No abstract available.
Malaria*

No abstract available.
Flow Cytometry* ; Humans* ; Leukocytes* ; Microscopy* ; Phagocytosis* ; Staphylococcus aureus* ; Staphylococcus*

No abstract available.
Flow Cytometry* ; Humans* ; Leukocytes* ; Microscopy* ; Phagocytosis* ; Staphylococcus aureus* ; Staphylococcus*

No abstract available.
Peritoneal Dialysis*

We report a hemolytic transfusion reaction with acute intravascular hemolysis due to anti-Jkb in a 49-year-old woman with uterine myoma. A patient experienced chills, fever, and red color urine following the transfusion of 1.25 units of packed red cells, shown to be compatible by the conventional cross-matching tube method. She had been received two units transfusion 3 weeks ago and there was no transfusion reaction at that time. One day after transfusion, her laboratory data showed total bilirubin 2.7 mg/dL, LDH 2,310 IU/L, and a trace positive direct antiglobulin test. Irregular antibody screening test was negative by the conventional tube methods but anti-Jkb was identified by column agglutination method. The presence of anti-Jkb provided an explanation for the acute hemolytic reaction. The hemolytic transfusion reaction was secondary responses following the previous transfusion. She showed severe hemoglobinuria, but renal failure did not develop and she was fully recovered with maintaining adequate renal output with IV diuretics.
Agglutination ; Bilirubin ; Blood Group Incompatibility* ; Chills ; Coombs Test ; Diuretics ; Female ; Fever ; Hemoglobinuria ; Hemolysis ; Humans ; Leiomyoma ; Mass Screening ; Middle Aged ; Renal Insufficiency

Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.
Animals ; Blastocyst ; Cell Line* ; Epithelium* ; Fibroblasts ; Gene Expression ; Hypothalamus ; Oxytocin ; Plastics ; Polymerase Chain Reaction ; Rats* ; Stromal Cells ; Uterus

We report a case of a 72-year-old woman with Churg-Strauss syndrome, who presented with intestinal perforation. She has had bronchial asthma with peripheral blood eosinophilia for 30 years. Gross findings of a resected colon showed multiple ulcers with perforation. Histologic findings demonstrated transmural inflammation infiltrated with large numbers of eosionophils, neutrophils and lymphoplasma cells, and characteristic extravascular granuloma in the subserosa. There were multifocally-distributed transmural vasculitis showing all stages of activity in medium and small-sized arteries and veins located in the submucosa, and proper muscle and subserosal layers of the colon, some of which revealed granulomatous inflammation. Histologic finding of liver showed chronic viral hepatitis B with mild inflammatory activity and macronodular cirrhosis. Immunohistochemical findings, acid fuschin orange G staining and electromicroscope found no evidence of hepatitis B virus infection contributing to the pathogenesis of this lesion.
Aged ; Case Report ; Churg-Strauss Syndrome/virology ; Churg-Strauss Syndrome/pathology ; Churg-Strauss Syndrome/complications* ; Colon/virology ; Colon/pathology* ; Colonic Diseases/virology ; Colonic Diseases/pathology ; Colonic Diseases/etiology* ; Female ; Hepatitis B/pathology ; Hepatitis B Antigens/analysis ; Human ; Immunohistochemistry ; Intestinal Perforation/virology ; Intestinal Perforation/pathology ; Intestinal Perforation/etiology*

OBJECTIVES: Accurate assessment of the retained or recurrent bile duct stone is important in postcholecystectomy patients who have signs of cholangitis or other pancreatobiliary disease. This study was performed to evaluate the diagnostic and therapeutic reliability of endoscopic retrograde cholangiography in postcholecystectomy patients. METHODS: From October 1975 through August 1995, we reviewed 311 patients retrospectively who had undergone on cholecystectomy. Our study was based on assessment of their age, sex, symptoms and physical findings, comparison between sonographic and endoscopic retrograde cholangiographic findings, and stone removal rate using endoscopic sphincterotomy, basket, electro-hydraulic lithotripsy. RESULTS: The ratio of male to female was 1:1.67, and the mean age was 56+/-14.3 years. After cholecystectomy, they visited our clinic within 2 years in 25 patients(8.4%), beyond 2 years in 286 patients(91.6%). Most common symptoms and physical findings were epigastric pain or tenderness(63.6%). We detected bile duct stone using endoscopic retrograde cholangiography 219 patients. In case of the bile duct stone, we successfully removed it using endoscopic sphincterotomy, basket, electrohydraulic lithotripsy in 194 patients(88.6%). CONCLUSION: From the above results, we concluded that endoscopic retrograde cholangiography was the most effective diagnostic and therapeutic method in postch- olecystetomy patients who have signs of cholangitis.
Bile Ducts ; Cholangiography* ; Cholangiopancreatography, Endoscopic Retrograde ; Cholangitis ; Cholecystectomy ; Female ; Humans ; Lithotripsy ; Male ; Retrospective Studies ; Sphincterotomy, Endoscopic ; Ultrasonography

Somatostatinoma is a rare form of neuroendocrine tumor that was first described in 1977. Most tumors have involved the pancreas, and gastrointestinal tract involvement is rare. Somatostatinomas of the ampulla of Vater are extremely rare and present distinct clinical and pathologic differences. Pancreatic somatostatinoma has been associated with a clinical syndrome of dyspepsia, mild diabetes, cholelithiasis, steatorrhea, and hypochlorhydria, but duodenal somatostatinoma, in general, has been clinically silent. A further contrast is that duodenal carcinoid tumors, mainly gastrinoma, tended to be benign, whereas ampullary carcinoid tumors, mainly somatostatinoma, exhibited malignant behavior. Therefore, definite diagnosis is important for treatment and prognosis, and is performed by image study, immunohistochemistry and electron microscopic examination. We report a case of somatostatinoma of the ampulla of Vater in a 51-year-old male. He complained of generalized abdominal pain for a few days. Gastrofiberscopically, a 1.2 cm sized bulging mass was observed on the ampulla of Vater. Radiologically, on abdomen CT, a protruding enhancing mass was revealed in the duodenum. In octreoscan, there was an abnormal focus off increased radiouptake in the infrahepatic area. He underwent a pancreatoduodenectomy. Grossly, the mass was an intraluminary protruding polypoid submucosal mass with focal ulceration in the ampulla of Vater. Histologically, it showed well-differentiated nonpleomorphic tubular cell nest and psammoma bodies. Immunohistochemically, the tumor cells showed a neuroendocrine nature with synaptophysin immunostain and intense staining only for somatostatin.
Abdomen ; Abdominal Pain ; Achlorhydria ; Ampulla of Vater* ; Carcinoid Tumor ; Cholelithiasis ; Diagnosis ; Duodenum ; Dyspepsia ; Gastrinoma ; Gastrointestinal Tract ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Neuroendocrine Tumors ; Pancreas ; Pancreaticoduodenectomy ; Prognosis ; Somatostatin ; Somatostatinoma* ; Steatorrhea ; Synaptophysin ; Ulcer

BACKGROUND AND OBJECTIVES: The branchial anomaly is a lateral neck mass commonly seen by otolaryngologists. Although somewhat controversial, almost all surgeons agree that lateral cervical cyst, fistula and sinus are of branchogenic origin. Depending on its anatomic location, branchial anomaly can be classified into first, second, third and fourth. The purpose of this study is to evaluate the clinical and pathologic manifestations of branchial anomaly and to determine proper diagnosis and treatment. MATERIALS AND METHOD: With a review of charts, we retrospectively analyzed 46 cases of the branchial anomaly who had been treated at the Hanyang University Medical Center between 1991 and 2001. Age, sex, site of lesion, classification, surgical therapy, radiologic and pathologic findings and post-operative surgical complications were reviewed. RESULTS: The second branchial cyst was the most common anomaly. Patients were commonly seen in the 3rd and 4th decades. Compared with cysts, the fistulas and sinuses were detected relatively at the younger age. Twenty five per cent of patients were presented in the infected state, and 45 cases were treated with complete surgical excision; there were no major post-operative complications and recurrence. CONCLUSION: An accurate diagnosis is crucial in the management of branchial anomaly to prevent recurrence and multiple surgical procedures. Although clinical examination and history are the most important factors suggesting the diagnosis of branchial anomaly, preoperative radiologic evaluation can be used to determine the nature and extent of the branchial anomaly. After control of acute inflammation or abscess, the complete surgical excision is crucial for a successful outcome.
Abscess ; Academic Medical Centers ; Branchial Region ; Branchioma ; Classification ; Diagnosis ; Fistula ; Humans ; Inflammation ; Neck ; Recurrence ; Retrospective Studies