Objective To investigate the cumulative organ damage of systemic lupus erythematosus (SLE) and It's affecting factors.Methods 162 cases of SLE patients were reviewed and evaluated in the cumulative organ damage of them . At the same time, It's affecting factors were analysed by multifactorial analysis.Results The cumulative organ damage of SLE was obviously correlated with the first time of treatment, treatment with CTX ,the numbers of recurrence rate and level of complements;but were not correlated with disease duration and cumulative dosage of corticosteroid.Conclusions The cumulative organ damage was one of the evaluating factors of SLE disease, and it's occurence and development were affected by multiple factors .So, the patients should be treated with hormone and control the beneficial factors to protect organs,such as,observation on complemets level,high dosage cyclophosphamide pulse treatment etc.

Objective To assess the value of contrast-enhanced ultrasonography (CEUS) before percutaneous radiofrequency ablation (RFA) in treating patients with liver metastases. Methods A total of 267 patients with 485 liver metastatic lesions were treated with percutaneous RFA in authors ’ department during the period from July 2001 to December 2012. Among them , 180 patients with 251 lesions received CEUS examination before RFA and based on CEUS findings the treatment scheme was made (CEUS group), and other 87 patients with 234 lesions without use of preoperative CEUS were used as control group. No significant differences in clinical data existed between the two groups (P<0.05). Contrast-enhanced CT/MRI, CEUS and laboratory tests were regularly employed to evaluate the clinical results after RFA therapy. Results In CEUS group, 25.1% of the lesions (63/251) determined by CEUS were 3 mm larger than that determined by conventional US. 8.8%of the lesions (22/251) were more clear on CEUS findings. In addition, 41 new lesions were detected only on CEUS. No significant differences in early tumor necrosis rate existed between the two groups: 95.2%(239/251) vs. 92.7%(217/234)(P>0.05). The local recurrence rate of CEUS group was lower than that of the control group: 12.4% (46/234) vs. 19.7%(31/251) (P < 0.05). No significant differences in the recurrence time existed between the two groups (P > 0.05). Conclusion CEUS performed before RFA treatment for patients with liver metastases is very useful for accurately judging the size and number of the lesions, which is very helpful in making therapeutic scheme. Therefore, preoperative CEUS can significantly increase early tumor necrosis rate and decrease the local recurrence rate.

BACKGROUND:Adipose-derived mesenchymal stem cells have the ability to self-renew and have pluripotent potential under specific conditions in vitro, which have broad application prospects in clinical practice. However, isolation and culture of adipose-derived mesenchymal stem cells stil appear to have many difficulties and shortcomings. OBJECTIVE:To isolate and culture rabbit adipose-derived mesenchymal stem cells in vitro in order to study their morphology, cellsurface markers and biological properties as wel as to investigate the osteogenic and adipogenic potentials of adipose-derived mesenchymal stem cells in vitro. METHODS:Primary adipose-derived mesenchymal stem cells were isolated from the subcutaneous adipose tissue of posterior cervical region from New Zealand white rabbits and digested by 0.1%col agenase I. The cells were passaged and amplified by the trypsin digestion. The passage 4 adipose-derived mesenchymal stem cells were induced to differentiate after exposure to adipogenic or osteogenic medium. The oil red O staining, alkaline phosphatase and alizarin red staining were used to detect the results. The cellviability was detected by the cellcounting kit 8 method to drawn the growth curve. cellsurface markers were examined using flow cytometry. RESULTS AND CONCLUSION:The adipose-derived mesenchymal stem cells isolated from the subcutaneous adipose tissue of rabbits exhibited a fusiform adherent growth in a vortex pattern, and had a strong capability of proliferation that could be passaged stably to the 10th generation. Flow cytometry results showed that the cells highly expressed CD29, CD90, CD44, but lowly expressed CD45 and CD34. After adipogenic induction, the adipose-derived mesenchymal stem cells were positive for oil red O staining;after osteogenic induction, the cells were both positive for alkaline phosphatase and alizarin red staining. These findings suggest that the adipose-derived mesenchymal stem cells were successful y isolated and cultured from the subcutaneous adipose tissue of rabbits, and these cells are pluripotent with the potential to differentiate into adipocytes and osteoblasts, which are expected to be ideal seed cells for bone tissue engineering.

Objective:To probe the effect of Alisol Monoacetate A and Alisol Monoacetate B on the synthesis and metabolism of cholesterol in HepG2 cell line.Methods:Controlled with Lipitor,different concentration of Alisol Monoacetate A and B were added to HepG2 cell line model,then collected and detected the contents of cholesterol in the cell lysate and cultured medium after 24h's cultivation.Results:The cytotoxicity of Alisol Monoacetate A and B appeared at least 10% when its concentration was higher than 10?M,more than 70% when its concentration was 50?M.The contents of cholesterol in HepG2 cell lysate increased from 24.4,26.7,32.3 and 38.3?g/mg protein corresponding with the concentration of 0?M,3?M,10?M and 20?M respectively,which showed the positive dose-effect relationship.However,the contents of cholesterol in the cultured medium manifested no difference.Conclusion:Alisol Monoacetate A and B could enhance the metabolic activity of mitochondria and increase the synthesis of cholesterol in HepG2 cell line.

Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species (ROS) and glutathione (GSH) production and genomic DNA oxidation in HT-1080 cells.Methods: The ROS generation induced by H2O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2O2.Results: Then-hexane, 85％ aqueous methanol andn-butanol fractions (0.05 mg/mL concentrations) inhibited H2O2-induced ROS generation by 63％, 35％ and 45％, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts (P<0.05). Supplementation of cells withn-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL (P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage (P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by then-hexane fraction and this fraction may contain valuable active compounds.

No abstract available.
Nevus, Pigmented*

No abstract available.

BACKGROUND: There have been evidences that inactivation of tumor supressor genes such as p16 and pRb develops human malignancies. p16 inhibits the cyclin D/cyclin-dependant kinase 4 complex which phosphorylates pRb, thus negatively regulating cell cycle progression. Rb gene inactivation has been observed in various tumor and reported to be reciprocally correlated with p16 expression. The purpose of this study is to evaluate aberrant expression of p16 and pRb in acute leukemia by immunohistochemial stain in clot or biopsy section of the bone marrow. METHODS: For detection of p16 and pRb, immunohistochemical staining with anti-human mouse monoclonal antibodies to p16 and pRb were done on 62 bone marrow paraffin sections from leukemic patients. In acute lymphoblastic leukemia (ALL), immunophenotyping was performed with indirect immunofluorescent assay and clinical parameters were analyzed depending on status of p16 expression. RESULTS: In 28 cases (45%), abnormal expression of p16 or pRb were observed. Twenty three of those cases (37%) showed aberrancy only in one protein, in which case, staining intensity was generally more stronger than that of normal expression in both proteins. The proportion of abnormal p16 expression was 30% and 15%, and that of pRb was 30% and 36% in ALL and in acute myelocytic leukemia, respectively. There was no significant clinical correlation between aberrancy of p16 and clinical parameters of acute leukemia. CONCLUSION: Our results suggest that loss of the p16 and pRb function may contribute to pathogenesis of acute leukemia. Further study is needed in more cases of acute leukemia to evaluate clinical significance of p16 or pRb aberrancy, particularly in ALL.
Animals ; Antibodies, Monoclonal ; Biopsy ; Bone Marrow ; Cell Cycle ; Cyclins ; Genes, Retinoblastoma ; Humans ; Immunophenotyping ; Leukemia* ; Leukemia, Myeloid, Acute ; Mice ; Paraffin ; Phosphotransferases ; Precursor Cell Lymphoblastic Leukemia-Lymphoma

In order to investigate the efficacy and safety of oral cilazapril, a new angiotensin converting enzyme inhibitor, on essential hypertension, a single daily dose of 2.5 to 5.0mg cilazapril was administered in 30 hypertensive patients with diastolic blood pressure in the range of 95??15mmHg while off all other anti-hypertensive agents for 10 weeks. Blood pressure and heart rate were measured every 2 weeks. The complete blood count with platelet count, blood chemistry by SMA-12 including lactic dehydrogenase and serum electrolytes, and urinalysis were performed at 4th and 10th week of therapy. The electrocardiography was performed at the beginning and the end of treatment period. Any kinds of side effects were actively questioned by the examining physicians. The following results were obtained : 1) The mean age was 49.2 years, and the ratio of male-to-female was 1 : 1.3. 2) Blood pressure started to fall significantly within 2 weeks of treatment with cliazpril 2.5mg(M+/-S.E., 15.4+/-17.4mmHg vs 138.5+/-23.3, 100.3+/-6.2 vs 89.4+/-6.6, p<0.05), and after 6 weeks of treatment with a mean dosage of 2.84mg. diastolic blood pressure of all subjects was maintained below 90mmHg throughout the rest of trial. 3) Pulse rate or body weight were not significantly changed during the entire treatment period(69.3+/-6.0/min vs 10th week : 69.0+/-7.7, 64.7+/-7.4kg vs 63.6+/-6.7, p>0.05). 4) There were no significant changes in blood chemistry including blood sugar, cholesterol and electrolytes, except mild changes of serum creativine and alkaline phosphatase values. 5) Hematologic findings, urinalysis and electrocardiographic findings remained unchanged. 6)Side effects were mostly mild in nature without potentially serious episodes(dry cough : 20%, indigestion, headache, dizziness, in order), but there was 1 cases in whom the dosage was redyced due to postural hypotension. From the above results, cilazapril with the dosage of 2.5 to 5.0mg was effectvie and well tolerated in essential hypertensive patients with diastolic blood pressure of 95 to 115mmHg, and cilazapril seems to be appropriate for monotherapy of mild to moderate hypertensive patients.
Alkaline Phosphatase ; Antihypertensive Agents ; Blood Cell Count ; Blood Glucose ; Blood Pressure ; Body Weight ; Chemistry ; Cholesterol ; Cilazapril* ; Cough ; Dizziness ; Dyspepsia ; Electrocardiography ; Electrolytes ; Headache ; Heart Rate ; Humans ; Hypertension ; Hypotension, Orthostatic ; Oxidoreductases ; Peptidyl-Dipeptidase A ; Platelet Count ; Urinalysis