1.Clinical study on necrotizing lymphadenitis.
Jee Yeon JANG ; Hyun Rim CHOI ; Jin Woo KIM
Journal of the Korean Academy of Family Medicine 1992;13(6):491-502
No abstract available.
Lymphadenitis*
2.Clinical analysis on newborn infants treated with mechanical ventilation.
Yeon Sim KIM ; Dae Ho CHOI ; Cheol Woo PARK ; Yeon Kyun OH
Journal of the Korean Pediatric Society 1991;34(10):1346-1355
No abstract available.
Humans
;
Infant, Newborn*
;
Respiration, Artificial*
3.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
4.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
5.Solitary Type of Glomus Tumor Developed in Multiple Sites: Report of 3 Cases.
Yeon Ho PARK ; Sung Woo CHOI ; Baik Kee CHO ; Won HOUH
Annals of Dermatology 1994;6(2):225-229
No abstract available.
Glomus Tumor*
6.A Study of Frequency, Indications and Complications on Peripartum Hysterectomy.
Gyu Hong CHOI ; Yoon Jin JUNG ; Hoo Yeon JUNG ; Ryok Ho RYU ; Woo Ha HAN
Korean Journal of Perinatology 1998;9(3):292-298
By means of hospital-based data over 8 years we sought to evaluate the clinical indications and incidence of emergency peripartum hysterectomy by demographic characteristic and reproduction history. From the obstetric record of all deliveries at Chung Goo Hospital between Jan. 1, 1990, and Nov. 31, 1997, we identified all women undergoing emergency cesarean hysterectomy, calculated incidence rates, conducted statistical tests of linear trends and heterogenety, and observed the clinical indicatons preceding the onset of this procedure. There were 16731 deliveries during this period, Cesarean hysterectomy was performed in 24 of 5993 cesarean sections(0.40%) and in 10 of 10738 vaginal deleveries(0.09%), so more frequently after cesarean section than vaginal delivery. The age of patients varied from 22 to 40 years old. The higher the age and the parity of patients, the higher incidence of cesarean hysterectomy was noted. The most common indication of cesarean hysterectomy was uterine atony(52.94%) followed by placental disorders(41.18%), uterine myoma with pregnancy(2.9%) and uterine rupture (2.9%). All patients who had hysterectomy received transfusion from 1 pint to 57 pints. The postoperative complications were bladder injury, febrile morbidity, disseminated intravascular coaguolopathy and wound disruption. There were three maternal deaths, the cause was disseminated intravascular coaguolopathy and amniotic embolism. The data identifiy uterine atony as the primary cause for gravid hysterctomy. The data also illustrated how the incidence of emergency peripartum hysterectomy increases significantly with increasing parity, especially when influenced by a current placenta previa or a prior cesarean section. Maternal morbidity remained high.
Adult
;
Cesarean Section
;
Embolism
;
Emergencies
;
Female
;
Humans
;
Hysterectomy*
;
Incidence
;
Leiomyoma
;
Maternal Death
;
Parity
;
Peripartum Period*
;
Placenta Previa
;
Postoperative Complications
;
Pregnancy
;
Reproduction
;
Urinary Bladder
;
Uterine Inertia
;
Uterine Rupture
;
Wounds and Injuries
7.A Case of Hypergammaglobulinemic Purpura of Waldenstrom.
Yeon Ho PARK ; Young Ho YOO ; Dong Won LEE ; Sung Woo CHOI ; Baik Kee CHO
Korean Journal of Dermatology 1994;32(5):911-915
Hypergammaglobulinemic purpura of Waldenstrom is a distinct syndrome consisting of recurrent episodes of purpura, elevated serum r-globulins, elevated erythrocyteed rnentation rate, and mild anemia. This disease has been divided into the prirnary type without an uncerlying disease, and the secondary type with a known underlying disease. We diagnosed a hypergammaglobulinemic purpura of Waldenstram in a 53-year-old woman who presented sudden onset of showers of purpuric macules and petechiae of 24 hours duration involving her lower extremities with a 10-rnonth history. She showed characteristic laboratory findings consistent with those of hypergammiglobulnemic purpura of Waldenstrorri and also showed positive results of antinuclear antibody and rheumatoid factor. We tried to find out if there we any associated underlying disases such as systemic lupus erythematosus or Sjogren synirorne through various tests but faile it find any. We concluded she showed a primary type of hypergarnmhglobulinemic purpura of Waldcnstrorn.
Anemia
;
Antibodies, Antinuclear
;
Erythrocytes
;
Female
;
Humans
;
Lower Extremity
;
Lupus Erythematosus, Systemic
;
Middle Aged
;
Purpura
;
Purpura, Hyperglobulinemic*
;
Rheumatoid Factor
8.Two cases of Krukenberg's tumor.
Sung Soo CHAI ; Hye Mi LEE ; Jung Yeon CHOI ; Myun Woo SHIN
Korean Journal of Obstetrics and Gynecology 1993;36(7):2746-2755
No abstract available.
Krukenberg Tumor*
9.A case of pure choriocarcinoma of the ovary.
Hye Mi LEE ; Sung Soo CHAI ; Jung Yeon CHOI ; Eun Hee PARK ; Myun Woo SHIN
Korean Journal of Obstetrics and Gynecology 1993;36(7):2741-2745
No abstract available.
Choriocarcinoma*
;
Female
;
Ovary*
;
Pregnancy
10.A Novel Fluoroscopic View for Positioning the AO Clavicle Hook Plate Decreases Its Associated in situ Complications.
Yoon Suk HYUN ; Gab Lae KIM ; Sang Min CHOI ; Woo Jin SHIN ; Dong Yeon SEO
Clinics in Shoulder and Elbow 2016;19(1):25-32
BACKGROUND: The goal of this study was to evaluate whether a modified fluoroscopic technique for positioning a hook plate affected the clinical results of treating Neer type II distal clavicle fractures and Rockwood type V acromioclavicular (AC) joint separations with this device. METHODS: The study was a retrospective consecutive case series with data analysis. Sixty-four patients with a Neer type II distal clavicle fracture or a Rockwood type V AC joint injury treated between March 2009 and June 2013 were divided into 2 groups: traditional fluoroscopic technique (traditional view, 31 patients) or modified fluoroscopic technique ('hook' view, 33 patients). A visual analogue scale (VAS) score, the modified University of California-Los Angeles (UCLA) shoulder scale score, and radiographic osteolysis were the main outcome measures. RESULTS: The traditional group included a significantly larger number of patients with acromial osteolysis than the hook view group: 23 patients (74.2%) vs. 11 patients (33.3%), respectively (p=0.01). Before plate removal, the hook group reported less pain and higher UCLA shoulder scale scores than the traditional group: average VAS score, 1.55 vs. 2.26, respectively; average UCLA score, 30.88 vs. 27.06, respectively. However, there was no significant difference after plate removal. CONCLUSIONS: The hook view allows more accurate bending of the hook plate around the contour of the acromion, resulting in decreased osteolysis, decreased pain, and better function with the plate in situ.
Acromioclavicular Joint
;
Acromion
;
Clavicle*
;
Dislocations
;
Humans
;
Joints
;
Osteolysis
;
Outcome Assessment (Health Care)
;
Retrospective Studies
;
Shoulder
;
Statistics as Topic