Objective:To demonstrate the effect of cytokine with different concentration in mouse bone marrow-derived immature DCs and find the optimal cytokine dose for DC culture.Methods:mouse bone marrow-derived immature DCs were cultured in the presence of low,moderate and high dose of GM-CSF cytokine with or without IL-4.The morphology of cells were observed and the phenotype were detected.Results:On day 7,DCs in low dose group had the least quantity,small cell process and low expression of CD80,CD86 and MHC class II.The quantity of DCs increased if cultured with IL-4;DCs in moderate dose group and high dose group were more than that in low dose group and the expression of CD80,CD86 and MHC classⅡwere higher,but it is no difference between moderate dose group and high dose group,however cultured with or without IL-4.Conclusion:Higher purity of immature DCs could be get when cultured with low dose of GM-CSF.It was no difference of yield and purity of immature DCs when cultured with moderate or high dose of GM-CSF whether or not combine IL-4.

Objective: To investigate the suppressive effects of immature dendritic cells derived from donor bone on mice allogenic teeth rejection. Methods: immature dendritic cells derived from mice bone marrow were generated with 50 U/ml granulocyte-macrophage colony-stimulating factor; the phenotype were analyzed by flow cytometry. 2?106 imDC per mouse were administered to the recipients through tail vein 7 days before allogenic teeth transplantation. The suppressive effects of imDC on transplantation rejection were observed through histological section. Results: The infiltration of lymph cell and Inflammatory reaction in allogenic teeth transplantation group were reduced after administration of imDC to the recipients.Conclusion:The allogenic teeth rejection could be lessened by imDC.

Objective To observe the changes of serum ET-1,TNF-α and VEGF in patients with hypertensive disorder complicating pregnancy ( HDCP),explore the significance of cytokines in HDCP and the relationship between cytokines and HDCP.Methods The serum levels of ET-1,TNF-α and VEGF in 60 patients with HDCP and 30 normal pregnant women were detected by ELISA.Results The serum levels of ET-1,TNF-o in HDCP group were higher than those of the control group( all P ＜0.05).The serum level of VEGF in HDCP group was lower than that of the control group ( P ＜ 0.05).Negative correlation was observed between VEGF and ET-1,TNF-α in HDCP group (P ＜ 0.05).Conclusion The serum levels of ET-1,TNF-α increased significantly,while the serum level of VEGF decreased.ET-1,TNF-α and VEGF may play important roles in ipathogenesis and development of HDCP.

Objective:To systematically analyze the ADRs caused by azithromycin,and provide references for its safe and rational meidication.Method:Based on the literature search in Chinese Journal Full-text Database(CNKI) between 1994 and 2007,139 cases of adverse drug reactions caused by azithromycin were collected and analyzed.Result:The ADRs caused by azithromycin could be categorized into 7 types,and the most frequent one was allergy.Conclusion:Adverse reactions monitoring of azithromycin should be strengthened to ensure its safe and rational meidication.

OBJECTIVE:To explore the general regulation and characteristics of adverse drug reactions(ADR)induced by ti-cagrelor,and provide reference for clinical rational drug use. METHODS:672 patients received ticagrelor in our hospital from Dec. 2013 to Jun. 2015 were followed-up,and the situation was analyzed and summarized after taking drug. RESULTS:In the 672 patients,78(11.6%)patients sutfered from 108 cases of ADR,the ratio of male to female was 5.5∶1,there was statistical signifi-cance (P<0.05);patients mostly aged 51-60 years (37.2%);the mainly involved organs/systems were respiratory system (41.7%),and skin and its appendages (38.0%),the main clinical manifestations were minor bleeding (78.7%),dyspnea (17.6%) and bradycardia (2.8%);no severe ADR was found. CONCLUSIONS:Ticagrelor had good safety and tolerability,but due to its shorter time to market in China and absense of evidence-based medicine for large-scale clinical trials in Asian population, clinic should strengthen medication monitoring to reduce the ADR and ensure drug safety.

ObjectiveTostudythetumorpatientswithcentralvenouscatheterrelatedbloodstreaminfection ( CRBSI) risk factors related to monitoring the clinical guidance value and nursing intervention results.Methods Through to the cancer center venipuncture and maintain hand hygiene,aseptic technique and in the process of CRBSI infection monitoring,analysis of risk factors for infection rates change,effective nursing intervention measures for im-plementation.Results CRBSI infection rate was down from 9.31‰ in July 2013 to 0 in september and October 2013.Conclusion Process and results of monitoring is conducive to timely find CRBSI infection risk factors give cor-responding nursing intervention,control and reduce CRBSI rates.

Objective: To discuss the urgent need to improve the handling capacity of the outpatient information system in our hospital under the context that the patient registration is not available after the daily outpatient exceeding about 4,500 people times and that the outpatient demand is rapidly growing. Methods: To analyze all factors which constraint outpatient demands, develop relevant solutions, and update the outpatient information system in phases so as to ensure the normal medical work. Results:After the initial updating, the handling capacity of the system is up to from 4,500 to 13,000 people times, meeting the patient’s need; in the future, the complete updating will lead to a handling capacity to 19,000 people times. Conclusion: The comprehensive consideration shall be given to the building of the hospital information system, including the scalability of the system during its design.

Objective:To investigate the anti-tumor effect of tetrandrine on human liver cancer cell line 7402 in vitro.Effects of tetrandrine on proliferation and apoptosis of human liver cancer cell 7402 were observed.Methods:The effects of tetrandrine on proliferation of 7402 cells was observed by MTT assay and clonogenic assay.Apoptosis was observed by acridine orange(AO)/ethidium bromide(EB) Fluorescent staining?DNA gel electrophoresis and flow cytometry,and the expression of apoptosis related gene was analyzed by immunohistochemical staining method.Results:Tetrandrine inhibits the proliferation of 7402 cells in a dose dependent manner and induces apoptosis.Immunohistochemical staining showed that the expression of Bcl-2 was decreased and the expression of Bax was increasd in 7402 cells treated with tetrandrine.Conclusion:Tetrandrine inhibits the proliferation of 7402 in the dose dependent manner,and induces apoptosis.The antitumor effect of tetrandrine may be due to the regulation of the expressions of Bcl-2 and Bax.

Objective To explore the feasibility of laparoscopically assisted one-stage anorectoplasty for the treatment of intermediate imperforate anus. Methods Under laparoscopic visualization, the rectum was mobilized. Then the rectourethral (or rectovaginal) fistula was repaired laparoscopically (6 cases) or through perineal approach (4 cases). Afterwards a tunnel was made through the center of pelvic floor musculature by combined use of laparoscopic and perineal approach. The rectum was pull-through the perineum and anastomosed with skin. At Last the colostomy was closed. Results Laparoscopically assisted anorectal pull-through was successfully performed in all cases. The mean duration of laparoscopic dissection was 32.8 minutes (range, 25~78 minutes). The mean blood loss was 5.3 ml (range, 2~10 ml). No blood transfusion was needed. The intraurethral catheter or cystostomy tube was removed on the 11th postoperative day. No intraoperative complication was encountered and all infants had uneventful postoperative recovery without wound infection. During a follow-up for 3~66 months (mean, 45 months), the fecal continence evaluation showed excellent in 8 cases and good in 3 cases. Conclusions Laparoscopically assisted anorectoplasty is an effective and minimally invasive technique for the treatment of intermediate imperforate anus. This technique has advantages of simple performance, accurate identification of the center of the pelvic floor musculature, and low infection rate.

Objective To explain the material foundation of anti-inflammatory and analgesic effects of Jinxuan Zhike Xunxi San. Methods The foot swelling experiments in rat and xylene-induced ear edema experiments in mouse were adopted to observe the anti-inflammatory effects of the material extracted and purified from Jinxuan Zhike Xunxi San. The hot plate-induced pain experiment for studying the analgesic effect was established. Results The material of supercritical CO2 extract, saponins and flavonoids extract and minerals in Jinxuan Zhike Xunxi San had a inhibiting effect on egg-induced foot swelling in rat, saponins and flavonoids extract had a distinct inhibiting effect on xylene-induced ear edema in mouse. Every extract had obvious analgesic effects on hot plate-induced pain and significantly increased the threshold of pain. Conclusion The active ingredient groups of Jinxuan Zhike Xunxi San are the material of supercritical CO2 extract, saponins and flavonoids extract and minerals.