1.Conjunctival Epithelial Cells Auto-Cultivated In Vivo on Human Amniotic Membrane in Rabbits.
Jung Min WOO ; Woo Jin JEUNG ; Woo Chan PARK
Journal of the Korean Ophthalmological Society 2006;47(5):812-817
PURPOSE: The purpose of this study was to investigate the phenotype of conjunctival epithelial cells auto-cultivated in vivo on human amniotic membrane (CIVAMs) in rabbits for ocular surface reconstruction. METHODS: A fornix based-conjunctival flap 8 mm in diameter was made in 12 eyes of rabbits. Amniotic membrane was implanted into the conjunctival defects. Rabbit conjunctival epithelial cells were cultivated in vivo on amniotic membrane for a week. A frozen section was made of the excised specimen. To investigate the phenotype of CIVAMs, Hematoxylin-eosin staining, immunohistochemical staining to anti-MUC5AC (monoclonal antibody to conjunctival goblet cell mucin), and transmission electron microscopy were performed. RESULTS In 11 of 12 eyes, conjunctival epithelial cells grew successfully on amniotic membrane. Light microscopy demonstrated two to three layers of cuboidal epithelial cells and two to three layers of stratified epithelial cells in CIVAMs. CIVAMs exhibited non-goblet epithelial differentiation as determined by immunohistochemistry to anti-MUC5AC. Transmission electron microscopy of CIVAMs showed fine structure similar to that of normal conjunctival epithelial cells. CONCLUSIONS: CIVAMs showed morphological findings similar to normal conjunctival epithelial cells and are expected to accomplish more rapid reconjunctivalization than simple amniotic membrane transplantation. We expect that CIVAMs will be adopted in treating conjunctival burn, symblepharon, conjunctiva-scleral ulcer, and filtering bleb leakage with conjunctival defects.
Amnion*
;
Blister
;
Burns
;
Epithelial Cells*
;
Frozen Sections
;
Goblet Cells
;
Humans*
;
Immunohistochemistry
;
Microscopy
;
Microscopy, Electron, Transmission
;
Phenotype
;
Rabbits*
;
Ulcer
2.Conjunctival Epithelial Cells Auto-Cultivated In Vivo on Human Amniotic Membrane in Rabbits.
Jung Min WOO ; Woo Jin JEUNG ; Woo Chan PARK
Journal of the Korean Ophthalmological Society 2006;47(5):812-817
PURPOSE: The purpose of this study was to investigate the phenotype of conjunctival epithelial cells auto-cultivated in vivo on human amniotic membrane (CIVAMs) in rabbits for ocular surface reconstruction. METHODS: A fornix based-conjunctival flap 8 mm in diameter was made in 12 eyes of rabbits. Amniotic membrane was implanted into the conjunctival defects. Rabbit conjunctival epithelial cells were cultivated in vivo on amniotic membrane for a week. A frozen section was made of the excised specimen. To investigate the phenotype of CIVAMs, Hematoxylin-eosin staining, immunohistochemical staining to anti-MUC5AC (monoclonal antibody to conjunctival goblet cell mucin), and transmission electron microscopy were performed. RESULTS In 11 of 12 eyes, conjunctival epithelial cells grew successfully on amniotic membrane. Light microscopy demonstrated two to three layers of cuboidal epithelial cells and two to three layers of stratified epithelial cells in CIVAMs. CIVAMs exhibited non-goblet epithelial differentiation as determined by immunohistochemistry to anti-MUC5AC. Transmission electron microscopy of CIVAMs showed fine structure similar to that of normal conjunctival epithelial cells. CONCLUSIONS: CIVAMs showed morphological findings similar to normal conjunctival epithelial cells and are expected to accomplish more rapid reconjunctivalization than simple amniotic membrane transplantation. We expect that CIVAMs will be adopted in treating conjunctival burn, symblepharon, conjunctiva-scleral ulcer, and filtering bleb leakage with conjunctival defects.
Amnion*
;
Blister
;
Burns
;
Epithelial Cells*
;
Frozen Sections
;
Goblet Cells
;
Humans*
;
Immunohistochemistry
;
Microscopy
;
Microscopy, Electron, Transmission
;
Phenotype
;
Rabbits*
;
Ulcer
3.The longevity of immunofluorescent antibody in the patientsconfirmed as Tsutsugamushi disease.
Jeung Hoon LEE ; Beom Jin SUNG ; Tae Young YOUN ; Woo Hyun CHANG
Korean Journal of Infectious Diseases 1991;23(1):19-23
No abstract available.
Longevity*
;
Scrub Typhus*
4.Effect of Focal and Grid Pattern PASCAL Photocoagulation Treatment in Diabetic Macular Edema.
Journal of the Korean Ophthalmological Society 2011;52(2):197-202
PURPOSE: To investigate the effect of focal and grid pattern PASCAL photocoagulation treatment in diabetic macular edema patients. METHODS: A total of 72 patients (75 eyes) who were diagnosed as diabetic macular edema were retrospectively analyzed. The patients were divided into 3 groups as follows: group 1 (31 patients, 31 eyes) received an intravitreal bevacizumab injection, group 2 (17 patients, 20 eyes) underwent focal and grid pattern PASCAL photocoagulation treatment, and group 3 (24 patients, 24 eyes) underwent focal and grid pattern argon photocoagulation treatment. Macular thickness and visual acuity (logMAR) of 75 eyes were compared among groups before and after treatments. RESULTS: Visual acuity improved in 13 eyes (65.0%) in group 1, 16 eyes (51.6%) in group 2, and in 10 eyes (41.6%) in group 3; however, there was no statistical significance. Macular thickness reduction occurred in 24 eyes (77.4%) in group 1 and 13 eyes (65.0%) in group 2 which were statistically significant decreases at only 1 month after treatment (p < 0.05). CONCLUSIONS: Focal and grid pattern PASCAL photocoagulation is a good therapeutic option for diabetic macular edema. Focal and grid pattern PASCAL photocoagulation is as effective as intravitreal bevacizumab injection or focal and grid pattern argon photocoagulation treatment.
Antibodies, Monoclonal, Humanized
;
Argon
;
Eye
;
Humans
;
Light Coagulation
;
Macular Edema
;
Retrospective Studies
;
Visual Acuity
;
Bevacizumab
5.Use of Temporalis Fascia for Frontalis Sling.
Woo Jin JEUNG ; Sung Chur MOON ; Hee Bae AHN
Journal of the Korean Ophthalmological Society 2001;42(11):1530-1534
PURPOSE: A wide variety of surgical materials have been used for blepharoptosis such as catgut, silicone, 4-0 nylon polyfilament cable-type ophthalmic suture (Supramid(R)), autogenous tendon (palmaris longus),banked sclera, dura mater, fascia lata, and temporalis fascia. The aim of this study was to evaluate the effect of frontalis suspensory surgery with temporalis fascia. METHODS: The authors studied 91 frontalis slings (71 patients) performed from 1993 to 1999 at our hospital for repair of blepharoptosis. Results with temporalis fascia (group A) as the suspensory material were compared to those with autogenous (group B) and preserved (group C) fascia lata. RESULTS: The success rates of group A (31 lids 21 patients), group B (28 lids 24 patients), group C (32lids 26 patients) were 80.6%, 75%, 78.1% respectively. But there was no statistically significant difference in each groups (P>0.05). CONCLUSIONS: We concluded that temporalis fascia for frontalis sling could be used as suspensory material successfully. Moreover, long term and further evaluations must be considered.
Blepharoptosis
;
Catgut
;
Dura Mater
;
Fascia Lata
;
Fascia*
;
Nylons
;
Sclera
;
Silicones
;
Sutures
;
Tendons
6.Free flap reconstructions for head and neck cancer.
Sang Dug JEUNG ; Young Jin KIM ; Tae Woo LEE ; Chang Sup SEONG ; Jin Suk BYUN ; June Sik PARK
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(6):1307-1314
No abstract available.
Free Tissue Flaps*
;
Head and Neck Neoplasms*
;
Head*
7.Changes of Contralateral Eyelid Position after Unilateral Blepharoptosis Repair.
Dong Min SHIN ; Woo Jin JEUNG ; Hee Bae AHN
Journal of the Korean Ophthalmological Society 2008;49(1):8-13
PURPOSE: To evaluate the effect of unilateral blepharoptosis repair on contralateral eyelid position. METHODS: The medical records of 39 patients who had undergone unilateral blepharoptosis repair were reviewed for preoperative and postoperative margin reflex distance (MRD). RESULTS: Of the 39 patients, 28 (71.8%) had congenital ptosis and 11 (28.2%) revealed acquired ptosis. Twenty-two (56.4%) patients were male and 17 (43.6%) were female. Ages ranged from 3 to 55 years (mean 15.3+/-20.1 years). After unilateral blepharoptosis repair, the postoperative mean (+/-SD) change in MRD of nonoperated eye was -0.3+/-0.7 mm at week one, -0.3+/-0.6 mm at three months, and -0.2+/-0.7 mm at six months. CONCLUSIONS: After unilateral blepharoptosis repair, 35.9% of patients had a decrease in contralateral eyelid height at postoperative 3 months and amount of decrease was 0.2+/-0.7 mm.
Blepharoptosis
;
Eye
;
Eyelids
;
Female
;
Humans
;
Male
;
Medical Records
;
Reflex
8.Topographic Measurement of the Optic Nerve Head with Heidelberg Retina Tomograph (HRT) in Normal Korean.
Dong Won LEE ; Woo Jin JEUNG ; Sae Heun RHO
Journal of the Korean Ophthalmological Society 1999;40(8):2224-2231
We analyzed the optic nerve head of 180 normal eyes (male 107 eyes,female 73 eyes) of Korean with Heidelberg Retina Tomograph (HRT), and obtained the mean value of each parameters and the difference between men and women with use of HRT. We also studied the distribution of the values of each parameter and influence of age on optic disc parameters in normal Korean. In addition, for the parameters of Rim/Disc area ratio, we measured the mean value in 6 divisions of optic nerve head. The range of each values was distributed widely and there were many variations in optic nerve head parameters. Height variation contour was statistically different between men and women. Cup volume and Maximal cup depth increased statistically with age. Further evaluation will be needed to analyze more accurate and highly reproducible analysis.
Female
;
Humans
;
Male
;
Optic Disk*
;
Optic Nerve*
;
Retina*
9.The Effect of 4 Rectus Muscle Anterior Displacement in Porous Polyethylene Orbital Implant Exposure.
Woo Jin JEUNG ; Dong Min SHIN ; Hee Bae AHN
Journal of the Korean Ophthalmological Society 2007;48(11):1554-1561
PURPOSE: To investigate the effects of anterior displacement of four rectus muscles in exposure of porous polyethylene orbital implant (Medpor(R)). CASE SUMMARY: This retrospective study reviewed 4 eyes with exposed orbital implant who underwent evisceration with porous polyethylene orbital implant (Medpor(R)). The technique involves dissection of conjunctiva and tenon's capsule and isolation of four rectus muscles from sclera. Unsure if each muscle was sutured to each other or if a group of muscles were sutured to another group (i.e. superior and inferior to medial and lateral). Posterior tenon's capsule was closed interruptedly and conjunctiva was closed continuously. Orbital implant exposure was occurred at 2~36 months later after evisceration and implant insertion. One eye was noted wound dehiscence 1 week after operation, then re-suture was done. Re-exposure was notified in 1 eye at postoperative 3 months, but it was small, then we just observed. In other 3 cases, exposure was not identified till last follow up. CONCLUSIONS: Four rectus muscle anterior displacement procedure may be simple and useful method in exposure of orbital implant.
Conjunctiva
;
Follow-Up Studies
;
Muscles
;
Orbit*
;
Orbital Implants*
;
Polyethylene*
;
Retrospective Studies
;
Sclera
;
Tenon Capsule
;
Wounds and Injuries
10.The Comparison of The Matrix Perimetry and Humphrey Standard Perimetry in Various Patients Group.
Hyun Chul CHEON ; Woo Jin JEUNG ; Sae Heun RHO
Journal of the Korean Ophthalmological Society 2007;48(5):678-685
PURPOSE: To study the correlation between the Humphrey standard automated perimetry (SAP) and the Humphrey Matrix Frequency Doubling Technology (FDT) perimetry and diagnostic usefulness of the Humphrey Matrix FDT perimetry. METHODS: This study included 170 eyes of 170 patients. The 170 eyes were classified into 50 normal eyes, 20 ocular hypertensive eyes, 50 glaucoma-suspect eyes and 50 glaucomatous eyes. All subjects were examined with the Humphrey Matrix perimetry and the Humphrey standard automated perimetry. We studied the correlations between mean deviation (MD) and pattern standard deviation(PSD) of the Humphrey SAP and those of the Humphrey Matrix perimetry, respectively, in each of the classified group. RESULTS: Overall, MD and PSD of the Humphrey SAP were significantly correlated with MD and PSD of the Humphrey Matrix perimetry, respectively. However, MD and PSD of the Humphrey Matrix perimetry were not showed a significant correlation in normal subjects (P>0.05). CONCLUSIONS: In this study, there were significant correlations between the MD and PSD of the SAP and those of the Humphrey Matrix perimetry in glaucoma, glaucoma-suspect and ocular hypertension. These finding suggests the Humphrey Matrix perimetry has performance characteristics similar to SAP and diagnostic value.
Glaucoma
;
Humans
;
Ocular Hypertension
;
Visual Field Tests*