Objective To construct an efficient SALL4 shRNA vector and transfect it into THP-1 cells for investigating the effect of SALL4 in leukemia. Methods Four SALL4-specific siRNA to aim at different SALL4 mRNA target sites and a negative control siRNA were designed, and pGPU6/GFP/Neo/SALL4 shRNA vectors were constructed. THP-1 cells were transfected and the expression of SALL4 in shRNA detected, and blank control and negative control were also designed. Results The results of real time quantitive PCR and Western blotting both exhibited that the interference effect of pGPU6/GFP/Neo/SALL4 shRNA-B vector was optimal targeting to mRNA-1122 target site and down-regulated the expression of SALL4 more significant(P <0.05). Conclusion Successfully construction of SALL4 siRNA vector by choosing SALL4 shRNA-B would be useful to accomplish study of SALL4.

China ; Emigration and Immigration ; Immunization Programs

Objective To investigate the levels of inflammatory cytokines in children with low absolute lymphocyte count (ALC).Methods 56 patients with low ALC as the patients group and 20 children with healthy physical examination as the control group were enrolled.Several inflammatory cytokines,namely IL-1,IL-6,IL-8 and TNF-βwere measured with enzyme linked immu-nosorbent assay(ELISA)kits.High sensitive C reactive protein(Hs-CRP)was measured by the immunoturbidimetry(ITM)kit with the automatic biochemical analyzer.Results The concentrations of IL-1,IL-6,IL-8 and TNF-βin the patients were(0.096±0.012) ng/mL,(0.118±0.026)ng/mL,(0.388 ±0.069)ng/mL and(1.256 ±0.245 )ng/mL respectively,which had no statistical differ-ences as compared with the control group(P >0.05);the concentration of HsCRP in the patients group was(5.7±2.8)mg/L,while which in the control group was(4.8±2.6)mg/L,showing no statistically significant difference between them(P >0.05).Conclusion The levels of the cytokines in low ALC patients are not increased,which may be related with the other factors.Actively using tra-ditional Chinese medicine treatment is beneficial to the recovery of disease.

Previous studies have showed that sulfonylureas possess a bidirectional effect on cardiovascular system:they can raise the coronary artery tone,aggravate ischemic injury to the cardiovascular tissues,and accelerate cardiac hypertrophy through closing cardiovascular ATP-sensitive K+ channels;meanwhile,they can also regulate glucolipid metabolism,modulate inflammatory reaction and inhibit atherosclerosis through activating peroxisome proliferator-activated receptors.Clinical-epidemiological study showed different opinions on whether sulfonylureas can increase the mortality of patients with cardiovascular diseases.

This paper investigates 240 cases of medical disputes and interviews involved patients with questionnaires,explores patients′ behaviors,attitudes,and complaints in medical disputes,and analyzes the reasons and motivations for those behaviors and attitudes,in order to provide reference for reduce medical disputes and improve physician-patient relationship.

Objection: To study the characteristics of shame of patients with neurosis. Method: 52 patients with nuclear neurosis were evaluated by SCL-90, Shame Scale, and Children Trauma Questionnaire. The control group (n=30) was evaluated just by Shame Scale. Results:The total score of Shame Scale and the subscores of each factor were all higher in neurotic patients than in control group (total score 69.0?20.4/48.7?10.2, personality 29.4?9.7/19.8?5.8, behavior 23.5?6.5/18.0?4.2, body 8.3?3.4/5.7?2.2, family 7.7?3.9/5.2?1.7, t=3.15~5.09,P=0.001or

Objective To evaluate the performance of T-SPOT.TB Test and serological tuberculosis antibody(TB-Ab) in tuberculosis-assisted diagnosis.Methods Patients were diagnosed as tuberculosis from 2015 January to 2016 March in the hospital.T-SPOT.TB and tuberculosis antibody detection were performed for those patients.Results In 112 cases of tuberculosis patients,101 cases of T-SPOT.TB test were positive and the sensitivity was 90.18%,32 cases of tuberculosis antibody detection were positive and the sensitivity was 28.57%.In 41 cases of pulmonary tuberculosis,T-SPOT.TB assay showed positive results in 40 patients and the sensitivity was 97.56%,tuberculosis antibody detection showed positive results in 18 patients and the sensitivity was 43.9%;in 71 cases of extrapulmonary tuberculosis,T-SPOT.TB assay showed positive results in 61 patients and the sensitivity was 85.91%,tuberculosis antibody detection showed positive results in 14 patients and the sensitivity was 19.7%.Conclusion T-SPOT.TB was superior to serological tuberculosis antibody(TB-Ab),and the diagnosis of active tuberculosis was higher,and the sensitivity of T-SPOT.TB was higher than that of T-SPOT.TB value.

10%～20% of acute clinical sporatic hepatitis is hepatitis E (HE). HE has a severe diaease low mortality in general population of about 0.5～1.5%. It has been reported as severe disease and the mortality increased to 20% in pregnant women and old people. China is high-epidemic for hepatitis E virus (HEV). HEV is mainly transmitted through faecaloral route,data also showed that HEV could be transferred through blood donation. The epidemiology,diagnostics and vaccine development of HE is reviewed in this paper.

Objective:To evaluate the effect of Ginseng-Sini in early goal directed treatment septic shock under PICCO.Methods: Forty four patents with septic shock were randomly divided into control group, which treated with Ginseng-Sini on the basis of routine treatment under PICCO, observation groups, which treated with only the basis of routine treatment under PICCO. Before and after the drugs were given for 6 hours, HR, IMAP, CVP, SpO2, Lac, CI, ITBVI, SVRI were monitored.Results: Before treatment, HR, IMAP, CVP, SpO2, Lac, CI, ITBVI, SVRI of the observation group were not different from the control group(P>0.05); after 6 hours of treatment, CI of the observation group were higher than that of the control group(P<0.05).Conclusion: Ginseng-Sini in the early goal directed of septic shock is effective, especially in improving tissue perfusion.

Objective To investigate the effects of angiotensinⅡ (AngⅡ) on the Apelin mRNA and APJ mRNA expression in pulmonary microvascular endothelial ceils (PMVECs) in rats. Methods Pulmonary microvascular endothelial ceils obtained from 24 h old neonatal SD rats were cultured in DMEM liquid culture medium. The 2nd-4th generation PMVECs were inoculated on 6-well plates (5×105). The experiment was performed in two parts. In part Ⅰ different concentration of AngⅡ 0, 10-9, 10-8, 10-7, 10-6mol/L (group Ⅰ- Ⅴ) were added into the PMVECs. The expression of Apelin mRNA and APJ mRNA was determined at 24 h after addition of Ang Ⅱ by RT-PCR. In part Ⅱ the cells were exposed to 10-7 mol/L Ang Ⅱ. The expression of Apelin mRNA and APJ mRNA was determined immediately and at 1, 6, 12, 24, 48 h(group Ⅰ-Ⅵ) after addition of Ang Ⅱ by RT-PCR. Results In part Ⅰ Apelin mRNA expression was significantly higher in group Ⅱ (Ang Ⅱ 10-9 mol/L) but lower in group Ⅲ-Ⅴ (AngⅡ 10-8, 10-7, 10-6 mol/L) than in group Ⅰ (control, Ang Ⅱ 0 mol/L). The APJ mRNA expression was significantly lowered in group Ⅱ-Ⅴ in a dose-dependent manner as compared with control group (group Ⅰ). In partⅡ beth Apelin mRNA and APJ mRNA expression exhibited a bi-phasic response to AngⅡ 10-7 mol/L, increased at first and was then decreasing with time. The Apelin mRNA and APJ mRNA expression reached the peak at 1 h of incubation with Ang Ⅱ respectively. Conclusion Ang Ⅱ decreases both Apelin mRNA and APJ mRNA expression in PMVECs in a dose and time dependent manner. The down-regulation of Apelin mRNA and APJ mRNA expression may be involved in the mechanism of injury to PMVECs.