Ia antigen (HLA-DR in rnan) is confined to the Langerhans cells and indeterminate cells in the normal epidermis in man. However the keratinocyte express la antigen in a variety of dermatoses. IFN-r (irnmune interfcron), known as macrophage activating factor, has been shown to induce Ia antigen expression in a wide variety of cell types. However the Ia antigen induced by 1FN-r is inhibited PGE2, a product of cyclooxygenase pathway of arachidonic acid metabolism. LTB4, a product of lipoxygenase pathway, can replace the IL-2 requirement for IFN-r production in the lymphocytes. There are three main morphalogical patterns of Ia antigen staining in the epidennis. Staining of the basal cells, staining in the mid epidermis in Malphigian layer and staining throughout the epidermis. The staining of Ia' keratinocyte was found to be confined to the lesional area of contact hypersensitivity reaction, graft vs host disease, and lichen planus. la+staining to extend beyond the lesional area was also seen in the study on turberculosis and leporsy.
Arachidonic Acid ; Dermatitis, Contact ; Dinoprostone ; Epidermis ; Graft vs Host Disease ; Histocompatibility Antigens Class II ; Interleukin-2 ; Keratinocytes* ; Langerhans Cells ; Leukotriene B4 ; Lichen Planus ; Lipoxygenase ; Lymphocytes ; Macrophages ; Metabolism ; Prostaglandin-Endoperoxide Synthases ; Skin Diseases

No abstract available.
Animals ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea* ; Spleen*

CsA per os during the early sensitization period caused potentiation of 14-day shin response and this response was enhanced in group D, CsA given on days 3 7 Thereafter, suppression of CHS began in group E, CsA was given on days 6- 1G, and this tendency continue to the time of full sensitization of guinea pigs (group G). 27701173 We report herein a case of alopecia mucinosa in a 46-year-old male. He had a coin-sized erythematous hairless plaque on the parietal area. Histopathologic examinations, including much stains, showed typical findings of alopecia mucinosa. Thc present case, appeared on the scalp, might be an acute benign type of the disease.
Animals ; Coloring Agents ; Cyclosporine* ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Dronabinol ; Guinea Pigs* ; Guinea* ; Humans ; Male ; Middle Aged ; Mucinosis, Follicular ; Rabeprazole ; Scalp

Porphyria is a rare metabolic disorder in this country and a few cases of acute intermittent porphyria has been reported. We observed a case of porphyria cutanea tarda associated with liver cirrhosis. The patient was 61-year-old farmer with heavy alcoholic habit. He had been suffered from skin fragility and photosensitivity for 3 years. His face color was slate blue and sclerdermoid appearance noted especially on the cheek. Bullae, which is healing slowly and followed by atrophic pigmented scars, were developed on the dorsum of hand and feet after receiving trivial trauma and massive alcohol intake. None of his family members has similar symptoms. Urine specimen showed port-wine coIor and fluoresced pinkish under the Wood's light. Serum iron level was markedly elevated (400 microgram%). The other abnormal findings of liver function test were BSP retention (16%/45min.), elevated SGOT (198 unit) and SGPT (80 unit) levels. Esophagram revealed suspicious of varices and liver scanning showed cirrhotic changes. Skin biopsy specimens taken from the cheek and dorsum of hand showed sclermoid changes and subepidermal bulla. Liver biopsy disclosed mild degree of cirrhotic changes.
Alanine Transaminase ; Alcoholics ; Aspartate Aminotransferases ; Biopsy ; Cheek ; Cicatrix ; Foot ; Hand ; Humans ; Iron ; Liver ; Liver Cirrhosis ; Liver Function Tests ; Middle Aged ; Porphyria Cutanea Tarda* ; Porphyria, Acute Intermittent ; Porphyrias* ; Skin ; Varicose Veins

There have been published manuscripts which fully suggest that there may be a topographic variance of contact sensitivity in experimental animals such as guinea pigs, hamsters and mice, probably due to a large extent to the topographic difference of Langerhans cell population and to a little extent to impact on the induction site. This assay was done to establish the possibility of involvement of a certain topographic variance in contact sensitivity reaction. Three different anatomical sites were chosen for the induction in three groups of five guinea pigs: the conventional site of the nuchal region, and the sacral aod. ahdominal skin regions. For the elicitation, two different concentrations of DNCB were applied on three sites along both sides of the spine in the back. The contact sensitivity reaction elicited by DNCB in the group of the nuchal abdominal skin induction was definitely stronger at the site closer to the nuchal region than that found at sites closer to the sacral regior. but all animals of the group sensitized on the sacral region showed comparatively uniform reaction. Zvidently, contact sensitivity reaction is influenced mainly by topographic variance hut shows a litte difference depending upon induction sites.
Animals ; Cricetinae ; Dermatitis, Contact* ; Dinitrochlorobenzene ; Guinea Pigs* ; Guinea* ; Mice ; Sacrococcygeal Region ; Skin ; Spine

The current experiment pursued the time course of UVB-induced immune suppression of DNCB contact hypersensitivity(CHS) in guinea pigs. Both group B and C, received suberythemal doses of UVB on days -3, -2, -1, 0 and -6, -5, -4, 3 respectively, showed marked suppressions of CHS (56.36p;and56.25p,). However, the recovery of CHS from the UVB-induced suppression started by group D(UVB on days -9, -8, -7, -6: 67. 36%) and further extended to subsequent group E(UVB on days -12, -11, -10, -9: 78.82%), Group F(UVB on days -15, -14, -13, -12: 79.86%) and the final group G(UVB on days -18, -17, -16, -15: 81. 94%) in which the recovery of CHS slightly exceeded the control (group A:79. 86%).
Animals ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea*

With recent progres ion in knowledge of immunology, the roles of eosinophils and basophils in immediate and delaye3 hypersnsitivity reatioons have been partly elucidated and generally they are known as marker cells in various allergic disorders. Changes in number of eosinophils and basophils in the infiltrates of the involved tissue or in circulating blood. in various allergic disordcrs have been reported by several investigators, however, there are only a fever ports about skin disorders, especially for basophils because staining and conting method of the basophils devcloped only recently by James and Moore. The present study has been performed to detect the alteration in absolute number of circulating eosinophils and basophils and to find out any correation between the changes of hoth cell numbers in various allergic dermatoses. A total of 84 pati,.nts with various allergic dermatoses and 50 healthy adults as control group were selected for this study at National Medical Center during the period of July to September 1976. The method of staining and counting of circulating eosinophils and basophils was essentially same with the originaI method of Randolph for eosinophils and James and Moore for basophils with only a minor modifications of both. All the blood specimens were taken between 9:00 and l2:00 a.m. and all the experiments were done within 6 hours after sampling. The results were as follows. 1. The mean value+1 S.D. for eosinophil, basophil and total leukocyte number in 50 normal control was 126.5+80.4, 31. 413.6, and 5,928.3+1,536.9, respectively. 2. In 20 cases of acute urticaria (duration of illness, less than 2 days), the mean value+1 S.D. for eosinophil, basophil, and total leukocyte number was 107.4+95.4, 9.3+7.3 and 9,620+3,240.5, respectively and the result showed a significant decrease of basophils(p<0.001). In 20 cases af chronic urticaria (duration of illness, more than 4 weeks and dermographism included), the mean value 1 S.D. for eosinophil, basophil and total leukocyte number was 237.3+202.9, 31.1+12.3 and 7,748.5+1,989,7, respect!vely and the result showed a significant increase of eosinophils(p<0.01). 4. In 15 cases of atopic dermatitis, the mean value+1 S.D. for eosinophil, basophil and total leukocyte number was 309.7+402.8, 28.8+16.5 and 7,694.5+3,221.9, respectively and the result showed a significant increase of eosinophils (p<0.01). 5. !n 10 cases of acute allergic contact dermatitis, the mean value+1 S.D. for eosinophil, basophil and total leukocyte number was 381.5+269.5, 53.6+34.0 and 7,979.4+1,126.0 respectively and the result showed a significant increase of eosinophils and basophils, respectively(p<0.001, p<0.0.1) 6. In 5 ceases of allergic drug eruption, the mcan value 1 S. D. for eosinophil, basophil and total leukocyte number was 520.0+367.5, 19.6+17.6 and 12,390+2,783.3, respectively and the result showed a significant in.rease of eosinophils (p<0. 001) and decrease of basophils. 7. In 10 cases of scabies, the mean value+1 S.D. for eosinophil, basophil and totai leukocyte number was 299.3+216.6, 30.4+11.5 and 8,081.6+3,304.4, respectively and the result showed a significant increase of eosinophils (p<0.01). 8. In 3 cases of tinea pedis with id eruption, the mean value+1 S.D. for eosinophil, basophil and total leukocyte number was 288.9+79.7, 29.6+5.2 and 8,916.7+2,739.2, respectively and the result shovred slight increase of eosinophils. 9. In order to observe the sequential changes in the number of circulating eosinophils and basophils, a patient with wart was sensitized and challenged with DNCB, and serial determinations were performed. During sensitization period the number of circulating eosinophils was not changed, but the basophils increased gradually, After spontaneous flare-up the number of eosinophils increased with the decrease toward normal of basophils. In the present study, we confirmed that the circulating eosinophils and basophils are definitely related to allergic dermatoses and found out the possibility that the difference in absolute number might be related to the causal agents and duration of disease.
Adult ; Allergy and Immunology ; Basophils* ; Cell Count ; Dermatitis, Allergic Contact ; Dermatitis, Atopic ; Dinitrochlorobenzene ; Drug Eruptions ; Eosinophils* ; Fever ; Humans ; Leukocyte Count ; Research Personnel ; Scabies ; Skin ; Skin Diseases* ; Tinea Pedis ; Urticaria ; Warts

The mite fauna of the house-dust, collected from 39 houses of Seoul, were examined and the results are as follows. 1) Mites were present in 29 samples (74.3%) and the number of mites collected was 993 in total. 2) The families of the mites distinguished were Pyroglyphidae, Acaridae, Cheyletidae, Phytoseiidae and Smariidae, and the identified species were Dermatophagoides farinae, Deromatophagoides pteroassinus, Tyrophagus purtrecentiae, Landoglyphus konoi and Cheyletus malacensis. 3) D. farinae (53.6%) showed higher frequency rate of occurance than any other mites; C. malacensis (12.8%), T. putrecentiae (7.7%), D. pteronyssinus (5.0%) and I. konoi (2.7%).
Acaridae ; Dermatophagoides farinae ; Dust* ; Humans ; Mites* ; Pyroglyphidae ; Seoul

A case of solitary glomuys tumor affection 43 year-old woman, who has been suffered from bluish purple colored, smail, tener nodule on the left scapular area, was presented. Surgical excision of lesion was enough for relieving the whole subjective symptoms.
Adult ; Female ; Glomus Tumor* ; Humans

The pattern of DNCB (2, 4-dinitrochlorobenzene) oral desensitization and its antigenic specificity were investigated in guinea pigs. In search of antigenic specificity of DNCB oral desensitization, animals were fed oxazolone (4-ethoxymethylene-2- phenyl-oxazol-5-one) in a DNCB presensitized group, and conversely, DNCB fed in an oxazolone presensitized group. For the study of the pattern of oral desensitization, guinea pigs were initially sensitized to DNCB and followed by feeding of DNCB for 6 days, and challenged on the 13th, 21st, 29th, 37th and 45th days after sensitization. Oxazolone feeding in DNCB presensitized guinea pigs had no effect on the development of the fully responsive DNCB contact sensitivity (72. 85g), and DNCB feeding in oxazolone pre-primed animal had no effect on the development of oxazolone contact sensitivity (79. 37g). On the contrary, oxazolone feeding in DNCB preprimed guinea pigs and DNCB feeding in oxazolone pre-primed resulted in, respectively, oxazolone and DNCB oral tolerance (44.44% p < 0.01 & 42.06%p<0.01). The effect of desensitization appeared even one day before the completion of 6 days' feeding and the efficacy lasted about 30 days by the natural waning of contact sensitivity.
Animals ; Dermatitis, Contact ; Dinitrochlorobenzene* ; Epitopes ; Guinea Pigs* ; Guinea* ; Oxazolone