Objective] To analyze the effect of powder for ascending and descending on patients with different cough. [Method] The treatment group patients had still cough with conventional western medicine for two weeks, and initial treatment group patients had not any treatment after cough. All these patients were treated with powder for ascending and descending according to different Chinese traditional medicine syndromes cough. [Results] There were 49 patients, and 17 cases were the initial diagnosed group and 32 patients were the after treatment group. Patients with the improvement of the symptoms of cough and throat were effective in 15 cases(88.2%), while in patients with the improvement for sputum symptoms in initial group were 16 cases(94.1%), and 27 case(84.4%) in treatment group. It had no statistically significant difference between the two groups(χ2=0.006, P=0.940). Cough concrete improvement between the two groups showed no significant difference(χ2 =0.006, P =0.465). Between the two groups cough, throat and sputum symptoms overall improvement rate was 87.8%(43/49), no significant difference between the two groups(P>0.05). [Conclusion] Regardless of the treatment patients or untreated patients, treatment with powder for ascending and descending had good effect in improvement of the symptoms of cough, and the improvement of throat and sputum was effective, too.

Triphala consists of Terminalia chebula Retz, Terminalia bellirica (Gaertn.) Roxb. and Phyllanthus emblica L, which are made into Dasanguo Powder. Triphala is rich in a variety of chemical components, including tannins, phenolic acids, triterpenoids and flavonoids. The content of tannin is abundant in Triphala, which are often used as the main indicators of analysis. Modern research found that Triphala has a variety of pharmacological activities such as prevention of gastrointestinal diseases, antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and so on. This paper briefly summarized the chemical constituents and pharmacological effects of Triphala, combining the relevant national and international literature in recent years to provide reference for the development and further study of Triphala.

This paper aimed at investigating the water-soluble constituents of Terminalia billirica (Gaert.) Roxb.Compounds were isolated and purified by Diaion HP-20, Sephadex LH-20, Toyopearl HW-40, MCI gel CHP 20 P and ODS column chromatographies. The chemical structures were elucidated according to spectral data and physicochemical properties. The results showed that ten compounds were isolated and identified as corilagin, salicoside, methyl-α-Darabinofuranoside, 1, 4-dimethyl-β-d-fructopyranose, β-fructopyranose, β-fructofuranose gallic acid, ellagic acid, 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranonside, Chebulagic acid. Except compound 7 and 8, others compounds are separated from Terminalia billirica (Gaert.) Roxb for the first time, and except corilgin, the other compounds are separated from Terminalia for the first time.

The aim of this work was to identify and analyze the chemical constituents of Terminalia chebular Retz by establishing high performance liquid chromatography-linear ion trap/electrostatic field orbitrap combined high resolution mass spectrometry (HPLC-LTQ-Orbitrap MS) technique. With the application of C18 reverse phase column (4.6 mm×250 mm, 5 μm), gradient elution was performed with methanol and water (0.2％ acetic acid) as mobile phase.In negative ionization mode, the data of LTQ-Orbitrap was collected. Accurate molecular mass of molecular ion peaks and fragment ions provided by the high-resolution mass spectrometry were compared with the literature and the reference substance to determine the possible structure of the compound. The results indicated that 62 compounds from the extract of Terminalia chebular Retz. were identified, including acids and hydrolysable tannins (60), triterpenoids (2) . There were14 compounds were detected from this specie for the first time. It is proved as an effective method to provide reference for Chemical composition mass spectrometry and the quality control in further phytochemical studies of Terminalia chebular Retz.

The spectrophotometric method was established for the determination of total tannin content in gardenia medicinal materials and tannins, and HPLC method was applied to simultaneously determine gallic acid, punical glucoside A, methyl gallate, punny glucoside B, corilagin, pentagalloyl glucose and the content of ellagic acid. Taking gallic acid as a reference substance, a phosphomolybdate tungstic acid colorimetric method (Pharmacopoeia method) was used to determine the total tannin content. With the chromatographic column Agilent ZORBAX SB-Aq-C18 (250 × 4.6 mm, 5 microns), mobile phase for phosphoric acid (0.1％), methanol, water flow rate of 1 ml/min, column temperature 30℃, detection wavelength of 270 nm, gradient elution, 7 kinds of component contents in myrobalan medicinal materials were measured at the same time. The result showed that the average content of total tannins of Radix Scutellariae was 30.31％, RSD was 0.55％; the average recovery rate was 99.70％, and the RSD was 21.97％ (n = 6) . In the scorpion medicinal materials, gallic acid, punical glucoside A, punny glucoside B, methyl gallate, punny glucoside B, creatin, pentagalloglucose and ellagic acid have a good linear relationship in their respective ranges. The recovery rate of the sample is between100.10％ and 102.77％. The establishment of the determination method of total tannin, gallic acid, punical glucoside, methyl gallate, corridain, pentagalloglucose and ellagic acid in the scorpion medicinal materials is simple and accurate, which has strong specificity and can be used for quality control of Terminalia chebular.

This paper was aimed to establish the HPLC fingerprint of Terminalia chebular Retz The analysis was performed on Agilent ZORBAX SB-Aq C18 column (4.6×250 mm, 5 μm) . The mobile phase was 0.1％ phosphate watermethanol solution with gradient elution. The flow rate was 1 mL·min-1. The temperature was maintained at 35℃. The detection wavelength was 270 nm. The injection volume was 20 μL. The chromatographic data of 10 samples was analyzed by similarity of chromatographic data, SPSS software and SIMCA software. The results showed that there were17 common peaks in the diagram. The similarity of 10 samples was between 0.984 and 0.999, which showed that there was no significant difference among these chemical components. The samples were classified into four types according to cluster analysis, which shared the similar results as the principal components analysis (PCA) and partial least sq uares analysis (PLS-DA) . VIP analysis result showed that peak 5 (methyl gallate), 11 and 1 (gallic acid), 10, 12 may be the main chromatographic peaks to identify the samples. It was concluded that chemometrics analysis could be used to analyze, find and distinguish the marker ingredients of Terminalia chebular Retz. This method was accurate and stable, which can provide a reference for the quality control and evaluation of Terminalia chebular Retz.

This paper was aimed to establish the HPLC fingerprint of T.Billerica (Gaertn.) Roxb.tannin fraction.The analysis was performed on Atlantic T3 (250 mm × 4.6 mm,5 μm) C18 column.The mobile phase was acetonitrile-0.2％ glacial acetic acid aqueous solution with gradient elution.The flow rate was 1.0 mL·min-1.The injection size was 20 μL.The temperature was maintained at 30℃.Eleven batches of T.Billerica (Gaertn.) Roxb.tannin fraction of chromatographic data was analyzed by similarity of chromatographic data,SPSS software and SIMCA software.There were 20 common peaks in the diagram.The similarity analysis of 11 samples revealed that the similarities were between 0.832 and 0.973.Only the similarity of tannin parts from Xinjiang was below 0.9.The cluster analysis classified the tannin parts into 3 types,which shared the similar results as the principal components analysis (PCA).PLS-DA found that peak 1,13 and 14 may be the main chromatographic peaks to identify tannins fraction.The HPLC-MSn information of 14 compounds in T.billerica (Gaertn.) Roxb.was summarized.It was concluded that chemometrics analysis method can be used to analyze the HPLC fingerprint of T.billerica (Gaertn.) Roxb.tannin fraction.This method was rapid,simple,and reproducible.It can be used as one of the effective methods for the quality control of T.billerica (Gaertn.) Roxb.tannin fraction.

This article was aimed to study the stability of tannin parts in Phyllanthus emblica L.in artificial gastric and intestinal juice,in order to provide a foundation for in vivo studies of Phyllanthus emblica L.HPLC-UV was used to determine the contents of main ingredients.The results showed that stability of main ingredients of tannin parts in Phyllanthus emblica L.,including gallic acid (GA),corilagin and ellagic acid (EA),in the artificial gastric juice.The content change was not obvious.The residual content was within 100％.The half-life was above 90 h.They were unstable in the artificial intestinal juice.The content was first increased and then decreased.The residual content was 100-300％.The half-life was above 10 h.It was concluded that the tannin parts in Phyllanthus emblica L.was stable in artificial gastric juice.And content changes of main components were not obvious.The tannin parts in Phyllanthus emblica L.were unstable in artificial intestinal juice.The main ingredients were first increased and then decreased.It was speculated that macromolecule can hydrolyze tannin part into small molecules.Hnwever,as time increases,all components may be decomposed.

This study was aimed to optimize the extraction method for total polyphenol from Phyllanthus emblica through multi-target orthogonal experiment,in order to provide data reference for its industrial production.The comprehensive evaluation indexes included the extraction yield,extraction percentage of total polyphenol,chebulagic acid,gallic acid,mucic acid-2-O-gallate,were verified.The ethanol concentration,solid-liquid ratio and extraction time were crucial indexes for orthogonal design.The results showed that the best extraction process was using 10 times dosage 70％ ethanol,to extract the medical material three time,90 minutes for every time.It was concluded that the extraction technology was reliable.This method was stable,quick and simple.It laid a fundamental foundation for the extraction method exploration.

High performance liquid chromatography (HPLC) fingerprint technology is an important means of quality control of traditional Chinese medicine (TCM) preparations,which can reflect the whole process of raw materials as well as the whole process and quality of finished products.It has become the primary quality control method of modern TCM preparations.In this paper,recent advances in HPLC fingerprints and their combination techniques in the quality control of TCM preparations were reviewed,which provided the basis for quality control of TCM preparations.