2.Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos.
Gook Jun AHN ; Byeong Chun LEE ; Woo Suk HWANG
Journal of Veterinary Science 2001;2(2):131-137
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows: control group (treatments with Ca2+, Mg2+ -free PBS+100 micro M EGTA), IP3 group (control+25 micro M IP3) and IP3+ ryanodine group (control+25 micro M IP3+10 mM ryanodine). In experiments using microinjection, no significant differences were observed between any of the developmental stages of the electroporation experiment. For electroporation, cleavage rates were significantly higher in the IP3+ryanodine group than in the IP3 or control group (85.6% vs 73.7% or 67.6%, respectively). In the subsequent stages of embryonic development, such as morula and blastocyst formation, the IP3 and ryanodine group exhibited significantly higher rates of morula fomation than the IP3 or control groups (40.6% vs 24.2% or 16.7%, respectively). Similarly, the rate of blastocyst formation in the IP3+ryanodine group was significantly higher than the control group (16.3% vs 6.9%) but did not differ significantly from the IP3 group (16.3% vs 9.5%). In nuclear transfer, activation was performed at 30 hpm by microinjection and elecroporation with 25 micro M IP3+ 10 mM ryanodine followed by 6-DMAP treatment. No significant differences were observed at any stage of embryonic development and none of the embryos activated by electroporation reached either the morula or blastocyst stage. However, 3.8% and 1.9% of embryos activated by microinjection sucessfully developed to the morula and blastocyst stages, respectively. In conclusion, activation treatments using IP3 and ryanodine are able to support the development of bovine parthenogenetic and reconstructed embryos.
Adenine/administration & dosage/*analogs & derivatives/pharmacology
;
Animals
;
Cattle/*embryology/physiology
;
Cell Fusion
;
Electroporation/veterinary
;
Embryonic and Fetal Development/*drug effects
;
Enzyme Inhibitors/administration & dosage/pharmacology
;
Female
;
Inositol 1,4,5-Trisphosphate/administration & dosage/*pharmacology
;
Microinjections/veterinary
;
Nuclear Transfer Techniques
;
Oocytes/drug effects/growth & development
;
Parthenogenesis/*drug effects
;
Protein Kinase Inhibitors
;
Ryanodine/administration & dosage/*pharmacology
;
Skin/cytology
3.MR Imaging Finding of Intracranial Metastatic Melanoma: Report of Two Cases.
Eui Jong KIM ; Woo Suk CHOI ; Hee Young HWANG ; Hyun Cheol KIM
Journal of the Korean Radiological Society 1995;33(5):713-716
MR finding of melanoma shows peculiar signal intensity based on paramagnetic effect of melanin or intratumoral hemorrhage, which displays decreased T1 and T2 relaxation times in many cases. Authors report MR findings in two cases of intracranial metastatic melanoma, which is pathologically confirmed by operation.
Hemorrhage
;
Magnetic Resonance Imaging*
;
Melanins
;
Melanoma*
;
Relaxation
4.A Case of Acquired Tufted Angioma.
Jae Young HWANG ; Jin Woo PARK ; Sung Yul LEE ; Jong Suk LEE ; Kyu Uang WHANG
Korean Journal of Dermatology 1999;37(1):128-130
Acquired tufted angioma is a benign, progressive vascular lesion that has a distinctive histopathologic appearance. We report a 31-year-old man with acquireed tufted angioma. Several reddish papules and plaque were present on the neck. The lesion had been present for 6 months. They had enlarged slowly, and were slightly tender. Histopathologic examination of the biopsy specimen showed round to ovoid cellular tufts of capillaries, most prominent in the middle to lower dermis.
Adult
;
Biopsy
;
Capillaries
;
Dermis
;
Hemangioma*
;
Humans
;
Neck
5.Chlamydial infection in women with tubal pregnancy.
Woo Sik LEE ; Dong Hoon HWANG ; Byung Suk LEE ; Ki Hyun PARK ; Chan Ho SONG
Korean Journal of Obstetrics and Gynecology 1993;36(4):534-542
No abstract available.
Female
;
Humans
;
Pregnancy
;
Pregnancy, Tubal*
6.Chlamydial infection in women with tubal pregnancy.
Woo Sik LEE ; Dong Hoon HWANG ; Byung Suk LEE ; Ki Hyun PARK ; Chan Ho SONG
Korean Journal of Obstetrics and Gynecology 1993;36(4):534-542
No abstract available.
Female
;
Humans
;
Pregnancy
;
Pregnancy, Tubal*
7.A Case of Left Atrial Myxoma in Childhood.
Heu Ran MIN ; Myoung Hee KOOK ; Yong Jong WOO ; Jae Suk MA ; Tai Ju HWANG
Journal of the Korean Pediatric Society 1988;31(2):263-270
No abstract available.
Myxoma*
8.MRI of osteonecrosis of the femoral head : Diagnostic value and histologic correlation.
Chang Soo KANG ; Byung Woo MN ; Sung Ha LEE ; Suk Young HWANG
The Journal of the Korean Orthopaedic Association 1993;28(2):527-539
No abstract available.
Head*
;
Magnetic Resonance Imaging*
;
Osteonecrosis*
9.A Case of a Choledochal Cyst with a Mucinous Cystadenoma of the Pancreas.
Suk Woo SON ; Seok Joo HAN ; Eui Ho HWANG
Journal of the Korean Surgical Society 1999;57(3):451-457
A choledochal cyst is a relatively common lesion of the biliary tract in pediatric patients and usually presents abdominal pain, jaundice, and abdominal mass. Its etiology is unknown, but the theory of pancreatobiliary reflux through anomalous pancreatobiliary duct union is widely accepted. A mucinous cystadenoma of the pancreas is very rare in children. To our knowledge, there is no literature reporting a case of a choledochal cyst with a mucinous cystadenoma of the pancreas. We report a case of a choledochal cyst combined with a mucinous cystadenoma of borderline malignancy at the head of the pancreas in a 5-year-old girl and review the literature.
Abdominal Pain
;
Biliary Tract
;
Child
;
Child, Preschool
;
Choledochal Cyst*
;
Cystadenocarcinoma
;
Cystadenoma, Mucinous*
;
Female
;
Head
;
Humans
;
Jaundice
;
Mucins*
;
Pancreas*
10.LPS Induced Osteonecrosis of the Femoral Head in Rabbits.
Hee Jung KANG ; Jae Suk CHANG ; Woo Chin CHO ; Key Yong KIM ; Kyu Hwang UM ; So Gu LEW
Journal of Korean Orthopaedic Research Society 2001;4(1):43-51
No Abstract Available.
Head*
;
Osteonecrosis*
;
Rabbits*