Aim To explore the role of macrophages in the progression of fibrosis in spontaneously hypertensive rats and study effects and possible mechanisms of benazepril.Methods 12-week-old male SHRs were divided into hypertension group(SHR,n=10),benazepril treatment groups(BenL,BenH subgroup;Rats received benazepril 1.7 or 17 mg?kg-1?d-1 intragastric administration,respectively;each n=10),and age and sex matched Wistar Kyoto rats were set as control group(WKY,n=10).After 18 weeks' intervention,systolic blood pressure(SBP),left ventricular mass(LVM),left ventricular mass index(LVMI),contents of left ventricular collagen and macrophages were examined.Transcriptional levels of TGF-?_1 and interleukin-6(IL-6)were evaluated by reverse transcription polymerase chain reaction(RT-PCR).Results The amount of macrophages in SHR group was strikingly increased and transcriptional levels of TGF-?_1 and IL-6 in SHR group were much higher when compared with WKY group(P

Myocardial fibrosis caused by various reasons (such as inf la mmatory, ischemia, hypertension) can induce the myocardial damage if the process continued. There is very progress in the research of the prevention and cure of myocardial fibrosis in present years. This paper summarizes the detection of my ocardial fibrosis in laboratory, and the progress and mechanisms of medication i n myocardial fibrosis.

Objective To analyze the relationship between the genetic polymorphisms of organic anion transporting polypeptide (SLCO1B1 and SLCO1B3) and mycophenolic acid ( MPA)pharmacokinetics in Chinese kidney transplant recipients. Methods Gene mutations (SLCO1B3T334G, SLCO1B1 A338G) were detected in 68 recipients by PCR-LDR. The plasma samples were collected and blood concentration of MPA was measured on the 28 th day after transplantation. The area under the curve (AUC)0-12 of MPA in different genotype recipients was compared to analyze the correlation between single nucleotide polymorphisms (SNPs) and MPA pharmacokinetics. Results MPA AUC0-12 was higher in SLCO1B3 T334G GG carriers group than in TT carriers [(54. 54 ±14.40)vs(37.30±12.88)mg·h·L-1,(P=0.052)].However,there was no difference in MPA AUC0-12 among each genotype of SLCO1B1 A338G (P>0. 05). Conclusion Genetic polymorphisms of SLCO1B3 affect interindividual variety in plasma MPA concentration in Chinese kidney transplantation recipients.

Genomic studies of cancer cell alterations,such as mutations,copy number variations(CNVs),and translocations,greatly promote our understanding of the genesis and development of cancers.However,the 3D genome architecture of cancers remains less studied due to the complexity of cancer genomes and technical difficulties.To explore the 3D genome structure in clin-ical lung cancer,we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer,combining with RNA sequenceing analysis.We demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells(1×104),compared the genome architecture between clinical samples and cell lines of lung cancer,and identified conserved and changed spatial chromatin structures between normal and cancer sam-ples.We also showed that Hi-C data can be used to infer CNVs and point mutations in cancer.By integrating those different types of cancer alterations,we showed significant associations between CNVs,3D genome,and gene expression.We propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin structures.Our study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysis pipeline for future larger-scale studies in lung cancer and other cancers.