Objective To observe the efficacy of underwater partial body-weight-supported treadmill training in repairing spinal cord injury (SCI) and its relationship with spinal nerve plasticity. Methods A total of 40 Sprague-Dawley rats were randomly divided into five groups: a sham model group, a model control group, an underwater training group, a partial body-weight-supported treadmill training (PBWSTT) group and an underwater PBWSTT group. A rat model of SCI was induced by contusion of the T10 segment with a Multicenter Animal Spinal Cord Injury Study (MASCIS) impactor. One week post-operation, different rehabilitation strategies, such as free exercise in water, BWSTT and underwater PBWSTT, were administered to the rats in the underwater training groups for 8 weeks.Those in the sham model group and model control group were given no training. The Basso, Beattie and Bresnahan (BBB) locomotor rating scale and a climbing test were used to evaluate the recovery of hindlimb locomotor function.The expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in the spinal cords was detected with immunohistochemical methods. Results Nine weeks post-operation, hindlimb locomotor function had improved significantly more in the underwater PBWSTT group than in underwater training group or the BWSTT group.The expression of BDNF in the 3 training groups was significantly higher than in the model control group, though there was no significant difference among the 3 training groups. The expression of NT-3 in the underwater PBWSTT group increased more significantly than in the BWSTT group, however there was no significant difference between the underwater PBWSTT group and the underwater training group. Conclusion Underwater PBWSTT can promote the recovery of hindlimb locomotor function in rats after SCI, probably through increasing the expression of BDNF and NT-3 and thus promoting neural plasticity in the spinal cord.

Objective: To understand the prevalence and epidemiological features of anemia during pregnancy in China. Methods: The literature retrieval was conducted by using China Biology Medicine disc (CBMdisc), China National Knowledge Infrastructure (CNKI), Wanfang Data, China Science and Technology Journal Database (CSTJ), PubMed, Cochrane Library and Embase database. The time was from January 2012 to October 2017, and the language was limited to Chinese and English. The Chinese and corresponding English search terms included pregnancy, anemia, epidemiology and prevalence. Results: A total of 28 papers were included. The total number of participants investigated was 58 752, with 11 588 cases with anemia during pregnancy identified. The overall prevalence of anemia during pregnancy was 19.9% (95%CI: 16.3%-23.5%). The prevalence varied in anemia with different levels. The prevalence of mild, moderate and severe anemia were 15.9% (95%CI: 11.7%-20.2%), 5.7% (95%CI: 4.5%-6.8%) and 1.3% (95%CI: 0.8%-1.8%) respectively. The prevalence of mild anemia was highest and the prevalence of severe anemia was lowest. The prevalence of anemia increased with gestational weeks. The prevalence of anemia at the first, second and third trimester of pregnancy were 10.1% (95%CI: 6.2%-14.0%), 26.7% (95%CI: 18.0%-35.5%) and 28.1% (95%CI: 21.0%-35.1%) respectively. Both degree and gestational week subgroup analysis showed a significant difference among groups (P<0.05). There were no statistically significant differences among subgroups of cut-off year, region, resident area, age, education level and parity (P>0.05). Conclusion The prevalence of anemia during pregnancy in China had remained at a low level during the past 5 years. The prevalence of mild anemia was higher than that of moderate and severe anemia. The prevalence of anemia in women at the second and third trimester of pregnancy was higher than that at the first trimester of pregnancy. Women at the second and third trimester of pregnancy should be attached a great importance in the prevention and control of anemia during pregnancy.

Objective To investigate the effect of genistein on the proliferation,migration and invasion of prostate cancer cells and its molecular mechanism.Methods Prostate cancer LNCaP and CWR22RV1 cells were divided into the control group(conventional culture)and the experimental group(50μmol/L genistein treatment).The effect of genistein on the proliferation of prostate cancer cells were analyzed by MTT assay.The effect of genistein on the migration and invasion of prostate cancer cells were analyzed by cell scratch assay and Transwell assay.The protein levels of epithelial interstital transformation(EMT)intermediate markers E-Cadherin,N-Cadherin,Vimentin,and tumor stem cell markers CD44 and Oct-4 were detected by Western blot assay.Results MTT assay showed that genistein could inhibit the proliferation of prostate cancer cells.The scratch closure rates of LNCaP and CWR22RV1 cells were significantly reduced in the experimental group compared with those in the control group,and the number of cells passing through the Transwell membrane was significantly reduced(P＜0.05).Western blot assay showed that genistein could down-regulate the expression levels of N-Cadherin,Vimentin,CD44 and Oct4 in prostate cancer cells,and up-regulate the expression of E-Cadherin in epithelial cells(P＜0.01).Conclusion Genistein reduces the dryness of prostate cancer cells by inhibiting the EMT process,thus reducing the proliferation,migration and invasion of prostate cancer cells.