Objective To evaluate clinical importance of high ligation combined with endovenous laser treat-ment(EVLT)for great sapenous varicose veins patients with diabetes,to provide a reference for clinical treatment. Methods 65 great sapenous varicose veins patients with diabetes were selected,and they were set as the observation group with treatment of high ligation combined with EVLT;while 50 great sapenous varicose veins patients without diabetes were selected during the same period,they were set as the control group under the same treatment.Clinical effect and complication were compared between the two groups.Results The wound cure rate of the observation group was 96.92%,surgery time was (42.94 ±7.93)min,hospital stay time was (6.87 ±1.74)d,there were no statistical differences compared with the control group (χ2 =1.003,P =0.174;t =0.994,P =0.254;t =0.883,P =0.317);The complication rate of the observation group was 18.46%,that of control group was 18.00%,there was no statistical difference between the two groups(χ2 =1.114,P =0.097).Conclusion With control of blood sugar,high ligation combined with endovenous laser treatment can get a good result and safety performance for great sapenous varicose veins patients with diabetes.

Objective:To investigate the intervention effect of vitamin C for the treatment to diabetes patients with diabetes and gastric ulcer.Methods: 53 cases of patients with diabetes combined with gastric ulcer were selected as the research subjects, and were divided into a control group(23) and a intervention group(30). The patients in two groups were given the treatment of reducing blood sugar and anti-HP triple therapy. The intervention group was given additionally diet intervention via eating vitamin C. The blood sugar, blood lipid levels, gastric ulcer index and inflammatory markers of the two groups were reviewed six months later by helicobacter pylori type detector. And the statistical analysis was performed.Results: After treatment, the blood sugar, blood lipid levels, gastric ulcer index and inflammatory markers of the two groups were significantly improved (t=1.23,t=1.35,t=2.63,t=1.07,t=0.77,P<0.05), except for the HP cure rate (P>0.05). Other indexes of the intervention group are significantly better than the control group (t=1.45,t=1.67,t=2.33, t=1.55,P<0.05).Conclusion:Vitamin C play a very good role in the treatment and prevention for the blood sugar, the blood lipids, gastric function and inflammation of patients with diabetes and gastric ulcer, and helicobacter pylori type detector can simplify the detection of helicobacter pylori.

Health education is not only the first step to health management,but also an important health intervention.It has an important significance for promoting the development of health management because it runs through the whole process of health management.However,because the health management has just started in our country,it faces many challenges when putting the health education into effect in the health management process.From the organizational structure,management system,resources,education content and method of the implementing body,this paper summarized eight problems existing in the implementation.This paper tried putting forward corresponding countermeasures,and hoped to be helpful to the further development of health education.

Objective To explore the effectiveness of bilevel positive airway pressure(Bipap)ventilation via nasal/ mouth mask in patients with respiratory failure secondary to the acute exacerbation of chronic obstructive pulmonary diseases(COPD).Methods 40 patients were randomly submitted either to standard therapy(consisting of medical,oxygen and physical therapy)plus BiPAP or to standard therapy.Clinical manifestation were comparatively analyzed before and after 4 hours and 7 days of treatment in each group.Results After 4 hours and 7 days of treatment by BiPAP,patients showed a significant improvement in PaCO_2,and clinical manifestation,and there was a significant difference between the two groups.Conclusion Bipap ventilation is effective in patients with respiratory failure during acute exacerbation of COPD.

Objective To investigate the histological injury as well as the risk factors for the course of HBeAg-negative chronic hep-atitis B virus( HBV)-infected patients with persistently normal alanine aminotransferase ( ALT) levels ( PNAL) . Methods Ninty four patients of HBeAg-negative chronic HBV-infected with PNAL were performed liver puncturing. The ALT, HBV markers, and liver histo-logical changes were detected. The gender, age, family history, HBV-DNA and HBsAg levels were compared between different patients with liver inflammation and fibrosis. And the Logistic model was used during the analysis of independent risk factor. Results Among 94 cases, patients with inflammatory necrosis classification≥G2 accounted for 74. 5%, fibrosis grading≥S2 accounted for 27. 7%. Patients in the group of inflammatory necrosis classification≥G2 was older than the group of < G2, and the ALT level of≥G2 group was higher, as well as a higher HBV-DNA. What is more,Patients in the group of inflammatory necrosis classification ≥G2 were mainly male, and were associated with a family history of hepatitis B. Patients in the group of fibrosis stage ≥S2 was older than < S2 group, and the ALT level of fibrosis stage ≥S2 group was higher. And those patients were often associated with a family history of hepatitis B, but there was no significant difference among the HBV-DNA level and gender constitution. HBV-DNA and family history were the independent risk fac-tors of liver inflammation necrosis≥G2, and family history was the only independent risk factor of fibrosis stage≥S2. Conclusion The patients of HBeAg-negative chronic HBV-infected with PNAL, regardless of the ALT and HBV-DNA, must have a liver biopsy. The pa-tients with high level of HBV-DNA and with a family history of hepatitis B should be performed liver puncturing accordingly.

By means of HPLC,the changes of endogenous hormones in the process of releasing dormancy in Fritillaria thunbergii Miq. seeds were determined. The results indicated that the content of GA3 had a process of increase while the content of ABA was reduced in general. The content of IAA showed a downward -plateau-upward-downward curve in the process of releasing dormancy. When the dormancy of the seeds were released attemperatures 8~10℃ and 3~5℃,changing pattern of each of the three endogenous hormones were similar,but the duration spanning each change were different.

Objective To study the effects of various media, salt intensity, and organic components on the growth of Salvia miltiorrhiza adventitious roots and the synthesis of tanshinone ⅡA and protoca-techuic aldehyde. MethodsThe adventitious roots were obtained through tissue culture by manipulation of various media, salt intensity, and organic components and the contents of tanshinone ⅡA and protocatechuic aldehyde were determined by HPLC. ResultsThe effect of media MS, LS, B5, White, and SH on adventitious roots of S. miltiorrhiza was observed. Adventitious roots grew better under high salt intensity while secondary metabolite biosynthesis was accelerated under low salt intensity in MS basal medium.The reciprocity of five organic components had significant effect on root growth; glycin favored the synthesis of tanshinone ⅡA; scarcity of one of inosital, glycin, VB1, and VB6 inhibited the synthesis of protocatechuic aldehyde. ConclusionMS Basal medium is used for adventitious root culture. The results show that salt intensity and organic components have significant effects on adventitious root culture of S. miltiorrhiza and secondary melabolite synthesis.

Objective To study the effects of carbon, nitrogen, and phosphate sources on the growth of Salvia miltiorrhiza adventitious roots and the contents of tanshinone ⅡA and protocatechuic aldehyde. Methods The adventitious roots were obtained through tissue culture by manipulation of carbon, nitrogen, and phosphate sources and the contents of tanshinone ⅡA and protocatechuic aldehyde were determined by HPLC. Results Carbon, nitrogen, and phosphate sources were necessary for the culture of S. miltiorrhiza adventitious roots. The highest times of root multiplication were achieved at sucrose level of 30 g/L after 20 d culture, 60 g/L sucrose and low level sucrose were favorable for biosyntheses of tanshinone ⅡA and protocatechuic aldehyde, respectively. The highest root yield and tanshinone ⅡA content on day 25 were obtained by intermittent sugar adding during cultivation, and the production of adventitious roots and tanshinone ⅡA were 2.3-and 2.4-fold compared with those of control, respectively. The maximum root growth rate, contents of tanshinone ⅡA and protocatechuic aldehyde were achieved while NH4+-NO3-was 1∶4, 1∶4, and 1∶1, respectively when concentration of total nitrogen source was kept at 60 mmol/L. To compare with the control group, changing of KH2PO4 concentration could favor for the adventilious root growth, but high KH2PO4 concentration inhibited tanshinone ⅡA biosynthesis. ConclusionThe results show that various carbon, nitrogen, and phosphate sources have the significant effects on adventitious root culture of S. miltiorrhiza. The best carbon source and its concentration, nitrogen and phosphate sources for the growth of S. miltiorrhiza adventitious root and the synthesis of secondary metabolite are confirmed.

Initially,the renin-angiotensin system (RAS) was considered to play an important role in regulating cardiovascular function and maintaining the balance of water and electrolyte.Based on this,several targeted drugs in the treatment of hypertension were developed.With the large-scale clinical apphcation of these drugs,RAS inhibitors are found to has a significant inhibitory effect on some of the tumor development,which reveals the RAS function in cell proliferation,differentiation,angiogenesis and tumor occurrence.In this paper,the important physiological ftmction of RAS in tumor occurrence and development were reviewed.

Twelve specimens of genital warts were excised from 12 patients and divided into three parts. One part was untreated, the second and the third part were treated with CO_2 laser and microwave respectively. HPV DNA was amplified and detected in 100% of untreated specimens (HPV16 and HPV11 in six patients each), in 83% and 50% of specimens treated with CO_2 laser and microwave respectively. There was a significant difference in the detection rates between untreated and microwave treated specimens (X~2=4.18, P