Objective To observe the changes of serum ET-1,TNF-α and VEGF in patients with hypertensive disorder complicating pregnancy ( HDCP),explore the significance of cytokines in HDCP and the relationship between cytokines and HDCP.Methods The serum levels of ET-1,TNF-α and VEGF in 60 patients with HDCP and 30 normal pregnant women were detected by ELISA.Results The serum levels of ET-1,TNF-o in HDCP group were higher than those of the control group( all P ＜0.05).The serum level of VEGF in HDCP group was lower than that of the control group ( P ＜ 0.05).Negative correlation was observed between VEGF and ET-1,TNF-α in HDCP group (P ＜ 0.05).Conclusion The serum levels of ET-1,TNF-α increased significantly,while the serum level of VEGF decreased.ET-1,TNF-α and VEGF may play important roles in ipathogenesis and development of HDCP.

Objective: To investigate the suppressive effects of immature dendritic cells derived from donor bone on mice allogenic teeth rejection. Methods: immature dendritic cells derived from mice bone marrow were generated with 50 U/ml granulocyte-macrophage colony-stimulating factor; the phenotype were analyzed by flow cytometry. 2?106 imDC per mouse were administered to the recipients through tail vein 7 days before allogenic teeth transplantation. The suppressive effects of imDC on transplantation rejection were observed through histological section. Results: The infiltration of lymph cell and Inflammatory reaction in allogenic teeth transplantation group were reduced after administration of imDC to the recipients.Conclusion:The allogenic teeth rejection could be lessened by imDC.

Objective:To demonstrate the effect of cytokine with different concentration in mouse bone marrow-derived immature DCs and find the optimal cytokine dose for DC culture.Methods:mouse bone marrow-derived immature DCs were cultured in the presence of low,moderate and high dose of GM-CSF cytokine with or without IL-4.The morphology of cells were observed and the phenotype were detected.Results:On day 7,DCs in low dose group had the least quantity,small cell process and low expression of CD80,CD86 and MHC class II.The quantity of DCs increased if cultured with IL-4;DCs in moderate dose group and high dose group were more than that in low dose group and the expression of CD80,CD86 and MHC classⅡwere higher,but it is no difference between moderate dose group and high dose group,however cultured with or without IL-4.Conclusion:Higher purity of immature DCs could be get when cultured with low dose of GM-CSF.It was no difference of yield and purity of immature DCs when cultured with moderate or high dose of GM-CSF whether or not combine IL-4.

OBJECTIVE:To explore the general regulation and characteristics of adverse drug reactions(ADR)induced by ti-cagrelor,and provide reference for clinical rational drug use. METHODS:672 patients received ticagrelor in our hospital from Dec. 2013 to Jun. 2015 were followed-up,and the situation was analyzed and summarized after taking drug. RESULTS:In the 672 patients,78(11.6%)patients sutfered from 108 cases of ADR,the ratio of male to female was 5.5∶1,there was statistical signifi-cance (P<0.05);patients mostly aged 51-60 years (37.2%);the mainly involved organs/systems were respiratory system (41.7%),and skin and its appendages (38.0%),the main clinical manifestations were minor bleeding (78.7%),dyspnea (17.6%) and bradycardia (2.8%);no severe ADR was found. CONCLUSIONS:Ticagrelor had good safety and tolerability,but due to its shorter time to market in China and absense of evidence-based medicine for large-scale clinical trials in Asian population, clinic should strengthen medication monitoring to reduce the ADR and ensure drug safety.

Objective:To systematically analyze the ADRs caused by azithromycin,and provide references for its safe and rational meidication.Method:Based on the literature search in Chinese Journal Full-text Database(CNKI) between 1994 and 2007,139 cases of adverse drug reactions caused by azithromycin were collected and analyzed.Result:The ADRs caused by azithromycin could be categorized into 7 types,and the most frequent one was allergy.Conclusion:Adverse reactions monitoring of azithromycin should be strengthened to ensure its safe and rational meidication.

ObjectiveTostudythetumorpatientswithcentralvenouscatheterrelatedbloodstreaminfection ( CRBSI) risk factors related to monitoring the clinical guidance value and nursing intervention results.Methods Through to the cancer center venipuncture and maintain hand hygiene,aseptic technique and in the process of CRBSI infection monitoring,analysis of risk factors for infection rates change,effective nursing intervention measures for im-plementation.Results CRBSI infection rate was down from 9.31‰ in July 2013 to 0 in september and October 2013.Conclusion Process and results of monitoring is conducive to timely find CRBSI infection risk factors give cor-responding nursing intervention,control and reduce CRBSI rates.

Objective: To discuss the urgent need to improve the handling capacity of the outpatient information system in our hospital under the context that the patient registration is not available after the daily outpatient exceeding about 4,500 people times and that the outpatient demand is rapidly growing. Methods: To analyze all factors which constraint outpatient demands, develop relevant solutions, and update the outpatient information system in phases so as to ensure the normal medical work. Results:After the initial updating, the handling capacity of the system is up to from 4,500 to 13,000 people times, meeting the patient’s need; in the future, the complete updating will lead to a handling capacity to 19,000 people times. Conclusion: The comprehensive consideration shall be given to the building of the hospital information system, including the scalability of the system during its design.

Pulse wave velocity (PWV) is one of typical indicators to evaluate arterial stiffness.About the gender difference of pulse wave velocity,some controversies exist.In this paper,several factors affecting the differences such as height,aging,sex steroids,which affect the arterial structure and function and bring the gender difference,are reviewed relatively in detail.In addition,several aspects including measurement accuracy,analyzing method and the characteristics of sample population,which may induce the difference of PWV results,are also briefly reviewed.Meanwhile,some existing controversies are indicated objectively to further possible research interests in this field.

Copeptin,the C-terminal portion of provasopressin,is a novel neurohormone of the arginine vasopressin (AVP)system,and is known to be co-released with AVP from hypothalamus.As a surrogate marker of the AVP system,copeptin has gradually replaced AVP in several clinical studies largely due to its structural and methodological advantages.Copeptin has been regarded as a marker of non-specific stress response.In recent years,copeptin attracts more and more attention especially in cardiovascular conditions (heart failure and acute coronary syndromes).The present review primarily focuses on copeptin detection,its general advantages,and its potential clinical value in a variety of cardiovascular conditions.

Diabetes can affect structure and function of brain in several aspects, but diabetic cognitive impairment is often ignored. The pathogenesis of diabetes associated cognitive impairment is complicated. Factors such as glucotoxicity, lipotoxicity, insulin resistance, hypoglycaemia and disturbance of Ca2 homeostasis may play important roles. Besides conventional diabetes therapy, new methods still need to be explored.