No abstract available.
Pneumocephalus*

BACKGROUND: Herpes simplex virus thymidine kinase (HSVtk) phosphorylates the prodrug ganciclovir to a nucleoside analog that inhibits DNA synthesis, causing cell death. Neighbouring nontransfected cells may be affected through a 'bystander effect', thereby amplifying the antiproliferative actions. This study was carried out to determine whether retrovirus-mediated HSVtk gene therapy could reduce intimal hyperplasia and prevent stenosis following balloon injury of the rat carotid artery. METHODS: A replication-defective recombinant retroviral vector containing HSVtk cDNA (LtkSN) was constructed. Cultured primary rat smooth muscle cells (SMCs) infected with this vector (SMC/LtkSN) were transplanted to the balloon injured rat right carotid artery. One week after transplantation, HSVtk gene therapy group was administered a 2-week treatment of ganciclovir (30 mg/kg/d). Three weeks after balloon injury and SMC/LtkSN transplantation, carotid arteriography was performed and carotid arteries were perfusion-fixed for histologic examination. RESULTS: Carotid arteriographic evaluation comparing with the uninjured left carotid artery showed that the mean luminal diameter of HSVtk gene therapy group (n=5, 85+/-3%) was significantly larger than that of balloon injury only group (n=5, 65+/-5%). The neointimal mass of HSVtk gene therapy group was less than that of balloon injury only group. SMC/LtkSN transplantation without ganciclovir treatment group (n=3) showed asymmetric intimal proliferation probably because of gravitational pooling of seeding. There were inflammatory cell infiltrations at the gravity dependent portion of HSVtk gene therapy group. CONCLUSION: Retrovirus-mediated HSVtk gene therapy following balloon injury of the rat carotid artery reduced neointimal expansion and arteriographic stenosis.
Angiography ; Animals ; Carotid Arteries* ; Carotid Artery Injuries* ; Cell Death ; Constriction, Pathologic* ; DNA ; DNA, Complementary ; Ganciclovir ; Genetic Therapy* ; Gravitation ; Herpes Simplex* ; Hyperplasia ; Myocytes, Smooth Muscle ; Phenobarbital ; Phosphotransferases* ; Rats* ; Simplexvirus* ; Thymidine Kinase ; Zidovudine

There are 3 basic methods for surgical treatment of axillary osmidrosis; 1) method that removes only subcutaneous cellular tissue without removing skin 2) method that removes skin and subcutaneous cellular tissue en bloc, and 3) method that partially removes skin and subcutaneous cellular en bloc as well as removing the subcutaneous cellular tissue of the adjacent region. We studied the results of partial removal of the skin and subcutaneous cellular tissue en bloc, as well as the removal of subcutaneous cellular tissue of the adjacent region to compare the results of the bipedicled flap with the graft conversion method. There was no difference between two methods in results and complication rates. There are 3 advantage to this procedure. First, about 70-80% of apocrine glands were centrally distributed among the axillary hairbearing region therefore, resection of the central portion of axillary hair distribution area is important for good result. Second, the preservation of the subdermal plexus with careful excision of adjacent underlying subcutaneous tissue under the aid of the magnifying surgical loupe, is important for good wound healing. Third, the central excision of the axillary hair distribution area provides good exploration for undermining and defatting of the undersurface of the adjacent area, therefore it tooks a shorter operation time.
Apocrine Glands ; Hair ; Skin ; Subcutaneous Tissue ; Transplants ; Wound Healing

BACKGROUND AND SIGNIFICANCE: Because of the diverse clinical and radiologic manifestations of Neuro-Behcet disease, it is sometimes difficult to differentiate with multiple sclerosis or tumorous condition. We report two cases of Neuro-Behcet disease underwent by stereotaxic biopsy showing vasculitis without any evidence of malignancy, initially misdiagnosed as solitary tumor and metastatic tumor, respectively. CASE: A 35-year-old male admitted due to right hemiparesis. Brain CT showed low density mass shadow in left thalamus. Under the impression of low grade astrocytoma, stereotaxic biopsy was performed. Dense perivascular lymphocytic infiltration with petechial microhemorrhage mixed with hemosiderin pigment was noted in thalamic region. The other patient (male, 44yrs old) admitted due to seizure. On MRI, there are multiple inhomogenous signals on right occipital and left frontal lobe and left thalamus. Under the impression of metastatic tumor, work-up about the primary tumor was done without any positive findings. To determine the primary focus, stereotaxic biopsy was performed. Only reactive gliosis and mild perivascular lymphocytic infiltration was noted in pathologic specimen. With the retrospective careful history taking and follow-up image study, two patients were confirmed as having a Behcet's disease.
Adult ; Astrocytoma ; Biopsy ; Brain ; Follow-Up Studies ; Frontal Lobe ; Gliosis ; Hemosiderin ; Humans ; Magnetic Resonance Imaging ; Male ; Multiple Sclerosis ; Paresis ; Retrospective Studies ; Seizures ; Thalamus ; Vasculitis

No abstract available.
Blood Transfusion* ; Humans ; Infant, Newborn ; Infant, Premature*

This study was conducted to evaluate prevalence of intestinal helminthic infections and skin tests and Paragonimus and Clonorchis in tuberculosis(TB) patients in Korea. Stool examinations by formalin-ether technique and intradermal tests were carried out in 2 areas; Masan TB hospital and Kong-Ju TB hospital. The positive rate of intestinal helminths of any kind was 51.7 percent among 329 patients in Masan hospital, but 18.1 percent among 215 patients in Kong-Ju hospital. The infection rates of Trichocephalus trichiurus and Clonorchis sinensis were 20.7 percent and 17.6 percent respectively in Masan hospital patients, but the infection rates of T. trichiurus and C. sinensis were 6.5 percent and 6.0 percent respectively in Kong-Ju hospital patients. In skin tests, positive reactions to Paragonimus and Clonorchis antigens were 22.0 percent and 37.6 percent respectively in Masan hospital patients. On the contrary, Kong-Ju hospital patients showed the positive rates of 15.2 percent for Paragonimus and 27.2 percent for Clonorchis respectively.
parasitology-helminth-trematoda ; Ascaris lumbricoides ; Trichuris trichiura ; hookworm ; Trichostrongylus orientalis ; Clonorchis sinensis ; Paragonimus westermani ; Metagonimus yokogawai ; Taenis sp. ; Hymneolepis nana ; epidemiology ; skin test

Generally fabrication of neovascularized bone flap are produced by snadwiching vessels between bone segment with the optimal size and shape for the recipient sites. When these flaps are transferred to the recipient site, they are again isolated from the surrounding soft tissues, resulting not only decrease in the blood flow to bone segments by sacrifices of peripheral blood circulation due to elevation of flap, but also possible changes of shape of osseous flap by bone resorption. To overcome these disadvantage, it may be considerable to block bone segment off from the surrounding soft tissue. Purpose of this study are observed time-dependent changes of blood flow, vascularity and histologic finding of bone flaps using the autogenous bone and local vessel implantation. The results are compared with bone flap which had not been blocked off from surrounding soft tissues. Neovascularized bone flap were fabricated in 90 Sprague-Dawley rats using autogenous iliac bone and superficial inferior epigastric vessels. The experimental flaps were wrapped with silastic sheets to block neovascularization from surrounding soft tissues, and the control flaps were left unwrapped. The degree of bone formation, vascularity and blood flow were then assessed at 1, 2, 4, 6 and 8 weeks after flap fabrication, using a histological examination, microangiogram, corrosion cast study and radioactive microspheres. When fabricating a neovascularized bone flap using the autogenous iliac bone and the superficial inferior epigastric vessels, the physico-histological organization, vascularity and blood flow of neovascularized flaps were same or superior for wrapped than for unwrapped bone segments until the 4th week after grafting. This means that the period of neovascularization can be lengthened by the 4th weeks in the fabrication of bone segments, which can be blocked off from surrounding soft tissues. For these reasons, blocked bone flap fabrication can be considered to be superior to the conventional method.
Blood Circulation ; Bone Resorption ; Corrosion ; Microspheres ; Osteogenesis ; Rats, Sprague-Dawley ; Transplants

A total of 421 patients hospitalized in St. Mary's Hospital were examined by indirect latex agglutination test in order to evaluate the Toxoplasma antibody in Korean from June to August 1981. The test sera of the patients were obtained from each age group by random sampling. The 421 samples of test sera showed negative in 153, 1:2 in 157, 1:4 in 59, 1:8 in 27, 1:16 in 7, 1:32 in 9, 1:64 in 2, 1:128 in 4 and 1:256 in 3 cases, respectively. The positive rate of Toxoplasma antibody was 4.3 percent in this sample when indirect latex antibodies of 1:32 or higher were regarded as positive. The titers of positive Toxoplasma antibodies were increased by age.
parasitology-protozoa ; Toxoplasma gondii ; toxoplasmosis ; immunology ; diagnosis

In this study the authors examined the protein components at various stages of Clonorchis sinensis, and those of tegument and metabolite of adult Clonorchis by using SDS-polyacrylamide gel electrophoresis and immunodiffusion. The following results were obtained: The protein components of C. sinensis were gradually changed during its development. A considerable change occurred during the initial 7 days after the metacercarial infection. Two bands of protein of about 97,000 molecular weight (MW) and 178,000 MW were unique to excysted metacercaria of C. sinensis. Other 2 bands of protein of 23,000 and 25,000 MW which were absent in metacercariae, might be associated with the development of sex organs in adult. In the metabolite, some components of tegumental proteins were detected. And this tegumental protein components in metabolite seems to be the major antigenic components reacting with infected rabbit antiserum by immunodiffusion. Twenty bands of protein were detected in the isolated adult tegument. Among them 6 bands were in 97,000-65,000 MW, 3 bands in 56,000-53,000 MW and 5 bands in 37,000-30,000 MW. On the other hand, in metabolic products of adult C. sinensis, 17 bands were detected.
parasitology-helminth-trematoda ; Clonorchis sinensis ; biochemistry ; protein

For the purpose of making a comparative study of protein compositions in Ascaris suum by sexes and body parts, extracts were prepared from whole bodies, body walls, genital organs, digestive organs and body fluid, of both sexes. And electrophoretic analysis was conducted using polyacrylamide slab gel in the presence of sodium dodecyl sulfate. The results were as follows: In this study, protein bands of each part were separated in the largest number and most clearly under 8 -12 percent (10 percent) gradient gel condition. The number of bands by body parts was 43 in body walls, 51 in genital organs, 47 in digestive organs, and 34 in body fluid. When examined in terms of sex, the number of bands of whole body was 38 in females and 35 in males. The electrophoretic patterns of body wall protein were in most cases similar with those seen in digestive organs. And the band with a molecular weight of 72,900 was unique to the body wall, and the 122,000 MW band was unique to the female body wall. In genital organ extracts, large molecular weight proteins (more than 80,000) were more frequently met. The molecular weights showed some differences between the two sexes. Of the bands, those having molecular weights of 119,700, 100,500, 88,500 and 86,100 were unique to the female genital organs. On the other hand, the male genital organs showed unique bands having molecular weights of 87,100, 82,800, and 15,500. An unique band common to the genital organs of both sexes was one having 49,300 MW. In the digestive organs evenly distributed protein bands of molecular weights of l0,000-120,000 were observed. The band having 59,800 MW was unique to the digestive organs. The number of bands obtained from body fluid was comparatively small, and the number of bands having less than 30,000 MW was 7, accounting for 55 percent of the total protein amounts. The bands having 47,600 MW and 31,400 MW were unique to body fluid.
parasitology-helminth-nematoda ; Ascaris suum ; biochemistry ; protein