In order to investigate the main factor of pathogenicity in V. mimicus, we have studied the toxic effects of j3-hemolysin produced by V. mimicus. The purified hemolysin of V. mimicus was active erythrocytes from three animal species including mouse, rabbit and rat, but the hemolysin was most active against rabbit erythrocyte. The hemolysin lysed cultured cell and killed mouse. Rapid death of mouse was observed with rather small doses of the toxin. Intravenous injection of 20 mg of the purified toxin killed mice within 25 sec. The hemolysin also had a lethal effect on intraperitoneal injection into mice although less than on intravenous injection. Purified hemolysin injected rabbits had large morphological change in jejunum. In electron micrograph of thin sections of the human erythrocytes, cells were threated with the hemolysin at 37 C for 5 min., significant changes were not observed. But after 10 min., hemolysis was observed and after 60 min., complete degradation of human erythrocyte was observed.
Animals ; Cells, Cultured ; Erythrocytes ; Hemolysis ; Humans ; Injections, Intraperitoneal ; Injections, Intravenous ; Jejunum ; Mice ; Rabbits ; Rats ; Vibrio mimicus* ; Vibrio* ; Virulence

Vibrio mimicus, marine bacteria pathogenic for fish, can causes acute gastroenteritis in human. Iron limmited condition like in human body, may change the surface structure of V. mimicus. In this study we obse'rved the effect of iron limmited condition on outer membrane protein of V. mimicus. Ethylenediamine-di (O-hydroxy-phenylacetic) acid (EDDA), an iron chelator, delayed the time to reach expotential growth of V. mimicus in brain heart infusion medium from 3 hours to 20 hours. Outer membrane protein of V. mimicus-CON (cultured in BHI) and V. mimicus-EDDA (cultured in BHI contain EDDA) were seperated by 1% sarcosine from total cell envelop. SDS-PAGE of V. mimicus-EDDA and V. mimicus-CON showed similar protein profiles contain 37 kDa major protein but 86 and 90 kDa protein were induced differently. Immunological properties of above protein were determined by ELISA and western blotting. 86 kDa EDDA- specific OMP was induced in V. mimicus (isolate 96-1), V. parahaemolyticus (serotype 09), V. alginolyticus (isolate 95-1), E. coli (human isolate) and V. vulnificus ATCC 27562 in iron limmited condition.
Bacteria ; Blotting, Western ; Brain ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Gastroenteritis ; Heart ; Human Body ; Humans ; Iron* ; Membrane Proteins ; Membranes* ; Sarcosine ; Vibrio mimicus* ; Vibrio*

No Abstract available.
Cross Infection* ; Internet*

No abstract available.
Spermatozoa*

Two phages for the pathogenic V. alginolyticus were isolated from marine products. These 2 phages were examined temperature stability, pH stability, inactivation by UV irradiation, damage on restriction system of host cell, antibody production, structure protein analysis and western blotting assay. V. alginolyticus phages(VAPs) fomed plaques about 0.5 - 0.9mm in diameter and bands 50 - 60% in sucrose density gradient, VAPs were stable below 65'C, pH 5 - 10 and mostly inactivation by UV irradiation for 120sec. Latend period was 15 - 20 min. and burst size was 1.3 - 1.4 * 10 PFU/cell. Restriction system of V. alginolyticus isolated was mostly inactivated by 45C, 20min. heating. VAPs had 14 specific structural proteins and 5 proteins related to antibody production.
Antibody Formation ; Bacteriophages* ; Blotting, Western ; Coriolaceae ; Heating ; Hot Temperature ; Hydrogen-Ion Concentration ; Sucrose ; Vibrio alginolyticus* ; Vibrio*

Accurate impression is very important to achieve desirable prosthesis and there are many factors in taking a good impression. For example, types of impression material, types of impression tray, impression taking methods and so on. Recently individual tooth tray technique is accepted as obtaining good impression that can be applied to multiple abutment impression, heavy salivated patient, to minimize the effect of natural teeth' s undercuts and to reduce pain during cord packing procedures. The purpose of this study was to compare the accuracy according to materials and forms of the individual tooth tray which is clinically applied nowadays. Used materials in experiment were divided into 3 types (acrylic resin, Futar' occlusion, Blu-mousse) and forms were divided into 2 types (forming occlusal vent hole or not and forming marginal vent space or not). Stone master model from impression body and metal master model were measured by X-PLAN360d to compare occlusal surface discrepancy and marginal discrepancy. The results were as follows 1. In comparison of occlusal surface discrepancy and marginal discrepancy according to materials, groups with three materials showed no statistical difference. 2. In comparison of occlusal surface discrepancy and marginal discrepancy according to occlusal vent hole, groups with occlusal vent hole showed significantly less marginal discrepancy than groups with no occlusal vent hole(p<0.05). 3. In comparison of occlusal surface discrepancy and marginal discrepancy according to 0.5mm marginal-vent-space, groups with no 0.5mm-marginal-vent-space showed significantly less mar ginal discrepancy than groups with 0.5mm-marginal-vent-space(p<0.05). In summary, these results suggest that individual tooth tray made of 3 types of materials with occlusal vent hole and individual tooth tray made of acrylic resin with no marginal vent space showed good accuracy of impression. In addition, individual tooth tray which is made of bite registration materials may be more useful because of advantage in facility and timesaving aspect of fabrication.
Humans ; Jaw Relation Record ; Prostheses and Implants ; Tooth*

Accurate impression is very important to achieve desirable prosthesis and there are many factors in taking a good impression. For example, types of impression material, types of impression tray, impression taking methods and so on. Recently individual tooth tray technique is accepted as obtaining good impression that can be applied to multiple abutment impression, heavy salivated patient, to minimize the effect of natural teeth' s undercuts and to reduce pain during cord packing procedures. The purpose of this study was to compare the accuracy according to materials and forms of the individual tooth tray which is clinically applied nowadays. Used materials in experiment were divided into 3 types (acrylic resin, Futar' occlusion, Blu-mousse) and forms were divided into 2 types (forming occlusal vent hole or not and forming marginal vent space or not). Stone master model from impression body and metal master model were measured by X-PLAN360d to compare occlusal surface discrepancy and marginal discrepancy. The results were as follows 1. In comparison of occlusal surface discrepancy and marginal discrepancy according to materials, groups with three materials showed no statistical difference. 2. In comparison of occlusal surface discrepancy and marginal discrepancy according to occlusal vent hole, groups with occlusal vent hole showed significantly less marginal discrepancy than groups with no occlusal vent hole(p<0.05). 3. In comparison of occlusal surface discrepancy and marginal discrepancy according to 0.5mm marginal-vent-space, groups with no 0.5mm-marginal-vent-space showed significantly less mar ginal discrepancy than groups with 0.5mm-marginal-vent-space(p<0.05). In summary, these results suggest that individual tooth tray made of 3 types of materials with occlusal vent hole and individual tooth tray made of acrylic resin with no marginal vent space showed good accuracy of impression. In addition, individual tooth tray which is made of bite registration materials may be more useful because of advantage in facility and timesaving aspect of fabrication.
Humans ; Jaw Relation Record ; Prostheses and Implants ; Tooth*

Sirenomelia is a severe form of caudal regression syndrome that results in a fusion of the lower extremities, which is not compatible with life. A various spectrum of anomalies affecting primarily the musculoskeletal, genitourinary and gastrointestinal systems also can occur. This rare malformation has a reported incidence rate of approximately 1 in 60,000 births, with a range of 1 to 1 percent of all malformed infants. We experienced a sirenomelic case with combined anomalies of genitourinary, cardiovascular and gastrointestinal systems. Maternal obstetric history revealed oligohydramnios and intrauterine fetal growth retardation, and the baby was spontaneously delivered at 37 weeks of gestational age, but died I hour after birth.
Infant ; Male ; Female ; Humans ; Incidence

Inflammatory pseudotumor of the liver is a relatively rare entity, and frequently misdiagnosed as a malignant tumor. We report a case of inflammatory pseudotumor involving the liver in a 53year-old man. The liver function test and serum alpha-fetoprotein level were within normal range. His preoperative diagnosis was as hepatocellular carcinoma by radiologic studies, and ultrasonography guided fine needle aspiration cytology and biopsy were done but confirmative diagnosis of malignancy or pseudotumor was not given. Grossly a relatively well marginated reddish brown soft mass with focal hemorrhage, measuring 5.0 cm in the largest diameter, was noted in the left lobe of liver. Surrounding hepatic parenchyma was yellowish brown in color without cirrhosis. Microscopically the mass showed typical findings of inflammatory pseudotumor and the ing liver tissue revealed diffuse fatty change and moderate chronic inflammatory cell on in the portal areas.
Biopsy ; Carcinoma, Hepatocellular

This paper is studied for statistical anaIysis of 1181 cascs of positive V.D.R.L test among 24414 cases who were visited for annual Physical examination and in or outpatients in the Han Gang Sung Shim Hospital during 5 years and 7 months from January 1972 to July 1977. Clinical survey was carried out with 182 syphilitic patients in the Department of Dermatology, Han Gang Sung Shim Hospital during 19 months from January l976 to July 1977. The results were as follows; 1. Positive rate for V.D.R.L test was 4.8%, and there were statistically significant differences in annual incidence(P<0.01). Biologic false positive rate for V.D.R.L test was 26. 7%, and there were statistically aignificant differences in annual incidence(P<0.01). 2. Departmental analysis such as Dermatology, Urology, Physical Examination and others including Obstetrics and Gynecology, Internal Medicine, Pediatrics etc. were carried out, the results showed that there were statistically significant departmental differences in V.D.R.L positive rate, but no in biologic false positive rate. 3. Biologic false positive rate was high inversely proportional to dilution titer, and that was a statistically significant difference(P<0.01). 4. 182 had syphilis, of whom 106(58. 2%) were latent syphilis and 74(40. 7%) were primary and secondary syphilis. 5. 119(65. 4%) among 182 syphilitic patients were male, most of patients(87.8%) occured in age from 21 to 40 years in the both sex. But there no statistically significant differences in age distribution between males and females. 6. The most predilection site of chancre was coronary sulcus(54.5%). 7. The lesions of secondary syphilis were composed of 18 cases(25%) of condyloma latum, 17 cases(23. 6%) of roseolar syphilid, 17 cases of papulo-squamous syphilid, 13 cases of alopecia syphilitica, 1 case of pustular syphilid, and 6 cases of others. 8. Married patients were nearly two times than single. In women, married. patients were much more(80. 3%) than single, and there were statistically significatnt differences(P<0.01). 9. There were no significant differences in S.T.S positive rate of spouse between infectious and latent syphilis. 10. Occupation distribution of male sexual partners were composed of 35 cases(29. 4%) of waitress, 28 cases(23. 5%) of call-girl, 20 cases(16. 8%) of office-girl, 17 cases of housewife, 12 cases of out of work, 7 cases of student. 1I. For patients with primary, secondary, and latent syphilis, treatment consiste of benzathine penicillin 2. 4 million units intramuscularly on the first visit and repeated three penicillin preparations at 7 days intervals in most of patients.
Age Distribution ; Alopecia ; Chancre ; Dermatology ; Female ; Gynecology ; Humans ; Internal Medicine ; Male ; Obstetrics ; Occupations ; Outpatients ; Pediatrics ; Penicillin G Benzathine ; Penicillins ; Physical Examination ; Serologic Tests* ; Sexual Partners ; Spouses ; Syphilis* ; Syphilis, Cutaneous ; Syphilis, Latent ; Urology