Objective To construct an efficient SALL4 shRNA vector and transfect it into THP-1 cells for investigating the effect of SALL4 in leukemia. Methods Four SALL4-specific siRNA to aim at different SALL4 mRNA target sites and a negative control siRNA were designed, and pGPU6/GFP/Neo/SALL4 shRNA vectors were constructed. THP-1 cells were transfected and the expression of SALL4 in shRNA detected, and blank control and negative control were also designed. Results The results of real time quantitive PCR and Western blotting both exhibited that the interference effect of pGPU6/GFP/Neo/SALL4 shRNA-B vector was optimal targeting to mRNA-1122 target site and down-regulated the expression of SALL4 more significant(P <0.05). Conclusion Successfully construction of SALL4 siRNA vector by choosing SALL4 shRNA-B would be useful to accomplish study of SALL4.

China ; Emigration and Immigration ; Immunization Programs

Emergency Treatment ; methods ; Humans ; Seizures ; therapy ; Status Epilepticus ; therapy

Objective To investigate the clinical correlation of intestinal bacterial translocation(BT) and in-testinal barrier of pediatric intestinal surgery,and to observe the relationship between BT and postoperative infection.Methods The plasma D-lactic acid,LPS level of 61 children with intestinal surgery in our hospital were detected before and after surgery.Polymerase chain reaction (PCR) was used to test blood bacterial DNA,in order to judge whether there is BT.Results The PCR results of preoperative children were negative,the PCR positive for 3 cases.According to the PCR results,postoperative systemic inflammatory response syndrome(SIRS) and complications of infection of PCR positive group were obviously higher than those of PCR negative group(P ＜ 0.01).And plasma D-lactic acid,LPS inspection of PCR positive group were obviously higher than PCR negative group(P ＜ 0.01).Conclusion Intestinal barrier damage has close relationship with BT in children after intestinal surgery,SIRS and infection complications also has a direct relationship with BT.

Objective To observe the treatment of continuous passive motion(CPM) on talipes valgus in the chil-dren with cerebral palsy. Methods There were 57 children in this research including normal group and valgus group, the children in valgus group were randomly divided into control group, non-CPM group and CPM group. The control group didn’t receive any rehabilitation treatment. The non-CPM group received conventional rehabilita-tion treatment. The CPM group received conventional rehabilitation and CPM therapy. Evaluate the children in nor-mal group with plantar pressure analysis and evaluate each patient before treatment two and four months after treat-ment with plantar pressure analysis. Results Compared with the normal group, the percentage of medial midfoot (MMF) pressure, HEEL pressure, initial contact phase(ICP) and forefoot contact phase(FFCP) were obviously higher in valgus groups. On the other hand, the percentage of lateral forefoot ( LFF) pressure, lateral midfoot (LMF) pressure and foot flat phase(FFP) were obviously lower in valgus groups(P < 0. 05). The percentage of MMF pressure, and FFP became lower after treatment in both CPM group and non-CPM group while the percentage of ICP and FFCP became higher(P < 0. 05). In CPM group there was a more obvious change with the percentage of MMF pressure, ICP and FFCP compared with non-CPM group and the control group(P < 0. 05). Conclusion U-sing CPM can alleviate muscle spasms and contracture, correct the deformity of midfoot, enhance the muscle group coordination on foot and further more improve gait of patients with cerebral palsy.

OBJECTIVE： To discuss the selectivity of enatiomers of clinically used asymmetric drugs.METHODS： To exsamplify this issue in respect to pharmacokinetic characteristics,pharmacodynamic properties and clinical application of asymmetric drugs.RESULTS ＆ CONCLUSION： The enatiomers of asymmetric drugs assume complex pharmacokinetic and pharmacodynamic characteristics.Thorough study of enatiomers of asymmetric drugs will exert great influence on rational use of asymmetric drugs.

Objective To investigate the effects of angiotensinⅡ (AngⅡ) on the Apelin mRNA and APJ mRNA expression in pulmonary microvascular endothelial ceils (PMVECs) in rats. Methods Pulmonary microvascular endothelial ceils obtained from 24 h old neonatal SD rats were cultured in DMEM liquid culture medium. The 2nd-4th generation PMVECs were inoculated on 6-well plates (5×105). The experiment was performed in two parts. In part Ⅰ different concentration of AngⅡ 0, 10-9, 10-8, 10-7, 10-6mol/L (group Ⅰ- Ⅴ) were added into the PMVECs. The expression of Apelin mRNA and APJ mRNA was determined at 24 h after addition of Ang Ⅱ by RT-PCR. In part Ⅱ the cells were exposed to 10-7 mol/L Ang Ⅱ. The expression of Apelin mRNA and APJ mRNA was determined immediately and at 1, 6, 12, 24, 48 h(group Ⅰ-Ⅵ) after addition of Ang Ⅱ by RT-PCR. Results In part Ⅰ Apelin mRNA expression was significantly higher in group Ⅱ (Ang Ⅱ 10-9 mol/L) but lower in group Ⅲ-Ⅴ (AngⅡ 10-8, 10-7, 10-6 mol/L) than in group Ⅰ (control, Ang Ⅱ 0 mol/L). The APJ mRNA expression was significantly lowered in group Ⅱ-Ⅴ in a dose-dependent manner as compared with control group (group Ⅰ). In partⅡ beth Apelin mRNA and APJ mRNA expression exhibited a bi-phasic response to AngⅡ 10-7 mol/L, increased at first and was then decreasing with time. The Apelin mRNA and APJ mRNA expression reached the peak at 1 h of incubation with Ang Ⅱ respectively. Conclusion Ang Ⅱ decreases both Apelin mRNA and APJ mRNA expression in PMVECs in a dose and time dependent manner. The down-regulation of Apelin mRNA and APJ mRNA expression may be involved in the mechanism of injury to PMVECs.

In the present study,the trachea of 17 new borns and 10 animals, including rabbits, dogs and monkeys were used. The neural elements were visualized by Cajal-Faworsky silver impregnation and osmic acid techniques. Specimens were cut in to sections by freezing microtome and stained by Gros—Bielchowsky method.Branches from the recurrent laryngeal and vagus nerves pass to the trachea,dividing and rejoining to form a mesh-like plexus outer to the cartilage. Fibers of the posterior parts of the plexus appear as a well-defined longitudinal chain of nerves. The branches from this plexus re-form a primary, secondary and tertiary plexuses in the membrane of cartilage plates, in the muscle, the submucous regions and the mucosa.Parasympathetic effector neurons were mostly found in the posterior and lateral wall, on rare occasions the anterior wall.A profuse supply of predominantly unmyelinated fibres innervates the muscle,blood vessels and glands, and the plexuses supplying these structure apparently communicate with each other. They also contain some thinly myelinated fibres.In the layer of smooth muscle the nerve fibres give off fine collaterals and end in knob-like or arrowheaded swellings. The fibres of subepithelial plexuses branch rectangularly and pass between the epithelial cells. All these fibers terminate in knobs which lie among the deep epithelial cells.