Inflammation plays an important role in host defense against external stimuli such as infection by pathogen, endotoxin or chemical exposure by the production of the inflammatory mediators that produced by macrophage. Anti-inflammatory factor is important to treat the dangers of chronic inflammation associated with chronic disease. This research aims to analyze the anti-inflammatory effects of Garcinia mangostana L. peel extract (GMPE), α-mangostin, and γ-mangostin in LPS-induced murine macrophage cell line (RAW 264.7) by inhibiting the production of inflammatory mediators. The cytotoxic assay of G. mangostana L. extract, α-mangostin, and γ-mangostin were performed by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to determine the safe and non-toxic concentration in RAW 264.7 for the further assay. The concentration of inflammatory mediators (COX-2, IL-6, and IL-1β) were measured by the ELISA-based assay and NO by the nitrate/nitrite colorimetric assay in treated LPS-induced RAW 264.7 cells. The inhibitory activity was determined by the reducing concentration of inflammatory mediators in treated LPS-induced RAW 264.7 over the untreated cells. This research revealed that GMPE, α-mangostin, and γ-mangostin possess the anti-inflammatory effect by reducing COX-2, IL-6, IL-1β, and NO production in LPS-induces RAW 264.7 cells.
Cell Line ; Chronic Disease ; Fibrinogen ; Garcinia mangostana* ; Inflammation ; Interleukin-6 ; Macrophages ; RAW 264.7 Cells

Objective:To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7).Methods:A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells.Interleukins (IL-6,IL-1β),tumor necrosis factor-tα (TNF-α),and cyclooxigenase-2 (COX-2) levels were measured by ELISA,and nitric oxide (NO) levels measured by a nitrate/nitrite colorirnetric assay to determine the inhibition activity of OTEE and EGCG.Results:Lipopolysaccharide induction increases NO,COX-2,IL-6,IL-1β,and TNF-α levels compared with the untreated cell (negative control).The positive control,lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study.The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO,COX-2,IL-6,IL-1 β,and TNF-α.OTEE had a higher inhibition activity toward NO,COX-2,IL-6,and IL-1 β than EGCG;the reverse was seen for TNF-α.However,both OTEE and EGCG suppressed production of NO,COX-2,IL-6,IL-1β,and TNF-α.Conclusions:OTEE and EGCG have the potential for use as anti-inflammatory drugs,which is shown by their ability to reduce the production of NO,COX-2,IL-6,IL-1β,and TNF-α in active macrophages.

Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 µmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
Apoptosis ; Atherosclerosis ; Catechin ; Electrocardiography ; Endothelial Cells ; Endothelial Progenitor Cells* ; Flavonoids* ; Hydrogen ; Oxidative Stress ; Reactive Oxygen Species* ; Tea* ; Vascular Endothelial Growth Factor Receptor-2

Dyslipidemia is a condition of fat metabolism disorders with increased levels of total cholesterol (CHOL), triglycerides (TG), low-density lipoprotein (LDL), and low high-density lipoprotein (HDL). Dyslipidemia is often found in patients with type 2 diabetes mellitus (DMT2) with a higher risk of cardiovascular disease.The extract of butterfly pea (Clitoria ternatea L.) (CTE), exhibits therapeutic action like antioxidant, antiinflammatory and antidiabetic properties. This study examined the antidiabetic potential of CTE in rat models of dyslipidemia and DM. Rats were fed nicotinamide (NA) and streptozotocin (STZ) to induce DMT2 after a 28-day high-fat diet, and the rats were given with CTE at 200, 400, 800 mg/kg body weight (BW), glibenclamide, and simvastatin for 28 days. Suppressor of mothers against decapentaplegic homolog 3 (SMAD3), and SMAD4 were assayed by immunohistochemistry (IHC), regenerating family member 1 alfa (REG1A), REG1B and glucagon gene expression were measured by quantitative reverse transcription polymerase chain reaction (RTqPCR) body weight was measured weekly. CTE at a dose of 800 mg/kg BW was the most active to increase body weight, and decrease SMAD3, SMAD4 protein expression, glucagon, REG1A, REG1B genes expression in dyslipidemia and DM rat model. CTE has potency antidiabetic activities in dyslipidemia and DM rat mode

Skin aging is a complex biological process due to intrinsic and extrinsic factors. Free radical oxidative is one of extrinsic factors that induce activation of collagenase, elastase and hyaluronidase. Natural product from plants has been used as antioxidant and antiaging. This study aimed to evaluate antioxidant and antiaging properties of Hibiscus sabdariffa extract (HSE) and its compounds including myricetin, ascorbic acid, and β carotene. The phytochemical of H. sabdariffa was determined using modified Farnsworth method and presence of phenols, flavonoids and tannins were in moderate content, whereas triterpenoids and alkaloids were in low content. Total phenolic content performed using Folin-Ciocalteu method, was 23.85 µg GAE/mg. Quantitative analysis of myricetin, β-carotene, and ascorbic acid of HSE was performed with Ultra-High Performance Liquid Chromatography (UHPLC) that shows 78.23 µg/mg myricetin, 0.034 µg/mg β-carotene, whilst ascorbic acid was not detected. HSE has lower activity on DPPH (IC₅₀ = 195.73 µg/mL) compared to β-carotene, the lowest in ABTS assay (IC50 = 74.58 µg/mL) and low activity in FRAP assay (46.24 µM Fe(II)/µg) compared to myricetin, β-carotene. Antiaging was measured through inhibitory activity of collagenase, elastase, and hyaluronidase. HSE had weakest collagenase inhibitory activity (IC₅₀= 750.33 µg/mL), elastase inhibitory activity (103.83 µg/mL), hyaluronidase inhibitory activity (IC₅₀ = 619.43 µg/mL) compared to myricetin, β-carotene, and ascorbic acid. HSE contain higher myricetin compared to β-carotene. HSE has moderate antioxidants and lowest antiaging activities. Myricetin is the most active both antioxidant and antiaging activities.
Alkaloids ; Antioxidants ; Ascorbic Acid ; Biological Processes ; Carotenoids ; Chromatography, Liquid ; Collagenases ; Flavonoids ; Hibiscus* ; Hyaluronoglucosaminidase ; Methods ; Pancreatic Elastase ; Phenol ; Phenols ; Skin Aging ; Tannins

Objective To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), and cyclooxigenase-2 (COX-2) levels were measured by ELISA, and nitric oxide (NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG. Results Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1β, and TNF-α levels compared with the untreated cell (negative control). The positive control, lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2, IL-6, IL-1β, and TNF-α. OTEE had a higher inhibition activity toward NO, COX-2, IL-6, and IL-1β than EGCG; the reverse was seen for TNF-α. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1β, and TNF-α. Conclusions OTEE and EGCG have the potential for use as anti-inflammatory drugs, which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1β, and TNF-α in active macrophages.

Objective: To evaluate the potential effect of human Wharton's jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×10 4 /mL, 5×10 5 /mL, 5×10 6 /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL-18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.