To evaluate the morphologic changes of the renal arteries in the condition of high-salt diet, we scheduled the control group which fed routine animal diet added 0.06% of Sodium chloride, low-salt group which fed with 2.0% of sodium chloride, and high-Salt group which fed with 8.0% of sodium chloride. The experimental animals were sacrificed every two weed until 20 weeks of final experimental week. The results obtained were as follow; 1. Slight intimal thickening of the renal arteries is observed from 16th experimental week and continued the end of the experiment in the rats of control group. 2. In low-salt group slight intimal thickening of the renal arteries is observed from 12th experimental week and continued to the end of the experiment. 3. In high-salt group the intimal thickening began from 6th experimental week and its degree was hasten with week, and provoked moderate to high degree of lesion at the end of the experiment. Medial proliferation and degeneration of the intima and media, though their quality is mild, also associated at the end of the experiment.
Animals ; Diet* ; Rats* ; Renal Artery* ; Sodium Chloride

BACKGROUND: Disinfection is essential for the prevention of hospital infection. HICLO-S (Soosan environment Co., Yongin, Korea), one of the electrolyzed oxidized water was recently developed as a disinfectant. This is not costly and does not cause any clinical problems and environmental pollution. We evaluated the disinfective activity of HICLO-S against several clinical isolates of bacteria and yeasts. METHODS: 25 strains of bacteria and two strains of yeast were exposed to HICLO-S (30 & 50 ppm HICLO-S) disinfectant for the various periods (0.5, 1, 2, 4, 8, 10, 15, 20 minutes). After the exposure to HICLO-S disinfectant, 0.01 mL of mixture of microorganisms and HICLO-S was inoculated into brain-heart infusion broth or onto Sabouraud dextrose agar and incubated at 35degrees C for 48hours. RESULTS: All strains of bacteria and yeast were killed within 30 seconds after an exposure to 30 ppm of HICLO-S. All of three strains of spore forming Bacillus subtilis were killed within 4 minutes after an exposure to 30ppm of HICLO-S, but all of three strains were killed within 30 seconds in 50 ppm of HICLO-S. CONCLUSIONS: This study showed that HICLO-S disinfectant was effective for the disinfection of commonly isolated bacteria and yeast from hospital, but less effective against spore-forming bacteria. It may be recommended that HICLO-S should he used for the effective disinfection of skin, instruments and hospital floors.
Agar ; Bacillus subtilis ; Bacteria ; Cross Infection ; Disinfection ; Environmental Pollution ; Glucose ; Gyeonggi-do ; Skin ; Spores ; Water ; Yeasts

BACKGROUND: The risk of rubella infection is higher among hospital employees than others because of more exposure to rubella viruses. Because rubella infection during pregnancy can cause congenital rubella syndrome, special preventive measures should be taken for female employees in the hospital. METHODS: 309 employees in Soonchunhyang Kumi Hospital, aged from 18 to 45 years old, were included this study. Rubella IgG antibody was detected by the method of MEIA (microparticle enzyme immunoassay). And non-immunized employees were received by Wister RA 27/3 rubella vaccination and rechecked rubella antibody titers at 2 months later after vaccination. RESULTS: Among 309 subjects, 276(89.3%) were positive for rubella IgG antibody. The overall mean titer of rubella antibody was 72 IU/mL (SD: 67 IU/mL). The rubella antibody in titer decreased gradually with aging. The seroconversion rate after vaccination was 100%(28/28). CONCLUSIONS: The authors recommend that the employees susceptible to rubella or all employees in the hospital should be vaccinated for preventing rubella.
Aging ; Cross Infection* ; Female ; Gyeongsangbuk-do ; Humans ; Immunoglobulin G ; Middle Aged ; Pregnancy ; Rubella Syndrome, Congenital ; Rubella Vaccine ; Rubella virus* ; Rubella* ; Vaccination*

No abstract available.

The purpose of this study is to evaluate the change of mucosal immunity in gastric diseases. A quantative analysis of IgA and secretory component(SC) in gastric diseases by immunohistochemical method was performed in 110 specimens. The results are as follows: 1) In normal gastric mucosa, all of 10 cases revealed a negative reaction to antihuman SC but 4 cases were positive for IgA. 2) In chronic superficial gastritis and chronic atrophic gastritis with intestinal metaplasia, the metaplastic cells except for the goblet cells were positive for both IgA and SC. 3) The dysplastic cells were also positive for both IgA and SC, and the regenerating cells in ulcer as well. 4) All of the well differentiated or moderately well differentiated adenocarcinomas showed positive reactions to antihuman IgA and antihuman SC, and the intensity appeared to be stronger in the former. However, among 10 cases of poorly differentiated adenocarcinoma SC was not demonstrated in 5 cases, and no IgA was present in one case. In 10 cases of signet ring cell carcinoma, 6 cases revealed a negative reaction to antihuman IgA and 6 cases to antihuman SC. The above results suggest that the secretory immunity is not essential in normal gastric mucosa. The intestinal metaplasia in chronic gastritis is considered as an adaptive response to chronic inflammation. The degree of differentiation in adenocarcinoma may be related to the mucosal immunity.
Adenocarcinoma

The aim of this study was to clarify the role of Kupffer cells in the mechanism of endotoxin-induced liver injury. The study on fine structure of Kupffer cells was performed after the injection of endotoxin. The endotoxin(Escherichia soli lipopolysaccharide 026: B6, 1.5mg/100 g of body weight) was intraperitoneally injected in Sprague-Dewley rats. Animals were sacrificed at 1/4, 1/2, 1, 2, 4, 8, 16, 24, 72 and 120 hours after the injection of endotoxin. Livers were extirpated and processed to be examined by light and electron microscopy. The results obtained were summerized as follows: Early changes observed in liver after endotoxin injection included the increased number and hypertrophy of Kupffer cells, infiltration of neutrophils and presence of fibrin thrombi within the sinusoids. The coritinuous increase of the Kupffer cells in number with hypertrophy, congestion and infiltration of inflammatory cells within the sinusoids were observed. Hepatocytes showed* fatty change and occasional necrosis. At 72 hours the congestion decreased. At 120 hours the number of Kupffer cells was increased, but the morphology of Kupffer cells became similar to that of the control group. The numbers and sizes of primary and secondary lysosomes and amount of euchromatin of Kupffer cells increased. Swellings and increase in number of mitochondria, Golgi complex, smooth endoplasmic reticulum, rough endoplasmic reticulum were evident. Microthrombi were present within the sinusoids. The swelling of rough endoplasmic reticulum and mitochondria, decrease of glycogen particles, fatty change, hypoxic vacuoles, pyknotic nuclei and occasional necrosis were observed in hepatocytes. At 72 hours the number of secondary lysosomes in Kupffer cells decreased. At 120 hours the morphology of Kupffer cells became similar to that of the control group. According to these results, it was postulated that the endotoxin was initially taken up by pinocytosis into Kupffer cells and degraded in secondary lysosomes of activated Kupffer cells. Kupffer cells may play an important role in the defense mechanism of liver during endotoxemia. The dysfunction of Kupffer cells and ischemia by sinusoidal microthrombi may cause liver injury.
Animals ; Endoplasmic Reticulum, Rough ; Endoplasmic Reticulum, Smooth ; Endotoxemia ; Estrogens, Conjugated (USP) ; Euchromatin ; Fibrin ; Glycogen ; Golgi Apparatus ; Hepatocytes ; Hypertrophy ; Ischemia ; Kinetics* ; Kupffer Cells* ; Liver ; Lysosomes ; Microscopy, Electron ; Mitochondria ; Necrosis ; Neutrophils ; Pinocytosis ; Rats* ; Vacuoles

No abstract available.

Hepatic fibrosis was induced in Sprague-Dawley rate to evaluate the ultrastructural changes of fat-storing cells (Ito cells). For experimental induction of liver fibrosis, the rats were administered intraperitoneally with 0.5 ml of 50% Ccl4 solution per Kg body weight, twice weekly for 12 weeks. The rats were sacrificed every week. The liver tissues were examined under light and electron microscopes. And the immunohistochemical study of desmin was also performed. The results were summarized as follows: Light microscopic findings: The cellular infiltrations was inflammatory cells and Kupffer cells developed from 1 week after Ccl4 injection, and were the most severe in 4 weeks. The strong immunoreactivity for desmin was also evident in 4 weeks. The centrilobular necrosis and fibrosis developed from 2 weeks after injection, and the necrosis persisted until 8 weeks. The progress of fibrosis was accompanied by decreases in cellular infiltration and reactivity for desmin, and increased gradual nodular formation was also observed. The cirrhosis was developed after 10 weeks. Electron microscopic findings: An increase in number of fat-storing cells was observed from 1 week after injection. Transitional cells characterized by a depletion of lipid droplets and a hypertrophy of the rER appeared after 2 weeks. The number of transitional cells with abundant collagen fibers in the extracellular spaces increased in 4 weeks. With progression of fibrosis the number of fat-strong cells decreased and proliferating fibroblasts with dilated rER were observed. According to these results it was revealed that there was an apparent transition from fatstrong cells to transitional cells and to fibroblasts. These cells had a few similar characteristics and may belong to the same cell population. Thus it was suggested that fatstrong cells might play an important role in hepatic fibrosis.
Animals ; Body Weight ; Collagen ; Desmin ; Extracellular Space ; Fibroblasts ; Fibrosis* ; Hypertrophy ; Kupffer Cells ; Lipid Droplets ; Liver ; Liver Cirrhosis ; Necrosis ; Rats ; Rats, Sprague-Dawley

The authors developed a comfortable program for routine work of surgical pathology. We used IBM PC (80386) and Foxbase plus program. The main function of this program was automatic coding and concurrent surgical report printing. During gross printing, previous biopsy number and its diagnosis were automatically searched and printed below gross description. The reported data were stored during surgical report printing simultaneously, and thus the typist's workload became considerably reduced. Search for specific cases could be performed by patient's name, surgical number, hospital number, diagnostic code numbers (SNOMED code micro glossary), and certain disease entities on very short duration.
Biopsy ; Clinical Coding ; Diagnosis ; Pathology, Surgical*

Sclerosing hemangioma of the lung is uncommon benign neoplasm of uncertain histogenesis. Their radiologic appearance is relatively distinct and well defined. Recently we experienced 3 cases of sclerosing hemangioma of the lung in 54, 52, 51 years old women. The light microscopic findings are similar to the features reported by Liebow and Hubbell (1956).
Female ; Histiocytoma, Benign Fibrous* ; Humans ; Lung* ; Pulmonary Sclerosing Hemangioma