1.In vitro antibacterial activity of WCM 302, a substance obtained from culture filtrate of streptomyces sp. 302, against staphylococcus aureus.
Woon Seob SHIN ; Joo Young PARK ; Choon Myung KOH
Journal of the Korean Society for Microbiology 1992;27(6):493-500
No abstract available.
Staphylococcus aureus*
;
Staphylococcus*
;
Streptomyces*
2.Optimum Culture Conditions for Production of Proteinases secreted by Candida albicans , C. tropicalis , and C. parapsilosis.
Choon Myung KOH ; Kyoung Ho LEE ; Woon Seob SHIN ; Joo Young PARK ; Hyun Sook PARK
Journal of the Korean Society for Microbiology 1997;32(4):421-428
In the present study, culture conditions to secrete proteinases from C. albicans, C. tropicalis and C. parapsilosis were examined. All three Candida species were found to secrete proteinases from acceleration phase to stationary phase, although the proteinase activities in culture filtrate were maximal during late exponential or early stationary phase. The proteinase activity in the culture filtrate of C. albicans cells grown at 30'C, was much higher than those grown at either 20 or 37'C. In culture of C. tropicalis and C. parapsilosis, the highest activity was found in culture filtrate grown at 37C. C. albicans secreted proteinases well in medium at initial pH 4.0-7.0. The optimal initial pH of medium for proteinase secretion was 7.0 for C. tropicalis and 5.0-6.0 for C. parapsilosis. All three Candida species secreted proteinases to greater amount in aerobic state. The most effective carbon source for proteinase secretion was xylose, glucose, maltose and sucrose for C. albicans, xylose for C. tropicalis and trehalose for C. parapsilosis. The effects of proteins, hydrolyzed proteins, ammonium sulfate as a sole nitrogen source on proteinase secretion were examined. Bovine serum albumin was the most effective nitrogen source of those tested and a little proteinase activity was detected in the culture filtrates when yeast cells were incubated in the medium containing ammonium sulfate. C. parapsilosis secreted proteinases to greater amount than the other Candida species in all nitrogen sources under study, indicating that C. parapsilosis proteinase would not be a inducible but a constitutive enzyme.
Acceleration
;
Ammonium Sulfate
;
Candida albicans*
;
Candida*
;
Carbon
;
Glucose
;
Hydrogen-Ion Concentration
;
Maltose
;
Nitrogen
;
Peptide Hydrolases*
;
Serum Albumin, Bovine
;
Sucrose
;
Trehalose
;
Xylose
;
Yeasts
3.Serum Indeces Protein Fibrils Expression in Candida albicans.
Choon Myung KOH ; Kyoung Ho LEE ; Woon Seob SHIN ; Dong Hwa KIM
Journal of the Korean Society for Microbiology 1999;34(3):277-283
The fibrillar coat of Candida albicans is of interest as its significance in antigenicity, antiphagocytosis, and adherence to host tissues. The partial biochemical properties and ultrastructure of fibrillar coat induced by rabbit sera were examined. The induced fibrillar layer was destroyed by treatments of lyticase, proteinase K and dithiothreitol. The total protein concentration of fibrillar cell wall lysate was higher than that of non-fibrillar cell wall lysate, but the total sugar concentration was similar. On SDS-PAGE analysis, the protein profiles between in fibrillar cells and in non-fibrillar cells were shown to be different. In fibrillar cells, the major bands of cell wall lysate were 83, 66, 54, 47, 33, and 26 kDa in dithiothreitol-treated lysate. The proteins of 26 and 19 kDa were predominant in lyticase-treated lysate. Although the fibrillar thickness and protein amount of cell wall lysate were increased in according to the incubation time, the protein profiles did not changed. These results suggest that the proteins of 83, 66, 54, 47, 33, 26, and 19 kDa may be major constituents of fibrillar coat in C. albicans.
Candida albicans*
;
Candida*
;
Cell Wall
;
Dithiothreitol
;
Electrophoresis, Polyacrylamide Gel
;
Endopeptidase K
4.Tumor cell growth inhibitory factor in the culture filtrate of the streptomyces 1. in vitro cytotoxicity of tumor cell growth inhibitory factor.
Soo Kie KIM ; Woon Seob SHIN ; Joo Young PARK ; Choon Myung KOH
Journal of the Korean Society for Microbiology 1991;26(4):375-387
No abstract available.
Streptomyces*
5.Antibacterial Activity of an Antibiotic (K-681) from Streptomyces sp. 681 against Staphylococcus aureus.
Choon Myung KOH ; Woon Seob SHIN ; Jung Bae KIM ; Dong Heui YI
Journal of the Korean Society for Microbiology 1998;33(1):69-75
The antimicrobial agents reduced infectious diseases significantly. However, antibiotic resistance has followed for almost every antimicrobial agent. Especially, Staphylococcus aureus was one of the most notorious for the multidrug resistance. Streptomyces sp. 681 has been selected for antibiotic-producing strain against methicillin-resistant Staphylococcus aureus (MRSA) from 1,000 strains of Actinomycetales which had been isolated from soil. In antimicrobial susceptibility test, all of the test strains were susceptible to vancomycin. However, most strains of Staphylococcus aureus were found to be resistant to methicillin. Ninety eight (75%) strains out of 129 strains showed multiple resistance pattern to more than 5 antimicrobial agents. The MIC values of the purified antibiotic (K-681) were 1-32 ug/ml against Gram-positive bacteria compared to >128 ug/ml against Grarn-negative bacteria or fungi. The MIC was 8 ug/ml for 90% of the 129 clinical isolates of S. aureus. The antibiotic showed no cytotoxicity against P 388, HeLa, and S180 at the concentration of 500 ug/ml.
Actinomycetales
;
Anti-Infective Agents
;
Bacteria
;
Communicable Diseases
;
Drug Resistance, Microbial
;
Drug Resistance, Multiple
;
Fungi
;
Gram-Positive Bacteria
;
Methicillin
;
Methicillin-Resistant Staphylococcus aureus
;
Soil
;
Staphylococcus aureus*
;
Staphylococcus*
;
Streptomyces*
;
Vancomycin
6.Potentiality of Anti-idiotypic Antibodies Mimicking GD2 to Induce Cellular Immunity.
Yoon Sun PARK ; Woon Seob SHIN
Immune Network 2004;4(4):229-236
BACKGROUND: Disialoganglioside GD2 is a tumor-associated antigen that is overexpressed on tumor cells of neuroectodermal origin, such as melanoma, small cell lung carcinoma and neuroblastoma. Immunity against GD2 has anti-tumor activities, but GD2 is poorly immunogenic. Anti-idiotypic antibodies that mimic GD2 may induce more effective immune responses than GD2 antigen itself, because they are protein antigens and are known to be able to break immune tolerance. In our previous study, we produced anti-idiotypic antibodies mimicking GD2 (3A4 and 3H9), which induced humoral immunity. However, cellular immunity is essential to eradicate tumor cells in vivo as well as humoral immunity. In the present study, we investigated whether these anti-idiotypic antibodies 3A4 and 3H9 could induce cellular immunes responses. METHODS: BALB/C mice were immunized with anti-idiotypic antibody 3A4 or 3H9, or normal mouse IgG as a negative control. Lymphoproliferative responses, cytokine production responses, and delayed-type hypersensitivity reactions were measured in mice immunized with the anti-idiotypic antibodies. RESULTS: Both the anti-idiotypic antibody 3A4 and 3H9 induced GD2-specific lymphoproliferative responses and IFN-gamma production of lymph node lymphocytes in BALB/C mice. Only anti-idiotypic antibody 3H9 induced significant GD2-specific delayed-type hypersensitivity in the mice. CONCLUSION: These results show that anti-idiotypic antibodies 3A4 and 3H9 have the potentiality of inducing GD2-specific cellular immune responses that cannot be induced by the native antigen GD2 itself.
Animals
;
Antibodies, Anti-Idiotypic*
;
Hypersensitivity
;
Immune Tolerance
;
Immunity, Cellular*
;
Immunity, Humoral
;
Immunoglobulin G
;
Lymph Nodes
;
Lymphocytes
;
Melanoma
;
Mice
;
Neural Plate
;
Neuroblastoma
;
Small Cell Lung Carcinoma
7.Detection of Antibody to Candida albicans Proteinase in Sera from C. albicans - Cultured Patients and Healthy Controls.
Kyoung Ho LEE ; Woon Seob SHIN ; Hyun Sook PARK ; Young UH ; In Ho JANG ; Kap Jun YOON ; Choon Myung KOH
Journal of the Korean Society for Microbiology 1997;32(3):307-314
To investigate whether anti-Candida proteinase antibody could be a diagnostic marker, we examined seroreactivity to proteinase in sera from 90 healthy controls and 8 of C. albicans culture-positive patients. Previously we purified proteinases of C. albicans, C. tropicalis, and C. parapsilosis using a series of chromatographic steps consisting of DEAE- Sepharose, Sephacryl S-200, and size-exclusion HPLC. ELISA and Western blot technique were adopted to examine seroreactivity of C. albicans proteinase with sera. On ELISA, the seroreactivities of healthy controls and C. albicans-cultured patients were 0.601 +- 0.014 (mean+SEM), and 0.695 +- 0.079, respectively (P=0.084, t-test). In C. albicans-cultured patients, the positive rate was 62.5% (5/8) and the positive rate of healthy controls was 39% (35/90). On Western blot analysis, C. albicans proteinase molecule was blotted by all sera tested. But the intensity of blotted band was different with the same dilution of sera; the intensity of C. albicans proteinase molecule band blotted by 2 sera of 3 healthy control's sera was distinctively lower than that by C. albicans-cultured patients sera. However, all sera including C. albicans-cultured patient's sera did not blot the proteinase secreted by C. tropicalis and C. parapsilosis. It is necessary to collect sequential sera of patients with candidiasis and to establish a cut-off value for ELISA or serum dilution for Western blot analysis that will give reliable test sensitivity and specificity.
Blotting, Western
;
Candida albicans*
;
Candida*
;
Candidiasis
;
Chromatography, High Pressure Liquid
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Peptide Hydrolases
;
Sensitivity and Specificity
;
Sepharose
8.Seroreactivities of proteinases of Candida albicans, C. tropicalis, and C. parapsilosis in sera from various Candida species-infected mice.
Kyoung Ho LEE ; Woon Seob SHIN ; Hyun Sook PARK ; Joo Young PARK ; Choon Myung KOH
Yonsei Medical Journal 1997;38(3):178-186
From the culture filtrates of C. albicans, C. tropicalis and C. parapsilosis, proteinases were purified using a series of chromatographic steps consisting of DEAE-Sepharose, Sephacryl S-200 and size-exclusion HPLC which removed contaminating mannoproteins and extraneous proteins. Anti-Candida proteinase antibodies in sera from mice infected with various Candida species were detected using ELISA for serodiagnosis of candidiasis. Three proteinases were blotted by homologous and heterologous anti-proteinase antisera on Western blot analysis. All sera from six Candida species-infected mice were reactive with proteinases of C. albicans, C. tropicalis, and C. parapsilosis, although C. glabrata, C. guilliermondii, and C. krusei did not secrete proteinase. The seroreactivities of proteinase with sera from mice infected with homologous C. albicans and C. tropicalis were higher than those with sera from heterologous Candida species-infected mice. These results suggest that three proteinases have at least one common epitope, but its application for diagnosis of candidiasis should be considered with limits of specificity.
Animal
;
Candida/genetics*
;
Candida/enzymology*
;
Candidiasis/enzymology*
;
Endopeptidases/analysis*
;
Female
;
Mice
;
Mice, Inbred ICR
;
Species Specificity
9.Ultrastructure of Fibrillar Layer of Candida albicans in Serum Culture.
Joo Young PARK ; Choon Myung KOH ; Woon Seob SHIN ; Dong Hwa KIM ; Kyoung Ho LEE ; Kyung Hoon KIM ; Yoon Sun PARK
Journal of Bacteriology and Virology 2001;31(2):105-112
No abstract available.
Candida albicans*
;
Candida*
10.A Case of Pyloric Obstruction Due to Gastric Tuberculosis.
Young Soo KIM ; Yong Woon SHIN ; Jin Woo LEE ; Dong Seob JANG ; Tai Hoon MOON ; Kee San SONG ; Seok JUNG ; Young Up CHO ; Young Chai CHU
Korean Journal of Gastrointestinal Endoscopy 1995;15(3):515-521
Gastric tuberculosis is a rare disease. It usually occurs secondarily to another lesions and mainly in the lungs. Only a few cases of primary gastric tuberculosis have been reported in the literature. Most commonly, gastric tuberculosis lesion locates in the lesser curvature side of the antrum. Therefore the clinical picture is similar to the peptic ulcer. A 24-year-old women visited to the Inha university hospital complaining of vomiting and epigastric discomfort. Gastrofiberscopy showed multiple polypoid mass around the pylorus with stenotic pyloric channel. Subtotal gastrectomy was performed and histologic examination revealed chronic granulomatous inflammation with caseation necrosis. That is compatible with tuberculosis. The patient was taken antituberculosis medication without complication. So we report the case of pyloric obstruction due to gastric tuberculosis with review of the literature.
Female
;
Gastrectomy
;
Humans
;
Inflammation
;
Lung
;
Necrosis
;
Peptic Ulcer
;
Pyloric Stenosis
;
Pylorus
;
Rare Diseases
;
Tuberculosis*
;
Vomiting
;
Young Adult