Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Etanercept ; Female ; Humans ; Immunoglobulin G ; therapeutic use ; Male ; Methotrexate ; therapeutic use ; Phytotherapy ; Prednisone ; therapeutic use ; Receptors, Tumor Necrosis Factor ; therapeutic use

Objective　To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods　The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results　①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion　There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.

Cancer is one of the gene diseases. The genome stability is closely associated with the occurrence of cancers. XRCC1 gene is one of the DNA repair genes. It participates in the repair process when the genome is injured. So it is considered important in maintaining the stability of the genome and in the prevention of tumor occurrence. This article introduced the characteristic of XRCC1 gene, the relationship between single nucleotide polymorphism of the gene and susceptibility to tumor, and the common methods used in the XRCC1 research.

OBJECTIVE:To prepare rufloxacin hydrochloride gel and establish a quality control method of the gel METHODS:Carbopol940 was used as the gel base,the content of rufloxacin hydrochloride was determined with UV-spectrophotometry and the stability test was performed RESULTS:There was a good linearity of calibration curve of rufloxacin hydrochloride in range 2～10?g/ml,r=0 9 999,the average recovery was 99 53%,RSD was 0 38%(n=6) The gel was stable CONCLUSION:The design of the gel formulation is reasonable The gel can be prepared feasibly and is stable in quality,convenient in use

Cytochrome P450 enzyme are an important enzyme family in drug metabolism. Human cytochrome P450 enzymes have been studied entensively about their polymorphism in recent years. polymorphism of CYP2C19 and CYP2D6 have been detected at both phenotypic and genotypic level, and their molecular mechanism have been studied entensively, however, the molecular mechanism of other enzymes for exzample CYP2C9,CYP1A1,CYP2E1 et al) is being discussed, though these enzymes possiblely exist polymorphism. The article summarizes the substrates of P450, molucular mechanism of polymorphism, and paticular reference to the effects of this variation on drug metabolism and susceptibility to chemically-induced diseases.

Diastolic and systolic dysfunction of cerebral artery is an important pathophysioiogical basis of the occurrence and development of cerebrovascular diseases, and is also the presence of decreased cerebrovascular reserve capacity. Cerebrovascular myogenic and metabolic regulation abilities can be evaluated by different methods so as to reflect cerebrovascular reserve capacity, of those, transcranial Doppler ultrasonography is the most widely used one. Various diseases, such as hypertension, diabetes mellitus, and carotid atherosclerosis also have great effect on cerebrovascular reserve capacity.

Objective To analyze the clinical effects of dexmedetomidine on postoperative agitation in children patients . Method Forty-four children undergoing surgeries in our hospital between March 2011 and March 2014 were treated with intravenous injection of 10 mL dexmedetomidine with a dose of 0.5μg/kg in 15 minutes after general anesthesia. Result The occurrence rate of postoperative restlessness was only 6.82%(3/44). Conclusion The use of dexmedetomidine combined with specific clinical nursing intervention for children undergoing surgery can effectively reduce the occurrence of postoperative restlessness , which ensures the clinical therapeutic effect and life safety.

The effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the arachidonic acid(AA) metabolism were examined by incubating separately washed human platelets and human umbilical artery rings with labelled AA. The results are as follows:1.Both EPA and DHA reduced the formations of TXB2 in platelets and 6-keto-PGF1a in artery walls in dose-dependent way.2.The effect of EPA on the synthesis of HETE by platelets was different with the concentrations of EPA. At lower concentration (62.5?M), it increased the formation of HETE, but the degree of increase was lowerd when the concentration of EPA was increased further, and even reduced the formation of HETE when its concentration reached 1000?M although the decrease extent was statistically insignificant.3.At all of concentrations used but 62.5?M, DHA raised potently the formation of HETE by platelets in dose-dependent way.A brief discussion was made about our results with special references to former data reported.

PKHD1 ( polycystic kidney and hepatic disease 1), the causal gene of human autosomal recessive polycystic kidney disease(ARPKD), is located on chromosome 6p12.2 and covers a genomic region of ～500 kb. PKHD1 is among the largest human genes, with a minimum of 86 exons from which multiple transcripts may be generated by alternative splicing. The longest continuous open reading frame consists of 12 222 bp, encoding a 4 074 amino acid protein, designated as fibrocystin/polyductin (FPC). FPC is predicted to be a receptor like protein, with single transmembrane domain and a short cytoplasmic tail. The expression of mouse FPC can be detected in various duct-containing organs. In mouse embryogenesis, FPC appears in developing neural tube, bronchi, and the primordial gut as early as the day of E9.5. In fetal human kidneys, high level of FPC expression is present in ureteric bud and its expression continues throughout the process of renal tubuobranching. In adult human kidneys, FPC mainly expresses in the epithelia of renal collecting ducts. FPC is subcellularly localized to the primary cilia and concentrated on the basal bodies in renal epithelial cells. The detail function of FPC is still unrevealed, most recent studies demonstrate that FPC, as a receptor, may transduce cell signal by interacting with a trp superfamily TRPP2 (PKD2), which is a causal gene for ADPKD, and mediate intracellular calcium homeostasis to regulate differentiation, proliferation, migration and polarity of various duct/tubular epithelia, in turn, to modulate the formation of all physiologic ducts, tubules and tracts.

Early diagnosis of inflammation and properly interfere to improve the prognosis are very important.However,it is difficult to verify inflammation or differentiate inflammation from infection in clinic,especially in the condition of underlying inflammation.Currently,radiopharmaceuticals based on researches of pathophysiology progress of diseases are studied constantly.This review summarizes the potential inflammation imaging radiotracers and their mechanisms,potential clinical application value.