1.The Detection of Enterotoxin Gene from Bacteroides fragilis Isolates in Korea by Polymerase Chain Reaction.
Hee Bok OH ; Won Keun SEONG ; Kyung Won LEE ; Gyung Tae CHUNG ; Keong Sup SHIN
Journal of the Korean Society for Microbiology 1997;32(5):521-528
Bacteroides fragilis is a Gram negative nonsporulating anaerobic rod bacterium that makes up about 1 to 2% of the norrnal human colonic microflora. In 1984, Myer et al. reported that some strains of B. fragilis produce enterotoxin and cause diarrheal disease in cattle and human. Since then it has been termed enterotoxigenic B. fragilis (ETBF). In this study, we tried to detect enterotoxin gene from 37 B. fragilis strains, isolated in Korean patients, to confirm the existence of ETBF and usefulness of PCR as a rapid diagnosis method. By this method, we identified 9 ETBF strains and confirmed their pathogenesis by cytotoxicity test. No significant cross- reactivity with other anaerobes or aerobes was observed. Thus, the PCR method may be considered useful for the sensitive and rapid detection of anaerobic infections. And the entire amplified PCR mixture was ligated into a pT7Blue T-vector and transformed into E. coli. When the nucleotide sequences of cloned PCR products were compared with reported enterotoxin gene, pBF529 inserted DNA sequence was nearly in good agreement with it but pBF570 inserted DNA sequence showed some difference at nucleotide 270-300. A search for nucleotide sequence homologies revealed that pBF529 exhibited 99%, but pBF570 indicated only 90% identity with reported enterotoxin gene. According to these results, it was suggested that ETBF toxin can be differentiated into at least 2 subtypes.
Animals
;
Bacteroides fragilis*
;
Bacteroides*
;
Base Sequence
;
Cattle
;
Clone Cells
;
Colon
;
Diagnosis
;
Enterotoxins*
;
Humans
;
Korea*
;
Polymerase Chain Reaction*
2.Purification of the major allergens from Korean Dermatophagoides pteronyssinus and production of the recombinant antigens.
Kyung Sup KIM ; Sahng Wook PARK ; Jung Won PARK ; Chein Soo HONG ; Sang Hwan OH
Journal of Asthma, Allergy and Clinical Immunology 1999;19(1):91-102
BACKGROUND: Purified major allergens of house dust mite are essential for evaluation of the allergic mechanism in molecular basis and development of new modalities of immunemodulation. OBJECTIVE: In this study, we aimed to purif group 1 and group 2 allergens from Dermatophagoides pteronyssinus (Dp). In addition, cDNAs corresponding to Der pI and II in Korean Dp were isolated and recombinant Der p1 and Der pII were synthesized. MATERIALS AND and METHOD: Der pI allergen was purified by ammonium sulfate precipitation, anion -exchange column chromatography, and gel filtrat,ion chromatography. Der pII allergen was purified by anion exchange chromatography, gel filtration chromatography, and a preparative isoelectric focusing method. RESULTS: Eight hundred ug of Der pI and 50 ug of Der pII were obtained from 100 g of culture medium and 1 g of mite bodies, respectively. The purities of these allergens were confirmed by SDS PAGE and the strong reactivity to the patient sera was identified. In order to produce a recombinant allergens, poly(A) RNA from house dust mites were isolated and used for cDNA synthesis by RT PCR. The cDNA was inserted into prokaryotic expression vector and the vectors were transformed into E. coli. A little amount of recombinant Der pI protein was produced due to the low solubility, and 1.2 mg of recombinant Der pII was produced from 1 L of E. coli culture medium. The antigenicity of Der pI was relatively weak, however, Der pII showed a strong antigenicity. Amino acid sequence of the amplified cDNA deduced from DNA sequences of Der pII showed 6 different variants. The variation of amino acid sequences suggests the possibility of high incidence of mutation of Der pII protein. CONCLUSION: A simplified method for the purification of Der pI and Der pII was developed. Recombinant allergens will be useful for the diagnosis and treatment of allergy with lower costs.
Allergens*
;
Amino Acid Sequence
;
Ammonium Sulfate
;
Base Sequence
;
Chromatography
;
Chromatography, Gel
;
Dermatophagoides pteronyssinus*
;
Diagnosis
;
DNA, Complementary
;
Electrophoresis, Polyacrylamide Gel
;
Humans
;
Hypersensitivity
;
Incidence
;
Isoelectric Focusing
;
Mites
;
Polymerase Chain Reaction
;
Pyroglyphidae*
;
RNA, Messenger
;
Solubility
3.An experimental study on the effectiveness of local spasmolytic agents in microvascular vasospasm.
Jae Hoon KIM ; Won Suk OH ; Yong Bae KIM ; Soon Jae YANG ; Jong Sup PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(5):724-730
No abstract available.
4.Squamous carcinoma arising in benign cystic teratoma.
Chan YOON ; Man Chul PARK ; Yong Woo LEE ; Won Sup OH ; Hye Rim PARK
Korean Journal of Obstetrics and Gynecology 1993;36(7):2428-2434
No abstract available.
Carcinoma, Squamous Cell*
;
Teratoma*
5.Calculation of the Residual Blood Volume after Acute, Non-Ongoing Hemorrhage Using Serial Hematocrit Measurements and the Volume of Isotonic Fluid Infused: Theoretical Hypothesis Generating Study.
Journal of Korean Medical Science 2016;31(5):814-816
Fluid resuscitation, hemostasis, and transfusion is essential in care of hemorrhagic shock. Although estimation of the residual blood volume is crucial, the standard measuring methods are impractical or unsafe. Vital signs, central venous or pulmonary artery pressures are inaccurate. We hypothesized that the residual blood volume for acute, non-ongoing hemorrhage was calculable using serial hematocrit measurements and the volume of isotonic solution infused. Blood volume is the sum of volumes of red blood cells and plasma. For acute, non-ongoing hemorrhage, red blood cell volume would not change. A certain portion of the isotonic fluid would increase plasma volume. Mathematically, we suggest that the residual blood volume after acute, non-ongoing hemorrhage might be calculated as 0·25N/[(Hct1/Hct2)-1], where Hct1 and Hct2 are the initial and subsequent hematocrits, respectively, and N is the volume of isotonic solution infused. In vivo validation and modification is needed before clinical application of this model.
Blood Volume
;
Hematocrit
;
Humans
;
Isotonic Solutions/*therapeutic use
;
*Models, Theoretical
;
Shock, Hemorrhagic/*prevention & control/*therapy
6.A study of teenage obstetrics complications.
Hyun Kyung KIM ; Jae Chern SONG ; Man Chul PARK ; Yong Woo LEE ; Won Sup OH
Korean Journal of Obstetrics and Gynecology 1993;36(7):2117-2123
No abstract available.
Obstetrics*
8.Reconstruction of the lower leg with the reverse-pedicled anterior tibial flap: a case report.
Won Suk OH ; Yong Bae KIM ; Young Man LEE ; Soon Jae YANG ; Chong Sup PARK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1993;20(5):1100-1105
No abstract available.
Leg*
9.Clinical Implications of Pixel Values in PACS ( Picture Archiving and Communications System ) : A comparison with Dual energy X-ray Absorptiometry.
Chong Suh LEE ; Jong Sup SHIM ; Won Hwan OH ; Youn Soo PARK ; Ki Sun SUNG
The Journal of the Korean Orthopaedic Association 1997;32(6):1450-1457
PACS (Picture Archiving and Communications System) is a computer-based image storage and retrieval system that can store, recall and display medical images rapidly on high resolution workstations. The image acquisition system consists of direct digital interface to computed radiography (CR) system. We reviewed X-rays of 84 patients who had both studies of Dual energy X-ray Absorptiometry (DXA) and AP, lateral views of lumbar vertebrae between January and December of 1995. We tried to verify the clinical implication of pixel values on PACS for DXA by comparing pixel values and bone mineral density (BMD) of the 2nd, 3rd and 4th lumbar vertebrae. They were all female between 44 and 72 years old, average age of 59. Bone mineral density (BMD), represented in DXA of a total of 252 vertebrae were mostly in the range of between 0.228 (22%) and 1.318 (121%). Pixel values of the AP and lateral views of the 2nd, 3rd and 4th vertebrae in the PACS workstation were closely correlated to bone mineral density (p=0.0001). The values of the DXA (bone mineral density and T score) get comparatively lower as the pixel values get smaller. In conclusion, the pixel value on PACS interfaced to CR could be utilized as an easy, speedy and economical tool for screening of osteoporosis.
Absorptiometry, Photon*
;
Aged
;
Bone Density
;
Female
;
Humans
;
Lumbar Vertebrae
;
Mass Screening
;
Osteoporosis
;
Radiography
;
Spine
10.Clinical Results of Open versus Endoscopic Carpal Tunnel Release.
Min Jong PARK ; Ki Sun SUNG ; Won Hwan OH ; Jong Sup SHIM
The Journal of the Korean Orthopaedic Association 1998;33(2):405-410
Open carpal tunnel release has been the standard method of sumical treatment of carpal tunnel syndrome. Recently endoscopic carpal tunnel release has been introduced and is heing used by many authors. The advantages of this new technique are less postoperative pain, rapid restoration of power and rapid return-to-work. However many considerate authors, in spite of these advantages. insist that the inevitahle risk of neurovascular injury during the endoscopic procedure should not he underestimated. The purpose of our study is to compare the clinical results of endoscopic carpal tunnel release with those of open release. 20 open carpal tunnel releases in 16 patients and 15 endoscopic carpal tunnel reieases (single-portal technique) in 11 patients were performed hy the first author. Preoperative conditions of both groups are not different. Authors compared the clinical results between the two groups with some parameters. The overall clinical results were not different significantly hetween two groups. Rapid return-to-work(36 days in endoscopic group versus 60 days in open group) and less postoperative scar and pillar pain in endoscopic group were demonstrated. However, the major complication of one median nerve injury in endoscopic group seemed to overweigh these some benefits. We suggest that the standard operative technique for carpal tunnel syndrome should be open carpal tunnel release and more considerations should be takcn in choosing endoscopic method because of its inherent risk.
Carpal Tunnel Syndrome
;
Cicatrix
;
Humans
;
Median Nerve
;
Pain, Postoperative
;
Return to Work