PURPOSE: The purpose of this study was to investigate factors associated with suspicious developmental delay in infants and early childhood. METHODS: Participants were 133 infants, aged from birth to 6 years old and their mothers, who were being seen at 16 Public health centers in B city. Korean Denver II was used to test infant development. chi2-test, Fisher's exact test and multiple logistic regression were used with SPSS 19.0 to analyze data. RESULTS: Of participant infants, 7.5% were below the 3rd percentile for the weight percentile, 8.4% is a weight curve that crosses more than 2 percentile lines on the growth charts after previous achievement, and 9.8% had suspicious developmental delay according to Korean Denver II. Further the predictive factors related to suspicious development delay in the children were decrease of weight percentile (Odds Ratio [OR]=6.69, Confidence Interval [CI])=1.22-36.45), low economic state (OR=6.26, CI=1.50-26.00), and developmental delay perceived by their mothers (OR=4.99, CI=1.24-20.06). CONCLUSION: It is necessary to build a government level system to follow management of development of infants and children from the time of birth. Especially, it is necessary to develop a program for children in low income families.
Achievement ; Aged ; Child ; Child Development ; Growth Charts ; Humans ; Infant ; Logistic Models ; Mothers ; Parturition ; Public Health

No abstract available.
Angiofibroma*

Trichoblastoma is a rare benign neoplasm that arises in the follicular germinative cells. The terminology and histopathological definition of this tumor is both complex and confusing. Trichoblastoma occurs only on the hair bearing areas such as the scalp, face and pelvic area with varying degrees in size. We describe a case of trichoblastoma occurring as a nodule on the nose. The tumor was composed of numerous lobules of basaloid epithelial cell nests with distinct peripheral palisading of nucleoli that were clearly demarcated from the stroma.
Epithelial Cells ; Hair ; Nose ; Scalp

Indirect composite resin are used as an popular effective esthetic material in prosthetic dentistry, often with metallic substructure that provides support for restorations. Recently, new indirect composite resins as a substitute of ceramic have been developed. These resins provide good esthetics, with a wide range of hue and chroma. And the flexural strength of those is in the range of 120-150MPa, which is higher than that of feldspathic ceramic, and similar tot hat of Dicor. Although it has many merits, one of the major clinical problems of composite resins is the bond failure between metal and resin due to insufficient interfacial bond strength. The purpose of this study was to evaluate shear bond strength of the reinforced indirect composite resin to dental alloys. Three different composite resin systems(Artglass . Sculpture , Targis ) as test groups and ceramic(VMK 68 ) as control group were bonded to Ni-Cr-Be alloy(Rexillium III ) and gold alloy(Dave 4). All specimens were stored at 37degrees C distilled water for 24 hours and the half of specimens were thermocycled 2000 times at temperature from 5degrees C to 60degrees C. The shear bond strengths of reinforced indirect composite resins to dental alloys were measured by using the universal testing machine, and modes of debonding were observed by stereoscope and scanning electron microscope. The results were as follows : 1. The shear bond strengths of reinforced indirect composit resins to dental alloys were approximately half those of ceramic to dental alloys(P<0.01). 2. There was no significant difference between the shear bond strength of several reinforced indirect composite resins to metal. 3. Alloy type did not affect on the shear bond strengths of resin to metal, but the shear bond strengths of ceramic to gold alloys were higher than those of ceramic to Ni-Cr alloys(P<0.05). 4. The shear bond strenghts of Artglass and Targis to gold alloys were significantly decreased after thermocycling treatment(P<0.01). 5. Sculpture showed cohesive, adhesive, and mixed failure modes, but Artglass and Targis showed adhesive or mixed failures. And ceramic showed cohesive and mixed failures.
Adhesives ; Alloys ; Ceramics ; Composite Resins* ; Dental Alloys* ; Dentistry ; Esthetics ; Gold Alloys ; Sculpture ; Water

Phacomatosis pigmentovascularis (PPV) is a rare syndrome characterized by concurrent nevus flammeus (capillary malformation) and pigmentary nevus. According to current research, the major pathophysiologic mechanism in PPV is venous dysplasia with resultant compensatory collateral channels and venous hypertension. Arterial involvement is rare. We herein report our experience on renovascular hypertension, intermittent claudication, and severe rhabdomyolysis due to diffuse stenosis of multiple arteries in a patient with PPV type IIb associated with SWS.
Arteries ; Constriction, Pathologic ; Humans ; Hypertension ; Hypertension, Renovascular ; Intermittent Claudication ; Intracranial Aneurysm ; Neurocutaneous Syndromes* ; Nevus ; Port-Wine Stain ; Rhabdomyolysis* ; Sturge-Weber Syndrome* ; Vascular Diseases

PURPOSE: Troglitazone (TRO) is a peroxisome proliferator-activated receptor gamma (PPARgamma) agonist. TRO has antiproliferative activity on many kinds of cancer cells via G1 arrest. TRO also increases Cu2+/Zn2+-superoxide dismutase (CuZnSOD) and catalase. Cell cycle, and SOD and catalase may affect on radiation sensitivity. We investigated the effect of TRO on radiation sensitivity in cancer cells in vitro. MATERIALS AND METHODS: Three human cervix cancer cell lines (HeLa, Me180, and SiHa) were used. The protein expressions of SOD and catalase, and catalase activities were measured at 2-10 microM of TRO for 24 hours. Cell cycle was evaluated with flow cytometry. Reactive oxygen species (ROS) was measured using 2',7'-dichlorofluorescin diacetate. Cell survival by radiation was measured with clonogenic assay. RESULTS: By 5 microM TRO for 24 hours, the mRNA, protein expression and activity of catalase were increased in all three cell lines. G0-G1 phase cells were increased in HeLa and Me180 by 5 microM TRO for 24 hours, but those were not increased in SiHa. By pretreatment with 5 microM TRO radiation sensitivity was increased in HeLa and Me180, but it was decreased in SiHa. In Me180, with 2 microM TRO which increased catalase but not increased G0-G1 cells, radiosensitization was not observed. ROS produced by radiation was decreased with TRO. CONCLUSION: TRO increases radiation sensitivity through G0-G1 arrest or decreases radiation sensitivity through catalase-mediated ROS scavenging according to TRO dose or cell types. The change of radiation sensitivity by combined with TRO is not dependent on the PPARgamma expression level.
Catalase ; Cell Cycle ; Cell Line ; Cell Survival ; Cervix Uteri ; Chromans ; Female ; Flow Cytometry ; Fluoresceins ; Humans ; PPAR gamma ; Radiation Tolerance ; Reactive Oxygen Species ; RNA, Messenger ; Thiazolidinediones ; Uterine Cervical Neoplasms

The propose of this study was to quantify the changes of soft tissue profile following orthodontic treatment and to evaluate the relationship of those to the skeletal elements. Pre-and post-treatment lateral cephalometric head films of 40 cases(20 extraction cases, 20 non-extraction camas) were traced, and the changes following treatment were measured and quantified by digital subtraction method, and statisticall analyzed. The obtained results were as follows; 1. in extraction group, the change of upper lip area(UL) was 558.60+/-355.17 pixels, that of lower lip area(LL) was 941.15+/-364.07 pixels. But, in non-extraction group the change of uper lip area(UL) was 125.65+/-404.16 pixels, that of lower lip area(LL) was 104.05+/-440.93 pixels, which was significantly lesser than those in extraction group. 2. In extraction group, there was significant correlationship between upper lip area change(UL) and difference of upper incisor point(deltaUIP). Lower lip area change(LL) was significantly correlated with difference of upper incisor(deltaUIP), difference of Frankfort upper incisor angle(deltaFUIA) or difference of interincisal angle(deltaIIA). 3. In extraction group, the ratio of difference of upper incisor point(deltaUIP) to difference of labrale superius(deltaLS) was 1.68; difference of lower incisor point(deltaLIP) to difference of labrale inferius(deltaLI) was 1.19; difference of upper incisor point(deltaUlP) to increment in upper lip thickness(deltaTUL) was 1.95. 4. In non-extraction group, there was a significant cortelationship between upper lip area change(UL) and difference of upper incisor point(deltaUIP).
Head ; Incisor ; Lip

Many of early findings regarding intestinal stem cells (ISCs) and their niche in the human intestine have relied on colorectal cancer cell lines and labor-intensive and time-consuming mouse models. However, these models cannot accurately recapitulate the physiologically relevant aspects of human ISCs. In this study, we demonstrate a reliable and robust culture method for 3D expanding intestinal spheroids (InSexp ) mainly comprising ISCs and progenitors, which can be derived from 3D human intestinal organoids (HIOs). We did functional chararcterization of InSexp derived from 3D HIOs, differentiated from human pluripotent stem cells, and optimization culture methods. Our results indicate that InSexp can be rapidly expanded and easily passaged, and show enhanced growth rates via WNT pathway activation. InSexp are capable of exponential cell expansion and cryopreservation. Furthermore, in vitro-matured HIO-derived InSexp proliferate faster than immature HIO-derived InSexp with preservation of the parental HIO characteristics. These findings may facilitate the development of scalable culture systems for the long-term maintenance of human ISCs and provide an alternative platform for studying ISC biology.

PURPOSE: The fibrates are ligands for peroxisome proliferator-activated receptor (PPAR) alpha and used clinically as hypolipidemic drugs. The fibrates are known to cause peroxisome proliferation, enhance superoxide dismutase (SOD) expression and catalase activity. The antioxidant actions of the fibrates may modify radiation sensitivity. Here, we investigated the change of the radiation sensitivity in two cervix cancer cell lines in combination with fenofibrate (FF). MATERIALS AND METHODS: Activity and protein expression of SOD were measured according to the concentration of FF. The mRNA expressions were measured by using real time reverse-transcription polymerase chain reaction. Combined cytotoxic effect of FF and radiation was measured by using clonogenic assay. RESULTS: In HeLa cells total SOD activity was increased with increasing FF doses up to 30 microM. In the other hand, the catalase activity was increased a little. As with activity the protein expression of SOD1 and SOD2 was increased with increasing doses of FF. The mRNAs of SOD1, SOD2, PPARalpha and PPARgamma were increased with increasing doses of FF. The reactive oxygen species (ROS) produced by radiation was decreased by preincubation with FF. The surviving fractions (SF) by combining FF and radiation was higher than those of radiation alone. In Me180 cells SOD and catalase activity were not increased with FF. Also, the mRNAs of SOD1, SOD2, and PPARalpha were not increased with FF. However, the mRNA of PPARgamma was increased with FF. CONCLUSION: FF can reduce radiation sensitivity by ROS scavenging via SOD induction in HeLa. SOD induction by FF is related with PPARalpha.
Catalase ; Cell Line ; Fenofibrate ; Fibric Acids ; Hand ; HeLa Cells ; Humans ; Hypolipidemic Agents ; Ligands ; Peroxisomes ; Polymerase Chain Reaction ; PPAR alpha ; PPAR gamma ; Radiation Tolerance ; Reactive Oxygen Species ; RNA, Messenger ; Superoxide Dismutase ; Superoxides ; Uterine Cervical Neoplasms

PURPOSE: E-cadherin(E-CD) is a family of Ca++-dependent intercellular adhesion molecules, plays essential roles in organogenesis and in the maintenance of normal structure and function. Decreased expression of E-CD correlates with tumor aggressiveness, advanced stage and metastasis. In renal cell carcinoma(RCC), however, this correlation is not well established and prevalence of negative expression of E-CD is higher than in other carcinomas. We focused our interest on E-CD expression in RCC and its clinicopathological implications. MATERIALS AND METHODS: Retrospectively, we reviewed 34 cases with RCC that were reclassified according to Mainz classification. Tumor stage, grade were determined according to the Robson method and Fuhrmann`s grading system. We detected E-CD expression in RCC by immunohistochemical staining and investigated the relationship between E-CD expression and clinicopathological features including prognosis. RESULTS: Thirty four cases of RCC were consisted of 21 cases(61%) of non-papillary clear cell type, 2 cases(6%) of chromophil type, 3 cases(9%) of spindle cell type and 8 cases(24%) of chromophobe type by Mainz classification. Especially, E-CD expression showed a strong positive reaction along the cell membranes in all 8 cases of chromophobe type. Our immunohistochemical study of E-CD expression in 34 RCC specimens showed 73% of positive expression and 27% of negative expression. Absence of E-CD expression was correlated with the high tumor stage and grade( stage & grade 3 or 4. p<0.05 in chi-squar test). All of metastatic cases showed a negative expression of E-CD. CONCLUSIONS: The differential expression of E-cadherin in renal cell carcinomas suggest that the tumor cell origin and differentiation. Especially, chromophobe cell type may have different biologic characteristics from other types of renal cell carcinoma. In this study, E-CD expression was seemed to be lost in RCC with a high stage and grade. In addition, preserved E-CD expression was associated with better prognosis than reduced E-CD expression.
Cadherins* ; Carcinoma, Renal Cell* ; Cell Adhesion Molecules ; Cell Membrane ; Classification ; Humans ; Neoplasm Metastasis ; Organogenesis ; Population Characteristics ; Prevalence ; Prognosis ; Retrospective Studies