2.Species and antimicrobial susceptibility of enteropathogenic bacteria isolated in 1986-1991.
Kwang Soo PARK ; Kye Won LEE ; Dong Il WON ; Kyung Won LEE ; Woon Seob JUNG ; Oh Hun KWON
Korean Journal of Infectious Diseases 1993;25(3):221-229
No abstract available.
Bacteria*
3.Prognostic Significance of the Tall Cell Variant of Papillary Thyroid Carcinoma: Expression of p53, bcl-2 & Leu-M1 proteins.
Won Mi LEE ; Joo Seob KEUM ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1998;32(11):1000-1007
Papillary carcinoma of the thyroid is a well differentiated neoplasm and usually has a good prognosis. However, a subset of morphologically distinct papillary carcinoma has bad prognoses. The tall cell variant of papillary carcinoma (TCPC), characterized by tall columnar cells with a height at least twice the width, is the one of these. In order to differentiate TCPC from usual papillary carcinoma (UPC) in terms of prognosis, we performed immunohistochemical studies for the expression of p53, bcl-2 and Leu-M1 proteins in 25 cases of TCPC, 26 cases of UPC and 14 cases of poorly differentiated, solid type papillary carcinoma (SPC) with an analysis of clinical parameters. The nuclear expression of p53 was noted in one case each of UPC and TCPC. The cytoplasmic p53 expression of TCPC, UPC, and SPC was observed in 17/25 cases (68%), 14/26 cases (54%), 3/14 cases (21%), respectively. bcl-2 expression was 19/25 cases (76%), 18/26 cases (69%), 5/14 cases (36%), and that of Leu-M1 was 21/25 cases (84%), 18/26 cases (69%), 4/14 cases(29%), respectively. There were no statistical significance in the expression of those immunoproteins among these three groups (p>0.05). The p53 protein was consistently expressed in the cytoplasm rather than nucleus in this study and was very well correlated to bcl-2 positivity (p<0.01). There were no statistical significance in any clinical parameters examined among these three groups (p>0.05). In conclusion, TCPC can not be separated from UPC as a distinct entity in this study and the cytoplasmic expression of p53 protein provides another mechanism of p53 inactivation in tumorigenesis of the thyroid papillary carcinoma, possibly by bcl-2 related mechanism.
Carcinogenesis
;
Carcinoma, Papillary
;
Cytoplasm
;
Immunoproteins
;
Prognosis
;
Thyroid Gland*
;
Thyroid Neoplasms*
4.Anaerobic bacteria isolated from the clinical specimens during the period of 1983 to 1992.
Ahn Na LEE ; Young Sook KANG ; Kyung Won LEE ; Yoon Seob JUNG ; Kyung Soon SONG
Korean Journal of Infectious Diseases 1993;25(1):9-17
No abstract available.
Bacteria, Anaerobic*
5.Studies on the Changes of Serum Osmolality Electrolytes, Digoxin-like Substance and Plasma Renin Activity Following Angiocardiography using Hypertonic Contrast Media.
Heon Seob SONG ; Hyeong Won SHIN ; Chan Uhung JOO ; Dae Yeol LEE ; Jin Gon JUN
Journal of the Korean Pediatric Society 1987;30(4):398-405
No abstract available.
Angiocardiography*
;
Contrast Media*
;
Electrolytes*
;
Osmolar Concentration*
;
Plasma*
;
Renin*
6.Prevalence of antibodies against chlamydia pneumoniae among blood donors and patients with tests of blood chemistry and mycoplasma pneumoniae antibody.
Yoon Seob JUNG ; Kyung Won LEE ; Hyun Sook KIM ; Oh Hyun KWON ; Sang Rae CHO
Korean Journal of Infectious Diseases 1993;25(2):131-138
No abstract available.
Antibodies*
;
Blood Donors*
;
Chemistry*
;
Chlamydia*
;
Chlamydophila pneumoniae*
;
Humans
;
Mycoplasma pneumoniae*
;
Mycoplasma*
;
Pneumonia, Mycoplasma*
;
Prevalence*
7.Clinical study on kyphoscoliosis complicating pregnancy.
Young Min KIM ; Joon Hyung KIM ; Yong Woo LEE ; Won Seob OH
Korean Journal of Obstetrics and Gynecology 1991;34(3):348-352
No abstract available.
Pregnancy*
8.Evaluation of D-Phenylalanyl-L-prolyl-L-arginine Chloromethyl Ketone(PPACK) as an Alternative Anticoagulant to Heparin Salts for Analysis of ionized Calcium, Blood Gas, Electrolytes.
Kyeong Seob SHIN ; Won Seon HAN ; Bo Ra SON ; Do Hoon LEE
Korean Journal of Clinical Pathology 1997;17(6):1002-1011
BACKGROUND: Heparin salts induce negative proportional bias according to anticoagulant concentration for analysis of ionized calcium (iCa) However, D-phenylalanyl -L-prolyl- L-arginine chloromethyl ketone (PPACK), a selective thrombin inhibitor, do not bind to ionized calcium. Therefore, we evaluated PPACK as an alternative anticoagulant to lithium heparin (Li-Hep) for analysis of ira, blood gases and electrolytes. METHODS: The concentration of iCa in whole blood anticoagulated with heparin was compared with that in serum of patients admitted to Chungbuk National University Hospital (n=27). The blood gases, electrolytes and iCa according to each anticoagulant concentration (Ll-Hep or PFACK) were analyzed. The concentrations of anticoagulated whole blood (Li-Hep; 50 kIU/L, PPACK ; 75 mumol/L) were compared with those of nonanticoagulated whole blood for blood gases, electrolytes and iCa (n=17), RESULTS: The results were as follows; whole blood anticoagulated with Li-Hep demonstrated -0.28+/-0.15 mmol/L (26.6%) bias for ira compared with serum. No bias according to each anticoagulated concentrations were observed in analysis of blood gases, potassium and chloride. Negative proportional bias for iCa and sodium in serum anticoagulated with Li-HeP was observed. In comparison, no bias for ira and sodium was observed with PPACK. No bias was observed in analysis of blood gas or electrolytes with each anticoagulated whole blood except for sodium and chloride that had clinically nonsignificant bias. Whole blood anticoagulated with Li-Hep demonstrated a consistent -0.08+/-0.02 mmol/L (6.3%) bias for ira compared with nonanticoagulated whole blood. In comparison, no bias was observed with PPACK-anticoagulated whole blood for iCa. CONCLUSIONS: We concluded that PPACK is an ideal anticoagulant without bias for analysis of iCa, blood gases and electrolytes.
Arginine
;
Bias (Epidemiology)
;
Calcium*
;
Chungcheongbuk-do
;
Electrolytes*
;
Gases
;
Heparin*
;
Humans
;
Lithium
;
Potassium
;
Salts*
;
Sodium
;
Thrombin
9.Dopaminergic Neurons in the Olfactory Bulb: A Differences in the Insectivore and Rodents.
Young Gil JEONG ; Nam Seob LEE ; Moo Ho WON
Korean Journal of Anatomy 1999;32(3):361-374
These studies document species differences in the distribution of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) within the main olfactory bulb (MOB) of a number of rodents and insectivore species including the rat, wild mouse, mongolian gerbil, stripped field mouse (apodemus agrarius), hedgehog, mole, laboratory shrew (suncus murinus). TH-containing neuronal perikarya were observed in the MOB of the both species of the rodents and insectivore except the hedgehog and laboratory shrew (suncus murinus). None of these cell groups displayed either dopamine beta hydroxylase (DBH) or phenylethanolamine-N-methyltransferase (PNMT). The number of stained somata and their intensity varied such that label was most prominent in the stripped field mouse followed in decreasing order by the rat, mongolian gerbil, wild mouse and mole. The vast majority of such cells occurred in the glomerular layer as periglomerular cells surrounging the glomeruli of the stripped field mouse, rat, mongolian gerbil, wild mouse and moles. Numerous additional cells were present in the external plexiform layer (EPL) and mitral cell layer (MCL). These often displayed long ascending immunoreactive processes and appeared to correspond to tufted cells. Also a few smaller, multipolar cells were present in the internal granular layer scattered among the granule cells. However, the hedgehog and laboratory shrew displayed no perikaryal staining in the MOB. In conclusion, these data suggest that TH is present in the MOB of stripped field mouse, rat, mongolian gerbil, wild mouse and moles but is not found in the MOB of the hedgehog and laboratory shrew, or that species differences exist in the level of TH.
Animals
;
Dopamine beta-Hydroxylase
;
Dopaminergic Neurons*
;
Gerbillinae
;
Hedgehogs
;
Mice
;
Neurons
;
Olfactory Bulb*
;
Rats
;
Rodentia*
;
Shrews
;
Tyrosine 3-Monooxygenase