1.Monitoring and its clinical significance of SP-D and interleukin-8 levels in bron-choalveolar lavage fluid of ACOS patients undergoing mechanical ventilation
Binfan YUAN ; Dong WU ; Ruoyun HE ; Yalian YUAN ; Bin WU
Chinese Journal of Immunology 2016;32(7):1035-1038
Objective:To investigate the concentration of pulmonary surfactant-associated protein D ( SP-D) and interleukin-8 (IL-8) in the bronchoalveolar lavage fluid (BALF) of ACOS patients undergoing mechanical ventilation (MV). Methods:38 patients with ACOS undergoing MV were involved in the study. According to the APACHEⅡscore, the patients were divided into the remission group (n=20) and the aggravation group (n=18). BALF was collected on the 1st and 5th days after MV. The content of IL-8 and SP-D in BALF was measured by ELISA. The levels of IL-8 and SP-D in BALF of the patients with different severity were compared. Results:The level of SP-D was significantly higher on the 5th day than on the 1st day in the remission group,and it decreased more ob-viously in the worsening group (P<0. 05). The level of IL-8 decreased gradually in the remission group but increased gradually in the worsening group. The level of SP-D was negatively correlated with IL-8 level and APACHEⅡ score. Conclusion:The findings suggest that the levels of SP-D and IL-8 in BALF are correlated with prognosis of ACOS patients undergoing MV,and it may prove to be useful as a guidance of therapy and prognosis.
2.Application of action-oriented teaching in senior midwifery practice teaching
Kaiyu DENG ; Xinxia YUAN ; Yufang GONG ; Bin WU
Chinese Journal of Medical Education Research 2014;13(4):390-393
Objective To explore the application effect of action-oriented teaching method in senior midwifery practice teaching.Methods Selecting 30 students from class 1,grade 2011 of the midwifery professional for the experimental group,another 30 students from class 2 for the control group.We selected four core contents for the experimental teaching refbrm such as obstetric abdominal examination,external pelvimetry measurement,prenatal vulva washing disinfection and sterile towel covering before the delivery.The experimental group was taught by action-oriented teaching,in accordance with the procedures:putting forward the projects or work task,set goals and grouping,making work plans,implementing and evaluating.The control group was taught by traditional teaching.We used questionnaire smvey and skills examination to evaluate the teaching effects and conducted teaching satisfaction scores and scores for t test to two groups of students.Results (1)Questionnaires:The resuhs of the 18 issues on the teaching effect evaluation,professional technology level and nursing soft skills,etc.showed that the experimental group's evaluation of the action-oriented teaching is higher than the control group's evaluation of the traditional teaching(P<0.05),action-oriented teaching is more helptul to improving the professional skill and nursing soft skills(P<0.05).@The scores of skill examination:The skills scores of the experimental group(80.01 ± 9.36) were significantly higher than those of the control group(71.32 ± 8.85)(t=3.70,P=0.000).The difference was statistically significant (t=3.70,P=0.00).Conclusions Action-oriented teaching helps to raise practice teaching effecr,train students' clinical work ability and nursing soft skills.It is suitable for practice teaching of senior midwifery.
4.Tegaserod can resume colonic electricity and motion of visceral hypersensitive rat.
Yuan-Jun YIN ; Bin LV ; Wu-Ming YANG
Chinese Journal of Applied Physiology 2008;24(2):174-183
Animals
;
Colon
;
physiology
;
Female
;
Indoles
;
pharmacology
;
Male
;
Myoelectric Complex, Migrating
;
Rats
;
Rats, Wistar
;
Viscera
;
physiology
5.Cell attachment of periodontal ligament cells on commercially pure titanium at the early stage.
Bin, ZHOU ; Yingguang, CAO ; Lijuan, WU ; Yanxiang, YUAN ; Yinping, ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(3):307-8, 310
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
Biocompatible Materials/pharmacology
;
Cell Adhesion
;
Cell-Matrix Junctions
;
Cells, Cultured
;
*Dental Implants
;
Periodontal Ligament/*cytology
;
Surface Properties
;
Time Factors
;
Titanium/*pharmacology
6.Effect of c-Jun N-terminal Kinase Inhibition on Gene Expression Induced by Interleukin-1 in Annulus Fibrosus Cells in Rats
Wei YUAN ; Yipeng WANG ; Zhihong WU ; Bin YU
Chinese Journal of Rehabilitation Theory and Practice 2013;19(4):341-345
Objective To determine the role of c-Jun N-terminal kinase (JNK) signaling in annulus fibrosus cells response to interleukin (IL)-1. Methods Rat annulus fibrosus cells cultured in monolayer were exposed to IL-1, with or without JNK inhibition by SP600125. RNA was isolated for Real-Time polymerase chain reaction (PCR) after 24 h stimulation. Results IL-1 upregulation of mRNA for inducible nitric oxide synthase (iNOS), IL-6, cyclooxygenase (Cox)-2, matrix metalloproteinase (MMP)-3, MMP-13 were blunted by JNK inhibition, while downregulation of collagenⅠand insulin-like growth factor (IGF)-1 were also reversed by JNK inhibition. Conclusion JNK signaling inhibition can be a therapeutic target for blocking intervertebral disc degeneration induced by IL-1.
7.Chemical shift assignments of two oleanane triterpenes from Euonymus hederaceus.
He-jiao HU ; Kui-wu WANG ; Bin WU ; Cui-rong SUN ; Yuan-jiang PAN
Journal of Zhejiang University. Science. B 2005;6(8):719-721
(1)H-NMR and (13)C-NMR assignments of 12-oleanene-3,11-dione (compound 1) were completely described for the first time through conventional 1D NMR and 2D shift-correlated NMR experiments using (1)H-(1)HCOSY, HMQC, HMBC techniques. Based on its NMR data, the assignments of 28-hydroxyolean-12-ene-3,11-dione (compound 2) were partially revised.
Euonymus
;
metabolism
;
Magnetic Resonance Spectroscopy
;
Molecular Conformation
;
Oleanolic Acid
;
analogs & derivatives
;
analysis
;
chemistry
;
Triterpenes
;
analysis
;
chemistry
9.Establishment and management of tissue biobank of osteoarthritis
Na WANG ; Danhui ZHAO ; Chengai WU ; Wei TIAN ; Bin XIAO ; Yue YUAN ; Yanzhuo ZHANG
Chinese Journal of Health Management 2012;06(4):235-238
Objective To establish tissue biobank of osteoarthritis in Beijing Jishuitan Hospital to promote orthopedic research study in China.Methods Fresh tissue or blood samples were collected from patients who underwent surgical operation for osteoarthritis since July 2007.Clinical information of the patientswasalsocollected.MicrosoftAccessdatabasesystemwasusedforthemanagementof information.Results From July 2007 to November 2011,a total of 2605 medical records and15188 tissue or blood samples were collected.Among them,165 tissue samples and 2005 blood samples were provided for molecular biology or epidemiological research.Conclusion Human tissue biobank is important for research work.Present osteoarthritis tissue collection and storage is feasible and could supply quality samples for study.
10.Correlation between cervical lesion development and histone acetylation modification that regulates RAR-β2 expression
Jiaojiao WU ; Dingqing FENG ; Yuan TIAN ; Hanjie XU ; Bing LI ; Bin LING
Chinese Journal of Clinical Oncology 2014;(5):300-304
Objective:To investigate the relationship between cervical lesion development and histone acetylation that regulates RAR-β2 expression. Methods:Immunohistochemistry and Western blot analysis were performed to detect AcH3, RAR-β2, and involu-crin expression in normal cervical tissues as well as in tissues with cervical intraepithelial neoplasia (CIN)Ⅱ-Ⅲand squamous cell cer-vical carcinoma. The relationship among histone acetylation level, RAR-β2 expression, and cervical lesion severity were analyzed. Re-sults:AcH3, RAR-β2, and involucrin expression were reduced or absent with the progression of cervical lesions;significant differences were noted between the groups (P<0.05). Histone acetylation level and RAR-β2 expression were positively correlated (r=0.797, P<0.05). AcH3 and RAR-β2 expression, which were both associated with the cervical lesions, were negatively correlated [r=-0.547(AcH3), r=-0.585(RAR-β2), P<0.05]. Conclusion:Histone acetylation modification is associated with the regulation of RAR-β2 expression. This pro-cess is also likely to participate in the carcinogenesis of cervical carcinoma.