Search Results

1.Purification and Analysis of Amino Acid Sequences of Pulmonary Surfactant Proteins.

Journal of the Korean Society of Neonatology 1999;6(1):1-7

PURPOSE: For the synthesis of surfactant protein(SP) peptides and production of next generation artificial pulmonary surfactant(PS), we have isolated SP-B, C from bovine PS, and studied the biochemical properties and amino acid sequences of these protein-peptides. METHODS: Crude surfactant and purified surfactant were isolated from materials extracted from the bovine lung lavage. The hydrophobic SP-B, C were purified by Sephadex LH 60 column chromatography from PS. The purities of SP-B, C were assessed by tricine buffer SDS-polyacrylamide gel electrophoresis and the amino acid sequences of these proteins were determined using Beckman PI-2090. RESULTS: The molecular weights of SP-B, C shown in SDS-polyacrylamide gel electrophoresis were as follows; 15,000-18,000(oligomer) Da for SP-B, 3,500-5,000 Da for SP-C. The amino acid sequences were; FPIPLPYCWL LRTLIKKIQA VIPKGVLAMT VAQCHVVPL LVGGIQQLV IEYSVILLTD TLLGRLPNLV CGLRLRCSG in SP-B, LIPCCPVNIK RLLIVVVVVV LLVVVIVGAL LMGL in SP-C, respectively. These results indicated that the amino acid sequences of bovine SPs were different. CONCLUSION: The SP-B, C were purified from bovine PS, and amino acid sequences of SP-B, C were determined. Further studies are needed for the development and use of next generations of exogenous PS preparation based on synthetic SP-peptides for the treatment of neonatal RDS in the future.
Amino Acid Sequence* ; Bronchoalveolar Lavage ; Chromatography ; Electrophoresis ; Family Characteristics ; Humans ; Infant, Newborn ; Molecular Weight ; Peptides ; Pulmonary Surfactants*

2.Three Cases of Cardiac Injury due to Penetrationg and Blunt Trauma.

Myung Kyu PARK ; Jong Soo WOO ; Pill Joe CHOI ; Byung Woo BAE ; Yong Hoon LEE

Journal of the Korean Society of Emergency Medicine 1997;8(4):637-645

Three cases who were successfully treated for cardiac injuries by open thoracotomy were reported. The range of the ages of the patients was 3rd decade. Two of the 3 were males with blunt injuries by motor vehicle accident and one was male with stab wound by knife. In the case of blunt injuries, the right atrium was involved in one case and the right and left atrium were involved in the other. The site of stab wound was the right atrium. There were two cases with the pre-operative diagnosis of cardiac injuries and they were blunt injuries. The case of stab wound was not diagnosed pre-operatively. All cases were treated with open thoracotomy-one posterolateral thoracotomy, one median sternotomy and one Clam shell incision. All of them had an uneventfull post-operative course.
Bivalvia ; Diagnosis ; Heart Atria ; Humans ; Male ; Motor Vehicles ; Sternotomy ; Thoracotomy ; Wounds, Nonpenetrating ; Wounds, Stab

3.A case of salmonella peritonitis during continuous ambulatoryperitoneal dialysis.

You Kyoung LEE ; Tae Youn CHOI ; Won Bae KIM ; Duk Yong KANG ; Jun Hee WOO

Korean Journal of Clinical Pathology 1992;12(3):375-379

4.A case of salmonella peritonitis during continuous ambulatoryperitoneal dialysis.

You Kyoung LEE ; Tae Youn CHOI ; Won Bae KIM ; Duk Yong KANG ; Jun Hee WOO

Korean Journal of Clinical Pathology 1992;12(3):375-379

5.Acid-Base and Electrolyte Change Before and After Exchange Transfusion with ACD-B Blood in Hyperbilirubinemia of Newborn.

Ki Hong PARK ; Chong Woo BAE ; Yong Mook CHOI ; Chang Ill AHN ; Jung Kook LEE

Journal of the Korean Pediatric Society 1989;32(3):311-320

6.A clinical study on the idiopathic respiratory distress syndrome of the newborn: comparison of radiographic and clinical findings.

Byoung Yul LIM ; Chong Woo BAE ; Yong Mook CHOI ; Chang Il AHN ; Sun Wha LEE

Journal of the Korean Pediatric Society 1991;34(1):16-23

7.Clinico-Hematologic Observation of Neonatal Hemolytic Disease due to Minor Blood Group Isoimmunization.

Chong Woo BAE ; Yong Mook CHOI ; Chang Il AHN ; Hyun sok CHI

Journal of the Korean Pediatric Society 1987;30(5):504-509

8.A Case of Tsutsugamushi Disease.

Tae Kyu HAME ; Soo Chun KIM ; Chong Woo BAE ; Yong Mook CHOI ; Chang Il AHN

Journal of the Korean Pediatric Society 1988;31(8):1048-1053

9.A Study of Maternal IgG Anti-A, B in ABO Hemolytic Disease of Newborn: Relation between Antibody titer in Mother and Clinical Severity in Neonate.

Chong Woo BAE ; yong Mook CHOI ; Hang Il AHN ; Hyun Sook CHI

Journal of the Korean Pediatric Society 1988;31(8):999-1006

10.Three Cases of Hemolytic Diseases of Newborns due to Anti - E Antibody.

Hee Joo HONG ; Jee Hyun KIM ; Sun Hee CHOI ; Chong Woo BAE ; Yong Mook CHOI

Korean Journal of Perinatology 2001;12(2):163-167