Objective To investigate the effect of interventional treatment in coronary heart disease (CHD) in elderly patients with brain natriuretic peptide and high-sensitivity C-reactive protein (hsCRP) levels.Methods 130 elderly patients undergoing coronary angiography interventional treatment were selected for the observation group,30 cases with normal coronary arteries angiography were chosen as the control group.In 46 cases of acute myocardial infarction(AMI) group,34 cases of unstable angina pectoris(UAP) group and 50 patients with stable angina pectoris (SAP) group and 30 cases coronary angiography normal control group(NOR),the hs-CRP and plasma brain natriuretic peptide(BNP) differences were determined and compared.Results CHD (AMI group,UAP group and SAP group) in patients with BNP levels were (548.6 ± 200.4) mg/L,(521.0 ± 198.7) mg/L and (175.8 ± 119.4) mg/L,which were significantly higher (59.3 ± 45.7) mg/L in the control group,hsCRP levels only in patients with acute myocardial infarction and unstable angina was significantly increased,the difference was statistically significant (t =13.74,10.65,all P ＜ 0.05).3d after PCI and three months prior CHD hsCRP increased to (45.8 ± 15.3)ng/L,and then decreased to (8.6 ± 4.4) ng/L,significantly different (t =10.76,14.54,all P ＜ 0.05) ; while BNP levels have been falling,first down (247.4 ± 70.1)mg/L,3 months after the falls (69.8 ± 20.0)mg/L,the difference was significant(t =11.47,19.64,all P＜0.05).Conclusion The levels of brain natriuretic peptide,hsCRP are reflections of coronary stenting in patients with coronary heart disease early postoperative inflammatory reaction and evaluate a sensitive indicator of disease,which may play an role in stent restenosis occurs.

Objective To observe the efficacy of Er ∶ YAG laser and pulsed dye laser (PDL) in treating facial acne scars.Methods A total of 78 cases with facial acne scars were divided into two groups.The test group included 40 patients that were treated by Er ∶ YAG laser (total three treatments,once three months)and irradiated by PDL (total four times,once per month),while the control group included 38 patients treated by Er ∶ YAG laser only.After three months of the last treatment,the effective rate and side effects were recorded and compared.Results The efficacy of the test group reached 92.5％ (37/40) and 77.5％ (31/40) for acne scars and post-acne erythema,respectively.However,the effective rate of the control group was 73.7％ (28/38) and 47.4％ (18/38).There were statistically significant differences between two groups (P＜0.05).Regarding reported adverse effects,there were four cases in the test group and two cases in the control group that appeared mild pigmentation.After symptomatic treatment,the skin color recovered.There was no other side effect in both two groups.Conclusions The effectiveness of Er ∶ YAG laser and PDL is better than Er ∶ YAG laser only in treating facial acne scars,and thus it is worthwhile for popularization and application in acne scars.

Objective To explore the therapeutic effect, mechanism and safety of Qingrehuashi recipe in treating chronic superficial gastritis with erosion pertaining to spleen-stomach damp-heat syndrome. Methods 84 cases of chronic superficial gastritis with erosion were recruited into a control group and a treatment group randomly, with each groups containing 42 cases. The treatment group was treated with Qingrehuashi recipe, while the control group was treated with Qingweizhitong granule. Both groups were treated for 8 weeks. The clinical manifestations and signs, gastroscopic results and pathological effects were observed before and after the treatment. Results The total effective rate and curative rate in the treatment group were superior to those in the control group (P<0.05) . Conclusion Qingre Huashi recipe is effective and safe in in treating chronic superficial gastritis with erosion pertaining to spleen-stomach damp-heat syndrome. The mechanism might be related to its effects of anti-inflammation, anti-oxidation, eradicating Hp, promoting gastric motility and enhancing gastric mucosal barrier.

Objective To investigate the the efficacy and safety of recombinant human brain natriuretic peptide (rhBNP) in the treatment of acute non-ST-segment elevation myocardial infarction patients with heart failure.Methods 80 patients with heart failure caused by acute non-ST-segment elevation myocardial infarction were chosen,they were divided into the observation group and control group based on a random number table,each group included 40 patients.The observation group were treated with intravenous injection of recombinant human brain natriuretic peptide,the control group were treated with intravenous nitroglycerin.vital signs,dyspnea,brain natriuretic peptide (BNP),associated hemodynamic changes and adverse reactions of two groups were compared before and after treatment.Results The total effective rate in observation group was 87.5％ (35/40),which were significantly higher than 50.0％ (20/40) of the control group,there were significant difference (x2 =13.09,P ＜ 0.05).In the observation group,after treatment,the left ventricular ejection fraction (LVEF) and average hourly urine output were significantly higher than those of before treatment (t =26.40,3.22,all P ＜ 0.05),and the brain natriuretic peptide (BNP) concentration levels were significantly lower than those of before treatment (t =14.11,all P ＜ 0.05).The control group before and after treatment LVEF,BNP and changes in urine levels were not significantly.Conclusion In the treatment of heart failure caused by acute non-ST-segment elevation myocardial infarction,the recombinant human brain natriuretic peptide (rhBNP) has significant effect,feasible and safe with few side effects,which has a high clinical value.

Objective To assess the association between HLA-DQA 1 alleles and systemic lupus erythematosus (SLE) in populations of Uygur nationality in Xinjiang Uygur Autonomous Region.Methods Polvmerase chain reactionsequence-specific primers (PCR-SSP) were used to analyze the HLA-DQA1 gene in 56 patients with SLE and 54 unrelated healthy controls of Uygur nationalitv in Xinjiang Uygur Autonomous Region.Results Compared with the heahhv controls, the SLE patients showed signifieantly increased frequency of HLA-DQA1*0302 (x2 =10.032, P =0.004), but decreased frequency ofHLA-DQA1*0101 (x2 =5.676, P=0.017).Conclusion HLA-DQA1*0302 may be a susceptibility gene, while HLA-DQA1*0101 may be a protective factor for SLE in populations of Uygur nationality in Xinjiang Uygur Autonomous Region.

Objective To recognize the features of the ligamentum teres hepatic(LTH) in multi-slice spiral CT,and to preliminarily explore its clinical value.Methods A total of 130 patients without liver disease underwent abdominal scanning with a 16-slice spiral CT scanner.LTHs,as focused points,were observed by using postprocessing techniques such as multi-planar reformation(MPR),slab maximum intensity projection(slab-MIP) and slab volume rendering(slab-VR).Results The LTH was detected by multi-slice spiral CT in 90.8%(118/130).On these oblique-sagittal MPR,slab-MIP and slab-VR images,LTH presentedas slightly high density smooth cord-like structure in accordance with its anatomical morphology and position.The mean diameter of fissure portion of normal LTH was(5.44?0.96) mm(range 3.2-7.2 mm) measured at its middle region,and that of free portion of normal LTH was(3.43?0.91) mm(range 1.6-6.0 mm).Conclusions Multi-slice spiral CT demonstrates the LTH clearly,and has a potentialto evaluate the LTH before abdominal operation in which the LTH is to be used as an autogenous repairmaterial.

Objective To evaluate the role of via-anal ileus tube drainage in the therapy of acute low colorectal obstruction caused by cancer in elderly patients.Methods Totally 31 elderly patients with acute obstruction caused by colorectal cancer were treated by this modality.With the help of colonscopy and radiograph,the ileus tube was inserted into the proximal bowel of the obstructive side via anus.Then we irrigated and drain through the tube.At the same time we observed the patients' symptoms,abdominal girth,plain abdominal radiograph.After the relief of the acute obstruction,operations were processed.Results After 7-14 days treatment,the acute obstruction was relieved and emergency operation was avoided.The cure rate of 1-staged resection was 93.5％ (29/31),the rate of 1-staged anastomosis was 68.9％ (20/29),the rate of complication was 19.4％ (6/31),no anastomotic leakage and no perioperative death occurred.Conclusions The via-anal tube drainage in the treatment of the elderly patients with acute obstruction is safe,effective,and the trauma is small.It would be the first choice to relieve the acute obstruction in future.

Objective To analyze the clinical and pathological characteristics of breast carcinoma in elderly female patients and to offer the standard clinical diagnosis and treatment for breast carcinoma.Methods A total of 178 patients admitted to Breast Center of Beijing Hospital from January to December 2011 were enrolled in this retrospective study.Patients were divided into non-elderly group and elderly group.Clinical features,imaging diagnosis,operation patterns and pathological diagnosis were recorded.The data were analyzed by using SAS 9.1 software.Results The patients diagnosed as breast carcinomas in BI-RAD 4-5 categories by mammography were much more in the elderly group than in the non-elderly group (82.1％ vs.63.0％,P＜0.05).The diagnostic accuracy rates of ultrasonography and mammography in the elderly group were comparable(P＞ 0.05),while in the non-elderly group,the diagnostic accuracy rate of ultrasonography was higher than that of mammography (P＜0.05).The incidence of concurrent diseases was higher in the elderly group than in the non-elderly group (P＜0.05).The patients undergoing breast resection operation were much more in the elderly group than in the non-elderly group (14 cases vs.3 cases,P＜0.05).The pathological diagnosis analysis showed that there were no significant differences in the number of ER-positive cells and PR-positive cells,tumor size,histological grade,pathological stage between the two groups (all P＞0.05).The incidence of lymph node metastasis was lower in the elderly group than in the non-elderly group (P＜0.05).The percentage of Ki-67 positive cells was lower in the elderly group than in the non-elderly group (P＜0.05).The percentage of CerbB-2 strongly positive (+++) cells was lower in the elderly group than in the non-elderly group (5.1 ％ vs.24％,P＜0.05).The invasive ductal carcinoma was the major histological type of breast cancer in both two groups,but there were more mixed type and other type carcinomas in the elderly group than in the non-elderly group (P＜0.05).Conclusions Ultrasonograpy and mammography have better sensitivity for breast cancer in elderly female patients.The surgical protocols are often influenced because more concurrent diseases have occurred in the elderly.There are significant differences in some prognostic factors between the two groups,which may imply a better prognosis in elderly patients.

Aim To establish a LC-MS/MS method for the determination of pirfenidone(BT)and its major metabolite 5-carboxy-pirfenidone(SBT)in human plasma.Methods Human plasma samples containing BT and SBT,as well as their corresponding deuterium-labeled internal standards pirfenidone-d5(dBT)and 5-carboxy-pirfenidone-d5(dSBT),were precipitated using methanol.Chromatographic separation was carried out on an Agilent ZORBAX SB C18(3.0 mm×100 mm,3.5 μm)column with the mobile phase of water(0.5％formic acid)and acetonitrile(50/50).The detection of analytes was performed on a tandem mass system equipped with an electrospray ionization source in positive ion mode using multiple-reaction monitoring.The MS/MS ion transitions monitored were m/z 185.958→77.1 for BT,m/z 215.944→77.0 for SBT,m/z 190.965→81.1 for dBT and m/z 220.948→99.1 for dSBT.Results There was no remarkable interference in blank solvent,plasma,and there was no mutual interference between analytes or internal standards.The proposed method showed good linearity over the concentration range of 0.020 59～25.14 mg·L-1 for BT and 0.016 73～20.42 mg·L-1 for SBT.The intra-batch and inter-batch precision and accuracy were proved to be acceptable.Human samples kept stable after 4 h at room temperature,the three freeze-thaw cycles and 10,29 and 52 days at-70 ℃,and the processed samples remained stable after 24 h in the autosampler.The average extraction recovery and matrix effect were precise,reproducible and acceptable.Conclusion Our current LC-MS/MS method is proved to be sensitive,accurate and convenient,and could be suitable for the clinical pharmacokinetic studies of BT-related preparations.

Objective:To detect the immune response in mice inoculated by DNA vaccine expressing chimeric gene gag gp120 of HIV 1.Methods:After BALB/C mice were immunized by the eukaryotic expression plasmid pVAXGE,the numbers of T lymphocyte subgroups of spleen,the killing rate of specific CTL from the immunized mice and the titers of serum antibodies during the different time were observed.Results:The lymphocytes of the immunized group by recombinant plasmid pVAXGE proliferated,the specific CTL killing rate of spleen was higher significantly than those of the control group( P