1.Antibacterial Activity of an Antibiotic (K-681) from Streptomyces sp. 681 against Staphylococcus aureus.
Choon Myung KOH ; Woon Seob SHIN ; Jung Bae KIM ; Dong Heui YI
Journal of the Korean Society for Microbiology 1998;33(1):69-75
The antimicrobial agents reduced infectious diseases significantly. However, antibiotic resistance has followed for almost every antimicrobial agent. Especially, Staphylococcus aureus was one of the most notorious for the multidrug resistance. Streptomyces sp. 681 has been selected for antibiotic-producing strain against methicillin-resistant Staphylococcus aureus (MRSA) from 1,000 strains of Actinomycetales which had been isolated from soil. In antimicrobial susceptibility test, all of the test strains were susceptible to vancomycin. However, most strains of Staphylococcus aureus were found to be resistant to methicillin. Ninety eight (75%) strains out of 129 strains showed multiple resistance pattern to more than 5 antimicrobial agents. The MIC values of the purified antibiotic (K-681) were 1-32 ug/ml against Gram-positive bacteria compared to >128 ug/ml against Grarn-negative bacteria or fungi. The MIC was 8 ug/ml for 90% of the 129 clinical isolates of S. aureus. The antibiotic showed no cytotoxicity against P 388, HeLa, and S180 at the concentration of 500 ug/ml.
Actinomycetales
;
Anti-Infective Agents
;
Bacteria
;
Communicable Diseases
;
Drug Resistance, Microbial
;
Drug Resistance, Multiple
;
Fungi
;
Gram-Positive Bacteria
;
Methicillin
;
Methicillin-Resistant Staphylococcus aureus
;
Soil
;
Staphylococcus aureus*
;
Staphylococcus*
;
Streptomyces*
;
Vancomycin
2.Species and antimicrobial susceptibility of enteropathogenic bacteria isolated in 1986-1991.
Kwang Soo PARK ; Kye Won LEE ; Dong Il WON ; Kyung Won LEE ; Woon Seob JUNG ; Oh Hun KWON
Korean Journal of Infectious Diseases 1993;25(3):221-229
No abstract available.
Bacteria*
3.A case of pulmonary epithelioid hemangioendothelioma.
Sang Hoon KIM ; Dae Jung SHIM ; Won Tae SEO ; Si Young LIM ; Seung Sei LEE ; Shin Ho KOOK ; Joo Seob KEUM ; Tae Yoon OH ; Woon Ha CHANG
Korean Journal of Medicine 1999;57(1):108-113
Epithelioid hemangioendothelioma(EH) is a rare vascular tumor of low-grade malignancy. It was previously described as intravascular bronchioloalveolar tumor (IVBAT). But electron microscopic study and immunohistochemical staining results have proved endothelial origin. Patients are usually asymptomatic and the tumors are found incidentally on routine chest X-rays. Most patients show a slowly progressive clinical course, even though some patients occasionally die as results of respiratory failure or extrathoracic complications. Confirmative diagnosis is made through thoracoscopic or open-lung biopsy. There is still no effective therapeutic modality for pulmonary EH. We have experienced a 55-year-old woman whose simple chest x-ray film revealed bilateral multiple small nodules. The nodules were histopathologically and immunohistochemically diagnosed as primary epithelioid hemangioendothelioma of the lung. The possibility of metastatic disease was excluded thorough clinical, laboratory and radiological studies. Comparison of radiographic chest film taken 3 years ago showed no significant progression of the pulmonary nodular lesions. Conservative management was instituted and careful 4 months follow-up showed no significant changes.
Biopsy
;
Diagnosis
;
Female
;
Follow-Up Studies
;
Hemangioendothelioma, Epithelioid*
;
Humans
;
Lung
;
Middle Aged
;
Respiratory Insufficiency
;
Thorax
;
X-Ray Film
4.A Case of Pyloric Obstruction Due to Gastric Tuberculosis.
Young Soo KIM ; Yong Woon SHIN ; Jin Woo LEE ; Dong Seob JANG ; Tai Hoon MOON ; Kee San SONG ; Seok JUNG ; Young Up CHO ; Young Chai CHU
Korean Journal of Gastrointestinal Endoscopy 1995;15(3):515-521
Gastric tuberculosis is a rare disease. It usually occurs secondarily to another lesions and mainly in the lungs. Only a few cases of primary gastric tuberculosis have been reported in the literature. Most commonly, gastric tuberculosis lesion locates in the lesser curvature side of the antrum. Therefore the clinical picture is similar to the peptic ulcer. A 24-year-old women visited to the Inha university hospital complaining of vomiting and epigastric discomfort. Gastrofiberscopy showed multiple polypoid mass around the pylorus with stenotic pyloric channel. Subtotal gastrectomy was performed and histologic examination revealed chronic granulomatous inflammation with caseation necrosis. That is compatible with tuberculosis. The patient was taken antituberculosis medication without complication. So we report the case of pyloric obstruction due to gastric tuberculosis with review of the literature.
Female
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Gastrectomy
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Humans
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Inflammation
;
Lung
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Necrosis
;
Peptic Ulcer
;
Pyloric Stenosis
;
Pylorus
;
Rare Diseases
;
Tuberculosis*
;
Vomiting
;
Young Adult
5.Different Responses of MSSA and MRSA to Oxacillin of Their Respective MICs.
Jong Soon JUNG ; Woon Seob SHIN ; Soo Ki KIM ; Yoon Sun PARK
Journal of Bacteriology and Virology 2009;39(4):287-294
All of the methicillin-resistant Staphylococcus aureus (MRSA) strains exhibit resistance to oxacillin by producing PBP2a encoded by mecA, whereas methicllin-susceptible Staphylococcus aureus (MSSA) strains do not. To investigate phenotypic differences other than oxacillin resistance level in responses to oxacillin between MSSA and MRSA, we compared alterations of viability and ultrastructure of MSSA by oxacillin treatment with those of MRSA. When MSSA and MRSA strains were exposed to oxacillin of their respective MICs, and then were assayed for viability and observed by transmission electron microscope, increase in thickness of cell wall was more prominent in MRSA strains than in MSSA strains, while decrease in number of surviving cells was more evident and change in morphology of growing cross wall was greater in MSSA strains than in MRSA strains. It is assumed that these different responses to oxacillin between MSSA and MRSA strains may be due to activation of some PBP2a unbound to oxacillin. In conclusion, MSSA and MRSA showed different functional and morphological responses to oxacillin, although they were treated with oxacillin of concentrations that respectively inhibit their proliferation.
Adenosine
;
Cell Wall
;
Electrons
;
Methicillin-Resistant Staphylococcus aureus
;
Oxacillin
;
Staphylococcus aureus
6.Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis.
Woon Seob SHIN ; Sun Ju CHOI ; Young Chul YANG ; Su Jung PARK ; Joo Young PARK ; Soo Ki KIM ; Kyoung Ho LEE
Korean Journal of Medical Mycology 2009;14(1):9-15
BACKGROUND: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients. OBJECTIVES: We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model. METHODS: Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain. RESULTS: The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups. CONCLUSION: These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.
Acrylic Resins
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Animals
;
Antibodies
;
Candida
;
Candida albicans
;
Cell Wall
;
Chromatography
;
Chromatography, High Pressure Liquid
;
Chromatography, Liquid
;
Chromatography, Reverse-Phase
;
Dithiothreitol
;
Endopeptidase K
;
Ethanolamines
;
Fungi
;
Glucan Endo-1,3-beta-D-Glucosidase
;
Immunization
;
Immunization, Passive
;
Mice
;
Multienzyme Complexes
;
Peptide Hydrolases
;
Phospholipases
;
Proteins
;
Survival Rate
7.Biological Properties of a Polyene Antifungal Substance(AF1) Produced from Streptomyces sp. WCM-9.
Woon Seob SHIN ; Seun ho JUNG ; Dong Heui YI ; Kyoung Ho LEE ; Soo Kie KIM ; Joo Young PARK ; Choon Myung KOH
Korean Journal of Medical Mycology 1996;1(1):47-54
BACKGROUND: Pathogenic fungi infect humans, especially immunocompromised patients, with superficial or deeply invasive pattern. In the past 20 years, fungal infections have been increased dramatically resulted by increment of organ transplantation, cancer, AIDS patients, or use of broad-spectrum antibacterial agents. Fungal infections are now important causes of morbidity and mortality of hospitalized patients. but there is no effective antifungal antibiotics as well as antibacterial antibiotics OBJECTIVE: Effective new antifungal antibiotics are needed for the treatment of mycosis. So in an effort to develop effective antifungal antibiotics, we screened over 600 isolates of Streptomyces sp. from soil. METHODS: Antifungal producing strain was selected using disk diffusion method, An antifungal substance (AF1) was purified with ethyl acetate extraction, silica gel column chromatography and reverse phase HPLC. MICs of AF1 were detected by agar dilition method. RESULTS: The compound showed UV maxima of 307, 321, 340, 359 nm indicating methylpentaene. Minimum inhibitory concentrations of the AF1 were 3.7 microgram/ml against mold, and 3.7 - 7.4 microgram/ml against Candida species. AFI was also active against Crytococcus neoformans, with MIC of 0.9 microgram/ml. The concentration of AF1 for K+ ion release from human red blood cell and hemolysis were 5 microgram/ml. CONCLUSION: The antibiotic purified from culture broth of Streptomyces sp. WCM-9 was a polyene antifungal antibiotic which have broad spectrum antifungal activity.
Agar
;
Anti-Bacterial Agents
;
Antifungal Agents
;
Candida
;
Chromatography
;
Chromatography, High Pressure Liquid
;
Diffusion
;
Erythrocytes
;
Fungi
;
Hemolysis
;
Humans
;
Immunocompromised Host
;
Microbial Sensitivity Tests
;
Mortality
;
Organ Transplantation
;
Silica Gel
;
Soil
;
Streptomyces*
;
Transplants
8.The Correlation Between Achilles Tendon Thickness and Cardiovascular Risk Factors.
Min Seob KWAK ; Se Jung YOON ; Yun Hyeong CHO ; Suk Min HONG ; Jong Kwan PARK ; Seungjin OH ; Dong Woon JEON ; Joo Young YANG
Journal of Lipid and Atherosclerosis 2013;2(2):77-83
BACKGROUND: Achilles tendon thickness (ATT) has been associated with increased cardiovascular risk in patients with familial hypercholesterolemia (FH). The aim of this study is to establish the correlation among ATT, obesity and established cardiovascular risk factors such as diabetes mellitus, hypertension, coronary artery disease, peripheral artery disease, smoking, and dyslipidemia. METHODS: In total, 19 patients (male 31.5%, mean age 60.0+/-12.5) with dyslipidemia and 96 control (male 64.6%, mean age 62.3+/-8.5) were enrolled. ATT was measured by ultrasonography. Anterioposterior diameter which represents the ATT was measured bilaterally, 4 cm above the insertion of Achilles tendon to the tuber calcite. Dyslipidemia was defined as elevated total cholesterol, triglyceride, or LDL cholesterol, or low levels of HDL cholesterol. RESULTS: There was no significant differences including ATT between the two groups (for ATT, dyslipidemia group, 0.44+/-0.04 vs control, 0.45+/-0.02 cm, p=0.783). There was no significant correlation between ATT and other cardiovascular risk factors except weight (r=0.34, p=0.007) and body mass index (r=0.63, p<0.001). Dyslipidemia was not significantly correlated with ATT (r=0.02, p=0.783). Use of statin was not significantly correlated with ATT (r=0.04, p=0.605). CONCLUSION: ATT was not significantly increased in patients with dyslipidemia. Lipid accumulation of Achilles tendon was not found in patients with dyslipidemia in this study.
Achilles Tendon*
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Atherosclerosis
;
Body Mass Index
;
Calcium Carbonate
;
Cholesterol
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Cholesterol, HDL
;
Cholesterol, LDL
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Coronary Artery Disease
;
Diabetes Mellitus
;
Dyslipidemias
;
Humans
;
Hydroxymethylglutaryl-CoA Reductase Inhibitors
;
Hyperlipoproteinemia Type II
;
Hypertension
;
Obesity
;
Peripheral Arterial Disease
;
Risk Factors*
;
Smoke
;
Smoking
;
Triglycerides
;
Ultrasonography
9.Extended Spectrum-beta-Lactamase or Carbapenemase Producing Bacteria Isolated from Patients with Acute Cholangitis.
Ja Chung GOO ; Mun Hyuk SEONG ; Young Kwang SHIM ; Hee Seung LEE ; Jung Ho HAN ; Kyeong Seob SHIN ; Jae Woon CHOI ; Sei Jin YOUN ; Seon Mee PARK
Clinical Endoscopy 2012;45(2):155-160
BACKGROUND/AIMS: This study assessed the antibiotic resistance organisms isolated from the blood and bile of acute cholangitis and evaluated risk factors associated with them and their impact on clinical outcomes. METHODS: The identities and antibiotic resistance profiles of bacteria isolated from 433 cases of acute cholangitis from 346 patients were analyzed. Risk factors and the outcomes of patients infected with them were assessed. RESULTS: Microorganisms were isolated from 266 of 419 blood cultures and 256 of 260 bile cultures. Isolates from bile and blood were identical in 71% of the cases. A total of 20 extended spectrum-beta-lactamase (ESBL)-producers and 4 carbapenemase-producing organisms were isolated from blood, and 34 ESBL-producers and 13 carbapenemase-producers were isolated from bile. Sixty-four (14.8%) cases were infected with any one of these bacteria isolated from blood or bile. Risk factors associated with them in blood were nosocomial infection and prior biliary intervention. In bile, indwelling biliary device was a risk factor associated with them. Antibiotic-resistant bacteria were associated with mortality, independent of other prognostic factors. CONCLUSIONS: ESBL or carbapenemase-producing bacteria were frequently isolated in acute cholangitis patients especially with prior biliary intervention and nosocomial infection. Isolation of antibiotic-resistant bacteria was an independent risk factor of mortality.
Bacteria
;
Bacterial Proteins
;
beta-Lactamases
;
Bile
;
Cholangitis
;
Cross Infection
;
Drug Resistance, Microbial
;
Humans
;
Risk Factors
10.Relationship between Biofilm Formation Ability and Virulence of Candida albicans.
Su Jung PARK ; Sun Ju CHOI ; Woon Seob SHIN ; Hyuk Min LEE ; Kkot Sil LEE ; Kyoung Ho LEE
Journal of Bacteriology and Virology 2009;39(2):119-124
Biofilms are microbial communities that form on a surface and are surrounded by extracellular polymeric substances. Candida biofilms are a cause of infections associated with medical devices. In the present study, an attempt was made to evaluate a significance of biofilm formation ability (BF) in virulence of C. albicans. C. albicans of 98 isolates, 24 commensal strains obtained from the oral cavities of healthy volunteers, 29 from blood culture, 25 from urine culture, and 20 from vaginal candidiasis, were assayed for BF, an ability to adhere to epithelial cells (ADH), cell surface hydrophobicity (CSH), and germ tube forming rate (GT). The relationships of BF with CSH, ADH, and GT were statistically examined. A positive correlation between BF and ADH was obtained, but the correlation (r=0.326) was relatively low. To assess BF as a factor contributing for candidiasis, mice lethality test was performed. The 10 isolates with the highest BF (mean survival rate, 24%) allow to kill mice more than those with the 10 lowest BF (mean survival rate, 47%). In addition, clinical strains isolated from blood culture, urine culture, and vaginal candidiasis showed higher BF than oral commensal strains. These results suggest BF may represent a virulent characteristic of C. albicans.
Animals
;
Biofilms
;
Candida
;
Candida albicans
;
Candidiasis
;
Epithelial Cells
;
Hydrophobic and Hydrophilic Interactions
;
Mice
;
Polymers
;
Survival Rate