OBJECTIVE: To investigate the effect of Compound danshen dripping pills on cytochrome CYP1A2 activity for reference of clinical rational drug use. METHODS: The contents of four major metabolites of Caffeine, i.e. 5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X), 1-methyluric acid(1U) and 1, 7-dimethyluricacid (17U) in the urine before and after oral administration of Compound danshen dripping pills were determined by RP-HPLC. The activity of cytochrome CYP1A2 was derived from the ratio of (AFMU+1X+1U)/17U. RESULTS: The average activity of CYP1A2 was 4.20?1.54 before medication, which stood at 4.26?1.95 at 14 days and 4.35?1.26 at 28 days after medication, up 1.42% and 3.57%, respectively, yet there was no significant difference after medication as compared with before medication. CONCLUSION: No significant difference was noted on CYP1A2 activity after oral medication of Compound danshen dripping pills, nor does it modify the efficacy of the concurrent drugs that metabolized by CYP1A2.

Objective To understand the status quo of health education requirement of cervical cancer patients undergoing chemotherapy, in order to take individualized health education to improve the quality of life of patients, as well as provide basis for hospitals to conduct targeted health education. Methods 68 cases of cervical cancer patients with chemotherapy were investigated with health education questionnaire. Results Cervical cancer patients with chemotherapy were in general needs of health education, to understand the treatment protocol and effect came to the first place of strong demand. Patients with different times of hospitalization, different age had different emphasis point for health education needs. The most popular education style was oral education. Conclusions Cervical cancer patients with chemotherapy lack knowledge of health education, they hope to obtain health education about examination, treatment and rehabilitation. Nurses should give targeted, planned and individualized health education in order to meet the health needs of patients and improve their quality of life.

Oral anticoagulants are important for preventing stroke in patients with atrial fibrillation.Compared with the traditional oral anticoagulant warfarin,the novel oral anticoagulants (NOACs) have the characteristics of high efficiency,safety,and no need to monitor coagulation function.However,current clinical reports have showed that the dose is usually low when NOACs were used for preventing stroke in patients with atrial fibrillation.Its main reason may be associated with the risk avoidance and the difference in doctor and patient preferences.The analysis from the aspects of safety and effectiveness,the risk of ischemic stroke or systemic embolism is lower when the dose of NOACs is relatively high,and the risk of hemorrhage is lower when the dose of NOACs is relatively lower.Given the differences between the incidences of different events,the disability rate and the mortality rate,the patient with atrial fibrillation are more suitable for using high-dose drug.When choosing a specific dose,taking into account the specificity,the appropriate dose,intensity,and dosing regimen should be given according to the guideline recommendations,appropriate reference to renal function and patient preferences,and individual differences in order to obtain the best clinical efficacy..

Objective To investigate the influence of CD146-siRNA on epithelial-mesenchymal transition in human ovarian cancer cell line A2780.Methods The influence of loss of CD146 on the mRNA and protein expressions of E-cadherin,vimentin,and fibronectin was evaluated by real-time polymerase chain reaction (RT-PCR) and Western blotting.Results Compared to non-transfected (control group) and those transfected with RNAi-NC (RNAi negative control group),the mRNA and protein expressions of Ecadherin were significantly increased in A2780 cell lines transfected with RNAi-CD146.The loss of CD146 expression down-regulated the mRNA and protein expression of vimentin and fibronectin(P ＜ 0.05).Conclusions CD146-siRNA can suppress epithelial-mesenchymal transition in human ovarian cancer cell line A2780.

Objective To investigate the rural medical orientation undergraduate student's professional commitment,time management disposition and learning burnout and the relationship between them. Methods Use the college students' learning Burnout scale,adolescence time management disposition Questionnaire and College Students'professional commitment scale,survey of Xinxiang Medical University grade 2010 ~2012,739 valid ques?tionnaires,the questionnaire has 92. 61% efficiency. Results Three scores were normally distribution. Learning burnout was the medium level,the average score of each entry was 2.88±0.45,and moderate and above level learn?ing burnout ratio was 45.06%. The average score of time management disposition was 3.45±0.43. Professional com?mitment level was higher,the average score was 3.82±0.43 for each item. Affective commitment,ideal commitment, the sense of time efficacy and the sense of time control have entered on the learning burnout of the stepwise regres?sion equation,and four variables together explained 44.0% of the variance in the dependent variable. Time manage?ment disposition,the professional commitment and learning burnout were negatively correlated, but between time management disposition and professional commitment is positively related, professional commitment, the variance explained by variables of learning burnout was 30.12%. Conclusion The rural medical orientation of undergradu?ate students time management disposition are important factors of learning burnout,which has the forecast function to the learning burnout. Professional commitment is part of the intermediary variables.

Objective To understand the epidemic situation,epidemiological characteristics and clinical features of import?ed malaria in Donghai County,Lianyungang City from 2005 to 2015. Methods The epidemiological data of imported malaria in Donghai County from 2005 to 2015 were collected and analyzed by the descriptive epidemiological method. Results A total of 126 imported malaria cases were reported in Donghai County from 2005 to 2015,including 101 falciparum malaria cases (80.19%),13 ovale malaria cases(10.32%),7 vivax malaria cases(5.56%),4 quartan malaria cases(3.17%),and 1 case with mixed infection(0.79%). These cases were distributed in 14 towns,and mainly in 4 towns namely Niushan,Tuofeng,Shil?iu,and Huangchuan,and the cases in the 4 towns accounted for 63.49% of the total cases. The cases occurred in the whole year,but the peak period of the disease was in April,which was related to the returned time of these patients from foreign coun?tries. All the patients were male and aged from 20 to 55 years,and those aged from 20 to 50 years accounted for 90.70%. Con?clusions The epidemic situation of imported malaria presents a rising trend in Donghai County in recent 10 years. To control imported malaria,the above?mentioned 4 towns with more migrant construction workers and businessmen should be treated as key areas. Meanwhile,the floating population management,disposal of the malaria epidemic spots,mosquito vector monitoring as well as health education should be strengthened.

Objective To investigate the diagnostic significance of combined detection of rheumatoid factor (RF),anti-cyclic cit-rullinated antibody (anti-CCP antibody),glucose-6-phosphatase (GPI)and anti-RA33 antibody in rheumatoid arthritis (RA).Meth-ods One hundred and twenty-six patients with RA,60 patients with other autoimmune diseases and 60 healthy subjects undergoing physical examination were selected as the research subjects.The singled detection of RF,anti-CCP antibody,GPI and anti-RA33 an-tibody,and their combined detection were used to analyze their roles in RA diagnosis.Results Compared with the healthy control group,the four indexes in the RA group were increased,the difference was statistically significant (P<0.05),the RF level in the autoimmune diseases group was increased compared with the control group,the difference was statistically significant(P<0.05). Compared with the the autoimmune diseases group,the four indexes in the RA group were increased,the difference was statistically significant (P<0.05).The sensitivity and specificity of RF to RA were 77.77% and 63.33%;the sensitivity and specificity of anti-CCP antibody to RA were 69.04% and 95.00%;the sensitivity and specificity of GPI antibody to RA were 25.40% and 100.00%;the sensitivity and specificity of anti-RA33 antibody were 27.77% and 99.16%,respectively.The specificity of combined 2 indica-tors was increased from 63.33% to 85.00%,which of combined 3 indicators was increased to 93.33% and which of combined 4 in-dicators even reached to 100.00%.Conclusion The combination of RF,anti-CCP antibody,GPI and anti-RA33 antibody indicators greatly increases with the sensitivity and specificity for diagnosing RA and has clinical significance.

Circulating tumor cell (CTC) is a kind of malignant tumor cells shed from the primary tumor into the circulation caused by spontaneity or surgical operation and puncture. With the development of CTC analysis recently, the detection methods have been gradually diversified. As a new biomarker, CTC plays a significant role in the diagnosis, therapeutic effect monitoring and prognosis of tumors. This paper mainly introduces the enrichment and analysis technology of CTC, and explore the relationship between CTC and tumors.

OBJECTIVE:To provide reference for rational use of antidepressant drugs in the clinic. METHODS:By DDD anal-ysis recommended by WHO,the utilization of antidepressant drugs in 34 hospital from Nanjing area during 2011-2014 were ana-lyzed statistically in respects of main type,consumption sum,DDDs and DDC,etc. RESULTS:The consumption sum and DDDs of antidepressants were increased year by year in 34 hospital from Nanjing area during 2011-2014,with the average annual growth rate of 18.21% and 19.25% respectively. The top 2 drugs by DDDs were paroxetine and setraline;DDC of tomoxetine,trazodone and dutoxetine took up the first 3 place;consumption sum of paroxetine,setraline,venlafaxine and escitalopram took up the first 4 places;consumption sum of paroxetine,mirtazapine and citalopram synchronized with their DDDs,and serial number ratio was close to or equal to 1.00. CONCLUSIONS:The use of antidepressant drugs in 34 hospitals from Nanjing area is rational in struc-ture and level;selective 5-hydroxytryptamine(5-HT)reuptake inhibitors(SSRI)and selective 5-HT noradrenaline reuptake inhibi-tor(SNRI)take up the predominant place;new type of antidepressant drugs are used widely and of great use potentiality in the fu-ture.

The pneumoperitoneum may reduce the renal blood flow which was demonstrated both in experimental and clinical research. Greater change could be seen as the pressure of pneumoperitoneum increased,which may induce lower GFR, decrease urine output ,increase the level of plasma Scr?BUN and so on.All these changes were related to the variety of hemodynamics?nerval-endocrine factors releasing?body position and sorts of insufflated gas.Ischemia-reperfusion injury also is an ignorable factor. Pneumoperitoneum may influence the kidney function , which is reversible under certain pressure and operating time,and were concerned with multiple mechanism. More obvious damage to the dysfunctional kidney could occur under high pressure of pneumoperitoneum.