,we will review the principles of MALDI-TOF MS ,its development and application ,its advantages and shortcomings which compared with tra-ditional methods at the present stage ,and the emphasis will focus on the application characteristics of MALDI-TOF MS in mi-crobial systems .

Objective To investigate the clinical characteristics of the patients with central brain herniation,and the operation effect of high coronary craniotomy for bilateral decompressive craniectomy.Methods A retrospective analysis was conducted on 26 cases of central brain herniation.The effectiveness of high coronary craniotomy for bilateral decompressive craniectomy and the influential factor of prognosis in patients with central brain herniation were analyzed.Results According to the Glasgow outcome score (GOS) 6 months after operation,5 cases were recovered well,6 cases moderately disabled,4 cases severely disabled,3 cases vegetative survived and 8 cases died.Conclusions High coronary craniotomy and timely and effective bilateral decompressive craniectomy are the most important treatment in patients with central brain herniation.The prognosis is unfavourable prognosis in patients with central brain herniation within 1 h after injury and older than 75 years old.